Application of DV-SIA manifold for determination of thiocyanate ions in human saliva samples

Autores
Acebal, Carolina Cecilia; Sklená?ová, Hana; Skrlíková, Jana; Srámková, Ivana; Andruch, Vasil; Balogh, Ioseph S.; Solich, Petr
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An automated, simple and inexpensive double-valve sequential injection analysis (DV-SIA) spectrophotometic method with online liquid-liquid extraction, for the determination of thiocyanate has been developed. The method has been based on the formation of an ion associate between thiocyanate and Astra Phloxine in acidic medium, and the subsequent extraction with amylacetate. The absorbance of the extracted ion associate was measured at 550 nm. The calibration function was linear in the range 0.05-0.50 mmol L-1 and the regression equation was A = (1.887 ± 0.053) [SCN- mmol L-1] + (0.037 ± 0.014) with a correlation coefficient of 0.995. The precision of the proposed method was evaluated by the relative standard deviation (RSD) values at two concentration levels: 0.20 and 0.50 mmol L-1. The obtained results were 1.0 and 2.8%, respectively, for the intra-day precision, and 4.2 and 3.8%, respectively for the inter-day precision. The calculated detection limit was 0.02 mmol L-1. The developed method has been successfully applied for determining thiocyanate ions in human saliva samples. © 2012 Elsevier B.V. All rights reserved.
Fil: Acebal, Carolina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina. Charles University; República Checa
Fil: Sklená?ová, Hana. Charles University; República Checa
Fil: Skrlíková, Jana. Charles University; República Checa. University of Pavol Jozef Šafárik; República Checa
Fil: Srámková, Ivana. Charles University; República Checa
Fil: Andruch, Vasil. University of Pavol Jozef Šafárik; República Checa
Fil: Balogh, Ioseph S.. College of Nyíregyház; Hungría
Fil: Solich, Petr. Charles University; República Checa
Materia
Biological Samples
Dv-Sia
Extraction
Thiocyanate
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/63933
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/63933
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Application of DV-SIA manifold for determination of thiocyanate ions in human saliva samplesAcebal, Carolina CeciliaSklená?ová, HanaSkrlíková, JanaSrámková, IvanaAndruch, VasilBalogh, Ioseph S.Solich, PetrBiological SamplesDv-SiaExtractionThiocyanatehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1An automated, simple and inexpensive double-valve sequential injection analysis (DV-SIA) spectrophotometic method with online liquid-liquid extraction, for the determination of thiocyanate has been developed. The method has been based on the formation of an ion associate between thiocyanate and Astra Phloxine in acidic medium, and the subsequent extraction with amylacetate. The absorbance of the extracted ion associate was measured at 550 nm. The calibration function was linear in the range 0.05-0.50 mmol L-1 and the regression equation was A = (1.887 ± 0.053) [SCN- mmol L-1] + (0.037 ± 0.014) with a correlation coefficient of 0.995. The precision of the proposed method was evaluated by the relative standard deviation (RSD) values at two concentration levels: 0.20 and 0.50 mmol L-1. The obtained results were 1.0 and 2.8%, respectively, for the intra-day precision, and 4.2 and 3.8%, respectively for the inter-day precision. The calculated detection limit was 0.02 mmol L-1. The developed method has been successfully applied for determining thiocyanate ions in human saliva samples. © 2012 Elsevier B.V. All rights reserved.Fil: Acebal, Carolina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina. Charles University; República ChecaFil: Sklená?ová, Hana. Charles University; República ChecaFil: Skrlíková, Jana. Charles University; República Checa. University of Pavol Jozef Šafárik; República ChecaFil: Srámková, Ivana. Charles University; República ChecaFil: Andruch, Vasil. University of Pavol Jozef Šafárik; República ChecaFil: Balogh, Ioseph S.. College of Nyíregyház; HungríaFil: Solich, Petr. Charles University; República ChecaElsevier Science2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/63933Acebal, Carolina Cecilia; Sklená?ová, Hana; Skrlíková, Jana; Srámková, Ivana; Andruch, Vasil; et al.; Application of DV-SIA manifold for determination of thiocyanate ions in human saliva samples; Elsevier Science; Talanta; 96; 7-2012; 107-1120039-9140CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.talanta.2012.01.021info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0039914012000276info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:34Zoai:ri.conicet.gov.ar:11336/63933instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:34.602CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Application of DV-SIA manifold for determination of thiocyanate ions in human saliva samples
title Application of DV-SIA manifold for determination of thiocyanate ions in human saliva samples
spellingShingle Application of DV-SIA manifold for determination of thiocyanate ions in human saliva samples
Acebal, Carolina Cecilia
Biological Samples
Dv-Sia
Extraction
Thiocyanate
title_short Application of DV-SIA manifold for determination of thiocyanate ions in human saliva samples
title_full Application of DV-SIA manifold for determination of thiocyanate ions in human saliva samples
title_fullStr Application of DV-SIA manifold for determination of thiocyanate ions in human saliva samples
title_full_unstemmed Application of DV-SIA manifold for determination of thiocyanate ions in human saliva samples
title_sort Application of DV-SIA manifold for determination of thiocyanate ions in human saliva samples
dc.creator.none.fl_str_mv Acebal, Carolina Cecilia
Sklená?ová, Hana
Skrlíková, Jana
Srámková, Ivana
Andruch, Vasil
Balogh, Ioseph S.
Solich, Petr
author Acebal, Carolina Cecilia
author_facet Acebal, Carolina Cecilia
Sklená?ová, Hana
Skrlíková, Jana
Srámková, Ivana
Andruch, Vasil
Balogh, Ioseph S.
Solich, Petr
author_role author
author2 Sklená?ová, Hana
Skrlíková, Jana
Srámková, Ivana
Andruch, Vasil
Balogh, Ioseph S.
Solich, Petr
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biological Samples
Dv-Sia
Extraction
Thiocyanate
topic Biological Samples
Dv-Sia
Extraction
Thiocyanate
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An automated, simple and inexpensive double-valve sequential injection analysis (DV-SIA) spectrophotometic method with online liquid-liquid extraction, for the determination of thiocyanate has been developed. The method has been based on the formation of an ion associate between thiocyanate and Astra Phloxine in acidic medium, and the subsequent extraction with amylacetate. The absorbance of the extracted ion associate was measured at 550 nm. The calibration function was linear in the range 0.05-0.50 mmol L-1 and the regression equation was A = (1.887 ± 0.053) [SCN- mmol L-1] + (0.037 ± 0.014) with a correlation coefficient of 0.995. The precision of the proposed method was evaluated by the relative standard deviation (RSD) values at two concentration levels: 0.20 and 0.50 mmol L-1. The obtained results were 1.0 and 2.8%, respectively, for the intra-day precision, and 4.2 and 3.8%, respectively for the inter-day precision. The calculated detection limit was 0.02 mmol L-1. The developed method has been successfully applied for determining thiocyanate ions in human saliva samples. © 2012 Elsevier B.V. All rights reserved.
Fil: Acebal, Carolina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina. Charles University; República Checa
Fil: Sklená?ová, Hana. Charles University; República Checa
Fil: Skrlíková, Jana. Charles University; República Checa. University of Pavol Jozef Šafárik; República Checa
Fil: Srámková, Ivana. Charles University; República Checa
Fil: Andruch, Vasil. University of Pavol Jozef Šafárik; República Checa
Fil: Balogh, Ioseph S.. College of Nyíregyház; Hungría
Fil: Solich, Petr. Charles University; República Checa
description An automated, simple and inexpensive double-valve sequential injection analysis (DV-SIA) spectrophotometic method with online liquid-liquid extraction, for the determination of thiocyanate has been developed. The method has been based on the formation of an ion associate between thiocyanate and Astra Phloxine in acidic medium, and the subsequent extraction with amylacetate. The absorbance of the extracted ion associate was measured at 550 nm. The calibration function was linear in the range 0.05-0.50 mmol L-1 and the regression equation was A = (1.887 ± 0.053) [SCN- mmol L-1] + (0.037 ± 0.014) with a correlation coefficient of 0.995. The precision of the proposed method was evaluated by the relative standard deviation (RSD) values at two concentration levels: 0.20 and 0.50 mmol L-1. The obtained results were 1.0 and 2.8%, respectively, for the intra-day precision, and 4.2 and 3.8%, respectively for the inter-day precision. The calculated detection limit was 0.02 mmol L-1. The developed method has been successfully applied for determining thiocyanate ions in human saliva samples. © 2012 Elsevier B.V. All rights reserved.
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/63933
Acebal, Carolina Cecilia; Sklená?ová, Hana; Skrlíková, Jana; Srámková, Ivana; Andruch, Vasil; et al.; Application of DV-SIA manifold for determination of thiocyanate ions in human saliva samples; Elsevier Science; Talanta; 96; 7-2012; 107-112
0039-9140
CONICET Digital
CONICET
url http://hdl.handle.net/11336/63933
identifier_str_mv Acebal, Carolina Cecilia; Sklená?ová, Hana; Skrlíková, Jana; Srámková, Ivana; Andruch, Vasil; et al.; Application of DV-SIA manifold for determination of thiocyanate ions in human saliva samples; Elsevier Science; Talanta; 96; 7-2012; 107-112
0039-9140
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.talanta.2012.01.021
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0039914012000276
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613402528317440
score 13.069144

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