Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins

Autores
Rodriguez Furlán, Laura Teresa; Lecot, Carlos Javier; Pérez Padilla, Antonio; Campderrós, Mercedes Edith; Zaritzky, Noemi Elisabet
Año de publicación
2013
Idioma
inglés
Tipo de recurso
parte de libro
Estado
versión publicada
Descripción
Inulin is a generic term applied to heterogeneous blends of fructo-oligosaccharides which are reserve carbohydrate sources present in many plant foods such as bananas,onions, garlic, leeks, artichokes and chicory, which represents the main commercial source.This polysaccharide has a wide range of both, nutritional and technological applications.Nutritionally, inulin is regarded as a soluble fiber which promotes the growth of intestinalbacteria, acting as a prebiotic. Also, is a non-digestible carbohydrate with minimal impact on blood sugar and unlike fructose, it is not insulemic and does not raise triglycerides being generally considered suitable for diabetics and potentially helpful in managing blood sugarrelated illnesses . Among the technological benefits, inulin is used as fat and sugar replacement, low caloric bulking agent, texturing and water-binding agent . One general property of the saccharides is the stabilization of proteins by their incorporation into carbohydrate solutions before freeze-drying being this a known preservation procedure. The previous incorporation of saccharide promotes the formation of amorphous, glassy systems, inhibits crystallization and influences the kinetics of deteriorative reactions upon storage by which its structured integrity is maintained . To act successfully as a protectant, the saccharides should have a high glass transition temperature (Tg), a poor hygroscopicity, a low crystallization rate, containing no reducing groups. When freezedrying is envisaged as a method of drying, a relatively high T´g of the freeze concentrated fraction is preferable. Previous studies demonstrated that inulin meets these requirements being excellent protector of therapeutical proteins and viruses over the drying and storage processes .The glass transition temperatures of the maximally concentrated frozen solutions (T´g) wereanalyzed and compared to the experimental results by applying the predictive equations of Miller/Fox and Gordon/Taylor extended for multi-component systems. The glass transition (Tg) of the freeze dried multi-component mixtures, the onset crystallization temperature (Tc) of the solute at temperatures above Tg, in the freeze dried samples were determined.Furthermore, the kinetics of the denaturation and the thermal denaturation (Td) of the freeze-dried samples, at different DSC scan rates, protein concentrations and pH, were analyzed and the thermodynamic compatibility of the different matrix components were determined. The enthalpy change involved in the denaturation reactions of proteins was also determined. A kinetic model that describes bovine plasma proteins denaturation was proposed.
Fil: Rodriguez Furlán, Laura Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Lecot, Carlos Javier. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Pérez Padilla, Antonio. No especifíca;
Fil: Campderrós, Mercedes Edith. No especifíca;
Fil: Zaritzky, Noemi Elisabet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Materia
Inulin
Cryoprotector
Lyoprotector
Bovine plasma proteins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/148456

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oai_identifier_str oai:ri.conicet.gov.ar:11336/148456
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma ProteinsRodriguez Furlán, Laura TeresaLecot, Carlos JavierPérez Padilla, AntonioCampderrós, Mercedes EdithZaritzky, Noemi ElisabetInulinCryoprotectorLyoprotectorBovine plasma proteinshttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2Inulin is a generic term applied to heterogeneous blends of fructo-oligosaccharides which are reserve carbohydrate sources present in many plant foods such as bananas,onions, garlic, leeks, artichokes and chicory, which represents the main commercial source.This polysaccharide has a wide range of both, nutritional and technological applications.Nutritionally, inulin is regarded as a soluble fiber which promotes the growth of intestinalbacteria, acting as a prebiotic. Also, is a non-digestible carbohydrate with minimal impact on blood sugar and unlike fructose, it is not insulemic and does not raise triglycerides being generally considered suitable for diabetics and potentially helpful in managing blood sugarrelated illnesses . Among the technological benefits, inulin is used as fat and sugar replacement, low caloric bulking agent, texturing and water-binding agent . One general property of the saccharides is the stabilization of proteins by their incorporation into carbohydrate solutions before freeze-drying being this a known preservation procedure. The previous incorporation of saccharide promotes the formation of amorphous, glassy systems, inhibits crystallization and influences the kinetics of deteriorative reactions upon storage by which its structured integrity is maintained . To act successfully as a protectant, the saccharides should have a high glass transition temperature (Tg), a poor hygroscopicity, a low crystallization rate, containing no reducing groups. When freezedrying is envisaged as a method of drying, a relatively high T´g of the freeze concentrated fraction is preferable. Previous studies demonstrated that inulin meets these requirements being excellent protector of therapeutical proteins and viruses over the drying and storage processes .The glass transition temperatures of the maximally concentrated frozen solutions (T´g) wereanalyzed and compared to the experimental results by applying the predictive equations of Miller/Fox and Gordon/Taylor extended for multi-component systems. The glass transition (Tg) of the freeze dried multi-component mixtures, the onset crystallization temperature (Tc) of the solute at temperatures above Tg, in the freeze dried samples were determined.Furthermore, the kinetics of the denaturation and the thermal denaturation (Td) of the freeze-dried samples, at different DSC scan rates, protein concentrations and pH, were analyzed and the thermodynamic compatibility of the different matrix components were determined. The enthalpy change involved in the denaturation reactions of proteins was also determined. A kinetic model that describes bovine plasma proteins denaturation was proposed.Fil: Rodriguez Furlán, Laura Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: Lecot, Carlos Javier. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Pérez Padilla, Antonio. No especifíca;Fil: Campderrós, Mercedes Edith. No especifíca;Fil: Zaritzky, Noemi Elisabet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaIntechOpenElkordy, Amal Ali2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookParthttp://purl.org/coar/resource_type/c_3248info:ar-repo/semantics/parteDeLibroapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/148456Rodriguez Furlán, Laura Teresa; Lecot, Carlos Javier; Pérez Padilla, Antonio; Campderrós, Mercedes Edith; Zaritzky, Noemi Elisabet; Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins; IntechOpen; 2013; 197-218978-953-51-0947-1CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.intechopen.com/chapters/42266info:eu-repo/semantics/altIdentifier/doi/10.5772/54462info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:33Zoai:ri.conicet.gov.ar:11336/148456instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:33.942CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins
title Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins
spellingShingle Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins
Rodriguez Furlán, Laura Teresa
Inulin
Cryoprotector
Lyoprotector
Bovine plasma proteins
title_short Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins
title_full Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins
title_fullStr Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins
title_full_unstemmed Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins
title_sort Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins
dc.creator.none.fl_str_mv Rodriguez Furlán, Laura Teresa
Lecot, Carlos Javier
Pérez Padilla, Antonio
Campderrós, Mercedes Edith
Zaritzky, Noemi Elisabet
author Rodriguez Furlán, Laura Teresa
author_facet Rodriguez Furlán, Laura Teresa
Lecot, Carlos Javier
Pérez Padilla, Antonio
Campderrós, Mercedes Edith
Zaritzky, Noemi Elisabet
author_role author
author2 Lecot, Carlos Javier
Pérez Padilla, Antonio
Campderrós, Mercedes Edith
Zaritzky, Noemi Elisabet
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Elkordy, Amal Ali
dc.subject.none.fl_str_mv Inulin
Cryoprotector
Lyoprotector
Bovine plasma proteins
topic Inulin
Cryoprotector
Lyoprotector
Bovine plasma proteins
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Inulin is a generic term applied to heterogeneous blends of fructo-oligosaccharides which are reserve carbohydrate sources present in many plant foods such as bananas,onions, garlic, leeks, artichokes and chicory, which represents the main commercial source.This polysaccharide has a wide range of both, nutritional and technological applications.Nutritionally, inulin is regarded as a soluble fiber which promotes the growth of intestinalbacteria, acting as a prebiotic. Also, is a non-digestible carbohydrate with minimal impact on blood sugar and unlike fructose, it is not insulemic and does not raise triglycerides being generally considered suitable for diabetics and potentially helpful in managing blood sugarrelated illnesses . Among the technological benefits, inulin is used as fat and sugar replacement, low caloric bulking agent, texturing and water-binding agent . One general property of the saccharides is the stabilization of proteins by their incorporation into carbohydrate solutions before freeze-drying being this a known preservation procedure. The previous incorporation of saccharide promotes the formation of amorphous, glassy systems, inhibits crystallization and influences the kinetics of deteriorative reactions upon storage by which its structured integrity is maintained . To act successfully as a protectant, the saccharides should have a high glass transition temperature (Tg), a poor hygroscopicity, a low crystallization rate, containing no reducing groups. When freezedrying is envisaged as a method of drying, a relatively high T´g of the freeze concentrated fraction is preferable. Previous studies demonstrated that inulin meets these requirements being excellent protector of therapeutical proteins and viruses over the drying and storage processes .The glass transition temperatures of the maximally concentrated frozen solutions (T´g) wereanalyzed and compared to the experimental results by applying the predictive equations of Miller/Fox and Gordon/Taylor extended for multi-component systems. The glass transition (Tg) of the freeze dried multi-component mixtures, the onset crystallization temperature (Tc) of the solute at temperatures above Tg, in the freeze dried samples were determined.Furthermore, the kinetics of the denaturation and the thermal denaturation (Td) of the freeze-dried samples, at different DSC scan rates, protein concentrations and pH, were analyzed and the thermodynamic compatibility of the different matrix components were determined. The enthalpy change involved in the denaturation reactions of proteins was also determined. A kinetic model that describes bovine plasma proteins denaturation was proposed.
Fil: Rodriguez Furlán, Laura Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Lecot, Carlos Javier. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Pérez Padilla, Antonio. No especifíca;
Fil: Campderrós, Mercedes Edith. No especifíca;
Fil: Zaritzky, Noemi Elisabet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
description Inulin is a generic term applied to heterogeneous blends of fructo-oligosaccharides which are reserve carbohydrate sources present in many plant foods such as bananas,onions, garlic, leeks, artichokes and chicory, which represents the main commercial source.This polysaccharide has a wide range of both, nutritional and technological applications.Nutritionally, inulin is regarded as a soluble fiber which promotes the growth of intestinalbacteria, acting as a prebiotic. Also, is a non-digestible carbohydrate with minimal impact on blood sugar and unlike fructose, it is not insulemic and does not raise triglycerides being generally considered suitable for diabetics and potentially helpful in managing blood sugarrelated illnesses . Among the technological benefits, inulin is used as fat and sugar replacement, low caloric bulking agent, texturing and water-binding agent . One general property of the saccharides is the stabilization of proteins by their incorporation into carbohydrate solutions before freeze-drying being this a known preservation procedure. The previous incorporation of saccharide promotes the formation of amorphous, glassy systems, inhibits crystallization and influences the kinetics of deteriorative reactions upon storage by which its structured integrity is maintained . To act successfully as a protectant, the saccharides should have a high glass transition temperature (Tg), a poor hygroscopicity, a low crystallization rate, containing no reducing groups. When freezedrying is envisaged as a method of drying, a relatively high T´g of the freeze concentrated fraction is preferable. Previous studies demonstrated that inulin meets these requirements being excellent protector of therapeutical proteins and viruses over the drying and storage processes .The glass transition temperatures of the maximally concentrated frozen solutions (T´g) wereanalyzed and compared to the experimental results by applying the predictive equations of Miller/Fox and Gordon/Taylor extended for multi-component systems. The glass transition (Tg) of the freeze dried multi-component mixtures, the onset crystallization temperature (Tc) of the solute at temperatures above Tg, in the freeze dried samples were determined.Furthermore, the kinetics of the denaturation and the thermal denaturation (Td) of the freeze-dried samples, at different DSC scan rates, protein concentrations and pH, were analyzed and the thermodynamic compatibility of the different matrix components were determined. The enthalpy change involved in the denaturation reactions of proteins was also determined. A kinetic model that describes bovine plasma proteins denaturation was proposed.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/bookPart
http://purl.org/coar/resource_type/c_3248
info:ar-repo/semantics/parteDeLibro
status_str publishedVersion
format bookPart
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/148456
Rodriguez Furlán, Laura Teresa; Lecot, Carlos Javier; Pérez Padilla, Antonio; Campderrós, Mercedes Edith; Zaritzky, Noemi Elisabet; Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins; IntechOpen; 2013; 197-218
978-953-51-0947-1
CONICET Digital
CONICET
url http://hdl.handle.net/11336/148456
identifier_str_mv Rodriguez Furlán, Laura Teresa; Lecot, Carlos Javier; Pérez Padilla, Antonio; Campderrós, Mercedes Edith; Zaritzky, Noemi Elisabet; Calorimetric Study of Inulin as Cryo- and Lyoprotector of Bovine Plasma Proteins; IntechOpen; 2013; 197-218
978-953-51-0947-1
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.intechopen.com/chapters/42266
info:eu-repo/semantics/altIdentifier/doi/10.5772/54462
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv IntechOpen
publisher.none.fl_str_mv IntechOpen
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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