VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display

Autores
Peralta, Andrea Verónica; Maroniche, Guillermo Andrés; Alfonso, Victoria; Molinari, Maria Paula; Taboga, Oscar Alberto
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Foot-and-mouth disease virus (FMDV) causes important economical losses in livestock farming. In order to develop a novel subunit vaccine against FMDV, we constructed recombinant baculoviruses that display the protein VP1 of FMDV on their surface, with either polar (fused to gp64) or nonpolar (fused to anchor membrane from VSV-G protein) distribution. Insect cells infected with the different recombinant baculoviruses expressed VP1 fusion protein to high levels. However, the recombinant VP1 protein was not carried by budded virions. Subcellular localization of VP1 revealed that the trafficking of the fusion protein to the cell plasma membrane was impaired. Our results suggest that VP1 contains cryptic domains that interfere with protein secretion and subsequent incorporation into budded baculoviruses.
Fil: Peralta, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alfonso, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Molinari, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Taboga, Oscar Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Materia
Baculovirus Display
Display
Biotecnología
Vp1
Foot-And-Mouth Disease Virus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24899
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface displayPeralta, Andrea VerónicaMaroniche, Guillermo AndrésAlfonso, VictoriaMolinari, Maria PaulaTaboga, Oscar AlbertoBaculovirus DisplayDisplayBiotecnologíaVp1Foot-And-Mouth Disease Virushttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The Foot-and-mouth disease virus (FMDV) causes important economical losses in livestock farming. In order to develop a novel subunit vaccine against FMDV, we constructed recombinant baculoviruses that display the protein VP1 of FMDV on their surface, with either polar (fused to gp64) or nonpolar (fused to anchor membrane from VSV-G protein) distribution. Insect cells infected with the different recombinant baculoviruses expressed VP1 fusion protein to high levels. However, the recombinant VP1 protein was not carried by budded virions. Subcellular localization of VP1 revealed that the trafficking of the fusion protein to the cell plasma membrane was impaired. Our results suggest that VP1 contains cryptic domains that interfere with protein secretion and subsequent incorporation into budded baculoviruses.Fil: Peralta, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alfonso, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Molinari, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Taboga, Oscar Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaElsevier2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24899Peralta, Andrea Verónica; Maroniche, Guillermo Andrés; Alfonso, Victoria; Molinari, Maria Paula; Taboga, Oscar Alberto; VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display; Elsevier; Virus Research; 175; 1; 4-2013; 87-900168-1702CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0168170213001123info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virusres.2013.03.018info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:08:07Zoai:ri.conicet.gov.ar:11336/24899instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:08:07.785CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display
title VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display
spellingShingle VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display
Peralta, Andrea Verónica
Baculovirus Display
Display
Biotecnología
Vp1
Foot-And-Mouth Disease Virus
title_short VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display
title_full VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display
title_fullStr VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display
title_full_unstemmed VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display
title_sort VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display
dc.creator.none.fl_str_mv Peralta, Andrea Verónica
Maroniche, Guillermo Andrés
Alfonso, Victoria
Molinari, Maria Paula
Taboga, Oscar Alberto
author Peralta, Andrea Verónica
author_facet Peralta, Andrea Verónica
Maroniche, Guillermo Andrés
Alfonso, Victoria
Molinari, Maria Paula
Taboga, Oscar Alberto
author_role author
author2 Maroniche, Guillermo Andrés
Alfonso, Victoria
Molinari, Maria Paula
Taboga, Oscar Alberto
author2_role author
author
author
author
dc.subject.none.fl_str_mv Baculovirus Display
Display
Biotecnología
Vp1
Foot-And-Mouth Disease Virus
topic Baculovirus Display
Display
Biotecnología
Vp1
Foot-And-Mouth Disease Virus
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The Foot-and-mouth disease virus (FMDV) causes important economical losses in livestock farming. In order to develop a novel subunit vaccine against FMDV, we constructed recombinant baculoviruses that display the protein VP1 of FMDV on their surface, with either polar (fused to gp64) or nonpolar (fused to anchor membrane from VSV-G protein) distribution. Insect cells infected with the different recombinant baculoviruses expressed VP1 fusion protein to high levels. However, the recombinant VP1 protein was not carried by budded virions. Subcellular localization of VP1 revealed that the trafficking of the fusion protein to the cell plasma membrane was impaired. Our results suggest that VP1 contains cryptic domains that interfere with protein secretion and subsequent incorporation into budded baculoviruses.
Fil: Peralta, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alfonso, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Molinari, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Taboga, Oscar Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
description The Foot-and-mouth disease virus (FMDV) causes important economical losses in livestock farming. In order to develop a novel subunit vaccine against FMDV, we constructed recombinant baculoviruses that display the protein VP1 of FMDV on their surface, with either polar (fused to gp64) or nonpolar (fused to anchor membrane from VSV-G protein) distribution. Insect cells infected with the different recombinant baculoviruses expressed VP1 fusion protein to high levels. However, the recombinant VP1 protein was not carried by budded virions. Subcellular localization of VP1 revealed that the trafficking of the fusion protein to the cell plasma membrane was impaired. Our results suggest that VP1 contains cryptic domains that interfere with protein secretion and subsequent incorporation into budded baculoviruses.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24899
Peralta, Andrea Verónica; Maroniche, Guillermo Andrés; Alfonso, Victoria; Molinari, Maria Paula; Taboga, Oscar Alberto; VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display; Elsevier; Virus Research; 175; 1; 4-2013; 87-90
0168-1702
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24899
identifier_str_mv Peralta, Andrea Verónica; Maroniche, Guillermo Andrés; Alfonso, Victoria; Molinari, Maria Paula; Taboga, Oscar Alberto; VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display; Elsevier; Virus Research; 175; 1; 4-2013; 87-90
0168-1702
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0168170213001123
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virusres.2013.03.018
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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