Evaluation of zearalenone, α-zearalenol, β-zearalenol, zearalenone 4-sulfate and β-zearalenol 4-glucoside levels during the ensiling process

Autores
Gonzalez Pereyra, Maria Laura; Sulyok, M.; Baralla, V.; Dalcero, Ana Maria; Krska, R.; Chulze, Sofia Noemi; Cavaglieri, Lilia Reneé
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Zearalenone-producing Fusarium species can contaminate maize before ensiling and cause reproductive problems in animals. Suspect feeds are only routinely analysed for zearalenone (ZEA), not considering other oestrogenic metabolites or masked derivatives. The aims of the present study were to monitor the levels of ZEA, α-zearalenol (α-ZOL), β-zearalenol (β-ZOL), zearalenone-4-sulfate (ZEA-4S) and β-zearalenol-4-glucoside (β-ZOL-4G) in artificially contaminated maize silage and determine the effect of the ensiling process on these toxins. A laboratory silo model was designed using polystyrene bags filled with previously contaminated chopped whole-plant maize, stored in a dry and cool room and sampled at days 7, 45, 90, 120 and 127. ZEA, α-ZOL, β-ZOL, ZEA-4S and β-ZOL-4G levels were quantified by liquid chromatography – tandem mass spectrometry. Chemical and physical analysis indicated silage maintained good quality in all stages. pH was reduced favourably (P<0.05) from 4.69 to 3.80 during the preservation stage. Dry matter, moisture content and water activity did not vary from day 7 to 127. ZEA, α-ZOL, β-ZOL and ZEA-4S levels also did not change from day 7 to 127, indicating no significant degradation by the ensiling process or silage-native microbiota. This study suggests that ZEA levels remain invariable during the ensiling process, as well as the levels of its derivatives. The presence of highly oestrogenic metabolites, like α-ZOL and the masked ZEA-4S, which are not screened in the routine analyses, increases the overall toxicity of ZEA-contaminated silage.
Fil: Gonzalez Pereyra, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Sulyok, M.. University of Natural Resources and Life Science. Viena; Suiza
Fil: Baralla, V.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Dalcero, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Krska, R.. University of Natural Resources and Life Science. Viena; Suiza
Fil: Chulze, Sofia Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Cavaglieri, Lilia Reneé. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Materia
Α-Zearalenol
Β-Zearalenol
Masked Mycotoxins
Silage
Zearalenone
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33810
Acceder
id CONICETDig_59247ed2cb22d14bb1674daa20af8e25
oai_identifier_str oai:ri.conicet.gov.ar:11336/33810
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of zearalenone, α-zearalenol, β-zearalenol, zearalenone 4-sulfate and β-zearalenol 4-glucoside levels during the ensiling processGonzalez Pereyra, Maria LauraSulyok, M.Baralla, V.Dalcero, Ana MariaKrska, R.Chulze, Sofia NoemiCavaglieri, Lilia ReneéΑ-ZearalenolΒ-ZearalenolMasked MycotoxinsSilageZearalenonehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Zearalenone-producing Fusarium species can contaminate maize before ensiling and cause reproductive problems in animals. Suspect feeds are only routinely analysed for zearalenone (ZEA), not considering other oestrogenic metabolites or masked derivatives. The aims of the present study were to monitor the levels of ZEA, α-zearalenol (α-ZOL), β-zearalenol (β-ZOL), zearalenone-4-sulfate (ZEA-4S) and β-zearalenol-4-glucoside (β-ZOL-4G) in artificially contaminated maize silage and determine the effect of the ensiling process on these toxins. A laboratory silo model was designed using polystyrene bags filled with previously contaminated chopped whole-plant maize, stored in a dry and cool room and sampled at days 7, 45, 90, 120 and 127. ZEA, α-ZOL, β-ZOL, ZEA-4S and β-ZOL-4G levels were quantified by liquid chromatography – tandem mass spectrometry. Chemical and physical analysis indicated silage maintained good quality in all stages. pH was reduced favourably (P<0.05) from 4.69 to 3.80 during the preservation stage. Dry matter, moisture content and water activity did not vary from day 7 to 127. ZEA, α-ZOL, β-ZOL and ZEA-4S levels also did not change from day 7 to 127, indicating no significant degradation by the ensiling process or silage-native microbiota. This study suggests that ZEA levels remain invariable during the ensiling process, as well as the levels of its derivatives. The presence of highly oestrogenic metabolites, like α-ZOL and the masked ZEA-4S, which are not screened in the routine analyses, increases the overall toxicity of ZEA-contaminated silage.Fil: Gonzalez Pereyra, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Sulyok, M.. University of Natural Resources and Life Science. Viena; SuizaFil: Baralla, V.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Dalcero, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Krska, R.. University of Natural Resources and Life Science. Viena; SuizaFil: Chulze, Sofia Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Cavaglieri, Lilia Reneé. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaWageningen Academic Publishers2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33810Gonzalez Pereyra, Maria Laura; Sulyok, M.; Baralla, V.; Dalcero, Ana Maria; Krska, R.; et al.; Evaluation of zearalenone, α-zearalenol, β-zearalenol, zearalenone 4-sulfate and β-zearalenol 4-glucoside levels during the ensiling process; Wageningen Academic Publishers; World Mycotoxin Journal; 7; 3; 3-2014; 291-2951875-0710CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.wageningenacademic.com/doi/10.3920/WMJ2013.1638info:eu-repo/semantics/altIdentifier/doi/10.3920/WMJ2013.1638info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:03Zoai:ri.conicet.gov.ar:11336/33810instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:03.625CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of zearalenone, α-zearalenol, β-zearalenol, zearalenone 4-sulfate and β-zearalenol 4-glucoside levels during the ensiling process
title Evaluation of zearalenone, α-zearalenol, β-zearalenol, zearalenone 4-sulfate and β-zearalenol 4-glucoside levels during the ensiling process
spellingShingle Evaluation of zearalenone, α-zearalenol, β-zearalenol, zearalenone 4-sulfate and β-zearalenol 4-glucoside levels during the ensiling process
Gonzalez Pereyra, Maria Laura
Α-Zearalenol
Β-Zearalenol
Masked Mycotoxins
Silage
Zearalenone
title_short Evaluation of zearalenone, α-zearalenol, β-zearalenol, zearalenone 4-sulfate and β-zearalenol 4-glucoside levels during the ensiling process
title_full Evaluation of zearalenone, α-zearalenol, β-zearalenol, zearalenone 4-sulfate and β-zearalenol 4-glucoside levels during the ensiling process
title_fullStr Evaluation of zearalenone, α-zearalenol, β-zearalenol, zearalenone 4-sulfate and β-zearalenol 4-glucoside levels during the ensiling process
title_full_unstemmed Evaluation of zearalenone, α-zearalenol, β-zearalenol, zearalenone 4-sulfate and β-zearalenol 4-glucoside levels during the ensiling process
title_sort Evaluation of zearalenone, α-zearalenol, β-zearalenol, zearalenone 4-sulfate and β-zearalenol 4-glucoside levels during the ensiling process
dc.creator.none.fl_str_mv Gonzalez Pereyra, Maria Laura
Sulyok, M.
Baralla, V.
Dalcero, Ana Maria
Krska, R.
Chulze, Sofia Noemi
Cavaglieri, Lilia Reneé
author Gonzalez Pereyra, Maria Laura
author_facet Gonzalez Pereyra, Maria Laura
Sulyok, M.
Baralla, V.
Dalcero, Ana Maria
Krska, R.
Chulze, Sofia Noemi
Cavaglieri, Lilia Reneé
author_role author
author2 Sulyok, M.
Baralla, V.
Dalcero, Ana Maria
Krska, R.
Chulze, Sofia Noemi
Cavaglieri, Lilia Reneé
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Α-Zearalenol
Β-Zearalenol
Masked Mycotoxins
Silage
Zearalenone
topic Α-Zearalenol
Β-Zearalenol
Masked Mycotoxins
Silage
Zearalenone
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Zearalenone-producing Fusarium species can contaminate maize before ensiling and cause reproductive problems in animals. Suspect feeds are only routinely analysed for zearalenone (ZEA), not considering other oestrogenic metabolites or masked derivatives. The aims of the present study were to monitor the levels of ZEA, α-zearalenol (α-ZOL), β-zearalenol (β-ZOL), zearalenone-4-sulfate (ZEA-4S) and β-zearalenol-4-glucoside (β-ZOL-4G) in artificially contaminated maize silage and determine the effect of the ensiling process on these toxins. A laboratory silo model was designed using polystyrene bags filled with previously contaminated chopped whole-plant maize, stored in a dry and cool room and sampled at days 7, 45, 90, 120 and 127. ZEA, α-ZOL, β-ZOL, ZEA-4S and β-ZOL-4G levels were quantified by liquid chromatography – tandem mass spectrometry. Chemical and physical analysis indicated silage maintained good quality in all stages. pH was reduced favourably (P<0.05) from 4.69 to 3.80 during the preservation stage. Dry matter, moisture content and water activity did not vary from day 7 to 127. ZEA, α-ZOL, β-ZOL and ZEA-4S levels also did not change from day 7 to 127, indicating no significant degradation by the ensiling process or silage-native microbiota. This study suggests that ZEA levels remain invariable during the ensiling process, as well as the levels of its derivatives. The presence of highly oestrogenic metabolites, like α-ZOL and the masked ZEA-4S, which are not screened in the routine analyses, increases the overall toxicity of ZEA-contaminated silage.
Fil: Gonzalez Pereyra, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Sulyok, M.. University of Natural Resources and Life Science. Viena; Suiza
Fil: Baralla, V.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Dalcero, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Krska, R.. University of Natural Resources and Life Science. Viena; Suiza
Fil: Chulze, Sofia Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Cavaglieri, Lilia Reneé. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
description Zearalenone-producing Fusarium species can contaminate maize before ensiling and cause reproductive problems in animals. Suspect feeds are only routinely analysed for zearalenone (ZEA), not considering other oestrogenic metabolites or masked derivatives. The aims of the present study were to monitor the levels of ZEA, α-zearalenol (α-ZOL), β-zearalenol (β-ZOL), zearalenone-4-sulfate (ZEA-4S) and β-zearalenol-4-glucoside (β-ZOL-4G) in artificially contaminated maize silage and determine the effect of the ensiling process on these toxins. A laboratory silo model was designed using polystyrene bags filled with previously contaminated chopped whole-plant maize, stored in a dry and cool room and sampled at days 7, 45, 90, 120 and 127. ZEA, α-ZOL, β-ZOL, ZEA-4S and β-ZOL-4G levels were quantified by liquid chromatography – tandem mass spectrometry. Chemical and physical analysis indicated silage maintained good quality in all stages. pH was reduced favourably (P<0.05) from 4.69 to 3.80 during the preservation stage. Dry matter, moisture content and water activity did not vary from day 7 to 127. ZEA, α-ZOL, β-ZOL and ZEA-4S levels also did not change from day 7 to 127, indicating no significant degradation by the ensiling process or silage-native microbiota. This study suggests that ZEA levels remain invariable during the ensiling process, as well as the levels of its derivatives. The presence of highly oestrogenic metabolites, like α-ZOL and the masked ZEA-4S, which are not screened in the routine analyses, increases the overall toxicity of ZEA-contaminated silage.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33810
Gonzalez Pereyra, Maria Laura; Sulyok, M.; Baralla, V.; Dalcero, Ana Maria; Krska, R.; et al.; Evaluation of zearalenone, α-zearalenol, β-zearalenol, zearalenone 4-sulfate and β-zearalenol 4-glucoside levels during the ensiling process; Wageningen Academic Publishers; World Mycotoxin Journal; 7; 3; 3-2014; 291-295
1875-0710
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33810
identifier_str_mv Gonzalez Pereyra, Maria Laura; Sulyok, M.; Baralla, V.; Dalcero, Ana Maria; Krska, R.; et al.; Evaluation of zearalenone, α-zearalenol, β-zearalenol, zearalenone 4-sulfate and β-zearalenol 4-glucoside levels during the ensiling process; Wageningen Academic Publishers; World Mycotoxin Journal; 7; 3; 3-2014; 291-295
1875-0710
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.wageningenacademic.com/doi/10.3920/WMJ2013.1638
info:eu-repo/semantics/altIdentifier/doi/10.3920/WMJ2013.1638
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wageningen Academic Publishers
publisher.none.fl_str_mv Wageningen Academic Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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