Isolation and characterization of bovine Limbal mesenchymal stromal cells for application in ocular toxicity studies

Autores
Benedetti, Martina Daniela; Lenze, Mariela; Garcia, Mora Reneé; Magaña Guerrero, Sofía Fátima; Wikinski, Silvia Ines; Garfias, Yonathan; Gutiérrez, María Laura
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Draize test in rabbits has historically been the regulatory standard, it presents significant ethical and scientific limitations. While validated in vitro methods are currently available, most of them do not allow the evaluation of damage reversibility—a key parameter for hazard classification under the United Nations Globally Harmonized System (UN GHS).This study evaluates the utility of an in vitro model based on limbal mesenchymal stromal cells (LMSC) for ocular toxicity testing and the prediction of corneal damage reversibility. LMSC were obtained from slaughterhouse-derived residual tissue, cultured under specific conditions, and briefly exposed to substances classified according to UN GHS. Cell migration was then assessed as an indicator of tissue recovery.This preliminary report presents, to our knowledge, the first application of short-term (5-min) exposure in a scratch assay to evaluate its effect on migration capacity. Unlike previous studies relying on prolonged exposure to inhibitory compounds, this approach better simulates accidental ocular exposure scenarios. While migration was not significantly disrupted under the tested conditions, these results offer valuable insight into the relationship between viability and wound healing and highlight the importance of exposure time and concentration in in vitro ocular toxicity models. Overall, this work establishes a promising foundation for future refinement of alternative methods that aim to predict damage persistence and reversibility more accurately.
Fil: Benedetti, Martina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; Argentina
Fil: Lenze, Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; Argentina
Fil: Garcia, Mora Reneé. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Magaña Guerrero, Sofía Fátima. Universidad Nacional Autónoma de México; México
Fil: Wikinski, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Garfias, Yonathan. Universidad Nacional Autónoma de México; México
Fil: Gutiérrez, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
3R
CELULAS MADRE
REGENERACION CORNEAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/281825
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/281825
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isolation and characterization of bovine Limbal mesenchymal stromal cells for application in ocular toxicity studiesBenedetti, Martina DanielaLenze, MarielaGarcia, Mora ReneéMagaña Guerrero, Sofía FátimaWikinski, Silvia InesGarfias, YonathanGutiérrez, María Laura3RCELULAS MADREREGENERACION CORNEALhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Draize test in rabbits has historically been the regulatory standard, it presents significant ethical and scientific limitations. While validated in vitro methods are currently available, most of them do not allow the evaluation of damage reversibility—a key parameter for hazard classification under the United Nations Globally Harmonized System (UN GHS).This study evaluates the utility of an in vitro model based on limbal mesenchymal stromal cells (LMSC) for ocular toxicity testing and the prediction of corneal damage reversibility. LMSC were obtained from slaughterhouse-derived residual tissue, cultured under specific conditions, and briefly exposed to substances classified according to UN GHS. Cell migration was then assessed as an indicator of tissue recovery.This preliminary report presents, to our knowledge, the first application of short-term (5-min) exposure in a scratch assay to evaluate its effect on migration capacity. Unlike previous studies relying on prolonged exposure to inhibitory compounds, this approach better simulates accidental ocular exposure scenarios. While migration was not significantly disrupted under the tested conditions, these results offer valuable insight into the relationship between viability and wound healing and highlight the importance of exposure time and concentration in in vitro ocular toxicity models. Overall, this work establishes a promising foundation for future refinement of alternative methods that aim to predict damage persistence and reversibility more accurately.Fil: Benedetti, Martina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; ArgentinaFil: Lenze, Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; ArgentinaFil: Garcia, Mora Reneé. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Magaña Guerrero, Sofía Fátima. Universidad Nacional Autónoma de México; MéxicoFil: Wikinski, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Garfias, Yonathan. Universidad Nacional Autónoma de México; MéxicoFil: Gutiérrez, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaPergamon-Elsevier Science Ltd2025-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/281825Benedetti, Martina Daniela; Lenze, Mariela; Garcia, Mora Reneé; Magaña Guerrero, Sofía Fátima; Wikinski, Silvia Ines; et al.; Isolation and characterization of bovine Limbal mesenchymal stromal cells for application in ocular toxicity studies; Pergamon-Elsevier Science Ltd; Toxicology in Vitro; 109; 12-20250887-2333CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0887233325001195info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tiv.2025.106125info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-03-31T15:28:02Zoai:ri.conicet.gov.ar:11336/281825instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-03-31 15:28:03.119CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation and characterization of bovine Limbal mesenchymal stromal cells for application in ocular toxicity studies
title Isolation and characterization of bovine Limbal mesenchymal stromal cells for application in ocular toxicity studies
spellingShingle Isolation and characterization of bovine Limbal mesenchymal stromal cells for application in ocular toxicity studies
Benedetti, Martina Daniela
3R
CELULAS MADRE
REGENERACION CORNEAL
title_short Isolation and characterization of bovine Limbal mesenchymal stromal cells for application in ocular toxicity studies
title_full Isolation and characterization of bovine Limbal mesenchymal stromal cells for application in ocular toxicity studies
title_fullStr Isolation and characterization of bovine Limbal mesenchymal stromal cells for application in ocular toxicity studies
title_full_unstemmed Isolation and characterization of bovine Limbal mesenchymal stromal cells for application in ocular toxicity studies
title_sort Isolation and characterization of bovine Limbal mesenchymal stromal cells for application in ocular toxicity studies
dc.creator.none.fl_str_mv Benedetti, Martina Daniela
Lenze, Mariela
Garcia, Mora Reneé
Magaña Guerrero, Sofía Fátima
Wikinski, Silvia Ines
Garfias, Yonathan
Gutiérrez, María Laura
author Benedetti, Martina Daniela
author_facet Benedetti, Martina Daniela
Lenze, Mariela
Garcia, Mora Reneé
Magaña Guerrero, Sofía Fátima
Wikinski, Silvia Ines
Garfias, Yonathan
Gutiérrez, María Laura
author_role author
author2 Lenze, Mariela
Garcia, Mora Reneé
Magaña Guerrero, Sofía Fátima
Wikinski, Silvia Ines
Garfias, Yonathan
Gutiérrez, María Laura
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv 3R
CELULAS MADRE
REGENERACION CORNEAL
topic 3R
CELULAS MADRE
REGENERACION CORNEAL
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Draize test in rabbits has historically been the regulatory standard, it presents significant ethical and scientific limitations. While validated in vitro methods are currently available, most of them do not allow the evaluation of damage reversibility—a key parameter for hazard classification under the United Nations Globally Harmonized System (UN GHS).This study evaluates the utility of an in vitro model based on limbal mesenchymal stromal cells (LMSC) for ocular toxicity testing and the prediction of corneal damage reversibility. LMSC were obtained from slaughterhouse-derived residual tissue, cultured under specific conditions, and briefly exposed to substances classified according to UN GHS. Cell migration was then assessed as an indicator of tissue recovery.This preliminary report presents, to our knowledge, the first application of short-term (5-min) exposure in a scratch assay to evaluate its effect on migration capacity. Unlike previous studies relying on prolonged exposure to inhibitory compounds, this approach better simulates accidental ocular exposure scenarios. While migration was not significantly disrupted under the tested conditions, these results offer valuable insight into the relationship between viability and wound healing and highlight the importance of exposure time and concentration in in vitro ocular toxicity models. Overall, this work establishes a promising foundation for future refinement of alternative methods that aim to predict damage persistence and reversibility more accurately.
Fil: Benedetti, Martina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; Argentina
Fil: Lenze, Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; Argentina
Fil: Garcia, Mora Reneé. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Magaña Guerrero, Sofía Fátima. Universidad Nacional Autónoma de México; México
Fil: Wikinski, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Garfias, Yonathan. Universidad Nacional Autónoma de México; México
Fil: Gutiérrez, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description Draize test in rabbits has historically been the regulatory standard, it presents significant ethical and scientific limitations. While validated in vitro methods are currently available, most of them do not allow the evaluation of damage reversibility—a key parameter for hazard classification under the United Nations Globally Harmonized System (UN GHS).This study evaluates the utility of an in vitro model based on limbal mesenchymal stromal cells (LMSC) for ocular toxicity testing and the prediction of corneal damage reversibility. LMSC were obtained from slaughterhouse-derived residual tissue, cultured under specific conditions, and briefly exposed to substances classified according to UN GHS. Cell migration was then assessed as an indicator of tissue recovery.This preliminary report presents, to our knowledge, the first application of short-term (5-min) exposure in a scratch assay to evaluate its effect on migration capacity. Unlike previous studies relying on prolonged exposure to inhibitory compounds, this approach better simulates accidental ocular exposure scenarios. While migration was not significantly disrupted under the tested conditions, these results offer valuable insight into the relationship between viability and wound healing and highlight the importance of exposure time and concentration in in vitro ocular toxicity models. Overall, this work establishes a promising foundation for future refinement of alternative methods that aim to predict damage persistence and reversibility more accurately.
publishDate 2025
dc.date.none.fl_str_mv 2025-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/281825
Benedetti, Martina Daniela; Lenze, Mariela; Garcia, Mora Reneé; Magaña Guerrero, Sofía Fátima; Wikinski, Silvia Ines; et al.; Isolation and characterization of bovine Limbal mesenchymal stromal cells for application in ocular toxicity studies; Pergamon-Elsevier Science Ltd; Toxicology in Vitro; 109; 12-2025
0887-2333
CONICET Digital
CONICET
url http://hdl.handle.net/11336/281825
identifier_str_mv Benedetti, Martina Daniela; Lenze, Mariela; Garcia, Mora Reneé; Magaña Guerrero, Sofía Fátima; Wikinski, Silvia Ines; et al.; Isolation and characterization of bovine Limbal mesenchymal stromal cells for application in ocular toxicity studies; Pergamon-Elsevier Science Ltd; Toxicology in Vitro; 109; 12-2025
0887-2333
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0887233325001195
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tiv.2025.106125
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.822162

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