HER2/neu protein expression and fine needle breast aspiration from Argentinean patients with non-palpable breast lesions

Autores
Domínguez, Wendy Gabriela; Nardi, Héctor; Montero, Héctor; Vincent, Esteban; Corte, María Marta; Balogh, Gabriela Andrea
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of this pilot project was to investigate whether the breast fine needle aspiration (FNA) technique is a useful tool for determining the increased risk of breast cancer in patients with non-palpable breast lesions. FNA is a minimally invasive technique that isolates mammary epithelial cells from breast cells in the suspicious region. In this study, two FNA samples were collected from 12 patients. The level of HER2/neu expression at the mRNA level (in serum) was measured in each patient. As gene amplification is characteristic of cancer cells and may assist in diagnosis and prognostic assessment, it is crucial that gene amplification of HER2/neu in patients with non-palpable breast lesions is compared to breast biopsy results. In serum, the level of HER2/neu was determined by ELISA assay. Gene amplification was determined by PCR and confirmed by IHC employing monoclonal ERRB2 in the FNA sample. The results indicate that FNA has a good correlation with breast biopsy. FNA combined with mammographic imaging is a strong tool for determining favorable treatment options for patients.
Fil: Domínguez, Wendy Gabriela. Texas A&M University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Fil: Nardi, Héctor. Hospital Interzonal Dr José Penna. Departamento de Ginecología. Bahía Blanca; Argentina
Fil: Montero, Héctor. Hospital Español. Departamento de Ginecología; Argentina
Fil: Vincent, Esteban. Hospital de la Asociación Médica de Bahía Blanca. Departamento de Ginecología. Bahía Blanca; Argentina
Fil: Corte, María Marta. Laboratorio de Patología. Bahía Blanca; Argentina
Fil: Balogh, Gabriela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Materia
BREAST FINE NEEDLE ASPIRATION
GENE EXPRESSION
HER2/NEU
NON-PALPABLE BREAST CARCINOMA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/15967
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/15967
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling HER2/neu protein expression and fine needle breast aspiration from Argentinean patients with non-palpable breast lesionsDomínguez, Wendy GabrielaNardi, HéctorMontero, HéctorVincent, EstebanCorte, María MartaBalogh, Gabriela AndreaBREAST FINE NEEDLE ASPIRATIONGENE EXPRESSIONHER2/NEUNON-PALPABLE BREAST CARCINOMAhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The objective of this pilot project was to investigate whether the breast fine needle aspiration (FNA) technique is a useful tool for determining the increased risk of breast cancer in patients with non-palpable breast lesions. FNA is a minimally invasive technique that isolates mammary epithelial cells from breast cells in the suspicious region. In this study, two FNA samples were collected from 12 patients. The level of HER2/neu expression at the mRNA level (in serum) was measured in each patient. As gene amplification is characteristic of cancer cells and may assist in diagnosis and prognostic assessment, it is crucial that gene amplification of HER2/neu in patients with non-palpable breast lesions is compared to breast biopsy results. In serum, the level of HER2/neu was determined by ELISA assay. Gene amplification was determined by PCR and confirmed by IHC employing monoclonal ERRB2 in the FNA sample. The results indicate that FNA has a good correlation with breast biopsy. FNA combined with mammographic imaging is a strong tool for determining favorable treatment options for patients.Fil: Domínguez, Wendy Gabriela. Texas A&M University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; ArgentinaFil: Nardi, Héctor. Hospital Interzonal Dr José Penna. Departamento de Ginecología. Bahía Blanca; ArgentinaFil: Montero, Héctor. Hospital Español. Departamento de Ginecología; ArgentinaFil: Vincent, Esteban. Hospital de la Asociación Médica de Bahía Blanca. Departamento de Ginecología. Bahía Blanca; ArgentinaFil: Corte, María Marta. Laboratorio de Patología. Bahía Blanca; ArgentinaFil: Balogh, Gabriela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; ArgentinaSpandidos Publ Ltd2010-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/15967Domínguez, Wendy Gabriela; Nardi, Héctor; Montero, Héctor; Vincent, Esteban; Corte, María Marta; et al.; HER2/neu protein expression and fine needle breast aspiration from Argentinean patients with non-palpable breast lesions; Spandidos Publ Ltd; Experimental and Therapeutic Medicine; 1; 4; 1-7-2010; 597-6021792-09811792-1015enginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3445870/info:eu-repo/semantics/altIdentifier/doi/10.3892/etm_00000094info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:40Zoai:ri.conicet.gov.ar:11336/15967instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:40.879CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv HER2/neu protein expression and fine needle breast aspiration from Argentinean patients with non-palpable breast lesions
title HER2/neu protein expression and fine needle breast aspiration from Argentinean patients with non-palpable breast lesions
spellingShingle HER2/neu protein expression and fine needle breast aspiration from Argentinean patients with non-palpable breast lesions
Domínguez, Wendy Gabriela
BREAST FINE NEEDLE ASPIRATION
GENE EXPRESSION
HER2/NEU
NON-PALPABLE BREAST CARCINOMA
title_short HER2/neu protein expression and fine needle breast aspiration from Argentinean patients with non-palpable breast lesions
title_full HER2/neu protein expression and fine needle breast aspiration from Argentinean patients with non-palpable breast lesions
title_fullStr HER2/neu protein expression and fine needle breast aspiration from Argentinean patients with non-palpable breast lesions
title_full_unstemmed HER2/neu protein expression and fine needle breast aspiration from Argentinean patients with non-palpable breast lesions
title_sort HER2/neu protein expression and fine needle breast aspiration from Argentinean patients with non-palpable breast lesions
dc.creator.none.fl_str_mv Domínguez, Wendy Gabriela
Nardi, Héctor
Montero, Héctor
Vincent, Esteban
Corte, María Marta
Balogh, Gabriela Andrea
author Domínguez, Wendy Gabriela
author_facet Domínguez, Wendy Gabriela
Nardi, Héctor
Montero, Héctor
Vincent, Esteban
Corte, María Marta
Balogh, Gabriela Andrea
author_role author
author2 Nardi, Héctor
Montero, Héctor
Vincent, Esteban
Corte, María Marta
Balogh, Gabriela Andrea
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BREAST FINE NEEDLE ASPIRATION
GENE EXPRESSION
HER2/NEU
NON-PALPABLE BREAST CARCINOMA
topic BREAST FINE NEEDLE ASPIRATION
GENE EXPRESSION
HER2/NEU
NON-PALPABLE BREAST CARCINOMA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The objective of this pilot project was to investigate whether the breast fine needle aspiration (FNA) technique is a useful tool for determining the increased risk of breast cancer in patients with non-palpable breast lesions. FNA is a minimally invasive technique that isolates mammary epithelial cells from breast cells in the suspicious region. In this study, two FNA samples were collected from 12 patients. The level of HER2/neu expression at the mRNA level (in serum) was measured in each patient. As gene amplification is characteristic of cancer cells and may assist in diagnosis and prognostic assessment, it is crucial that gene amplification of HER2/neu in patients with non-palpable breast lesions is compared to breast biopsy results. In serum, the level of HER2/neu was determined by ELISA assay. Gene amplification was determined by PCR and confirmed by IHC employing monoclonal ERRB2 in the FNA sample. The results indicate that FNA has a good correlation with breast biopsy. FNA combined with mammographic imaging is a strong tool for determining favorable treatment options for patients.
Fil: Domínguez, Wendy Gabriela. Texas A&M University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Fil: Nardi, Héctor. Hospital Interzonal Dr José Penna. Departamento de Ginecología. Bahía Blanca; Argentina
Fil: Montero, Héctor. Hospital Español. Departamento de Ginecología; Argentina
Fil: Vincent, Esteban. Hospital de la Asociación Médica de Bahía Blanca. Departamento de Ginecología. Bahía Blanca; Argentina
Fil: Corte, María Marta. Laboratorio de Patología. Bahía Blanca; Argentina
Fil: Balogh, Gabriela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
description The objective of this pilot project was to investigate whether the breast fine needle aspiration (FNA) technique is a useful tool for determining the increased risk of breast cancer in patients with non-palpable breast lesions. FNA is a minimally invasive technique that isolates mammary epithelial cells from breast cells in the suspicious region. In this study, two FNA samples were collected from 12 patients. The level of HER2/neu expression at the mRNA level (in serum) was measured in each patient. As gene amplification is characteristic of cancer cells and may assist in diagnosis and prognostic assessment, it is crucial that gene amplification of HER2/neu in patients with non-palpable breast lesions is compared to breast biopsy results. In serum, the level of HER2/neu was determined by ELISA assay. Gene amplification was determined by PCR and confirmed by IHC employing monoclonal ERRB2 in the FNA sample. The results indicate that FNA has a good correlation with breast biopsy. FNA combined with mammographic imaging is a strong tool for determining favorable treatment options for patients.
publishDate 2010
dc.date.none.fl_str_mv 2010-07-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/15967
Domínguez, Wendy Gabriela; Nardi, Héctor; Montero, Héctor; Vincent, Esteban; Corte, María Marta; et al.; HER2/neu protein expression and fine needle breast aspiration from Argentinean patients with non-palpable breast lesions; Spandidos Publ Ltd; Experimental and Therapeutic Medicine; 1; 4; 1-7-2010; 597-602
1792-0981
1792-1015
url http://hdl.handle.net/11336/15967
identifier_str_mv Domínguez, Wendy Gabriela; Nardi, Héctor; Montero, Héctor; Vincent, Esteban; Corte, María Marta; et al.; HER2/neu protein expression and fine needle breast aspiration from Argentinean patients with non-palpable breast lesions; Spandidos Publ Ltd; Experimental and Therapeutic Medicine; 1; 4; 1-7-2010; 597-602
1792-0981
1792-1015
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3445870/
info:eu-repo/semantics/altIdentifier/doi/10.3892/etm_00000094
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.publisher.none.fl_str_mv Spandidos Publ Ltd
publisher.none.fl_str_mv Spandidos Publ Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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