MMP/TIMP balance is modulated in vitro by 15dPGJ2 in fetuses and placentas from diabetic rats

Autores
Pustovrh, María Carolina; Capobianco, Evangelina Lorena; Martinez, Nora Alicia; Higa, Romina Daniela; White, Verónica; Jawerbaum, Alicia Sandra
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Maternal diabetes is associated with morphological placental abnormalities and foeto-placental impairments. These alterations are linked with a dysregulation of the activity of matrix metalloproteinases (MMPs). We investigated the action of 15deoxyD12,14 prostaglandin J2 (15dPGJ2), a natural ligand of the peroxisome proliferator activated receptor (PPAR) gamma, on MMP-2 and MMP-9 activities and tissue inhibitors of matrix metalloproteinases (TIMP) levels in foetuses and placentas from diabetic rats. Materials and methods: Diabetes was induced in rat neonates by a single streptozotocin administration (90 mg kg)1 s.c.). At 13Æ5 days of gestation, foetal and placental homogenates were prepared for the determination of PPARgamma levels (western blot) and 15dPGJ2 concentration (enzyme-immunoassay), whereas the in vitro effect of 15dPGJ2 (2 uM) was evaluated on placental and foetal MMPs and TIMP activities (zymography and reverse zymography), nitrate ⁄ nitrite concentrations (Griess method) and thiobarbituric acid reactive substances (TBARS). Results: PPARgamma was increased while 15dPGJ2 was decreased in placentas and foetuses from diabetic rats. 15dPGJ2 additions were able to reduce the high activities of MMP-2 and MMP-9 present in diabetic placental tissues. 15dPGJ2 additions reduced MMP-2 activity in control and diabetic foetuses. TIMP-3 levels were decreased in diabetic placentas and 15dPGJ2 was able to enhance them to control values. Nitrates ⁄ nitrites and TBARS, metabolites of MMPs activators, were increased in the diabetic placenta and reduced by 15dPGJ2. Conclusions: This study demonstrates that 15dPGJ2 is a potent modulator of the balance between MMP activities and TIMP levels, which is needed in the correct formation and function of the placenta and foetal organs.
Fil: Pustovrh, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Capobianco, Evangelina Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Martinez, Nora Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Higa, Romina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: White, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Jawerbaum, Alicia Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Materia
15DPGJ2
DIABETES
FETUS
MMPS
PLACENTA
PPARΓ
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/123034
Acceder
id CONICETDig_5efa8cfd02e5f0324415261be931248c
oai_identifier_str oai:ri.conicet.gov.ar:11336/123034
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling MMP/TIMP balance is modulated in vitro by 15dPGJ2 in fetuses and placentas from diabetic ratsPustovrh, María CarolinaCapobianco, Evangelina LorenaMartinez, Nora AliciaHiga, Romina DanielaWhite, VerónicaJawerbaum, Alicia Sandra15DPGJ2DIABETESFETUSMMPSPLACENTAPPARΓhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background: Maternal diabetes is associated with morphological placental abnormalities and foeto-placental impairments. These alterations are linked with a dysregulation of the activity of matrix metalloproteinases (MMPs). We investigated the action of 15deoxyD12,14 prostaglandin J2 (15dPGJ2), a natural ligand of the peroxisome proliferator activated receptor (PPAR) gamma, on MMP-2 and MMP-9 activities and tissue inhibitors of matrix metalloproteinases (TIMP) levels in foetuses and placentas from diabetic rats. Materials and methods: Diabetes was induced in rat neonates by a single streptozotocin administration (90 mg kg)1 s.c.). At 13Æ5 days of gestation, foetal and placental homogenates were prepared for the determination of PPARgamma levels (western blot) and 15dPGJ2 concentration (enzyme-immunoassay), whereas the in vitro effect of 15dPGJ2 (2 uM) was evaluated on placental and foetal MMPs and TIMP activities (zymography and reverse zymography), nitrate ⁄ nitrite concentrations (Griess method) and thiobarbituric acid reactive substances (TBARS). Results: PPARgamma was increased while 15dPGJ2 was decreased in placentas and foetuses from diabetic rats. 15dPGJ2 additions were able to reduce the high activities of MMP-2 and MMP-9 present in diabetic placental tissues. 15dPGJ2 additions reduced MMP-2 activity in control and diabetic foetuses. TIMP-3 levels were decreased in diabetic placentas and 15dPGJ2 was able to enhance them to control values. Nitrates ⁄ nitrites and TBARS, metabolites of MMPs activators, were increased in the diabetic placenta and reduced by 15dPGJ2. Conclusions: This study demonstrates that 15dPGJ2 is a potent modulator of the balance between MMP activities and TIMP levels, which is needed in the correct formation and function of the placenta and foetal organs.Fil: Pustovrh, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Capobianco, Evangelina Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Martinez, Nora Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Higa, Romina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: White, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Jawerbaum, Alicia Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaWiley Blackwell Publishing, Inc2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/123034Pustovrh, María Carolina; Capobianco, Evangelina Lorena; Martinez, Nora Alicia; Higa, Romina Daniela; White, Verónica; et al.; MMP/TIMP balance is modulated in vitro by 15dPGJ2 in fetuses and placentas from diabetic rats; Wiley Blackwell Publishing, Inc; European Journal of Clinical Investigation; 39; 12; 12-2009; 1082-10900014-2972CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2362.2009.02200.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2362.2009.02200.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:36:57Zoai:ri.conicet.gov.ar:11336/123034instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:36:58.107CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv MMP/TIMP balance is modulated in vitro by 15dPGJ2 in fetuses and placentas from diabetic rats
title MMP/TIMP balance is modulated in vitro by 15dPGJ2 in fetuses and placentas from diabetic rats
spellingShingle MMP/TIMP balance is modulated in vitro by 15dPGJ2 in fetuses and placentas from diabetic rats
Pustovrh, María Carolina
15DPGJ2
DIABETES
FETUS
MMPS
PLACENTA
PPARΓ
title_short MMP/TIMP balance is modulated in vitro by 15dPGJ2 in fetuses and placentas from diabetic rats
title_full MMP/TIMP balance is modulated in vitro by 15dPGJ2 in fetuses and placentas from diabetic rats
title_fullStr MMP/TIMP balance is modulated in vitro by 15dPGJ2 in fetuses and placentas from diabetic rats
title_full_unstemmed MMP/TIMP balance is modulated in vitro by 15dPGJ2 in fetuses and placentas from diabetic rats
title_sort MMP/TIMP balance is modulated in vitro by 15dPGJ2 in fetuses and placentas from diabetic rats
dc.creator.none.fl_str_mv Pustovrh, María Carolina
Capobianco, Evangelina Lorena
Martinez, Nora Alicia
Higa, Romina Daniela
White, Verónica
Jawerbaum, Alicia Sandra
author Pustovrh, María Carolina
author_facet Pustovrh, María Carolina
Capobianco, Evangelina Lorena
Martinez, Nora Alicia
Higa, Romina Daniela
White, Verónica
Jawerbaum, Alicia Sandra
author_role author
author2 Capobianco, Evangelina Lorena
Martinez, Nora Alicia
Higa, Romina Daniela
White, Verónica
Jawerbaum, Alicia Sandra
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv 15DPGJ2
DIABETES
FETUS
MMPS
PLACENTA
PPARΓ
topic 15DPGJ2
DIABETES
FETUS
MMPS
PLACENTA
PPARΓ
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Maternal diabetes is associated with morphological placental abnormalities and foeto-placental impairments. These alterations are linked with a dysregulation of the activity of matrix metalloproteinases (MMPs). We investigated the action of 15deoxyD12,14 prostaglandin J2 (15dPGJ2), a natural ligand of the peroxisome proliferator activated receptor (PPAR) gamma, on MMP-2 and MMP-9 activities and tissue inhibitors of matrix metalloproteinases (TIMP) levels in foetuses and placentas from diabetic rats. Materials and methods: Diabetes was induced in rat neonates by a single streptozotocin administration (90 mg kg)1 s.c.). At 13Æ5 days of gestation, foetal and placental homogenates were prepared for the determination of PPARgamma levels (western blot) and 15dPGJ2 concentration (enzyme-immunoassay), whereas the in vitro effect of 15dPGJ2 (2 uM) was evaluated on placental and foetal MMPs and TIMP activities (zymography and reverse zymography), nitrate ⁄ nitrite concentrations (Griess method) and thiobarbituric acid reactive substances (TBARS). Results: PPARgamma was increased while 15dPGJ2 was decreased in placentas and foetuses from diabetic rats. 15dPGJ2 additions were able to reduce the high activities of MMP-2 and MMP-9 present in diabetic placental tissues. 15dPGJ2 additions reduced MMP-2 activity in control and diabetic foetuses. TIMP-3 levels were decreased in diabetic placentas and 15dPGJ2 was able to enhance them to control values. Nitrates ⁄ nitrites and TBARS, metabolites of MMPs activators, were increased in the diabetic placenta and reduced by 15dPGJ2. Conclusions: This study demonstrates that 15dPGJ2 is a potent modulator of the balance between MMP activities and TIMP levels, which is needed in the correct formation and function of the placenta and foetal organs.
Fil: Pustovrh, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Capobianco, Evangelina Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Martinez, Nora Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Higa, Romina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: White, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Jawerbaum, Alicia Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
description Background: Maternal diabetes is associated with morphological placental abnormalities and foeto-placental impairments. These alterations are linked with a dysregulation of the activity of matrix metalloproteinases (MMPs). We investigated the action of 15deoxyD12,14 prostaglandin J2 (15dPGJ2), a natural ligand of the peroxisome proliferator activated receptor (PPAR) gamma, on MMP-2 and MMP-9 activities and tissue inhibitors of matrix metalloproteinases (TIMP) levels in foetuses and placentas from diabetic rats. Materials and methods: Diabetes was induced in rat neonates by a single streptozotocin administration (90 mg kg)1 s.c.). At 13Æ5 days of gestation, foetal and placental homogenates were prepared for the determination of PPARgamma levels (western blot) and 15dPGJ2 concentration (enzyme-immunoassay), whereas the in vitro effect of 15dPGJ2 (2 uM) was evaluated on placental and foetal MMPs and TIMP activities (zymography and reverse zymography), nitrate ⁄ nitrite concentrations (Griess method) and thiobarbituric acid reactive substances (TBARS). Results: PPARgamma was increased while 15dPGJ2 was decreased in placentas and foetuses from diabetic rats. 15dPGJ2 additions were able to reduce the high activities of MMP-2 and MMP-9 present in diabetic placental tissues. 15dPGJ2 additions reduced MMP-2 activity in control and diabetic foetuses. TIMP-3 levels were decreased in diabetic placentas and 15dPGJ2 was able to enhance them to control values. Nitrates ⁄ nitrites and TBARS, metabolites of MMPs activators, were increased in the diabetic placenta and reduced by 15dPGJ2. Conclusions: This study demonstrates that 15dPGJ2 is a potent modulator of the balance between MMP activities and TIMP levels, which is needed in the correct formation and function of the placenta and foetal organs.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/123034
Pustovrh, María Carolina; Capobianco, Evangelina Lorena; Martinez, Nora Alicia; Higa, Romina Daniela; White, Verónica; et al.; MMP/TIMP balance is modulated in vitro by 15dPGJ2 in fetuses and placentas from diabetic rats; Wiley Blackwell Publishing, Inc; European Journal of Clinical Investigation; 39; 12; 12-2009; 1082-1090
0014-2972
CONICET Digital
CONICET
url http://hdl.handle.net/11336/123034
identifier_str_mv Pustovrh, María Carolina; Capobianco, Evangelina Lorena; Martinez, Nora Alicia; Higa, Romina Daniela; White, Verónica; et al.; MMP/TIMP balance is modulated in vitro by 15dPGJ2 in fetuses and placentas from diabetic rats; Wiley Blackwell Publishing, Inc; European Journal of Clinical Investigation; 39; 12; 12-2009; 1082-1090
0014-2972
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2362.2009.02200.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.121305

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