First detection of PCV4 in swine in the United States: codetection with PCV2 and PCV3 and direct detection within tissues

Autores
Kroeger, Molly; Vargas Bermudez, Diana S.; Jaime, Jairo; Parada, Julian; Groeltz, Jennifer; Gauger, Philip; Piñeyro, Pablo
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Since PCV4 was first described in 2019, the virus has been identified in several countries in SoutheastAsia and Europe. Most studies have been limited to detecting PCV4 by PCR. Thus, PCV4 has an unclearassociation with clinical disease. This study utilized 512 porcine clinical lung, feces, spleen, serum,lymphoid tissue, and fetus samples submitted to the ISU‑VDL from June–September 2023. PCV4 wasdetected in 8.6% of samples with an average Ct value of 33. While detection rates among sample typeswere variable, lymphoid tissue had the highest detection rate (18.7%). Two ORF2 sequences wereobtained from lymphoid tissue samples and had 96.36–98.98% nucleotide identity with referencesequences. Direct detection of PCV4 by RNAscope revealed viral replication in B lymphocytes andmacrophages in lymph node germinal centers and histiocytic and T lymphocyte infiltration in thelamina propria of the small intestine. PCV4 detection was most commonly observed in nursery tofinishing aged pigs displaying respiratory and enteric disease. Coinfection with PCV2, PCV3, and otherendemic pathogens was frequently observed, highlighting the complex interplay between differentPCVs and their potential roles in disease pathogenesis. This study provides insights into the frequencyof detection, tissue distribution, and genetic characteristics of PCV4 in the US.
Fil: Kroeger, Molly. University of Iowa; Estados Unidos
Fil: Vargas Bermudez, Diana S.. Universidad Nacional de Colombia. Sede Bogotá; Colombia
Fil: Jaime, Jairo. Universidad Nacional de Colombia. Sede Bogotá; Colombia
Fil: Parada, Julian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Groeltz, Jennifer. University of Iowa; Estados Unidos
Fil: Gauger, Philip. University of Iowa; Estados Unidos
Fil: Piñeyro, Pablo. University of Iowa; Estados Unidos
Materia
PCV4
In situ hybridization
Lymphoid tissue
Coinfection,
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/240152
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling First detection of PCV4 in swine in the United States: codetection with PCV2 and PCV3 and direct detection within tissuesKroeger, MollyVargas Bermudez, Diana S.Jaime, JairoParada, JulianGroeltz, JenniferGauger, PhilipPiñeyro, PabloPCV4In situ hybridizationLymphoid tissueCoinfection,https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Since PCV4 was first described in 2019, the virus has been identified in several countries in SoutheastAsia and Europe. Most studies have been limited to detecting PCV4 by PCR. Thus, PCV4 has an unclearassociation with clinical disease. This study utilized 512 porcine clinical lung, feces, spleen, serum,lymphoid tissue, and fetus samples submitted to the ISU‑VDL from June–September 2023. PCV4 wasdetected in 8.6% of samples with an average Ct value of 33. While detection rates among sample typeswere variable, lymphoid tissue had the highest detection rate (18.7%). Two ORF2 sequences wereobtained from lymphoid tissue samples and had 96.36–98.98% nucleotide identity with referencesequences. Direct detection of PCV4 by RNAscope revealed viral replication in B lymphocytes andmacrophages in lymph node germinal centers and histiocytic and T lymphocyte infiltration in thelamina propria of the small intestine. PCV4 detection was most commonly observed in nursery tofinishing aged pigs displaying respiratory and enteric disease. Coinfection with PCV2, PCV3, and otherendemic pathogens was frequently observed, highlighting the complex interplay between differentPCVs and their potential roles in disease pathogenesis. This study provides insights into the frequencyof detection, tissue distribution, and genetic characteristics of PCV4 in the US.Fil: Kroeger, Molly. University of Iowa; Estados UnidosFil: Vargas Bermudez, Diana S.. Universidad Nacional de Colombia. Sede Bogotá; ColombiaFil: Jaime, Jairo. Universidad Nacional de Colombia. Sede Bogotá; ColombiaFil: Parada, Julian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Groeltz, Jennifer. University of Iowa; Estados UnidosFil: Gauger, Philip. University of Iowa; Estados UnidosFil: Piñeyro, Pablo. University of Iowa; Estados UnidosNature2024-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/240152Kroeger, Molly; Vargas Bermudez, Diana S.; Jaime, Jairo; Parada, Julian; Groeltz, Jennifer; et al.; First detection of PCV4 in swine in the United States: codetection with PCV2 and PCV3 and direct detection within tissues; Nature; Scientific Reports; 14; 1; 7-2024; 1-142045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-024-66328-yinfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-024-66328-yinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:31Zoai:ri.conicet.gov.ar:11336/240152instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:31.382CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First detection of PCV4 in swine in the United States: codetection with PCV2 and PCV3 and direct detection within tissues
title First detection of PCV4 in swine in the United States: codetection with PCV2 and PCV3 and direct detection within tissues
spellingShingle First detection of PCV4 in swine in the United States: codetection with PCV2 and PCV3 and direct detection within tissues
Kroeger, Molly
PCV4
In situ hybridization
Lymphoid tissue
Coinfection,
title_short First detection of PCV4 in swine in the United States: codetection with PCV2 and PCV3 and direct detection within tissues
title_full First detection of PCV4 in swine in the United States: codetection with PCV2 and PCV3 and direct detection within tissues
title_fullStr First detection of PCV4 in swine in the United States: codetection with PCV2 and PCV3 and direct detection within tissues
title_full_unstemmed First detection of PCV4 in swine in the United States: codetection with PCV2 and PCV3 and direct detection within tissues
title_sort First detection of PCV4 in swine in the United States: codetection with PCV2 and PCV3 and direct detection within tissues
dc.creator.none.fl_str_mv Kroeger, Molly
Vargas Bermudez, Diana S.
Jaime, Jairo
Parada, Julian
Groeltz, Jennifer
Gauger, Philip
Piñeyro, Pablo
author Kroeger, Molly
author_facet Kroeger, Molly
Vargas Bermudez, Diana S.
Jaime, Jairo
Parada, Julian
Groeltz, Jennifer
Gauger, Philip
Piñeyro, Pablo
author_role author
author2 Vargas Bermudez, Diana S.
Jaime, Jairo
Parada, Julian
Groeltz, Jennifer
Gauger, Philip
Piñeyro, Pablo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv PCV4
In situ hybridization
Lymphoid tissue
Coinfection,
topic PCV4
In situ hybridization
Lymphoid tissue
Coinfection,
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Since PCV4 was first described in 2019, the virus has been identified in several countries in SoutheastAsia and Europe. Most studies have been limited to detecting PCV4 by PCR. Thus, PCV4 has an unclearassociation with clinical disease. This study utilized 512 porcine clinical lung, feces, spleen, serum,lymphoid tissue, and fetus samples submitted to the ISU‑VDL from June–September 2023. PCV4 wasdetected in 8.6% of samples with an average Ct value of 33. While detection rates among sample typeswere variable, lymphoid tissue had the highest detection rate (18.7%). Two ORF2 sequences wereobtained from lymphoid tissue samples and had 96.36–98.98% nucleotide identity with referencesequences. Direct detection of PCV4 by RNAscope revealed viral replication in B lymphocytes andmacrophages in lymph node germinal centers and histiocytic and T lymphocyte infiltration in thelamina propria of the small intestine. PCV4 detection was most commonly observed in nursery tofinishing aged pigs displaying respiratory and enteric disease. Coinfection with PCV2, PCV3, and otherendemic pathogens was frequently observed, highlighting the complex interplay between differentPCVs and their potential roles in disease pathogenesis. This study provides insights into the frequencyof detection, tissue distribution, and genetic characteristics of PCV4 in the US.
Fil: Kroeger, Molly. University of Iowa; Estados Unidos
Fil: Vargas Bermudez, Diana S.. Universidad Nacional de Colombia. Sede Bogotá; Colombia
Fil: Jaime, Jairo. Universidad Nacional de Colombia. Sede Bogotá; Colombia
Fil: Parada, Julian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Groeltz, Jennifer. University of Iowa; Estados Unidos
Fil: Gauger, Philip. University of Iowa; Estados Unidos
Fil: Piñeyro, Pablo. University of Iowa; Estados Unidos
description Since PCV4 was first described in 2019, the virus has been identified in several countries in SoutheastAsia and Europe. Most studies have been limited to detecting PCV4 by PCR. Thus, PCV4 has an unclearassociation with clinical disease. This study utilized 512 porcine clinical lung, feces, spleen, serum,lymphoid tissue, and fetus samples submitted to the ISU‑VDL from June–September 2023. PCV4 wasdetected in 8.6% of samples with an average Ct value of 33. While detection rates among sample typeswere variable, lymphoid tissue had the highest detection rate (18.7%). Two ORF2 sequences wereobtained from lymphoid tissue samples and had 96.36–98.98% nucleotide identity with referencesequences. Direct detection of PCV4 by RNAscope revealed viral replication in B lymphocytes andmacrophages in lymph node germinal centers and histiocytic and T lymphocyte infiltration in thelamina propria of the small intestine. PCV4 detection was most commonly observed in nursery tofinishing aged pigs displaying respiratory and enteric disease. Coinfection with PCV2, PCV3, and otherendemic pathogens was frequently observed, highlighting the complex interplay between differentPCVs and their potential roles in disease pathogenesis. This study provides insights into the frequencyof detection, tissue distribution, and genetic characteristics of PCV4 in the US.
publishDate 2024
dc.date.none.fl_str_mv 2024-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/240152
Kroeger, Molly; Vargas Bermudez, Diana S.; Jaime, Jairo; Parada, Julian; Groeltz, Jennifer; et al.; First detection of PCV4 in swine in the United States: codetection with PCV2 and PCV3 and direct detection within tissues; Nature; Scientific Reports; 14; 1; 7-2024; 1-14
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/240152
identifier_str_mv Kroeger, Molly; Vargas Bermudez, Diana S.; Jaime, Jairo; Parada, Julian; Groeltz, Jennifer; et al.; First detection of PCV4 in swine in the United States: codetection with PCV2 and PCV3 and direct detection within tissues; Nature; Scientific Reports; 14; 1; 7-2024; 1-14
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-024-66328-y
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-024-66328-y
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature
publisher.none.fl_str_mv Nature
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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