Evaluation of SDF-1/CXCR4 expression in human HER2/NEU positive breast cancer samples

Autores
Martínez, María de Los Ángeles; Larroza, Silvana Beatriz; Gomez Pescie, Mariana; Dos Santos Antola, Lorena; Zimmermann, Maria Carla
Año de publicación
2016
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Stromal Derived Factor-1 (SDF-1) is a chemokine whose membrane receptor CXCR4 can be found in breast tumor and the metastatic sites of the primary tumor. The signaling pathway of this chemokine and its receptor is involved and is capable to induce directional migration of cells due to a chemokine gradient. Recently, it has been observed that CXCR4 works as a mediator in the migration of tumor cells to specific organs and t it could be correlated with expression of the Human Epidermal )rowth Factor Receptor-2 (HER2/neu) in breast tumors. HER2/neu is a transmembrane protein, also known as ErbB-2, which has been implicated in many types of human cancers, specifically breast cancer, in which it is used as a prognostic factor. We hypothesized that the overexpression of HER2/neu activates the SDF-1/CXCR4 axis in HER2/neu positive breast tumors, and that transactivation enhances tumor progression, malignancy and metastasis capacity. The aim of this work was to investigate the association between SDF-1/CXCR4 and HER2/neu expression in breast cancer paraf- fin sections. We carried out an RT-qPCR of human breast tumor paraffin samples to determine the SDF-1 and CXCR4 expression. We, additionally, performed the same SDF-1/CXCR4 RT-qPCR in some of the metastatic sites of the primary tumor. Also, analysis of HER2/neu expression in these samples were determined by immunohistochemistry. Most HER2/neu positive breast tumor paraffin samples were found to express high levels of either SDF-1 of CXCR4. Some of the metastatic sites presented higher levels of this chemokine and its receptor expression, while others presented no expression at all. Further investigation is needed, particularly in HER2/neu negative samples, in order to achieve acceptable information to validate our hypothesis.
Fil: Martínez, María de Los Ángeles. Universidad Nacional del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Larroza, Silvana Beatriz. Universidad Nacional del Nordeste; Argentina
Fil: Gomez Pescie, Mariana. Universidad Nacional del Nordeste; Argentina
Fil: Dos Santos Antola, Lorena. Universidad Nacional del Nordeste. Facultad de Medicina. Cátedra de Farmacología; Argentina
Fil: Zimmermann, Maria Carla. Universidad Nacional del Nordeste. Facultad de Medicina. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
LXI Reunión Anual de la Sociedad Agentina de Investigación Clínica; LXIV Reunión Anual de la Sociedad Argentina de Inmunología; XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental; VII Reunión Anual de la Sociedad Argentina de Nanomedicina y V Congreso Nacional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Farmacología Experimental
Sociedad Argentina de Nanomedicina
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Materia
CANCER DE MAMA
SDF-1
HER2NEU
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/183832
Acceder
id CONICETDig_5c9310874994822bbbe32e3ca2541f31
oai_identifier_str oai:ri.conicet.gov.ar:11336/183832
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of SDF-1/CXCR4 expression in human HER2/NEU positive breast cancer samplesMartínez, María de Los ÁngelesLarroza, Silvana BeatrizGomez Pescie, MarianaDos Santos Antola, LorenaZimmermann, Maria CarlaCANCER DE MAMASDF-1HER2NEUhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Stromal Derived Factor-1 (SDF-1) is a chemokine whose membrane receptor CXCR4 can be found in breast tumor and the metastatic sites of the primary tumor. The signaling pathway of this chemokine and its receptor is involved and is capable to induce directional migration of cells due to a chemokine gradient. Recently, it has been observed that CXCR4 works as a mediator in the migration of tumor cells to specific organs and t it could be correlated with expression of the Human Epidermal )rowth Factor Receptor-2 (HER2/neu) in breast tumors. HER2/neu is a transmembrane protein, also known as ErbB-2, which has been implicated in many types of human cancers, specifically breast cancer, in which it is used as a prognostic factor. We hypothesized that the overexpression of HER2/neu activates the SDF-1/CXCR4 axis in HER2/neu positive breast tumors, and that transactivation enhances tumor progression, malignancy and metastasis capacity. The aim of this work was to investigate the association between SDF-1/CXCR4 and HER2/neu expression in breast cancer paraf- fin sections. We carried out an RT-qPCR of human breast tumor paraffin samples to determine the SDF-1 and CXCR4 expression. We, additionally, performed the same SDF-1/CXCR4 RT-qPCR in some of the metastatic sites of the primary tumor. Also, analysis of HER2/neu expression in these samples were determined by immunohistochemistry. Most HER2/neu positive breast tumor paraffin samples were found to express high levels of either SDF-1 of CXCR4. Some of the metastatic sites presented higher levels of this chemokine and its receptor expression, while others presented no expression at all. Further investigation is needed, particularly in HER2/neu negative samples, in order to achieve acceptable information to validate our hypothesis.Fil: Martínez, María de Los Ángeles. Universidad Nacional del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Larroza, Silvana Beatriz. Universidad Nacional del Nordeste; ArgentinaFil: Gomez Pescie, Mariana. Universidad Nacional del Nordeste; ArgentinaFil: Dos Santos Antola, Lorena. Universidad Nacional del Nordeste. Facultad de Medicina. Cátedra de Farmacología; ArgentinaFil: Zimmermann, Maria Carla. Universidad Nacional del Nordeste. Facultad de Medicina. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaLXI Reunión Anual de la Sociedad Agentina de Investigación Clínica; LXIV Reunión Anual de la Sociedad Argentina de Inmunología; XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental; VII Reunión Anual de la Sociedad Argentina de Nanomedicina y V Congreso Nacional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de Farmacología ExperimentalSociedad Argentina de NanomedicinaAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista Medicina2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/183832Evaluation of SDF-1/CXCR4 expression in human HER2/NEU positive breast cancer samples; LXI Reunión Anual de la Sociedad Agentina de Investigación Clínica; LXIV Reunión Anual de la Sociedad Argentina de Inmunología; XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental; VII Reunión Anual de la Sociedad Argentina de Nanomedicina y V Congreso Nacional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2016; 211-2111669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reunion-anualNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:40Zoai:ri.conicet.gov.ar:11336/183832instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:40.445CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of SDF-1/CXCR4 expression in human HER2/NEU positive breast cancer samples
title Evaluation of SDF-1/CXCR4 expression in human HER2/NEU positive breast cancer samples
spellingShingle Evaluation of SDF-1/CXCR4 expression in human HER2/NEU positive breast cancer samples
Martínez, María de Los Ángeles
CANCER DE MAMA
SDF-1
HER2NEU
title_short Evaluation of SDF-1/CXCR4 expression in human HER2/NEU positive breast cancer samples
title_full Evaluation of SDF-1/CXCR4 expression in human HER2/NEU positive breast cancer samples
title_fullStr Evaluation of SDF-1/CXCR4 expression in human HER2/NEU positive breast cancer samples
title_full_unstemmed Evaluation of SDF-1/CXCR4 expression in human HER2/NEU positive breast cancer samples
title_sort Evaluation of SDF-1/CXCR4 expression in human HER2/NEU positive breast cancer samples
dc.creator.none.fl_str_mv Martínez, María de Los Ángeles
Larroza, Silvana Beatriz
Gomez Pescie, Mariana
Dos Santos Antola, Lorena
Zimmermann, Maria Carla
author Martínez, María de Los Ángeles
author_facet Martínez, María de Los Ángeles
Larroza, Silvana Beatriz
Gomez Pescie, Mariana
Dos Santos Antola, Lorena
Zimmermann, Maria Carla
author_role author
author2 Larroza, Silvana Beatriz
Gomez Pescie, Mariana
Dos Santos Antola, Lorena
Zimmermann, Maria Carla
author2_role author
author
author
author
dc.subject.none.fl_str_mv CANCER DE MAMA
SDF-1
HER2NEU
topic CANCER DE MAMA
SDF-1
HER2NEU
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Stromal Derived Factor-1 (SDF-1) is a chemokine whose membrane receptor CXCR4 can be found in breast tumor and the metastatic sites of the primary tumor. The signaling pathway of this chemokine and its receptor is involved and is capable to induce directional migration of cells due to a chemokine gradient. Recently, it has been observed that CXCR4 works as a mediator in the migration of tumor cells to specific organs and t it could be correlated with expression of the Human Epidermal )rowth Factor Receptor-2 (HER2/neu) in breast tumors. HER2/neu is a transmembrane protein, also known as ErbB-2, which has been implicated in many types of human cancers, specifically breast cancer, in which it is used as a prognostic factor. We hypothesized that the overexpression of HER2/neu activates the SDF-1/CXCR4 axis in HER2/neu positive breast tumors, and that transactivation enhances tumor progression, malignancy and metastasis capacity. The aim of this work was to investigate the association between SDF-1/CXCR4 and HER2/neu expression in breast cancer paraf- fin sections. We carried out an RT-qPCR of human breast tumor paraffin samples to determine the SDF-1 and CXCR4 expression. We, additionally, performed the same SDF-1/CXCR4 RT-qPCR in some of the metastatic sites of the primary tumor. Also, analysis of HER2/neu expression in these samples were determined by immunohistochemistry. Most HER2/neu positive breast tumor paraffin samples were found to express high levels of either SDF-1 of CXCR4. Some of the metastatic sites presented higher levels of this chemokine and its receptor expression, while others presented no expression at all. Further investigation is needed, particularly in HER2/neu negative samples, in order to achieve acceptable information to validate our hypothesis.
Fil: Martínez, María de Los Ángeles. Universidad Nacional del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Larroza, Silvana Beatriz. Universidad Nacional del Nordeste; Argentina
Fil: Gomez Pescie, Mariana. Universidad Nacional del Nordeste; Argentina
Fil: Dos Santos Antola, Lorena. Universidad Nacional del Nordeste. Facultad de Medicina. Cátedra de Farmacología; Argentina
Fil: Zimmermann, Maria Carla. Universidad Nacional del Nordeste. Facultad de Medicina. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
LXI Reunión Anual de la Sociedad Agentina de Investigación Clínica; LXIV Reunión Anual de la Sociedad Argentina de Inmunología; XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental; VII Reunión Anual de la Sociedad Argentina de Nanomedicina y V Congreso Nacional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Farmacología Experimental
Sociedad Argentina de Nanomedicina
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
description Stromal Derived Factor-1 (SDF-1) is a chemokine whose membrane receptor CXCR4 can be found in breast tumor and the metastatic sites of the primary tumor. The signaling pathway of this chemokine and its receptor is involved and is capable to induce directional migration of cells due to a chemokine gradient. Recently, it has been observed that CXCR4 works as a mediator in the migration of tumor cells to specific organs and t it could be correlated with expression of the Human Epidermal )rowth Factor Receptor-2 (HER2/neu) in breast tumors. HER2/neu is a transmembrane protein, also known as ErbB-2, which has been implicated in many types of human cancers, specifically breast cancer, in which it is used as a prognostic factor. We hypothesized that the overexpression of HER2/neu activates the SDF-1/CXCR4 axis in HER2/neu positive breast tumors, and that transactivation enhances tumor progression, malignancy and metastasis capacity. The aim of this work was to investigate the association between SDF-1/CXCR4 and HER2/neu expression in breast cancer paraf- fin sections. We carried out an RT-qPCR of human breast tumor paraffin samples to determine the SDF-1 and CXCR4 expression. We, additionally, performed the same SDF-1/CXCR4 RT-qPCR in some of the metastatic sites of the primary tumor. Also, analysis of HER2/neu expression in these samples were determined by immunohistochemistry. Most HER2/neu positive breast tumor paraffin samples were found to express high levels of either SDF-1 of CXCR4. Some of the metastatic sites presented higher levels of this chemokine and its receptor expression, while others presented no expression at all. Further investigation is needed, particularly in HER2/neu negative samples, in order to achieve acceptable information to validate our hypothesis.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/183832
Evaluation of SDF-1/CXCR4 expression in human HER2/NEU positive breast cancer samples; LXI Reunión Anual de la Sociedad Agentina de Investigación Clínica; LXIV Reunión Anual de la Sociedad Argentina de Inmunología; XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental; VII Reunión Anual de la Sociedad Argentina de Nanomedicina y V Congreso Nacional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2016; 211-211
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/183832
identifier_str_mv Evaluation of SDF-1/CXCR4 expression in human HER2/NEU positive breast cancer samples; LXI Reunión Anual de la Sociedad Agentina de Investigación Clínica; LXIV Reunión Anual de la Sociedad Argentina de Inmunología; XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental; VII Reunión Anual de la Sociedad Argentina de Nanomedicina y V Congreso Nacional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2016; 211-211
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reunion-anual
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269174848552960
score 13.13397

Publicaciones similares