High-level resistance to gentamicin: Genetic transfer between Enterococcus faecalis isolated from food of animal origin and human microbiota

Autores
Sparo, Mónica Delfina; Urbizu, Lucia Paola; Solana, María Victoria; Pourcel, Natalia Gisela; Delpech, Gastón; Confalonieri, Alejandra; Ceci, M.; Sanchez Bruni, Sergio Fabian
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: To investigate the in vivo gene transfer of high-level gentamicin resistance (HLRG) from Enterococcus faecalis isolated from the food of animal origin to a human isolate, using a mouse model of intestinally colonized human microbiota. Methods and Results: In vitro study: The presence of plasmids involved in HLRG coding was investigated. After the conjugation experiment, the recipient strain, Ent. faecalis JH2-SS, acquired a plasmid responsible for HLRG [minimal inhibitory concentration (MIC) >800μgml -1], in a similar position to the donor cells. In vivo study: Seven BALB/c mice were dosed with ceftriaxone (400mgkg -1) and then inoculated with a dilution of 1/100 of human faeces (HFc). After 72h, Ent. faecalis JH2-SS (recipient) was inoculated and then, after a further 72h, the animals were given Ent. faecalis CS19, isolated from the food of animal origin, involved in HLRG (donor). The presence of transconjugant strains in HFc was subsequently recorded on a daily basis until the end of the experiment. The clonal relationship between Ent. faecalis and Escherichia coli in faeces was assessed by RAPD-PCR. Both the in vitro and in vivo studies showed that the receptor strain acquired a plasmid responsible for HLRG (MICs >800μgml -1), which migrated with a similar relative mobility value. Transconjugant strains were detected from 24h after the donor strain inoculation and persisted until the end of the experiment. Conclusions: The in vivo gene transfer of HLRG from Ent. faecalis strains, isolated from the food of animal origin, to human microbiota has been demonstrated in a mouse model. Significance and Impact of the Study: The complexity found on the therapeutic responses of invasive infectious diseases caused by Ent. faecalis facilitates the assessment of food of animal origin as a resistant pathogen reservoir. In addition, this study may contribute to the understanding of antimicrobials' resistance gene transfer between Ent. faecalis strains from food and human GI tract.
Fil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Urbizu, Lucia Paola. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Solana, María Victoria. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pourcel, Natalia Gisela. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Delpech, Gastón. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Confalonieri, Alejandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ceci, M.. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Sanchez Bruni, Sergio Fabian. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Conjugative Plasmid
Enterococcus Faecalis
Human Microbiota
Mice
Resistance
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66997
Acceder
id CONICETDig_5d2f60016c6aae203ae776df3f322f38
oai_identifier_str oai:ri.conicet.gov.ar:11336/66997
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling High-level resistance to gentamicin: Genetic transfer between Enterococcus faecalis isolated from food of animal origin and human microbiotaSparo, Mónica DelfinaUrbizu, Lucia PaolaSolana, María VictoriaPourcel, Natalia GiselaDelpech, GastónConfalonieri, AlejandraCeci, M.Sanchez Bruni, Sergio FabianConjugative PlasmidEnterococcus FaecalisHuman MicrobiotaMiceResistancehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Aims: To investigate the in vivo gene transfer of high-level gentamicin resistance (HLRG) from Enterococcus faecalis isolated from the food of animal origin to a human isolate, using a mouse model of intestinally colonized human microbiota. Methods and Results: In vitro study: The presence of plasmids involved in HLRG coding was investigated. After the conjugation experiment, the recipient strain, Ent. faecalis JH2-SS, acquired a plasmid responsible for HLRG [minimal inhibitory concentration (MIC) >800μgml -1], in a similar position to the donor cells. In vivo study: Seven BALB/c mice were dosed with ceftriaxone (400mgkg -1) and then inoculated with a dilution of 1/100 of human faeces (HFc). After 72h, Ent. faecalis JH2-SS (recipient) was inoculated and then, after a further 72h, the animals were given Ent. faecalis CS19, isolated from the food of animal origin, involved in HLRG (donor). The presence of transconjugant strains in HFc was subsequently recorded on a daily basis until the end of the experiment. The clonal relationship between Ent. faecalis and Escherichia coli in faeces was assessed by RAPD-PCR. Both the in vitro and in vivo studies showed that the receptor strain acquired a plasmid responsible for HLRG (MICs >800μgml -1), which migrated with a similar relative mobility value. Transconjugant strains were detected from 24h after the donor strain inoculation and persisted until the end of the experiment. Conclusions: The in vivo gene transfer of HLRG from Ent. faecalis strains, isolated from the food of animal origin, to human microbiota has been demonstrated in a mouse model. Significance and Impact of the Study: The complexity found on the therapeutic responses of invasive infectious diseases caused by Ent. faecalis facilitates the assessment of food of animal origin as a resistant pathogen reservoir. In addition, this study may contribute to the understanding of antimicrobials' resistance gene transfer between Ent. faecalis strains from food and human GI tract.Fil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Provincia de Buenos Aires; ArgentinaFil: Urbizu, Lucia Paola. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Solana, María Victoria. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pourcel, Natalia Gisela. Universidad Nacional del Centro de la Provincia de Buenos Aires; ArgentinaFil: Delpech, Gastón. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Confalonieri, Alejandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ceci, M.. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Sanchez Bruni, Sergio Fabian. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley Blackwell Publishing, Inc2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66997Sparo, Mónica Delfina; Urbizu, Lucia Paola; Solana, María Victoria; Pourcel, Natalia Gisela; Delpech, Gastón; et al.; High-level resistance to gentamicin: Genetic transfer between Enterococcus faecalis isolated from food of animal origin and human microbiota; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 54; 2; 2-2012; 119-1250266-8254CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1472-765X.2011.03182.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1472-765X.2011.03182.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:37:26Zoai:ri.conicet.gov.ar:11336/66997instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:37:26.972CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv High-level resistance to gentamicin: Genetic transfer between Enterococcus faecalis isolated from food of animal origin and human microbiota
title High-level resistance to gentamicin: Genetic transfer between Enterococcus faecalis isolated from food of animal origin and human microbiota
spellingShingle High-level resistance to gentamicin: Genetic transfer between Enterococcus faecalis isolated from food of animal origin and human microbiota
Sparo, Mónica Delfina
Conjugative Plasmid
Enterococcus Faecalis
Human Microbiota
Mice
Resistance
title_short High-level resistance to gentamicin: Genetic transfer between Enterococcus faecalis isolated from food of animal origin and human microbiota
title_full High-level resistance to gentamicin: Genetic transfer between Enterococcus faecalis isolated from food of animal origin and human microbiota
title_fullStr High-level resistance to gentamicin: Genetic transfer between Enterococcus faecalis isolated from food of animal origin and human microbiota
title_full_unstemmed High-level resistance to gentamicin: Genetic transfer between Enterococcus faecalis isolated from food of animal origin and human microbiota
title_sort High-level resistance to gentamicin: Genetic transfer between Enterococcus faecalis isolated from food of animal origin and human microbiota
dc.creator.none.fl_str_mv Sparo, Mónica Delfina
Urbizu, Lucia Paola
Solana, María Victoria
Pourcel, Natalia Gisela
Delpech, Gastón
Confalonieri, Alejandra
Ceci, M.
Sanchez Bruni, Sergio Fabian
author Sparo, Mónica Delfina
author_facet Sparo, Mónica Delfina
Urbizu, Lucia Paola
Solana, María Victoria
Pourcel, Natalia Gisela
Delpech, Gastón
Confalonieri, Alejandra
Ceci, M.
Sanchez Bruni, Sergio Fabian
author_role author
author2 Urbizu, Lucia Paola
Solana, María Victoria
Pourcel, Natalia Gisela
Delpech, Gastón
Confalonieri, Alejandra
Ceci, M.
Sanchez Bruni, Sergio Fabian
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Conjugative Plasmid
Enterococcus Faecalis
Human Microbiota
Mice
Resistance
topic Conjugative Plasmid
Enterococcus Faecalis
Human Microbiota
Mice
Resistance
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Aims: To investigate the in vivo gene transfer of high-level gentamicin resistance (HLRG) from Enterococcus faecalis isolated from the food of animal origin to a human isolate, using a mouse model of intestinally colonized human microbiota. Methods and Results: In vitro study: The presence of plasmids involved in HLRG coding was investigated. After the conjugation experiment, the recipient strain, Ent. faecalis JH2-SS, acquired a plasmid responsible for HLRG [minimal inhibitory concentration (MIC) >800μgml -1], in a similar position to the donor cells. In vivo study: Seven BALB/c mice were dosed with ceftriaxone (400mgkg -1) and then inoculated with a dilution of 1/100 of human faeces (HFc). After 72h, Ent. faecalis JH2-SS (recipient) was inoculated and then, after a further 72h, the animals were given Ent. faecalis CS19, isolated from the food of animal origin, involved in HLRG (donor). The presence of transconjugant strains in HFc was subsequently recorded on a daily basis until the end of the experiment. The clonal relationship between Ent. faecalis and Escherichia coli in faeces was assessed by RAPD-PCR. Both the in vitro and in vivo studies showed that the receptor strain acquired a plasmid responsible for HLRG (MICs >800μgml -1), which migrated with a similar relative mobility value. Transconjugant strains were detected from 24h after the donor strain inoculation and persisted until the end of the experiment. Conclusions: The in vivo gene transfer of HLRG from Ent. faecalis strains, isolated from the food of animal origin, to human microbiota has been demonstrated in a mouse model. Significance and Impact of the Study: The complexity found on the therapeutic responses of invasive infectious diseases caused by Ent. faecalis facilitates the assessment of food of animal origin as a resistant pathogen reservoir. In addition, this study may contribute to the understanding of antimicrobials' resistance gene transfer between Ent. faecalis strains from food and human GI tract.
Fil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Urbizu, Lucia Paola. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Solana, María Victoria. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pourcel, Natalia Gisela. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Delpech, Gastón. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Confalonieri, Alejandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ceci, M.. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Sanchez Bruni, Sergio Fabian. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Aims: To investigate the in vivo gene transfer of high-level gentamicin resistance (HLRG) from Enterococcus faecalis isolated from the food of animal origin to a human isolate, using a mouse model of intestinally colonized human microbiota. Methods and Results: In vitro study: The presence of plasmids involved in HLRG coding was investigated. After the conjugation experiment, the recipient strain, Ent. faecalis JH2-SS, acquired a plasmid responsible for HLRG [minimal inhibitory concentration (MIC) >800μgml -1], in a similar position to the donor cells. In vivo study: Seven BALB/c mice were dosed with ceftriaxone (400mgkg -1) and then inoculated with a dilution of 1/100 of human faeces (HFc). After 72h, Ent. faecalis JH2-SS (recipient) was inoculated and then, after a further 72h, the animals were given Ent. faecalis CS19, isolated from the food of animal origin, involved in HLRG (donor). The presence of transconjugant strains in HFc was subsequently recorded on a daily basis until the end of the experiment. The clonal relationship between Ent. faecalis and Escherichia coli in faeces was assessed by RAPD-PCR. Both the in vitro and in vivo studies showed that the receptor strain acquired a plasmid responsible for HLRG (MICs >800μgml -1), which migrated with a similar relative mobility value. Transconjugant strains were detected from 24h after the donor strain inoculation and persisted until the end of the experiment. Conclusions: The in vivo gene transfer of HLRG from Ent. faecalis strains, isolated from the food of animal origin, to human microbiota has been demonstrated in a mouse model. Significance and Impact of the Study: The complexity found on the therapeutic responses of invasive infectious diseases caused by Ent. faecalis facilitates the assessment of food of animal origin as a resistant pathogen reservoir. In addition, this study may contribute to the understanding of antimicrobials' resistance gene transfer between Ent. faecalis strains from food and human GI tract.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66997
Sparo, Mónica Delfina; Urbizu, Lucia Paola; Solana, María Victoria; Pourcel, Natalia Gisela; Delpech, Gastón; et al.; High-level resistance to gentamicin: Genetic transfer between Enterococcus faecalis isolated from food of animal origin and human microbiota; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 54; 2; 2-2012; 119-125
0266-8254
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66997
identifier_str_mv Sparo, Mónica Delfina; Urbizu, Lucia Paola; Solana, María Victoria; Pourcel, Natalia Gisela; Delpech, Gastón; et al.; High-level resistance to gentamicin: Genetic transfer between Enterococcus faecalis isolated from food of animal origin and human microbiota; Wiley Blackwell Publishing, Inc; Letters in Applied Microbiology; 54; 2; 2-2012; 119-125
0266-8254
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1472-765X.2011.03182.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1472-765X.2011.03182.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.976206

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