Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids
- Autores
- Toledano Tableros, José Eduardo; Gayosso Vázquez, Catalina; Jarillo Quijada, María Dolores; Fernández Vázquez, José Luis; Morfin Otero, Rayo; Rodríguez Noriega, Eduardo; Giono Cerezo, Silvia; Gutkind, Gabriel Osvaldo; Di Conza, José Alejandro; Santos Preciado, José Ignacio; Alcántar Curiel, María Dolores
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Nosocomial infections caused by multidrug-resistant (MDR) Klebsiella pneumoniae are a major health problem worldwide. The aim of this study was to describe NDM-1- producing K. pneumoniae strains causing bacteremia in a tertiary referral hospital in Mexico. MDR K. pneumoniae isolates were screened by polymerase chain reaction for the presence of resistance genes. In resistant isolates, plasmids were identified and conjugation assays were performed. Clonal diversity and the sequence types were determined by pulsed-field gel electrophoresis and multilocus sequence typing. A total of 80 K. pneumoniae isolates were collected from patients with bacteremia over a 1-year period. These isolates showed a level of resistance of 59% (47/80) to aztreonam, 56–60% (45–48/80) to cephalosporins, 54% (43/80) to colistin and 12.5% (10/80) to carbapenems. The carbapenem resistant isolates were blaNDM−1 carriers and negative for blaKPC, blaNDM, blaIMP, blaVIM and blaOXA−48−like carbapenemases genes. Conjugative plasmids IncFIIA and IncF group with sizes of 82–195 kbp were carriers of blaNDM−1, blaCTX−M−15, blaTEM−1, aac(60 )-Ib and/or aac(30 )-IIa. Clonal variability and nine different multilocus sequence types were detected (ST661, ST683, ST1395, ST2706, ST252, ST1198, ST690, ST1535, and ST3368) for the first time in the isolates carrying blaNDM−1 in Mexico. This study demonstrates that blaNDM−1 has remained within this hospital in recent years and suggests that it is currently the most prevalent carbapenemase among K. pneumoniae MDR strains causing bacteremia in Mexico. The horizontal transfer of blaNDM−1 gene through IncF-like plasmids among different clones demonstrates the dissemination pathway of antimicrobial resistance and underscore the need for strong and urgent joint measures to control the spread of NDM-1 carbapenemase in the hospital.
Fil: Toledano Tableros, José Eduardo. Universidad Nacional Autónoma de México; México. Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados. Departamento de Física; México
Fil: Gayosso Vázquez, Catalina. Universidad Nacional Autónoma de México; México
Fil: Jarillo Quijada, María Dolores. Universidad Nacional Autónoma de México; México
Fil: Fernández Vázquez, José Luis. Universidad Nacional Autónoma de México; México
Fil: Morfin Otero, Rayo. Universidad de Guadalajara; México
Fil: Rodríguez Noriega, Eduardo. Universidad de Guadalajara; México
Fil: Giono Cerezo, Silvia. Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados. Departamento de Física; México
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Di Conza, José Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Santos Preciado, José Ignacio. Universidad Nacional Autónoma de México; México
Fil: Alcántar Curiel, María Dolores. Universidad Nacional Autónoma de México; México - Materia
-
NDM-1
INCF-LIKE PLASMIDS
MEXICO
KLEBSIELLA PNEUMONIAE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/156890
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Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like PlasmidsToledano Tableros, José EduardoGayosso Vázquez, CatalinaJarillo Quijada, María DoloresFernández Vázquez, José LuisMorfin Otero, RayoRodríguez Noriega, EduardoGiono Cerezo, SilviaGutkind, Gabriel OsvaldoDi Conza, José AlejandroSantos Preciado, José IgnacioAlcántar Curiel, María DoloresNDM-1INCF-LIKE PLASMIDSMEXICOKLEBSIELLA PNEUMONIAEhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Nosocomial infections caused by multidrug-resistant (MDR) Klebsiella pneumoniae are a major health problem worldwide. The aim of this study was to describe NDM-1- producing K. pneumoniae strains causing bacteremia in a tertiary referral hospital in Mexico. MDR K. pneumoniae isolates were screened by polymerase chain reaction for the presence of resistance genes. In resistant isolates, plasmids were identified and conjugation assays were performed. Clonal diversity and the sequence types were determined by pulsed-field gel electrophoresis and multilocus sequence typing. A total of 80 K. pneumoniae isolates were collected from patients with bacteremia over a 1-year period. These isolates showed a level of resistance of 59% (47/80) to aztreonam, 56–60% (45–48/80) to cephalosporins, 54% (43/80) to colistin and 12.5% (10/80) to carbapenems. The carbapenem resistant isolates were blaNDM−1 carriers and negative for blaKPC, blaNDM, blaIMP, blaVIM and blaOXA−48−like carbapenemases genes. Conjugative plasmids IncFIIA and IncF group with sizes of 82–195 kbp were carriers of blaNDM−1, blaCTX−M−15, blaTEM−1, aac(60 )-Ib and/or aac(30 )-IIa. Clonal variability and nine different multilocus sequence types were detected (ST661, ST683, ST1395, ST2706, ST252, ST1198, ST690, ST1535, and ST3368) for the first time in the isolates carrying blaNDM−1 in Mexico. This study demonstrates that blaNDM−1 has remained within this hospital in recent years and suggests that it is currently the most prevalent carbapenemase among K. pneumoniae MDR strains causing bacteremia in Mexico. The horizontal transfer of blaNDM−1 gene through IncF-like plasmids among different clones demonstrates the dissemination pathway of antimicrobial resistance and underscore the need for strong and urgent joint measures to control the spread of NDM-1 carbapenemase in the hospital.Fil: Toledano Tableros, José Eduardo. Universidad Nacional Autónoma de México; México. Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados. Departamento de Física; MéxicoFil: Gayosso Vázquez, Catalina. Universidad Nacional Autónoma de México; MéxicoFil: Jarillo Quijada, María Dolores. Universidad Nacional Autónoma de México; MéxicoFil: Fernández Vázquez, José Luis. Universidad Nacional Autónoma de México; MéxicoFil: Morfin Otero, Rayo. Universidad de Guadalajara; MéxicoFil: Rodríguez Noriega, Eduardo. Universidad de Guadalajara; MéxicoFil: Giono Cerezo, Silvia. Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados. Departamento de Física; MéxicoFil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Di Conza, José Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Santos Preciado, José Ignacio. Universidad Nacional Autónoma de México; MéxicoFil: Alcántar Curiel, María Dolores. Universidad Nacional Autónoma de México; MéxicoFrontiers Media2021-03-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/156890Toledano Tableros, José Eduardo; Gayosso Vázquez, Catalina; Jarillo Quijada, María Dolores; Fernández Vázquez, José Luis; Morfin Otero, Rayo; et al.; Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids; Frontiers Media; Frontiers in Microbiology; 12; 25-3-2021; 1-91664-302X1664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2021.611274/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2021.611274info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:27Zoai:ri.conicet.gov.ar:11336/156890instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:27.384CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids |
title |
Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids |
spellingShingle |
Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids Toledano Tableros, José Eduardo NDM-1 INCF-LIKE PLASMIDS MEXICO KLEBSIELLA PNEUMONIAE |
title_short |
Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids |
title_full |
Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids |
title_fullStr |
Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids |
title_full_unstemmed |
Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids |
title_sort |
Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids |
dc.creator.none.fl_str_mv |
Toledano Tableros, José Eduardo Gayosso Vázquez, Catalina Jarillo Quijada, María Dolores Fernández Vázquez, José Luis Morfin Otero, Rayo Rodríguez Noriega, Eduardo Giono Cerezo, Silvia Gutkind, Gabriel Osvaldo Di Conza, José Alejandro Santos Preciado, José Ignacio Alcántar Curiel, María Dolores |
author |
Toledano Tableros, José Eduardo |
author_facet |
Toledano Tableros, José Eduardo Gayosso Vázquez, Catalina Jarillo Quijada, María Dolores Fernández Vázquez, José Luis Morfin Otero, Rayo Rodríguez Noriega, Eduardo Giono Cerezo, Silvia Gutkind, Gabriel Osvaldo Di Conza, José Alejandro Santos Preciado, José Ignacio Alcántar Curiel, María Dolores |
author_role |
author |
author2 |
Gayosso Vázquez, Catalina Jarillo Quijada, María Dolores Fernández Vázquez, José Luis Morfin Otero, Rayo Rodríguez Noriega, Eduardo Giono Cerezo, Silvia Gutkind, Gabriel Osvaldo Di Conza, José Alejandro Santos Preciado, José Ignacio Alcántar Curiel, María Dolores |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
NDM-1 INCF-LIKE PLASMIDS MEXICO KLEBSIELLA PNEUMONIAE |
topic |
NDM-1 INCF-LIKE PLASMIDS MEXICO KLEBSIELLA PNEUMONIAE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Nosocomial infections caused by multidrug-resistant (MDR) Klebsiella pneumoniae are a major health problem worldwide. The aim of this study was to describe NDM-1- producing K. pneumoniae strains causing bacteremia in a tertiary referral hospital in Mexico. MDR K. pneumoniae isolates were screened by polymerase chain reaction for the presence of resistance genes. In resistant isolates, plasmids were identified and conjugation assays were performed. Clonal diversity and the sequence types were determined by pulsed-field gel electrophoresis and multilocus sequence typing. A total of 80 K. pneumoniae isolates were collected from patients with bacteremia over a 1-year period. These isolates showed a level of resistance of 59% (47/80) to aztreonam, 56–60% (45–48/80) to cephalosporins, 54% (43/80) to colistin and 12.5% (10/80) to carbapenems. The carbapenem resistant isolates were blaNDM−1 carriers and negative for blaKPC, blaNDM, blaIMP, blaVIM and blaOXA−48−like carbapenemases genes. Conjugative plasmids IncFIIA and IncF group with sizes of 82–195 kbp were carriers of blaNDM−1, blaCTX−M−15, blaTEM−1, aac(60 )-Ib and/or aac(30 )-IIa. Clonal variability and nine different multilocus sequence types were detected (ST661, ST683, ST1395, ST2706, ST252, ST1198, ST690, ST1535, and ST3368) for the first time in the isolates carrying blaNDM−1 in Mexico. This study demonstrates that blaNDM−1 has remained within this hospital in recent years and suggests that it is currently the most prevalent carbapenemase among K. pneumoniae MDR strains causing bacteremia in Mexico. The horizontal transfer of blaNDM−1 gene through IncF-like plasmids among different clones demonstrates the dissemination pathway of antimicrobial resistance and underscore the need for strong and urgent joint measures to control the spread of NDM-1 carbapenemase in the hospital. Fil: Toledano Tableros, José Eduardo. Universidad Nacional Autónoma de México; México. Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados. Departamento de Física; México Fil: Gayosso Vázquez, Catalina. Universidad Nacional Autónoma de México; México Fil: Jarillo Quijada, María Dolores. Universidad Nacional Autónoma de México; México Fil: Fernández Vázquez, José Luis. Universidad Nacional Autónoma de México; México Fil: Morfin Otero, Rayo. Universidad de Guadalajara; México Fil: Rodríguez Noriega, Eduardo. Universidad de Guadalajara; México Fil: Giono Cerezo, Silvia. Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados. Departamento de Física; México Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Di Conza, José Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Santos Preciado, José Ignacio. Universidad Nacional Autónoma de México; México Fil: Alcántar Curiel, María Dolores. Universidad Nacional Autónoma de México; México |
description |
Nosocomial infections caused by multidrug-resistant (MDR) Klebsiella pneumoniae are a major health problem worldwide. The aim of this study was to describe NDM-1- producing K. pneumoniae strains causing bacteremia in a tertiary referral hospital in Mexico. MDR K. pneumoniae isolates were screened by polymerase chain reaction for the presence of resistance genes. In resistant isolates, plasmids were identified and conjugation assays were performed. Clonal diversity and the sequence types were determined by pulsed-field gel electrophoresis and multilocus sequence typing. A total of 80 K. pneumoniae isolates were collected from patients with bacteremia over a 1-year period. These isolates showed a level of resistance of 59% (47/80) to aztreonam, 56–60% (45–48/80) to cephalosporins, 54% (43/80) to colistin and 12.5% (10/80) to carbapenems. The carbapenem resistant isolates were blaNDM−1 carriers and negative for blaKPC, blaNDM, blaIMP, blaVIM and blaOXA−48−like carbapenemases genes. Conjugative plasmids IncFIIA and IncF group with sizes of 82–195 kbp were carriers of blaNDM−1, blaCTX−M−15, blaTEM−1, aac(60 )-Ib and/or aac(30 )-IIa. Clonal variability and nine different multilocus sequence types were detected (ST661, ST683, ST1395, ST2706, ST252, ST1198, ST690, ST1535, and ST3368) for the first time in the isolates carrying blaNDM−1 in Mexico. This study demonstrates that blaNDM−1 has remained within this hospital in recent years and suggests that it is currently the most prevalent carbapenemase among K. pneumoniae MDR strains causing bacteremia in Mexico. The horizontal transfer of blaNDM−1 gene through IncF-like plasmids among different clones demonstrates the dissemination pathway of antimicrobial resistance and underscore the need for strong and urgent joint measures to control the spread of NDM-1 carbapenemase in the hospital. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-03-25 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/156890 Toledano Tableros, José Eduardo; Gayosso Vázquez, Catalina; Jarillo Quijada, María Dolores; Fernández Vázquez, José Luis; Morfin Otero, Rayo; et al.; Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids; Frontiers Media; Frontiers in Microbiology; 12; 25-3-2021; 1-9 1664-302X 1664-302X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/156890 |
identifier_str_mv |
Toledano Tableros, José Eduardo; Gayosso Vázquez, Catalina; Jarillo Quijada, María Dolores; Fernández Vázquez, José Luis; Morfin Otero, Rayo; et al.; Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids; Frontiers Media; Frontiers in Microbiology; 12; 25-3-2021; 1-9 1664-302X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2021.611274/full info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2021.611274 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |