Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins
- Autores
- Rodriguez Furlán, Laura Teresa; Lecot, Carlos Javier; Pérez Padilla, Antonio; Campderrós, Mercedes Edith; Zaritzky, Noemi Elisabet
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- parte de libro
- Estado
- versión publicada
- Descripción
- Inulin is a generic term applied to heterogeneous blends of fructo-oligosaccharides which are reserve carbohydrate sources present in many plant foods such as bananas,onions, garlic, leeks, artichokes and chicory, which represents the main commercial source.This polysaccharide has a wide range of both, nutritional and technological applications.Nutritionally, inulin is regarded as a soluble fiber which promotes the growth of intestinalbacteria, acting as a prebiotic. Also, is a non-digestible carbohydrate with minimal impact on blood sugar and unlike fructose, it is not insulemic and does not raise triglycerides being generally considered suitable for diabetics and potentially helpful in managing blood sugarrelated illnesses . Among the technological benefits, inulin is used as fat and sugar replacement, low caloric bulking agent, texturing and water-binding agent . One general property of the saccharides is the stabilization of proteins by their incorporation into carbohydrate solutions before freeze-drying being this a known preservation procedure. The previous incorporation of saccharide promotes the formation of amorphous, glassy systems, inhibits crystallization and influences the kinetics of deteriorative reactions upon storage by which its structured integrity is maintained . To act successfully as a protectant, the saccharides should have a high glass transition temperature (Tg), a poor hygroscopicity, a low crystallization rate, containing no reducing groups. When freezedrying is envisaged as a method of drying, a relatively high T´g of the freeze concentrated fraction is preferable. Previous studies demonstrated that inulin meets these requirements being excellent protector of therapeutical proteins and viruses over the drying and storage processes .The glass transition temperatures of the maximally concentrated frozen solutions (T´g) wereanalyzed and compared to the experimental results by applying the predictive equations of Miller/Fox and Gordon/Taylor extended for multi-component systems. The glass transition (Tg) of the freeze dried multi-component mixtures, the onset crystallization temperature (Tc) of the solute at temperatures above Tg, in the freeze dried samples were determined.Furthermore, the kinetics of the denaturation and the thermal denaturation (Td) of the freeze-dried samples, at different DSC scan rates, protein concentrations and pH, were analyzed and the thermodynamic compatibility of the different matrix components were determined. The enthalpy change involved in the denaturation reactions of proteins was also determined. A kinetic model that describes bovine plasma proteins denaturation was proposed.
Fil: Rodriguez Furlán, Laura Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Lecot, Carlos Javier. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Pérez Padilla, Antonio. No especifíca;
Fil: Campderrós, Mercedes Edith. No especifíca;
Fil: Zaritzky, Noemi Elisabet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina - Materia
-
Inulin
Cryoprotector
Lyoprotector
Bovine plasma proteins - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/148456
Ver los metadatos del registro completo
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Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma ProteinsRodriguez Furlán, Laura TeresaLecot, Carlos JavierPérez Padilla, AntonioCampderrós, Mercedes EdithZaritzky, Noemi ElisabetInulinCryoprotectorLyoprotectorBovine plasma proteinshttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2Inulin is a generic term applied to heterogeneous blends of fructo-oligosaccharides which are reserve carbohydrate sources present in many plant foods such as bananas,onions, garlic, leeks, artichokes and chicory, which represents the main commercial source.This polysaccharide has a wide range of both, nutritional and technological applications.Nutritionally, inulin is regarded as a soluble fiber which promotes the growth of intestinalbacteria, acting as a prebiotic. Also, is a non-digestible carbohydrate with minimal impact on blood sugar and unlike fructose, it is not insulemic and does not raise triglycerides being generally considered suitable for diabetics and potentially helpful in managing blood sugarrelated illnesses . Among the technological benefits, inulin is used as fat and sugar replacement, low caloric bulking agent, texturing and water-binding agent . One general property of the saccharides is the stabilization of proteins by their incorporation into carbohydrate solutions before freeze-drying being this a known preservation procedure. The previous incorporation of saccharide promotes the formation of amorphous, glassy systems, inhibits crystallization and influences the kinetics of deteriorative reactions upon storage by which its structured integrity is maintained . To act successfully as a protectant, the saccharides should have a high glass transition temperature (Tg), a poor hygroscopicity, a low crystallization rate, containing no reducing groups. When freezedrying is envisaged as a method of drying, a relatively high T´g of the freeze concentrated fraction is preferable. Previous studies demonstrated that inulin meets these requirements being excellent protector of therapeutical proteins and viruses over the drying and storage processes .The glass transition temperatures of the maximally concentrated frozen solutions (T´g) wereanalyzed and compared to the experimental results by applying the predictive equations of Miller/Fox and Gordon/Taylor extended for multi-component systems. The glass transition (Tg) of the freeze dried multi-component mixtures, the onset crystallization temperature (Tc) of the solute at temperatures above Tg, in the freeze dried samples were determined.Furthermore, the kinetics of the denaturation and the thermal denaturation (Td) of the freeze-dried samples, at different DSC scan rates, protein concentrations and pH, were analyzed and the thermodynamic compatibility of the different matrix components were determined. The enthalpy change involved in the denaturation reactions of proteins was also determined. A kinetic model that describes bovine plasma proteins denaturation was proposed.Fil: Rodriguez Furlán, Laura Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: Lecot, Carlos Javier. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Pérez Padilla, Antonio. No especifíca;Fil: Campderrós, Mercedes Edith. No especifíca;Fil: Zaritzky, Noemi Elisabet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaIntechOpenElkordy, Amal Ali2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookParthttp://purl.org/coar/resource_type/c_3248info:ar-repo/semantics/parteDeLibroapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/148456Rodriguez Furlán, Laura Teresa; Lecot, Carlos Javier; Pérez Padilla, Antonio; Campderrós, Mercedes Edith; Zaritzky, Noemi Elisabet; Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins; IntechOpen; 2013; 197-218978-953-51-0947-1CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.intechopen.com/chapters/42266info:eu-repo/semantics/altIdentifier/doi/10.5772/54462info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:33Zoai:ri.conicet.gov.ar:11336/148456instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:33.942CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins |
title |
Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins |
spellingShingle |
Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins Rodriguez Furlán, Laura Teresa Inulin Cryoprotector Lyoprotector Bovine plasma proteins |
title_short |
Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins |
title_full |
Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins |
title_fullStr |
Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins |
title_full_unstemmed |
Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins |
title_sort |
Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins |
dc.creator.none.fl_str_mv |
Rodriguez Furlán, Laura Teresa Lecot, Carlos Javier Pérez Padilla, Antonio Campderrós, Mercedes Edith Zaritzky, Noemi Elisabet |
author |
Rodriguez Furlán, Laura Teresa |
author_facet |
Rodriguez Furlán, Laura Teresa Lecot, Carlos Javier Pérez Padilla, Antonio Campderrós, Mercedes Edith Zaritzky, Noemi Elisabet |
author_role |
author |
author2 |
Lecot, Carlos Javier Pérez Padilla, Antonio Campderrós, Mercedes Edith Zaritzky, Noemi Elisabet |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Elkordy, Amal Ali |
dc.subject.none.fl_str_mv |
Inulin Cryoprotector Lyoprotector Bovine plasma proteins |
topic |
Inulin Cryoprotector Lyoprotector Bovine plasma proteins |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.4 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Inulin is a generic term applied to heterogeneous blends of fructo-oligosaccharides which are reserve carbohydrate sources present in many plant foods such as bananas,onions, garlic, leeks, artichokes and chicory, which represents the main commercial source.This polysaccharide has a wide range of both, nutritional and technological applications.Nutritionally, inulin is regarded as a soluble fiber which promotes the growth of intestinalbacteria, acting as a prebiotic. Also, is a non-digestible carbohydrate with minimal impact on blood sugar and unlike fructose, it is not insulemic and does not raise triglycerides being generally considered suitable for diabetics and potentially helpful in managing blood sugarrelated illnesses . Among the technological benefits, inulin is used as fat and sugar replacement, low caloric bulking agent, texturing and water-binding agent . One general property of the saccharides is the stabilization of proteins by their incorporation into carbohydrate solutions before freeze-drying being this a known preservation procedure. The previous incorporation of saccharide promotes the formation of amorphous, glassy systems, inhibits crystallization and influences the kinetics of deteriorative reactions upon storage by which its structured integrity is maintained . To act successfully as a protectant, the saccharides should have a high glass transition temperature (Tg), a poor hygroscopicity, a low crystallization rate, containing no reducing groups. When freezedrying is envisaged as a method of drying, a relatively high T´g of the freeze concentrated fraction is preferable. Previous studies demonstrated that inulin meets these requirements being excellent protector of therapeutical proteins and viruses over the drying and storage processes .The glass transition temperatures of the maximally concentrated frozen solutions (T´g) wereanalyzed and compared to the experimental results by applying the predictive equations of Miller/Fox and Gordon/Taylor extended for multi-component systems. The glass transition (Tg) of the freeze dried multi-component mixtures, the onset crystallization temperature (Tc) of the solute at temperatures above Tg, in the freeze dried samples were determined.Furthermore, the kinetics of the denaturation and the thermal denaturation (Td) of the freeze-dried samples, at different DSC scan rates, protein concentrations and pH, were analyzed and the thermodynamic compatibility of the different matrix components were determined. The enthalpy change involved in the denaturation reactions of proteins was also determined. A kinetic model that describes bovine plasma proteins denaturation was proposed. Fil: Rodriguez Furlán, Laura Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina Fil: Lecot, Carlos Javier. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina Fil: Pérez Padilla, Antonio. No especifíca; Fil: Campderrós, Mercedes Edith. No especifíca; Fil: Zaritzky, Noemi Elisabet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina |
description |
Inulin is a generic term applied to heterogeneous blends of fructo-oligosaccharides which are reserve carbohydrate sources present in many plant foods such as bananas,onions, garlic, leeks, artichokes and chicory, which represents the main commercial source.This polysaccharide has a wide range of both, nutritional and technological applications.Nutritionally, inulin is regarded as a soluble fiber which promotes the growth of intestinalbacteria, acting as a prebiotic. Also, is a non-digestible carbohydrate with minimal impact on blood sugar and unlike fructose, it is not insulemic and does not raise triglycerides being generally considered suitable for diabetics and potentially helpful in managing blood sugarrelated illnesses . Among the technological benefits, inulin is used as fat and sugar replacement, low caloric bulking agent, texturing and water-binding agent . One general property of the saccharides is the stabilization of proteins by their incorporation into carbohydrate solutions before freeze-drying being this a known preservation procedure. The previous incorporation of saccharide promotes the formation of amorphous, glassy systems, inhibits crystallization and influences the kinetics of deteriorative reactions upon storage by which its structured integrity is maintained . To act successfully as a protectant, the saccharides should have a high glass transition temperature (Tg), a poor hygroscopicity, a low crystallization rate, containing no reducing groups. When freezedrying is envisaged as a method of drying, a relatively high T´g of the freeze concentrated fraction is preferable. Previous studies demonstrated that inulin meets these requirements being excellent protector of therapeutical proteins and viruses over the drying and storage processes .The glass transition temperatures of the maximally concentrated frozen solutions (T´g) wereanalyzed and compared to the experimental results by applying the predictive equations of Miller/Fox and Gordon/Taylor extended for multi-component systems. The glass transition (Tg) of the freeze dried multi-component mixtures, the onset crystallization temperature (Tc) of the solute at temperatures above Tg, in the freeze dried samples were determined.Furthermore, the kinetics of the denaturation and the thermal denaturation (Td) of the freeze-dried samples, at different DSC scan rates, protein concentrations and pH, were analyzed and the thermodynamic compatibility of the different matrix components were determined. The enthalpy change involved in the denaturation reactions of proteins was also determined. A kinetic model that describes bovine plasma proteins denaturation was proposed. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/bookPart http://purl.org/coar/resource_type/c_3248 info:ar-repo/semantics/parteDeLibro |
status_str |
publishedVersion |
format |
bookPart |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/148456 Rodriguez Furlán, Laura Teresa; Lecot, Carlos Javier; Pérez Padilla, Antonio; Campderrós, Mercedes Edith; Zaritzky, Noemi Elisabet; Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins; IntechOpen; 2013; 197-218 978-953-51-0947-1 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/148456 |
identifier_str_mv |
Rodriguez Furlán, Laura Teresa; Lecot, Carlos Javier; Pérez Padilla, Antonio; Campderrós, Mercedes Edith; Zaritzky, Noemi Elisabet; Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins; IntechOpen; 2013; 197-218 978-953-51-0947-1 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.intechopen.com/chapters/42266 info:eu-repo/semantics/altIdentifier/doi/10.5772/54462 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
IntechOpen |
publisher.none.fl_str_mv |
IntechOpen |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |