Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids

Autores
Toledano Tableros, José Eduardo; Gayosso Vázquez, Catalina; Jarillo Quijada, María Dolores; Fernández Vázquez, José Luis; Morfin Otero, Rayo; Rodríguez Noriega, Eduardo; Giono Cerezo, Silvia; Gutkind, Gabriel Osvaldo; Di Conza, José Alejandro; Santos Preciado, José Ignacio; Alcántar Curiel, María Dolores
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nosocomial infections caused by multidrug-resistant (MDR) Klebsiella pneumoniae are a major health problem worldwide. The aim of this study was to describe NDM-1- producing K. pneumoniae strains causing bacteremia in a tertiary referral hospital in Mexico. MDR K. pneumoniae isolates were screened by polymerase chain reaction for the presence of resistance genes. In resistant isolates, plasmids were identified and conjugation assays were performed. Clonal diversity and the sequence types were determined by pulsed-field gel electrophoresis and multilocus sequence typing. A total of 80 K. pneumoniae isolates were collected from patients with bacteremia over a 1-year period. These isolates showed a level of resistance of 59% (47/80) to aztreonam, 56–60% (45–48/80) to cephalosporins, 54% (43/80) to colistin and 12.5% (10/80) to carbapenems. The carbapenem resistant isolates were blaNDM−1 carriers and negative for blaKPC, blaNDM, blaIMP, blaVIM and blaOXA−48−like carbapenemases genes. Conjugative plasmids IncFIIA and IncF group with sizes of 82–195 kbp were carriers of blaNDM−1, blaCTX−M−15, blaTEM−1, aac(60 )-Ib and/or aac(30 )-IIa. Clonal variability and nine different multilocus sequence types were detected (ST661, ST683, ST1395, ST2706, ST252, ST1198, ST690, ST1535, and ST3368) for the first time in the isolates carrying blaNDM−1 in Mexico. This study demonstrates that blaNDM−1 has remained within this hospital in recent years and suggests that it is currently the most prevalent carbapenemase among K. pneumoniae MDR strains causing bacteremia in Mexico. The horizontal transfer of blaNDM−1 gene through IncF-like plasmids among different clones demonstrates the dissemination pathway of antimicrobial resistance and underscore the need for strong and urgent joint measures to control the spread of NDM-1 carbapenemase in the hospital.
Fil: Toledano Tableros, José Eduardo. Universidad Nacional Autónoma de México; México. Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados. Departamento de Física; México
Fil: Gayosso Vázquez, Catalina. Universidad Nacional Autónoma de México; México
Fil: Jarillo Quijada, María Dolores. Universidad Nacional Autónoma de México; México
Fil: Fernández Vázquez, José Luis. Universidad Nacional Autónoma de México; México
Fil: Morfin Otero, Rayo. Universidad de Guadalajara; México
Fil: Rodríguez Noriega, Eduardo. Universidad de Guadalajara; México
Fil: Giono Cerezo, Silvia. Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados. Departamento de Física; México
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Di Conza, José Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Santos Preciado, José Ignacio. Universidad Nacional Autónoma de México; México
Fil: Alcántar Curiel, María Dolores. Universidad Nacional Autónoma de México; México
Materia
NDM-1
INCF-LIKE PLASMIDS
MEXICO
KLEBSIELLA PNEUMONIAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/156890

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spelling Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like PlasmidsToledano Tableros, José EduardoGayosso Vázquez, CatalinaJarillo Quijada, María DoloresFernández Vázquez, José LuisMorfin Otero, RayoRodríguez Noriega, EduardoGiono Cerezo, SilviaGutkind, Gabriel OsvaldoDi Conza, José AlejandroSantos Preciado, José IgnacioAlcántar Curiel, María DoloresNDM-1INCF-LIKE PLASMIDSMEXICOKLEBSIELLA PNEUMONIAEhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Nosocomial infections caused by multidrug-resistant (MDR) Klebsiella pneumoniae are a major health problem worldwide. The aim of this study was to describe NDM-1- producing K. pneumoniae strains causing bacteremia in a tertiary referral hospital in Mexico. MDR K. pneumoniae isolates were screened by polymerase chain reaction for the presence of resistance genes. In resistant isolates, plasmids were identified and conjugation assays were performed. Clonal diversity and the sequence types were determined by pulsed-field gel electrophoresis and multilocus sequence typing. A total of 80 K. pneumoniae isolates were collected from patients with bacteremia over a 1-year period. These isolates showed a level of resistance of 59% (47/80) to aztreonam, 56–60% (45–48/80) to cephalosporins, 54% (43/80) to colistin and 12.5% (10/80) to carbapenems. The carbapenem resistant isolates were blaNDM−1 carriers and negative for blaKPC, blaNDM, blaIMP, blaVIM and blaOXA−48−like carbapenemases genes. Conjugative plasmids IncFIIA and IncF group with sizes of 82–195 kbp were carriers of blaNDM−1, blaCTX−M−15, blaTEM−1, aac(60 )-Ib and/or aac(30 )-IIa. Clonal variability and nine different multilocus sequence types were detected (ST661, ST683, ST1395, ST2706, ST252, ST1198, ST690, ST1535, and ST3368) for the first time in the isolates carrying blaNDM−1 in Mexico. This study demonstrates that blaNDM−1 has remained within this hospital in recent years and suggests that it is currently the most prevalent carbapenemase among K. pneumoniae MDR strains causing bacteremia in Mexico. The horizontal transfer of blaNDM−1 gene through IncF-like plasmids among different clones demonstrates the dissemination pathway of antimicrobial resistance and underscore the need for strong and urgent joint measures to control the spread of NDM-1 carbapenemase in the hospital.Fil: Toledano Tableros, José Eduardo. Universidad Nacional Autónoma de México; México. Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados. Departamento de Física; MéxicoFil: Gayosso Vázquez, Catalina. Universidad Nacional Autónoma de México; MéxicoFil: Jarillo Quijada, María Dolores. Universidad Nacional Autónoma de México; MéxicoFil: Fernández Vázquez, José Luis. Universidad Nacional Autónoma de México; MéxicoFil: Morfin Otero, Rayo. Universidad de Guadalajara; MéxicoFil: Rodríguez Noriega, Eduardo. Universidad de Guadalajara; MéxicoFil: Giono Cerezo, Silvia. Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados. Departamento de Física; MéxicoFil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Di Conza, José Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Santos Preciado, José Ignacio. Universidad Nacional Autónoma de México; MéxicoFil: Alcántar Curiel, María Dolores. Universidad Nacional Autónoma de México; MéxicoFrontiers Media2021-03-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/156890Toledano Tableros, José Eduardo; Gayosso Vázquez, Catalina; Jarillo Quijada, María Dolores; Fernández Vázquez, José Luis; Morfin Otero, Rayo; et al.; Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids; Frontiers Media; Frontiers in Microbiology; 12; 25-3-2021; 1-91664-302X1664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2021.611274/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2021.611274info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:27Zoai:ri.conicet.gov.ar:11336/156890instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:27.384CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids
title Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids
spellingShingle Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids
Toledano Tableros, José Eduardo
NDM-1
INCF-LIKE PLASMIDS
MEXICO
KLEBSIELLA PNEUMONIAE
title_short Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids
title_full Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids
title_fullStr Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids
title_full_unstemmed Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids
title_sort Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids
dc.creator.none.fl_str_mv Toledano Tableros, José Eduardo
Gayosso Vázquez, Catalina
Jarillo Quijada, María Dolores
Fernández Vázquez, José Luis
Morfin Otero, Rayo
Rodríguez Noriega, Eduardo
Giono Cerezo, Silvia
Gutkind, Gabriel Osvaldo
Di Conza, José Alejandro
Santos Preciado, José Ignacio
Alcántar Curiel, María Dolores
author Toledano Tableros, José Eduardo
author_facet Toledano Tableros, José Eduardo
Gayosso Vázquez, Catalina
Jarillo Quijada, María Dolores
Fernández Vázquez, José Luis
Morfin Otero, Rayo
Rodríguez Noriega, Eduardo
Giono Cerezo, Silvia
Gutkind, Gabriel Osvaldo
Di Conza, José Alejandro
Santos Preciado, José Ignacio
Alcántar Curiel, María Dolores
author_role author
author2 Gayosso Vázquez, Catalina
Jarillo Quijada, María Dolores
Fernández Vázquez, José Luis
Morfin Otero, Rayo
Rodríguez Noriega, Eduardo
Giono Cerezo, Silvia
Gutkind, Gabriel Osvaldo
Di Conza, José Alejandro
Santos Preciado, José Ignacio
Alcántar Curiel, María Dolores
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv NDM-1
INCF-LIKE PLASMIDS
MEXICO
KLEBSIELLA PNEUMONIAE
topic NDM-1
INCF-LIKE PLASMIDS
MEXICO
KLEBSIELLA PNEUMONIAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Nosocomial infections caused by multidrug-resistant (MDR) Klebsiella pneumoniae are a major health problem worldwide. The aim of this study was to describe NDM-1- producing K. pneumoniae strains causing bacteremia in a tertiary referral hospital in Mexico. MDR K. pneumoniae isolates were screened by polymerase chain reaction for the presence of resistance genes. In resistant isolates, plasmids were identified and conjugation assays were performed. Clonal diversity and the sequence types were determined by pulsed-field gel electrophoresis and multilocus sequence typing. A total of 80 K. pneumoniae isolates were collected from patients with bacteremia over a 1-year period. These isolates showed a level of resistance of 59% (47/80) to aztreonam, 56–60% (45–48/80) to cephalosporins, 54% (43/80) to colistin and 12.5% (10/80) to carbapenems. The carbapenem resistant isolates were blaNDM−1 carriers and negative for blaKPC, blaNDM, blaIMP, blaVIM and blaOXA−48−like carbapenemases genes. Conjugative plasmids IncFIIA and IncF group with sizes of 82–195 kbp were carriers of blaNDM−1, blaCTX−M−15, blaTEM−1, aac(60 )-Ib and/or aac(30 )-IIa. Clonal variability and nine different multilocus sequence types were detected (ST661, ST683, ST1395, ST2706, ST252, ST1198, ST690, ST1535, and ST3368) for the first time in the isolates carrying blaNDM−1 in Mexico. This study demonstrates that blaNDM−1 has remained within this hospital in recent years and suggests that it is currently the most prevalent carbapenemase among K. pneumoniae MDR strains causing bacteremia in Mexico. The horizontal transfer of blaNDM−1 gene through IncF-like plasmids among different clones demonstrates the dissemination pathway of antimicrobial resistance and underscore the need for strong and urgent joint measures to control the spread of NDM-1 carbapenemase in the hospital.
Fil: Toledano Tableros, José Eduardo. Universidad Nacional Autónoma de México; México. Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados. Departamento de Física; México
Fil: Gayosso Vázquez, Catalina. Universidad Nacional Autónoma de México; México
Fil: Jarillo Quijada, María Dolores. Universidad Nacional Autónoma de México; México
Fil: Fernández Vázquez, José Luis. Universidad Nacional Autónoma de México; México
Fil: Morfin Otero, Rayo. Universidad de Guadalajara; México
Fil: Rodríguez Noriega, Eduardo. Universidad de Guadalajara; México
Fil: Giono Cerezo, Silvia. Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados. Departamento de Física; México
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Di Conza, José Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Santos Preciado, José Ignacio. Universidad Nacional Autónoma de México; México
Fil: Alcántar Curiel, María Dolores. Universidad Nacional Autónoma de México; México
description Nosocomial infections caused by multidrug-resistant (MDR) Klebsiella pneumoniae are a major health problem worldwide. The aim of this study was to describe NDM-1- producing K. pneumoniae strains causing bacteremia in a tertiary referral hospital in Mexico. MDR K. pneumoniae isolates were screened by polymerase chain reaction for the presence of resistance genes. In resistant isolates, plasmids were identified and conjugation assays were performed. Clonal diversity and the sequence types were determined by pulsed-field gel electrophoresis and multilocus sequence typing. A total of 80 K. pneumoniae isolates were collected from patients with bacteremia over a 1-year period. These isolates showed a level of resistance of 59% (47/80) to aztreonam, 56–60% (45–48/80) to cephalosporins, 54% (43/80) to colistin and 12.5% (10/80) to carbapenems. The carbapenem resistant isolates were blaNDM−1 carriers and negative for blaKPC, blaNDM, blaIMP, blaVIM and blaOXA−48−like carbapenemases genes. Conjugative plasmids IncFIIA and IncF group with sizes of 82–195 kbp were carriers of blaNDM−1, blaCTX−M−15, blaTEM−1, aac(60 )-Ib and/or aac(30 )-IIa. Clonal variability and nine different multilocus sequence types were detected (ST661, ST683, ST1395, ST2706, ST252, ST1198, ST690, ST1535, and ST3368) for the first time in the isolates carrying blaNDM−1 in Mexico. This study demonstrates that blaNDM−1 has remained within this hospital in recent years and suggests that it is currently the most prevalent carbapenemase among K. pneumoniae MDR strains causing bacteremia in Mexico. The horizontal transfer of blaNDM−1 gene through IncF-like plasmids among different clones demonstrates the dissemination pathway of antimicrobial resistance and underscore the need for strong and urgent joint measures to control the spread of NDM-1 carbapenemase in the hospital.
publishDate 2021
dc.date.none.fl_str_mv 2021-03-25
dc.type.none.fl_str_mv info:eu-repo/semantics/article
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/156890
Toledano Tableros, José Eduardo; Gayosso Vázquez, Catalina; Jarillo Quijada, María Dolores; Fernández Vázquez, José Luis; Morfin Otero, Rayo; et al.; Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids; Frontiers Media; Frontiers in Microbiology; 12; 25-3-2021; 1-9
1664-302X
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/156890
identifier_str_mv Toledano Tableros, José Eduardo; Gayosso Vázquez, Catalina; Jarillo Quijada, María Dolores; Fernández Vázquez, José Luis; Morfin Otero, Rayo; et al.; Dissemination of blaNDM–1 Gene Among Several Klebsiella pneumoniae Sequence Types in Mexico Associated With Horizontal Transfer Mediated by IncF-Like Plasmids; Frontiers Media; Frontiers in Microbiology; 12; 25-3-2021; 1-9
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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