Oral administration of Linoleic acid induces new vessel formation and improves skin wound healing in diabetic rats

Autores
Rodrigues, Hosana G.; Vinolo, Marco A. R.; Sato, Fabio T.; Magdalon, Juliana; Kuhl, Carolina M. C.; Yamagata, Ana S.; Pessoa, Ana Flávia M.; Malheiros, Gabriella; Dos Santos, Marinilce F.; Lima, Camila; Farsky, Sandra H.; Camara, Niels O. S.; Williner, María Rosa; Bernal, Claudio Adrian; Calder, Philip C.; Curi, Rui
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: Impaired wound healing has been widely reported in diabetes. Linoleic acid (LA) accelerates the skin wound healing process in non-diabetic rats. However, LA has not been tested in diabetic animals. Objectives: We investigated whether oral administration of pure LA improves wound healing in streptozotocin- induced diabetic rats. Methods: Dorsal wounds were induced in streptozotocin-induced type-1 diabetic rats treated or not with LA (0.22 g/kg b.w.) for 10 days. Wound closure was daily assessed for two weeks. Wound tissues were collected at specific time-points and used to measure fatty acid composition, and contents of cytokines, growth factors and eicosanoids. Histological and qPCR analyses were employed to examine the dynamics of cell migration during the healing process. Results: LA reduced the wound area 14 days after wound induction. LA also increased the concentrations of cytokine-induced neutrophil chemotaxis (CINC-2αβ), tumor necrosis factor-α (TNF-α) and leukotriene B4 (LTB4), and reduced the expression of macrophage chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1 (MIP-1). These results together with the histological analysis, which showed accumulation of leukocytes in the wound early in the healing process, indicate that LA brought forward the inflammatory phase and improved wound healing in diabetic rats. Angiogenesis was induced by LA through elevation in tissue content of key mediators of this process: vascular-endothelial growth factor (VEGF) and angiopoietin-2 (ANGPT-2).
Fil: Rodrigues, Hosana G.. Universidade Estadual de Campinas; Brasil. Universidade de Sao Paulo; Brasil
Fil: Vinolo, Marco A. R.. Universidade Estadual de Campinas; Brasil
Fil: Sato, Fabio T.. Universidade Estadual de Campinas; Brasil
Fil: Magdalon, Juliana. Universidade de Sao Paulo; Brasil
Fil: Kuhl, Carolina M. C.. Universidade Estadual de Campinas; Brasil
Fil: Yamagata, Ana S.. Universidade de Sao Paulo; Brasil
Fil: Pessoa, Ana Flávia M.. Universidade de Sao Paulo; Brasil
Fil: Malheiros, Gabriella. Universidade de Sao Paulo; Brasil
Fil: Dos Santos, Marinilce F.. Universidade de Sao Paulo; Brasil
Fil: Lima, Camila. Universidade de Sao Paulo; Brasil
Fil: Farsky, Sandra H.. Universidade de Sao Paulo; Brasil
Fil: Camara, Niels O. S.. Universidade de Sao Paulo; Brasil
Fil: Williner, María Rosa. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Bernal, Claudio Adrian. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Calder, Philip C.. University of Southampton; Reino Unido
Fil: Curi, Rui. Universidade de Sao Paulo; Brasil
Materia
Oral Administration of Linoleic Acid
Skin Wound Healing
Diabetic Rats
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/71429
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/71429
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Oral administration of Linoleic acid induces new vessel formation and improves skin wound healing in diabetic ratsRodrigues, Hosana G.Vinolo, Marco A. R.Sato, Fabio T.Magdalon, JulianaKuhl, Carolina M. C.Yamagata, Ana S.Pessoa, Ana Flávia M.Malheiros, GabriellaDos Santos, Marinilce F.Lima, CamilaFarsky, Sandra H.Camara, Niels O. S.Williner, María RosaBernal, Claudio AdrianCalder, Philip C.Curi, RuiOral Administration of Linoleic AcidSkin Wound HealingDiabetic Ratshttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Introduction: Impaired wound healing has been widely reported in diabetes. Linoleic acid (LA) accelerates the skin wound healing process in non-diabetic rats. However, LA has not been tested in diabetic animals. Objectives: We investigated whether oral administration of pure LA improves wound healing in streptozotocin- induced diabetic rats. Methods: Dorsal wounds were induced in streptozotocin-induced type-1 diabetic rats treated or not with LA (0.22 g/kg b.w.) for 10 days. Wound closure was daily assessed for two weeks. Wound tissues were collected at specific time-points and used to measure fatty acid composition, and contents of cytokines, growth factors and eicosanoids. Histological and qPCR analyses were employed to examine the dynamics of cell migration during the healing process. Results: LA reduced the wound area 14 days after wound induction. LA also increased the concentrations of cytokine-induced neutrophil chemotaxis (CINC-2αβ), tumor necrosis factor-α (TNF-α) and leukotriene B4 (LTB4), and reduced the expression of macrophage chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1 (MIP-1). These results together with the histological analysis, which showed accumulation of leukocytes in the wound early in the healing process, indicate that LA brought forward the inflammatory phase and improved wound healing in diabetic rats. Angiogenesis was induced by LA through elevation in tissue content of key mediators of this process: vascular-endothelial growth factor (VEGF) and angiopoietin-2 (ANGPT-2).Fil: Rodrigues, Hosana G.. Universidade Estadual de Campinas; Brasil. Universidade de Sao Paulo; BrasilFil: Vinolo, Marco A. R.. Universidade Estadual de Campinas; BrasilFil: Sato, Fabio T.. Universidade Estadual de Campinas; BrasilFil: Magdalon, Juliana. Universidade de Sao Paulo; BrasilFil: Kuhl, Carolina M. C.. Universidade Estadual de Campinas; BrasilFil: Yamagata, Ana S.. Universidade de Sao Paulo; BrasilFil: Pessoa, Ana Flávia M.. Universidade de Sao Paulo; BrasilFil: Malheiros, Gabriella. Universidade de Sao Paulo; BrasilFil: Dos Santos, Marinilce F.. Universidade de Sao Paulo; BrasilFil: Lima, Camila. Universidade de Sao Paulo; BrasilFil: Farsky, Sandra H.. Universidade de Sao Paulo; BrasilFil: Camara, Niels O. S.. Universidade de Sao Paulo; BrasilFil: Williner, María Rosa. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Bernal, Claudio Adrian. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Calder, Philip C.. University of Southampton; Reino UnidoFil: Curi, Rui. Universidade de Sao Paulo; BrasilPublic Library of Science2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71429Rodrigues, Hosana G.; Vinolo, Marco A. R.; Sato, Fabio T.; Magdalon, Juliana; Kuhl, Carolina M. C.; et al.; Oral administration of Linoleic acid induces new vessel formation and improves skin wound healing in diabetic rats; Public Library of Science; Plos One; 11; 10; 10-2016; 1-19; e01651151932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0165115info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0165115info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:03Zoai:ri.conicet.gov.ar:11336/71429instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:03.495CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Oral administration of Linoleic acid induces new vessel formation and improves skin wound healing in diabetic rats
title Oral administration of Linoleic acid induces new vessel formation and improves skin wound healing in diabetic rats
spellingShingle Oral administration of Linoleic acid induces new vessel formation and improves skin wound healing in diabetic rats
Rodrigues, Hosana G.
Oral Administration of Linoleic Acid
Skin Wound Healing
Diabetic Rats
title_short Oral administration of Linoleic acid induces new vessel formation and improves skin wound healing in diabetic rats
title_full Oral administration of Linoleic acid induces new vessel formation and improves skin wound healing in diabetic rats
title_fullStr Oral administration of Linoleic acid induces new vessel formation and improves skin wound healing in diabetic rats
title_full_unstemmed Oral administration of Linoleic acid induces new vessel formation and improves skin wound healing in diabetic rats
title_sort Oral administration of Linoleic acid induces new vessel formation and improves skin wound healing in diabetic rats
dc.creator.none.fl_str_mv Rodrigues, Hosana G.
Vinolo, Marco A. R.
Sato, Fabio T.
Magdalon, Juliana
Kuhl, Carolina M. C.
Yamagata, Ana S.
Pessoa, Ana Flávia M.
Malheiros, Gabriella
Dos Santos, Marinilce F.
Lima, Camila
Farsky, Sandra H.
Camara, Niels O. S.
Williner, María Rosa
Bernal, Claudio Adrian
Calder, Philip C.
Curi, Rui
author Rodrigues, Hosana G.
author_facet Rodrigues, Hosana G.
Vinolo, Marco A. R.
Sato, Fabio T.
Magdalon, Juliana
Kuhl, Carolina M. C.
Yamagata, Ana S.
Pessoa, Ana Flávia M.
Malheiros, Gabriella
Dos Santos, Marinilce F.
Lima, Camila
Farsky, Sandra H.
Camara, Niels O. S.
Williner, María Rosa
Bernal, Claudio Adrian
Calder, Philip C.
Curi, Rui
author_role author
author2 Vinolo, Marco A. R.
Sato, Fabio T.
Magdalon, Juliana
Kuhl, Carolina M. C.
Yamagata, Ana S.
Pessoa, Ana Flávia M.
Malheiros, Gabriella
Dos Santos, Marinilce F.
Lima, Camila
Farsky, Sandra H.
Camara, Niels O. S.
Williner, María Rosa
Bernal, Claudio Adrian
Calder, Philip C.
Curi, Rui
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Oral Administration of Linoleic Acid
Skin Wound Healing
Diabetic Rats
topic Oral Administration of Linoleic Acid
Skin Wound Healing
Diabetic Rats
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Introduction: Impaired wound healing has been widely reported in diabetes. Linoleic acid (LA) accelerates the skin wound healing process in non-diabetic rats. However, LA has not been tested in diabetic animals. Objectives: We investigated whether oral administration of pure LA improves wound healing in streptozotocin- induced diabetic rats. Methods: Dorsal wounds were induced in streptozotocin-induced type-1 diabetic rats treated or not with LA (0.22 g/kg b.w.) for 10 days. Wound closure was daily assessed for two weeks. Wound tissues were collected at specific time-points and used to measure fatty acid composition, and contents of cytokines, growth factors and eicosanoids. Histological and qPCR analyses were employed to examine the dynamics of cell migration during the healing process. Results: LA reduced the wound area 14 days after wound induction. LA also increased the concentrations of cytokine-induced neutrophil chemotaxis (CINC-2αβ), tumor necrosis factor-α (TNF-α) and leukotriene B4 (LTB4), and reduced the expression of macrophage chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1 (MIP-1). These results together with the histological analysis, which showed accumulation of leukocytes in the wound early in the healing process, indicate that LA brought forward the inflammatory phase and improved wound healing in diabetic rats. Angiogenesis was induced by LA through elevation in tissue content of key mediators of this process: vascular-endothelial growth factor (VEGF) and angiopoietin-2 (ANGPT-2).
Fil: Rodrigues, Hosana G.. Universidade Estadual de Campinas; Brasil. Universidade de Sao Paulo; Brasil
Fil: Vinolo, Marco A. R.. Universidade Estadual de Campinas; Brasil
Fil: Sato, Fabio T.. Universidade Estadual de Campinas; Brasil
Fil: Magdalon, Juliana. Universidade de Sao Paulo; Brasil
Fil: Kuhl, Carolina M. C.. Universidade Estadual de Campinas; Brasil
Fil: Yamagata, Ana S.. Universidade de Sao Paulo; Brasil
Fil: Pessoa, Ana Flávia M.. Universidade de Sao Paulo; Brasil
Fil: Malheiros, Gabriella. Universidade de Sao Paulo; Brasil
Fil: Dos Santos, Marinilce F.. Universidade de Sao Paulo; Brasil
Fil: Lima, Camila. Universidade de Sao Paulo; Brasil
Fil: Farsky, Sandra H.. Universidade de Sao Paulo; Brasil
Fil: Camara, Niels O. S.. Universidade de Sao Paulo; Brasil
Fil: Williner, María Rosa. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Bernal, Claudio Adrian. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Calder, Philip C.. University of Southampton; Reino Unido
Fil: Curi, Rui. Universidade de Sao Paulo; Brasil
description Introduction: Impaired wound healing has been widely reported in diabetes. Linoleic acid (LA) accelerates the skin wound healing process in non-diabetic rats. However, LA has not been tested in diabetic animals. Objectives: We investigated whether oral administration of pure LA improves wound healing in streptozotocin- induced diabetic rats. Methods: Dorsal wounds were induced in streptozotocin-induced type-1 diabetic rats treated or not with LA (0.22 g/kg b.w.) for 10 days. Wound closure was daily assessed for two weeks. Wound tissues were collected at specific time-points and used to measure fatty acid composition, and contents of cytokines, growth factors and eicosanoids. Histological and qPCR analyses were employed to examine the dynamics of cell migration during the healing process. Results: LA reduced the wound area 14 days after wound induction. LA also increased the concentrations of cytokine-induced neutrophil chemotaxis (CINC-2αβ), tumor necrosis factor-α (TNF-α) and leukotriene B4 (LTB4), and reduced the expression of macrophage chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1 (MIP-1). These results together with the histological analysis, which showed accumulation of leukocytes in the wound early in the healing process, indicate that LA brought forward the inflammatory phase and improved wound healing in diabetic rats. Angiogenesis was induced by LA through elevation in tissue content of key mediators of this process: vascular-endothelial growth factor (VEGF) and angiopoietin-2 (ANGPT-2).
publishDate 2016
dc.date.none.fl_str_mv 2016-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71429
Rodrigues, Hosana G.; Vinolo, Marco A. R.; Sato, Fabio T.; Magdalon, Juliana; Kuhl, Carolina M. C.; et al.; Oral administration of Linoleic acid induces new vessel formation and improves skin wound healing in diabetic rats; Public Library of Science; Plos One; 11; 10; 10-2016; 1-19; e0165115
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71429
identifier_str_mv Rodrigues, Hosana G.; Vinolo, Marco A. R.; Sato, Fabio T.; Magdalon, Juliana; Kuhl, Carolina M. C.; et al.; Oral administration of Linoleic acid induces new vessel formation and improves skin wound healing in diabetic rats; Public Library of Science; Plos One; 11; 10; 10-2016; 1-19; e0165115
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0165115
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0165115
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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