Identification and susceptibility of clinical isolates of candida spp. To killer toxins

Autores
Robledo Leal, E.; Rivera Morales, L. G.; Sangorrin, Marcela Paula; González, G. M.; Ramos Alfano, G.; Adame Rodriguez, J. M.; Alcocer Gonzalez, J. M.; Arechiga Carvajal, E. T.; Rodriguez Padilla, C.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although invasive infections and mortality caused by Candida species are increasing among compromised patients, resistance to common antifungal agents is also an increasing problem. We analyzed 60 yeasts isolated from patients with invasive candidiasis using a PCR/RFLP strategy based on the internal transcribed spacer (ITS2) region to identify different Candida pathogenic species. PCR analysis was performed from genomic DNA with a primer pair of the ITS2-5.8S rDNA region. PCR-positive samples were characterized by RFLP. Restriction resulted in 23 isolates identified as C. albicans using AlwI, 24 isolates as C. parapsilosis using RsaI, and 13 as C. tropicalis using XmaI. Then, a group of all isolates were evaluated for their susceptibility to a panel of previously described killer yeasts, resulting in 75% being susceptible to at least one killer yeast while the remaining were not inhibited by any strain. C. albicans was the most susceptible group while C. tropicalis had the fewest inhibitions. No species-specific pattern of inhibition was obtained with this panel of killer yeasts. Metschnikowia pulcherrima, Pichia kluyveri and Wickerhamomyces anomalus were the strains that inhibited the most isolates of Candida spp.
Fil: Robledo Leal, E.. Universidad Autónoma de Nuevo León; México
Fil: Rivera Morales, L. G.. Universidad Autónoma de Nuevo León; México
Fil: Sangorrin, Marcela Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue; Argentina
Fil: González, G. M.. Hospital Universitario Dr. Jose Eleuterio Gonzalez; México
Fil: Ramos Alfano, G.. Universidad Autónoma de Nuevo León; México
Fil: Adame Rodriguez, J. M.. Universidad Autónoma de Nuevo León; México
Fil: Alcocer Gonzalez, J. M.. Universidad Autónoma de Nuevo León; México
Fil: Arechiga Carvajal, E. T.. Universidad Autónoma de Nuevo León; México
Fil: Rodriguez Padilla, C.. Universidad Autónoma de Nuevo León; México
Materia
CANDIDA
KILLER YEASTS
YEAST ANTAGONISM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/88929
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/88929
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Identification and susceptibility of clinical isolates of candida spp. To killer toxinsIdentificação e susceptibilidade de isolados clínicos de candida spp. Para toxinas assassinasRobledo Leal, E.Rivera Morales, L. G.Sangorrin, Marcela PaulaGonzález, G. M.Ramos Alfano, G.Adame Rodriguez, J. M.Alcocer Gonzalez, J. M.Arechiga Carvajal, E. T.Rodriguez Padilla, C.CANDIDAKILLER YEASTSYEAST ANTAGONISMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Although invasive infections and mortality caused by Candida species are increasing among compromised patients, resistance to common antifungal agents is also an increasing problem. We analyzed 60 yeasts isolated from patients with invasive candidiasis using a PCR/RFLP strategy based on the internal transcribed spacer (ITS2) region to identify different Candida pathogenic species. PCR analysis was performed from genomic DNA with a primer pair of the ITS2-5.8S rDNA region. PCR-positive samples were characterized by RFLP. Restriction resulted in 23 isolates identified as C. albicans using AlwI, 24 isolates as C. parapsilosis using RsaI, and 13 as C. tropicalis using XmaI. Then, a group of all isolates were evaluated for their susceptibility to a panel of previously described killer yeasts, resulting in 75% being susceptible to at least one killer yeast while the remaining were not inhibited by any strain. C. albicans was the most susceptible group while C. tropicalis had the fewest inhibitions. No species-specific pattern of inhibition was obtained with this panel of killer yeasts. Metschnikowia pulcherrima, Pichia kluyveri and Wickerhamomyces anomalus were the strains that inhibited the most isolates of Candida spp.Fil: Robledo Leal, E.. Universidad Autónoma de Nuevo León; MéxicoFil: Rivera Morales, L. G.. Universidad Autónoma de Nuevo León; MéxicoFil: Sangorrin, Marcela Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue; ArgentinaFil: González, G. M.. Hospital Universitario Dr. Jose Eleuterio Gonzalez; MéxicoFil: Ramos Alfano, G.. Universidad Autónoma de Nuevo León; MéxicoFil: Adame Rodriguez, J. M.. Universidad Autónoma de Nuevo León; MéxicoFil: Alcocer Gonzalez, J. M.. Universidad Autónoma de Nuevo León; MéxicoFil: Arechiga Carvajal, E. T.. Universidad Autónoma de Nuevo León; MéxicoFil: Rodriguez Padilla, C.. Universidad Autónoma de Nuevo León; MéxicoInstituto Internacional de Ecología2018-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/88929Robledo Leal, E.; Rivera Morales, L. G.; Sangorrin, Marcela Paula; González, G. M.; Ramos Alfano, G.; et al.; Identification and susceptibility of clinical isolates of candida spp. To killer toxins ; Instituto Internacional de Ecología; Brazilian Journal of Biology; 78; 4; 11-2018; 742-7491519-69841678-4375CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/c55pw7info:eu-repo/semantics/altIdentifier/doi/10.1590/1519-6984.175635info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:09Zoai:ri.conicet.gov.ar:11336/88929instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:10.194CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification and susceptibility of clinical isolates of candida spp. To killer toxins
Identificação e susceptibilidade de isolados clínicos de candida spp. Para toxinas assassinas
title Identification and susceptibility of clinical isolates of candida spp. To killer toxins
spellingShingle Identification and susceptibility of clinical isolates of candida spp. To killer toxins
Robledo Leal, E.
CANDIDA
KILLER YEASTS
YEAST ANTAGONISM
title_short Identification and susceptibility of clinical isolates of candida spp. To killer toxins
title_full Identification and susceptibility of clinical isolates of candida spp. To killer toxins
title_fullStr Identification and susceptibility of clinical isolates of candida spp. To killer toxins
title_full_unstemmed Identification and susceptibility of clinical isolates of candida spp. To killer toxins
title_sort Identification and susceptibility of clinical isolates of candida spp. To killer toxins
dc.creator.none.fl_str_mv Robledo Leal, E.
Rivera Morales, L. G.
Sangorrin, Marcela Paula
González, G. M.
Ramos Alfano, G.
Adame Rodriguez, J. M.
Alcocer Gonzalez, J. M.
Arechiga Carvajal, E. T.
Rodriguez Padilla, C.
author Robledo Leal, E.
author_facet Robledo Leal, E.
Rivera Morales, L. G.
Sangorrin, Marcela Paula
González, G. M.
Ramos Alfano, G.
Adame Rodriguez, J. M.
Alcocer Gonzalez, J. M.
Arechiga Carvajal, E. T.
Rodriguez Padilla, C.
author_role author
author2 Rivera Morales, L. G.
Sangorrin, Marcela Paula
González, G. M.
Ramos Alfano, G.
Adame Rodriguez, J. M.
Alcocer Gonzalez, J. M.
Arechiga Carvajal, E. T.
Rodriguez Padilla, C.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CANDIDA
KILLER YEASTS
YEAST ANTAGONISM
topic CANDIDA
KILLER YEASTS
YEAST ANTAGONISM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Although invasive infections and mortality caused by Candida species are increasing among compromised patients, resistance to common antifungal agents is also an increasing problem. We analyzed 60 yeasts isolated from patients with invasive candidiasis using a PCR/RFLP strategy based on the internal transcribed spacer (ITS2) region to identify different Candida pathogenic species. PCR analysis was performed from genomic DNA with a primer pair of the ITS2-5.8S rDNA region. PCR-positive samples were characterized by RFLP. Restriction resulted in 23 isolates identified as C. albicans using AlwI, 24 isolates as C. parapsilosis using RsaI, and 13 as C. tropicalis using XmaI. Then, a group of all isolates were evaluated for their susceptibility to a panel of previously described killer yeasts, resulting in 75% being susceptible to at least one killer yeast while the remaining were not inhibited by any strain. C. albicans was the most susceptible group while C. tropicalis had the fewest inhibitions. No species-specific pattern of inhibition was obtained with this panel of killer yeasts. Metschnikowia pulcherrima, Pichia kluyveri and Wickerhamomyces anomalus were the strains that inhibited the most isolates of Candida spp.
Fil: Robledo Leal, E.. Universidad Autónoma de Nuevo León; México
Fil: Rivera Morales, L. G.. Universidad Autónoma de Nuevo León; México
Fil: Sangorrin, Marcela Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue; Argentina
Fil: González, G. M.. Hospital Universitario Dr. Jose Eleuterio Gonzalez; México
Fil: Ramos Alfano, G.. Universidad Autónoma de Nuevo León; México
Fil: Adame Rodriguez, J. M.. Universidad Autónoma de Nuevo León; México
Fil: Alcocer Gonzalez, J. M.. Universidad Autónoma de Nuevo León; México
Fil: Arechiga Carvajal, E. T.. Universidad Autónoma de Nuevo León; México
Fil: Rodriguez Padilla, C.. Universidad Autónoma de Nuevo León; México
description Although invasive infections and mortality caused by Candida species are increasing among compromised patients, resistance to common antifungal agents is also an increasing problem. We analyzed 60 yeasts isolated from patients with invasive candidiasis using a PCR/RFLP strategy based on the internal transcribed spacer (ITS2) region to identify different Candida pathogenic species. PCR analysis was performed from genomic DNA with a primer pair of the ITS2-5.8S rDNA region. PCR-positive samples were characterized by RFLP. Restriction resulted in 23 isolates identified as C. albicans using AlwI, 24 isolates as C. parapsilosis using RsaI, and 13 as C. tropicalis using XmaI. Then, a group of all isolates were evaluated for their susceptibility to a panel of previously described killer yeasts, resulting in 75% being susceptible to at least one killer yeast while the remaining were not inhibited by any strain. C. albicans was the most susceptible group while C. tropicalis had the fewest inhibitions. No species-specific pattern of inhibition was obtained with this panel of killer yeasts. Metschnikowia pulcherrima, Pichia kluyveri and Wickerhamomyces anomalus were the strains that inhibited the most isolates of Candida spp.
publishDate 2018
dc.date.none.fl_str_mv 2018-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/88929
Robledo Leal, E.; Rivera Morales, L. G.; Sangorrin, Marcela Paula; González, G. M.; Ramos Alfano, G.; et al.; Identification and susceptibility of clinical isolates of candida spp. To killer toxins ; Instituto Internacional de Ecología; Brazilian Journal of Biology; 78; 4; 11-2018; 742-749
1519-6984
1678-4375
CONICET Digital
CONICET
url http://hdl.handle.net/11336/88929
identifier_str_mv Robledo Leal, E.; Rivera Morales, L. G.; Sangorrin, Marcela Paula; González, G. M.; Ramos Alfano, G.; et al.; Identification and susceptibility of clinical isolates of candida spp. To killer toxins ; Instituto Internacional de Ecología; Brazilian Journal of Biology; 78; 4; 11-2018; 742-749
1519-6984
1678-4375
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/c55pw7
info:eu-repo/semantics/altIdentifier/doi/10.1590/1519-6984.175635
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Instituto Internacional de Ecología
publisher.none.fl_str_mv Instituto Internacional de Ecología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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