High-throughput chemometrically assisted flow-injection method for the simultaneous determination of multi-antiretrovirals in water

Autores
Paradina Fernández, Lesly Anniled; Brasca, Romina; Alcaraz, Mirta Raquel; Culzoni, Maria Julia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work is focused on the simultaneous determination of different nucleosides (lamivudine, abacavir, zidovudine) and non-nucleoside (nevirapine, efavirenz) reverse transcriptase inhibitors (antiretrovirals) in river, tap and well water by pH-gradient flow-injection analysis with diode-array detection (FIA-DAD) and further multivariate curve resolution-alternating least square (MCR-ALS) processing. Previous to the development of the analytical method with quantitative purpose, the acid-base characterization was conducted for all the analytes by determination of the experimental pKa. The pKa values, obtained by UV spectrometric titration coupled to MCR-ALS data modeling, were in agreement with those found in the literature, and, interestingly, a non-reported pKa of 12.27 was identified for nevirapine. Eventually, the analytical method was developed and its performance on the quantitation of the antiretrovirals was evaluated through its application to validation and environmental samples. The recovery studies showed values between 85 and 115% with relative errors of prediction below 10%. The figures of merit were satisfactory for all the studied drugs, with limits of detection between 25 and 40 μg L−1. Therefore, the proposed method, which is based on the generation of high-throughput data, is a simple and suitable alternative to determine the concentration of antiretroviral drugs in complex matrices, without pre-concentration or extraction steps. Additionally, MCR-ALS demonstrated to be a useful tool for solving mixtures of analytes with high spectral overlapping, in the presence of unknown interferences.
Fil: Paradina Fernández, Lesly Anniled. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Departamento de Química. Área Química Analítica; Argentina
Fil: Brasca, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Departamento de Química. Área Química Analítica; Argentina
Fil: Alcaraz, Mirta Raquel. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Departamento de Química. Área Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Culzoni, Maria Julia. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Departamento de Química. Área Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Materia
ANTIRETROVIRAL
ENVIRONMENTAL SAMPLES
FIA-DAD
MCR-ALS
PKA
QUANTITATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/176269
Acceder
id CONICETDig_59f24aed85e86d8844d90d14f2e8822e
oai_identifier_str oai:ri.conicet.gov.ar:11336/176269
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling High-throughput chemometrically assisted flow-injection method for the simultaneous determination of multi-antiretrovirals in waterParadina Fernández, Lesly AnniledBrasca, RominaAlcaraz, Mirta RaquelCulzoni, Maria JuliaANTIRETROVIRALENVIRONMENTAL SAMPLESFIA-DADMCR-ALSPKAQUANTITATIONhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This work is focused on the simultaneous determination of different nucleosides (lamivudine, abacavir, zidovudine) and non-nucleoside (nevirapine, efavirenz) reverse transcriptase inhibitors (antiretrovirals) in river, tap and well water by pH-gradient flow-injection analysis with diode-array detection (FIA-DAD) and further multivariate curve resolution-alternating least square (MCR-ALS) processing. Previous to the development of the analytical method with quantitative purpose, the acid-base characterization was conducted for all the analytes by determination of the experimental pKa. The pKa values, obtained by UV spectrometric titration coupled to MCR-ALS data modeling, were in agreement with those found in the literature, and, interestingly, a non-reported pKa of 12.27 was identified for nevirapine. Eventually, the analytical method was developed and its performance on the quantitation of the antiretrovirals was evaluated through its application to validation and environmental samples. The recovery studies showed values between 85 and 115% with relative errors of prediction below 10%. The figures of merit were satisfactory for all the studied drugs, with limits of detection between 25 and 40 μg L−1. Therefore, the proposed method, which is based on the generation of high-throughput data, is a simple and suitable alternative to determine the concentration of antiretroviral drugs in complex matrices, without pre-concentration or extraction steps. Additionally, MCR-ALS demonstrated to be a useful tool for solving mixtures of analytes with high spectral overlapping, in the presence of unknown interferences.Fil: Paradina Fernández, Lesly Anniled. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Departamento de Química. Área Química Analítica; ArgentinaFil: Brasca, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Departamento de Química. Área Química Analítica; ArgentinaFil: Alcaraz, Mirta Raquel. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Departamento de Química. Área Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Culzoni, Maria Julia. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Departamento de Química. Área Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaElsevier Science2018-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/176269Paradina Fernández, Lesly Anniled; Brasca, Romina; Alcaraz, Mirta Raquel; Culzoni, Maria Julia; High-throughput chemometrically assisted flow-injection method for the simultaneous determination of multi-antiretrovirals in water; Elsevier Science; Microchemical Journal; 141; 9-2018; 80-860026-265XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X18303370info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2018.05.011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:14Zoai:ri.conicet.gov.ar:11336/176269instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:14.448CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv High-throughput chemometrically assisted flow-injection method for the simultaneous determination of multi-antiretrovirals in water
title High-throughput chemometrically assisted flow-injection method for the simultaneous determination of multi-antiretrovirals in water
spellingShingle High-throughput chemometrically assisted flow-injection method for the simultaneous determination of multi-antiretrovirals in water
Paradina Fernández, Lesly Anniled
ANTIRETROVIRAL
ENVIRONMENTAL SAMPLES
FIA-DAD
MCR-ALS
PKA
QUANTITATION
title_short High-throughput chemometrically assisted flow-injection method for the simultaneous determination of multi-antiretrovirals in water
title_full High-throughput chemometrically assisted flow-injection method for the simultaneous determination of multi-antiretrovirals in water
title_fullStr High-throughput chemometrically assisted flow-injection method for the simultaneous determination of multi-antiretrovirals in water
title_full_unstemmed High-throughput chemometrically assisted flow-injection method for the simultaneous determination of multi-antiretrovirals in water
title_sort High-throughput chemometrically assisted flow-injection method for the simultaneous determination of multi-antiretrovirals in water
dc.creator.none.fl_str_mv Paradina Fernández, Lesly Anniled
Brasca, Romina
Alcaraz, Mirta Raquel
Culzoni, Maria Julia
author Paradina Fernández, Lesly Anniled
author_facet Paradina Fernández, Lesly Anniled
Brasca, Romina
Alcaraz, Mirta Raquel
Culzoni, Maria Julia
author_role author
author2 Brasca, Romina
Alcaraz, Mirta Raquel
Culzoni, Maria Julia
author2_role author
author
author
dc.subject.none.fl_str_mv ANTIRETROVIRAL
ENVIRONMENTAL SAMPLES
FIA-DAD
MCR-ALS
PKA
QUANTITATION
topic ANTIRETROVIRAL
ENVIRONMENTAL SAMPLES
FIA-DAD
MCR-ALS
PKA
QUANTITATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This work is focused on the simultaneous determination of different nucleosides (lamivudine, abacavir, zidovudine) and non-nucleoside (nevirapine, efavirenz) reverse transcriptase inhibitors (antiretrovirals) in river, tap and well water by pH-gradient flow-injection analysis with diode-array detection (FIA-DAD) and further multivariate curve resolution-alternating least square (MCR-ALS) processing. Previous to the development of the analytical method with quantitative purpose, the acid-base characterization was conducted for all the analytes by determination of the experimental pKa. The pKa values, obtained by UV spectrometric titration coupled to MCR-ALS data modeling, were in agreement with those found in the literature, and, interestingly, a non-reported pKa of 12.27 was identified for nevirapine. Eventually, the analytical method was developed and its performance on the quantitation of the antiretrovirals was evaluated through its application to validation and environmental samples. The recovery studies showed values between 85 and 115% with relative errors of prediction below 10%. The figures of merit were satisfactory for all the studied drugs, with limits of detection between 25 and 40 μg L−1. Therefore, the proposed method, which is based on the generation of high-throughput data, is a simple and suitable alternative to determine the concentration of antiretroviral drugs in complex matrices, without pre-concentration or extraction steps. Additionally, MCR-ALS demonstrated to be a useful tool for solving mixtures of analytes with high spectral overlapping, in the presence of unknown interferences.
Fil: Paradina Fernández, Lesly Anniled. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Departamento de Química. Área Química Analítica; Argentina
Fil: Brasca, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Departamento de Química. Área Química Analítica; Argentina
Fil: Alcaraz, Mirta Raquel. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Departamento de Química. Área Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Culzoni, Maria Julia. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Departamento de Química. Área Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
description This work is focused on the simultaneous determination of different nucleosides (lamivudine, abacavir, zidovudine) and non-nucleoside (nevirapine, efavirenz) reverse transcriptase inhibitors (antiretrovirals) in river, tap and well water by pH-gradient flow-injection analysis with diode-array detection (FIA-DAD) and further multivariate curve resolution-alternating least square (MCR-ALS) processing. Previous to the development of the analytical method with quantitative purpose, the acid-base characterization was conducted for all the analytes by determination of the experimental pKa. The pKa values, obtained by UV spectrometric titration coupled to MCR-ALS data modeling, were in agreement with those found in the literature, and, interestingly, a non-reported pKa of 12.27 was identified for nevirapine. Eventually, the analytical method was developed and its performance on the quantitation of the antiretrovirals was evaluated through its application to validation and environmental samples. The recovery studies showed values between 85 and 115% with relative errors of prediction below 10%. The figures of merit were satisfactory for all the studied drugs, with limits of detection between 25 and 40 μg L−1. Therefore, the proposed method, which is based on the generation of high-throughput data, is a simple and suitable alternative to determine the concentration of antiretroviral drugs in complex matrices, without pre-concentration or extraction steps. Additionally, MCR-ALS demonstrated to be a useful tool for solving mixtures of analytes with high spectral overlapping, in the presence of unknown interferences.
publishDate 2018
dc.date.none.fl_str_mv 2018-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/176269
Paradina Fernández, Lesly Anniled; Brasca, Romina; Alcaraz, Mirta Raquel; Culzoni, Maria Julia; High-throughput chemometrically assisted flow-injection method for the simultaneous determination of multi-antiretrovirals in water; Elsevier Science; Microchemical Journal; 141; 9-2018; 80-86
0026-265X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/176269
identifier_str_mv Paradina Fernández, Lesly Anniled; Brasca, Romina; Alcaraz, Mirta Raquel; Culzoni, Maria Julia; High-throughput chemometrically assisted flow-injection method for the simultaneous determination of multi-antiretrovirals in water; Elsevier Science; Microchemical Journal; 141; 9-2018; 80-86
0026-265X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X18303370
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2018.05.011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268651551457280
score 13.13397

Publicaciones similares