Polyphenolic substrates and dyes degradation by yeasts from 25 de Mayo/King George Island (Antarctica)

Autores
Rovati, Jose Ignacio; Pajot, Hipólito Fernando; Ruberto, Lucas Adolfo Mauro; Mac Cormack, Walter Patricio; Castellanos de Figueroa, Lucia Ines
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Antarctica offers a range of extreme climatic conditions, such as low temperatures, high solar radiation and low nutrient availability, and constitutes one of the harshest environments on Earth. Despite that, it has been successfully colonized by 'cold-loving' fungi, which play a key role in decomposition cycles in cold ecosystems. However, knowledge about the ecological role of yeasts in nutrient or organic matter recycling/mineralization remains highly fragmentary. The aim of this work was to study the yeast microbiota in samples collected on 25 de Mayo/King George Island regarding the scope of their ability to degrade polyphenolic substrates such as lignin and azo dyes. Sixty-one yeast isolates were obtained from 37 samples, including soil, rocks, wood and bones. Molecular analyses based on rDNA sequences revealed that 35 yeasts could be identified at the species level and could be classified in the genera Leucosporidiella, Rhodotorula, Cryptococcus, Bullera and Candida. Cryptococcus victoriae was by far the most ubiquitous species. In total, 33% of the yeast isolates examined showed significant activity for dye decolorization, 25% for laccase activity and 38% for ligninolytic activity. Eleven yeasts did not show positive activity in any of the assays performed and no isolates showed positive activity across all tested substrates. A high diversity of yeasts were isolated in this work, possibly including undescribed species and conspicuous Antarctic yeasts, most of them belonging to oligotrophic, slow-growing and metabolically diverse basidiomycetous genera.
Fil: Rovati, Jose Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pajot, Hipólito Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ruberto, Lucas Adolfo Mauro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mac Cormack, Walter Patricio. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: Castellanos de Figueroa, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Materia
ANTARCTIC YEASTS
EXTRACELLULAR ENZYME ACTIVITIES
PSYCHROPHILIC-PSYCHROTOLERANT YEASTS
RDNA YEAST IDENTIFICATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1732
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/1732
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Polyphenolic substrates and dyes degradation by yeasts from 25 de Mayo/King George Island (Antarctica)Rovati, Jose IgnacioPajot, Hipólito FernandoRuberto, Lucas Adolfo MauroMac Cormack, Walter PatricioCastellanos de Figueroa, Lucia InesANTARCTIC YEASTSEXTRACELLULAR ENZYME ACTIVITIESPSYCHROPHILIC-PSYCHROTOLERANT YEASTSRDNA YEAST IDENTIFICATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Antarctica offers a range of extreme climatic conditions, such as low temperatures, high solar radiation and low nutrient availability, and constitutes one of the harshest environments on Earth. Despite that, it has been successfully colonized by 'cold-loving' fungi, which play a key role in decomposition cycles in cold ecosystems. However, knowledge about the ecological role of yeasts in nutrient or organic matter recycling/mineralization remains highly fragmentary. The aim of this work was to study the yeast microbiota in samples collected on 25 de Mayo/King George Island regarding the scope of their ability to degrade polyphenolic substrates such as lignin and azo dyes. Sixty-one yeast isolates were obtained from 37 samples, including soil, rocks, wood and bones. Molecular analyses based on rDNA sequences revealed that 35 yeasts could be identified at the species level and could be classified in the genera Leucosporidiella, Rhodotorula, Cryptococcus, Bullera and Candida. Cryptococcus victoriae was by far the most ubiquitous species. In total, 33% of the yeast isolates examined showed significant activity for dye decolorization, 25% for laccase activity and 38% for ligninolytic activity. Eleven yeasts did not show positive activity in any of the assays performed and no isolates showed positive activity across all tested substrates. A high diversity of yeasts were isolated in this work, possibly including undescribed species and conspicuous Antarctic yeasts, most of them belonging to oligotrophic, slow-growing and metabolically diverse basidiomycetous genera.Fil: Rovati, Jose Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pajot, Hipólito Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Ruberto, Lucas Adolfo Mauro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mac Cormack, Walter Patricio. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; ArgentinaFil: Castellanos de Figueroa, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaJohn Wiley & Sons Ltd2013-10-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1732Rovati, Jose Ignacio; Pajot, Hipólito Fernando; Ruberto, Lucas Adolfo Mauro; Mac Cormack, Walter Patricio; Castellanos de Figueroa, Lucia Ines; Polyphenolic substrates and dyes degradation by yeasts from 25 de Mayo/King George Island (Antarctica); John Wiley & Sons Ltd; Yeast; 30; 11; 7-10-2013; 459-4700749-503Xenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/yea.2982/pdfinfo:eu-repo/semantics/altIdentifier/doi/DOI:10.1002/yea.2982info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:42Zoai:ri.conicet.gov.ar:11336/1732instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:43.197CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Polyphenolic substrates and dyes degradation by yeasts from 25 de Mayo/King George Island (Antarctica)
title Polyphenolic substrates and dyes degradation by yeasts from 25 de Mayo/King George Island (Antarctica)
spellingShingle Polyphenolic substrates and dyes degradation by yeasts from 25 de Mayo/King George Island (Antarctica)
Rovati, Jose Ignacio
ANTARCTIC YEASTS
EXTRACELLULAR ENZYME ACTIVITIES
PSYCHROPHILIC-PSYCHROTOLERANT YEASTS
RDNA YEAST IDENTIFICATION
title_short Polyphenolic substrates and dyes degradation by yeasts from 25 de Mayo/King George Island (Antarctica)
title_full Polyphenolic substrates and dyes degradation by yeasts from 25 de Mayo/King George Island (Antarctica)
title_fullStr Polyphenolic substrates and dyes degradation by yeasts from 25 de Mayo/King George Island (Antarctica)
title_full_unstemmed Polyphenolic substrates and dyes degradation by yeasts from 25 de Mayo/King George Island (Antarctica)
title_sort Polyphenolic substrates and dyes degradation by yeasts from 25 de Mayo/King George Island (Antarctica)
dc.creator.none.fl_str_mv Rovati, Jose Ignacio
Pajot, Hipólito Fernando
Ruberto, Lucas Adolfo Mauro
Mac Cormack, Walter Patricio
Castellanos de Figueroa, Lucia Ines
author Rovati, Jose Ignacio
author_facet Rovati, Jose Ignacio
Pajot, Hipólito Fernando
Ruberto, Lucas Adolfo Mauro
Mac Cormack, Walter Patricio
Castellanos de Figueroa, Lucia Ines
author_role author
author2 Pajot, Hipólito Fernando
Ruberto, Lucas Adolfo Mauro
Mac Cormack, Walter Patricio
Castellanos de Figueroa, Lucia Ines
author2_role author
author
author
author
dc.subject.none.fl_str_mv ANTARCTIC YEASTS
EXTRACELLULAR ENZYME ACTIVITIES
PSYCHROPHILIC-PSYCHROTOLERANT YEASTS
RDNA YEAST IDENTIFICATION
topic ANTARCTIC YEASTS
EXTRACELLULAR ENZYME ACTIVITIES
PSYCHROPHILIC-PSYCHROTOLERANT YEASTS
RDNA YEAST IDENTIFICATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Antarctica offers a range of extreme climatic conditions, such as low temperatures, high solar radiation and low nutrient availability, and constitutes one of the harshest environments on Earth. Despite that, it has been successfully colonized by 'cold-loving' fungi, which play a key role in decomposition cycles in cold ecosystems. However, knowledge about the ecological role of yeasts in nutrient or organic matter recycling/mineralization remains highly fragmentary. The aim of this work was to study the yeast microbiota in samples collected on 25 de Mayo/King George Island regarding the scope of their ability to degrade polyphenolic substrates such as lignin and azo dyes. Sixty-one yeast isolates were obtained from 37 samples, including soil, rocks, wood and bones. Molecular analyses based on rDNA sequences revealed that 35 yeasts could be identified at the species level and could be classified in the genera Leucosporidiella, Rhodotorula, Cryptococcus, Bullera and Candida. Cryptococcus victoriae was by far the most ubiquitous species. In total, 33% of the yeast isolates examined showed significant activity for dye decolorization, 25% for laccase activity and 38% for ligninolytic activity. Eleven yeasts did not show positive activity in any of the assays performed and no isolates showed positive activity across all tested substrates. A high diversity of yeasts were isolated in this work, possibly including undescribed species and conspicuous Antarctic yeasts, most of them belonging to oligotrophic, slow-growing and metabolically diverse basidiomycetous genera.
Fil: Rovati, Jose Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pajot, Hipólito Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ruberto, Lucas Adolfo Mauro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mac Cormack, Walter Patricio. Ministerio de Relaciones Exteriores, Comercio Interno y Culto. Dirección Nacional del Antártico. Instituto Antártico Argentino; Argentina
Fil: Castellanos de Figueroa, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
description Antarctica offers a range of extreme climatic conditions, such as low temperatures, high solar radiation and low nutrient availability, and constitutes one of the harshest environments on Earth. Despite that, it has been successfully colonized by 'cold-loving' fungi, which play a key role in decomposition cycles in cold ecosystems. However, knowledge about the ecological role of yeasts in nutrient or organic matter recycling/mineralization remains highly fragmentary. The aim of this work was to study the yeast microbiota in samples collected on 25 de Mayo/King George Island regarding the scope of their ability to degrade polyphenolic substrates such as lignin and azo dyes. Sixty-one yeast isolates were obtained from 37 samples, including soil, rocks, wood and bones. Molecular analyses based on rDNA sequences revealed that 35 yeasts could be identified at the species level and could be classified in the genera Leucosporidiella, Rhodotorula, Cryptococcus, Bullera and Candida. Cryptococcus victoriae was by far the most ubiquitous species. In total, 33% of the yeast isolates examined showed significant activity for dye decolorization, 25% for laccase activity and 38% for ligninolytic activity. Eleven yeasts did not show positive activity in any of the assays performed and no isolates showed positive activity across all tested substrates. A high diversity of yeasts were isolated in this work, possibly including undescribed species and conspicuous Antarctic yeasts, most of them belonging to oligotrophic, slow-growing and metabolically diverse basidiomycetous genera.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1732
Rovati, Jose Ignacio; Pajot, Hipólito Fernando; Ruberto, Lucas Adolfo Mauro; Mac Cormack, Walter Patricio; Castellanos de Figueroa, Lucia Ines; Polyphenolic substrates and dyes degradation by yeasts from 25 de Mayo/King George Island (Antarctica); John Wiley & Sons Ltd; Yeast; 30; 11; 7-10-2013; 459-470
0749-503X
url http://hdl.handle.net/11336/1732
identifier_str_mv Rovati, Jose Ignacio; Pajot, Hipólito Fernando; Ruberto, Lucas Adolfo Mauro; Mac Cormack, Walter Patricio; Castellanos de Figueroa, Lucia Ines; Polyphenolic substrates and dyes degradation by yeasts from 25 de Mayo/King George Island (Antarctica); John Wiley & Sons Ltd; Yeast; 30; 11; 7-10-2013; 459-470
0749-503X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/yea.2982/pdf
info:eu-repo/semantics/altIdentifier/doi/DOI:10.1002/yea.2982
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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