Heavy metal resistance mechanisms in actinobacteria for survival in AMD contaminated soils

Autores
Schmidt, Andre; Haferburg, Götz; Siñeriz Louis, Manuel; Merten, Dirk; Büchel, Georg; Kothe, Erika
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A site in the former uranium mining area of Eastern Thuringia near Ronneburg was investigated with regard to effects of acid mine drainage (AMD) on reactive transport and bioattenuation. Processes involved in this attenuation might include physico-chemical reactions in reactive transport as well as activities of microorganisms for bioattenuation. In order to test the influence of the soil microbes, a mapping was carried out including both hydrogeochemical and microbiological parameters. Mapping of contamination was performed along the banks of a creek in a 900 m stretch in 50 m steps by hydrogeochemical analysis of water extracts of soil samples, while general microbial activity was scored by examining soil respiration. The soil samples with high heavy metal load did show low soil respiration as a parameter for microbial activity and plating revealed minimal counts for spore producing bacteria at these contaminated locations. Actinobacteria strains isolated from adjacent locations revealed high levels of resistance as well as high numbers of resistant strains. Specific responses in actinobacteria were investigated after isolation from each of the 18 measuring points along the creek. Specific adaptation strategies and high yields of (intra)cellular heavy metal retention could be seen. Several strategies for coping with the high heavy metal contents are further discussed and genes for proteins expressed specifically under high nickel concentration were identified by two-dimensional gel electrophoresis. © 2005 Elsevier GmbH. All rights reserved.
Fil: Schmidt, Andre. Friedrich Schiller University; Alemania
Fil: Haferburg, Götz. Friedrich Schiller University; Alemania
Fil: Siñeriz Louis, Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Friedrich Schiller University; Alemania
Fil: Merten, Dirk. Friedrich Schiller University; Alemania
Fil: Büchel, Georg. Friedrich Schiller University; Alemania
Fil: Kothe, Erika. Friedrich Schiller University; Alemania
Materia
Actinobacteria
Amd
Bioavailable Heavy Metals
Bioremediation
Heavy Metal Resistance
Molecular Biology
Proteome Analysis
Soil Extract
Streptomycetes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/56810
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/56810
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Heavy metal resistance mechanisms in actinobacteria for survival in AMD contaminated soilsSchmidt, AndreHaferburg, GötzSiñeriz Louis, ManuelMerten, DirkBüchel, GeorgKothe, ErikaActinobacteriaAmdBioavailable Heavy MetalsBioremediationHeavy Metal ResistanceMolecular BiologyProteome AnalysisSoil ExtractStreptomyceteshttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1A site in the former uranium mining area of Eastern Thuringia near Ronneburg was investigated with regard to effects of acid mine drainage (AMD) on reactive transport and bioattenuation. Processes involved in this attenuation might include physico-chemical reactions in reactive transport as well as activities of microorganisms for bioattenuation. In order to test the influence of the soil microbes, a mapping was carried out including both hydrogeochemical and microbiological parameters. Mapping of contamination was performed along the banks of a creek in a 900 m stretch in 50 m steps by hydrogeochemical analysis of water extracts of soil samples, while general microbial activity was scored by examining soil respiration. The soil samples with high heavy metal load did show low soil respiration as a parameter for microbial activity and plating revealed minimal counts for spore producing bacteria at these contaminated locations. Actinobacteria strains isolated from adjacent locations revealed high levels of resistance as well as high numbers of resistant strains. Specific responses in actinobacteria were investigated after isolation from each of the 18 measuring points along the creek. Specific adaptation strategies and high yields of (intra)cellular heavy metal retention could be seen. Several strategies for coping with the high heavy metal contents are further discussed and genes for proteins expressed specifically under high nickel concentration were identified by two-dimensional gel electrophoresis. © 2005 Elsevier GmbH. All rights reserved.Fil: Schmidt, Andre. Friedrich Schiller University; AlemaniaFil: Haferburg, Götz. Friedrich Schiller University; AlemaniaFil: Siñeriz Louis, Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Friedrich Schiller University; AlemaniaFil: Merten, Dirk. Friedrich Schiller University; AlemaniaFil: Büchel, Georg. Friedrich Schiller University; AlemaniaFil: Kothe, Erika. Friedrich Schiller University; AlemaniaElsevier Gmbh, Urban & Fischer Verlag2005-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/56810Schmidt, Andre; Haferburg, Götz; Siñeriz Louis, Manuel; Merten, Dirk; Büchel, Georg; et al.; Heavy metal resistance mechanisms in actinobacteria for survival in AMD contaminated soils; Elsevier Gmbh, Urban & Fischer Verlag; Chemie Der Erde; 65; SUPPL. 1; 9-2005; 131-1440009-2819CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemer.2005.06.006info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0009281905000486info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:13Zoai:ri.conicet.gov.ar:11336/56810instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:14.157CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Heavy metal resistance mechanisms in actinobacteria for survival in AMD contaminated soils
title Heavy metal resistance mechanisms in actinobacteria for survival in AMD contaminated soils
spellingShingle Heavy metal resistance mechanisms in actinobacteria for survival in AMD contaminated soils
Schmidt, Andre
Actinobacteria
Amd
Bioavailable Heavy Metals
Bioremediation
Heavy Metal Resistance
Molecular Biology
Proteome Analysis
Soil Extract
Streptomycetes
title_short Heavy metal resistance mechanisms in actinobacteria for survival in AMD contaminated soils
title_full Heavy metal resistance mechanisms in actinobacteria for survival in AMD contaminated soils
title_fullStr Heavy metal resistance mechanisms in actinobacteria for survival in AMD contaminated soils
title_full_unstemmed Heavy metal resistance mechanisms in actinobacteria for survival in AMD contaminated soils
title_sort Heavy metal resistance mechanisms in actinobacteria for survival in AMD contaminated soils
dc.creator.none.fl_str_mv Schmidt, Andre
Haferburg, Götz
Siñeriz Louis, Manuel
Merten, Dirk
Büchel, Georg
Kothe, Erika
author Schmidt, Andre
author_facet Schmidt, Andre
Haferburg, Götz
Siñeriz Louis, Manuel
Merten, Dirk
Büchel, Georg
Kothe, Erika
author_role author
author2 Haferburg, Götz
Siñeriz Louis, Manuel
Merten, Dirk
Büchel, Georg
Kothe, Erika
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Actinobacteria
Amd
Bioavailable Heavy Metals
Bioremediation
Heavy Metal Resistance
Molecular Biology
Proteome Analysis
Soil Extract
Streptomycetes
topic Actinobacteria
Amd
Bioavailable Heavy Metals
Bioremediation
Heavy Metal Resistance
Molecular Biology
Proteome Analysis
Soil Extract
Streptomycetes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A site in the former uranium mining area of Eastern Thuringia near Ronneburg was investigated with regard to effects of acid mine drainage (AMD) on reactive transport and bioattenuation. Processes involved in this attenuation might include physico-chemical reactions in reactive transport as well as activities of microorganisms for bioattenuation. In order to test the influence of the soil microbes, a mapping was carried out including both hydrogeochemical and microbiological parameters. Mapping of contamination was performed along the banks of a creek in a 900 m stretch in 50 m steps by hydrogeochemical analysis of water extracts of soil samples, while general microbial activity was scored by examining soil respiration. The soil samples with high heavy metal load did show low soil respiration as a parameter for microbial activity and plating revealed minimal counts for spore producing bacteria at these contaminated locations. Actinobacteria strains isolated from adjacent locations revealed high levels of resistance as well as high numbers of resistant strains. Specific responses in actinobacteria were investigated after isolation from each of the 18 measuring points along the creek. Specific adaptation strategies and high yields of (intra)cellular heavy metal retention could be seen. Several strategies for coping with the high heavy metal contents are further discussed and genes for proteins expressed specifically under high nickel concentration were identified by two-dimensional gel electrophoresis. © 2005 Elsevier GmbH. All rights reserved.
Fil: Schmidt, Andre. Friedrich Schiller University; Alemania
Fil: Haferburg, Götz. Friedrich Schiller University; Alemania
Fil: Siñeriz Louis, Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Friedrich Schiller University; Alemania
Fil: Merten, Dirk. Friedrich Schiller University; Alemania
Fil: Büchel, Georg. Friedrich Schiller University; Alemania
Fil: Kothe, Erika. Friedrich Schiller University; Alemania
description A site in the former uranium mining area of Eastern Thuringia near Ronneburg was investigated with regard to effects of acid mine drainage (AMD) on reactive transport and bioattenuation. Processes involved in this attenuation might include physico-chemical reactions in reactive transport as well as activities of microorganisms for bioattenuation. In order to test the influence of the soil microbes, a mapping was carried out including both hydrogeochemical and microbiological parameters. Mapping of contamination was performed along the banks of a creek in a 900 m stretch in 50 m steps by hydrogeochemical analysis of water extracts of soil samples, while general microbial activity was scored by examining soil respiration. The soil samples with high heavy metal load did show low soil respiration as a parameter for microbial activity and plating revealed minimal counts for spore producing bacteria at these contaminated locations. Actinobacteria strains isolated from adjacent locations revealed high levels of resistance as well as high numbers of resistant strains. Specific responses in actinobacteria were investigated after isolation from each of the 18 measuring points along the creek. Specific adaptation strategies and high yields of (intra)cellular heavy metal retention could be seen. Several strategies for coping with the high heavy metal contents are further discussed and genes for proteins expressed specifically under high nickel concentration were identified by two-dimensional gel electrophoresis. © 2005 Elsevier GmbH. All rights reserved.
publishDate 2005
dc.date.none.fl_str_mv 2005-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/56810
Schmidt, Andre; Haferburg, Götz; Siñeriz Louis, Manuel; Merten, Dirk; Büchel, Georg; et al.; Heavy metal resistance mechanisms in actinobacteria for survival in AMD contaminated soils; Elsevier Gmbh, Urban & Fischer Verlag; Chemie Der Erde; 65; SUPPL. 1; 9-2005; 131-144
0009-2819
CONICET Digital
CONICET
url http://hdl.handle.net/11336/56810
identifier_str_mv Schmidt, Andre; Haferburg, Götz; Siñeriz Louis, Manuel; Merten, Dirk; Büchel, Georg; et al.; Heavy metal resistance mechanisms in actinobacteria for survival in AMD contaminated soils; Elsevier Gmbh, Urban & Fischer Verlag; Chemie Der Erde; 65; SUPPL. 1; 9-2005; 131-144
0009-2819
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemer.2005.06.006
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0009281905000486
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh, Urban & Fischer Verlag
publisher.none.fl_str_mv Elsevier Gmbh, Urban & Fischer Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269508810571776
score 13.13397

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