PPAR ligands improve impaired metabolic pathways in fetal hearts of diabetic rats

Autores
Kurtz, Melisa Lidia Amelia; Martínez, Nora Alicia; Roberti, Sabrina Lorena; Arany, Edith; Jawerbaum, Alicia Sandra
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In maternal diabetes, the fetal heart can be structurally and functionally affected. Maternal diets enriched in certain unsaturated fatty acids can activate the nuclear receptors peroxisome proliferator-activated receptors (PPARs) and regulate metabolic and anti-inflammatory pathways during development. Our aim was to investigate whether PPARa expression, lipid metabolism, lipoperoxidation, andnitricoxide(NO) productionare alteredinthe fetal hearts of diabetic rats, and to analyze the putative effects of in vivo PPARactivation on these parameters. We found decreased PPARa expression in the hearts ofmale but not female fetuses of diabetic rats when compared with controls. Fetal treatments with the PPARa ligand leukotriene B4upregulated the expression of PPARα and target genes involved in fatty acid oxidation in the fetal hearts. Increased concentrations of triglycerides, cholesterol, and phospholipids were found in the hearts of fetuses of diabetic rats. Maternal treatments with diets supplemented with6%oliveoil or6%safflower oil,enrichedinunsaturatedfatty acids that canactivate PPARs, led to few changes in lipid concentrations, but up-regulated PPARa expression in fetal hearts. NO production, which was increased in the hearts of male and female fetuses in the diabetic group, and lipoperoxidation, which was increased in the hearts ofmale fetuses in the diabetic group, was reduced by thematernal treatments supplementedwithsaffloweroil. In conclusion, impaired PPARa expression, altered lipid metabolism, and increased oxidative and nitridergic pathways were evidenced in hearts of fetuses of diabetic rats and were regulated in a genderdependent manner by treatments enriched with PPAR ligands.
Fil: Kurtz, Melisa Lidia Amelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Martínez, Nora Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Roberti, Sabrina Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Arany, Edith. Western University; Canadá
Fil: Jawerbaum, Alicia Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Materia
DIABETES IN PREGNANCY
FETUS
HEART
NITRIC OXIDE
PPAR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/182669
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/182669
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling PPAR ligands improve impaired metabolic pathways in fetal hearts of diabetic ratsKurtz, Melisa Lidia AmeliaMartínez, Nora AliciaRoberti, Sabrina LorenaArany, EdithJawerbaum, Alicia SandraDIABETES IN PREGNANCYFETUSHEARTNITRIC OXIDEPPARhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In maternal diabetes, the fetal heart can be structurally and functionally affected. Maternal diets enriched in certain unsaturated fatty acids can activate the nuclear receptors peroxisome proliferator-activated receptors (PPARs) and regulate metabolic and anti-inflammatory pathways during development. Our aim was to investigate whether PPARa expression, lipid metabolism, lipoperoxidation, andnitricoxide(NO) productionare alteredinthe fetal hearts of diabetic rats, and to analyze the putative effects of in vivo PPARactivation on these parameters. We found decreased PPARa expression in the hearts ofmale but not female fetuses of diabetic rats when compared with controls. Fetal treatments with the PPARa ligand leukotriene B4upregulated the expression of PPARα and target genes involved in fatty acid oxidation in the fetal hearts. Increased concentrations of triglycerides, cholesterol, and phospholipids were found in the hearts of fetuses of diabetic rats. Maternal treatments with diets supplemented with6%oliveoil or6%safflower oil,enrichedinunsaturatedfatty acids that canactivate PPARs, led to few changes in lipid concentrations, but up-regulated PPARa expression in fetal hearts. NO production, which was increased in the hearts of male and female fetuses in the diabetic group, and lipoperoxidation, which was increased in the hearts ofmale fetuses in the diabetic group, was reduced by thematernal treatments supplementedwithsaffloweroil. In conclusion, impaired PPARa expression, altered lipid metabolism, and increased oxidative and nitridergic pathways were evidenced in hearts of fetuses of diabetic rats and were regulated in a genderdependent manner by treatments enriched with PPAR ligands.Fil: Kurtz, Melisa Lidia Amelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Martínez, Nora Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Roberti, Sabrina Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Arany, Edith. Western University; CanadáFil: Jawerbaum, Alicia Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaBioScientifica2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/182669Kurtz, Melisa Lidia Amelia; Martínez, Nora Alicia; Roberti, Sabrina Lorena; Arany, Edith; Jawerbaum, Alicia Sandra; PPAR ligands improve impaired metabolic pathways in fetal hearts of diabetic rats; BioScientifica; Journal of Molecular Endocrinology; 53; 2; 8-2014; 237-2460952-50411479-6813CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://jme.bioscientifica.com/view/journals/jme/53/2/237.xmlinfo:eu-repo/semantics/altIdentifier/doi/10.1530/JME-14-0063info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:37:25Zoai:ri.conicet.gov.ar:11336/182669instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:37:26.151CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv PPAR ligands improve impaired metabolic pathways in fetal hearts of diabetic rats
title PPAR ligands improve impaired metabolic pathways in fetal hearts of diabetic rats
spellingShingle PPAR ligands improve impaired metabolic pathways in fetal hearts of diabetic rats
Kurtz, Melisa Lidia Amelia
DIABETES IN PREGNANCY
FETUS
HEART
NITRIC OXIDE
PPAR
title_short PPAR ligands improve impaired metabolic pathways in fetal hearts of diabetic rats
title_full PPAR ligands improve impaired metabolic pathways in fetal hearts of diabetic rats
title_fullStr PPAR ligands improve impaired metabolic pathways in fetal hearts of diabetic rats
title_full_unstemmed PPAR ligands improve impaired metabolic pathways in fetal hearts of diabetic rats
title_sort PPAR ligands improve impaired metabolic pathways in fetal hearts of diabetic rats
dc.creator.none.fl_str_mv Kurtz, Melisa Lidia Amelia
Martínez, Nora Alicia
Roberti, Sabrina Lorena
Arany, Edith
Jawerbaum, Alicia Sandra
author Kurtz, Melisa Lidia Amelia
author_facet Kurtz, Melisa Lidia Amelia
Martínez, Nora Alicia
Roberti, Sabrina Lorena
Arany, Edith
Jawerbaum, Alicia Sandra
author_role author
author2 Martínez, Nora Alicia
Roberti, Sabrina Lorena
Arany, Edith
Jawerbaum, Alicia Sandra
author2_role author
author
author
author
dc.subject.none.fl_str_mv DIABETES IN PREGNANCY
FETUS
HEART
NITRIC OXIDE
PPAR
topic DIABETES IN PREGNANCY
FETUS
HEART
NITRIC OXIDE
PPAR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In maternal diabetes, the fetal heart can be structurally and functionally affected. Maternal diets enriched in certain unsaturated fatty acids can activate the nuclear receptors peroxisome proliferator-activated receptors (PPARs) and regulate metabolic and anti-inflammatory pathways during development. Our aim was to investigate whether PPARa expression, lipid metabolism, lipoperoxidation, andnitricoxide(NO) productionare alteredinthe fetal hearts of diabetic rats, and to analyze the putative effects of in vivo PPARactivation on these parameters. We found decreased PPARa expression in the hearts ofmale but not female fetuses of diabetic rats when compared with controls. Fetal treatments with the PPARa ligand leukotriene B4upregulated the expression of PPARα and target genes involved in fatty acid oxidation in the fetal hearts. Increased concentrations of triglycerides, cholesterol, and phospholipids were found in the hearts of fetuses of diabetic rats. Maternal treatments with diets supplemented with6%oliveoil or6%safflower oil,enrichedinunsaturatedfatty acids that canactivate PPARs, led to few changes in lipid concentrations, but up-regulated PPARa expression in fetal hearts. NO production, which was increased in the hearts of male and female fetuses in the diabetic group, and lipoperoxidation, which was increased in the hearts ofmale fetuses in the diabetic group, was reduced by thematernal treatments supplementedwithsaffloweroil. In conclusion, impaired PPARa expression, altered lipid metabolism, and increased oxidative and nitridergic pathways were evidenced in hearts of fetuses of diabetic rats and were regulated in a genderdependent manner by treatments enriched with PPAR ligands.
Fil: Kurtz, Melisa Lidia Amelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Martínez, Nora Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Roberti, Sabrina Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Arany, Edith. Western University; Canadá
Fil: Jawerbaum, Alicia Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
description In maternal diabetes, the fetal heart can be structurally and functionally affected. Maternal diets enriched in certain unsaturated fatty acids can activate the nuclear receptors peroxisome proliferator-activated receptors (PPARs) and regulate metabolic and anti-inflammatory pathways during development. Our aim was to investigate whether PPARa expression, lipid metabolism, lipoperoxidation, andnitricoxide(NO) productionare alteredinthe fetal hearts of diabetic rats, and to analyze the putative effects of in vivo PPARactivation on these parameters. We found decreased PPARa expression in the hearts ofmale but not female fetuses of diabetic rats when compared with controls. Fetal treatments with the PPARa ligand leukotriene B4upregulated the expression of PPARα and target genes involved in fatty acid oxidation in the fetal hearts. Increased concentrations of triglycerides, cholesterol, and phospholipids were found in the hearts of fetuses of diabetic rats. Maternal treatments with diets supplemented with6%oliveoil or6%safflower oil,enrichedinunsaturatedfatty acids that canactivate PPARs, led to few changes in lipid concentrations, but up-regulated PPARa expression in fetal hearts. NO production, which was increased in the hearts of male and female fetuses in the diabetic group, and lipoperoxidation, which was increased in the hearts ofmale fetuses in the diabetic group, was reduced by thematernal treatments supplementedwithsaffloweroil. In conclusion, impaired PPARa expression, altered lipid metabolism, and increased oxidative and nitridergic pathways were evidenced in hearts of fetuses of diabetic rats and were regulated in a genderdependent manner by treatments enriched with PPAR ligands.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/182669
Kurtz, Melisa Lidia Amelia; Martínez, Nora Alicia; Roberti, Sabrina Lorena; Arany, Edith; Jawerbaum, Alicia Sandra; PPAR ligands improve impaired metabolic pathways in fetal hearts of diabetic rats; BioScientifica; Journal of Molecular Endocrinology; 53; 2; 8-2014; 237-246
0952-5041
1479-6813
CONICET Digital
CONICET
url http://hdl.handle.net/11336/182669
identifier_str_mv Kurtz, Melisa Lidia Amelia; Martínez, Nora Alicia; Roberti, Sabrina Lorena; Arany, Edith; Jawerbaum, Alicia Sandra; PPAR ligands improve impaired metabolic pathways in fetal hearts of diabetic rats; BioScientifica; Journal of Molecular Endocrinology; 53; 2; 8-2014; 237-246
0952-5041
1479-6813
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1530/JME-14-0063
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
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dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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