Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soils

Autores
López, Ana María; Medina, Rocío; Bosch, María Alejandra Nieves; Yantorno, Osvaldo; Del Panno, María T.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Due to the reduced bioavailability that characterizes chronically hydrocarbons contaminated soils, the bioaugmentation with cultures obtained by enrichment in solid phase systems, could be a promissing strategy to apply in aged contaminated soils. In this context, the objective of this work was to study four bacterial component obtained from a degrader phenanthrene consortium by using solid phases with a resin XAD2 preloaded with phenanthrene, and to rebuild a defined degrading consortium from them. The isolated strains were identified by 16S rRNA and phenotypically characterized using the conventional assays. Furthermore, their main phenotypic features and biochemical characteristics where analyzed by Fourier-transform infrared spectroscopy (FT-IR). The phenanthrene degradation was studied from both in the strain pure cultures and from the defined consortium. Our results showed that the application of XAD2 preloaded with phenanthrene culture systems represents a selective environment towards substrate colonizing bacterial species and allow us study the different strategies to enhance the PAH uptake and allow the bacterial survival in oligotrophic conditions.
Materia
Ciencias Biológicas
Bacterial consortium
Adherence
PAH
Bioavailability
FT-IR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/8188

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network_name_str CIC Digital (CICBA)
spelling Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soilsLópez, Ana MaríaMedina, RocíoBosch, María Alejandra NievesYantorno, OsvaldoDel Panno, María T.Ciencias BiológicasBacterial consortiumAdherencePAHBioavailabilityFT-IRDue to the reduced bioavailability that characterizes chronically hydrocarbons contaminated soils, the bioaugmentation with cultures obtained by enrichment in solid phase systems, could be a promissing strategy to apply in aged contaminated soils. In this context, the objective of this work was to study four bacterial component obtained from a degrader phenanthrene consortium by using solid phases with a resin XAD2 preloaded with phenanthrene, and to rebuild a defined degrading consortium from them. The isolated strains were identified by 16S rRNA and phenotypically characterized using the conventional assays. Furthermore, their main phenotypic features and biochemical characteristics where analyzed by Fourier-transform infrared spectroscopy (FT-IR). The phenanthrene degradation was studied from both in the strain pure cultures and from the defined consortium. Our results showed that the application of XAD2 preloaded with phenanthrene culture systems represents a selective environment towards substrate colonizing bacterial species and allow us study the different strategies to enhance the PAH uptake and allow the bacterial survival in oligotrophic conditions.2013-04info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/8188enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:02Zoai:digital.cic.gba.gob.ar:11746/8188Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:02.308CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soils
title Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soils
spellingShingle Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soils
López, Ana María
Ciencias Biológicas
Bacterial consortium
Adherence
PAH
Bioavailability
FT-IR
title_short Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soils
title_full Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soils
title_fullStr Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soils
title_full_unstemmed Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soils
title_sort Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soils
dc.creator.none.fl_str_mv López, Ana María
Medina, Rocío
Bosch, María Alejandra Nieves
Yantorno, Osvaldo
Del Panno, María T.
author López, Ana María
author_facet López, Ana María
Medina, Rocío
Bosch, María Alejandra Nieves
Yantorno, Osvaldo
Del Panno, María T.
author_role author
author2 Medina, Rocío
Bosch, María Alejandra Nieves
Yantorno, Osvaldo
Del Panno, María T.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Biológicas
Bacterial consortium
Adherence
PAH
Bioavailability
FT-IR
topic Ciencias Biológicas
Bacterial consortium
Adherence
PAH
Bioavailability
FT-IR
dc.description.none.fl_txt_mv Due to the reduced bioavailability that characterizes chronically hydrocarbons contaminated soils, the bioaugmentation with cultures obtained by enrichment in solid phase systems, could be a promissing strategy to apply in aged contaminated soils. In this context, the objective of this work was to study four bacterial component obtained from a degrader phenanthrene consortium by using solid phases with a resin XAD2 preloaded with phenanthrene, and to rebuild a defined degrading consortium from them. The isolated strains were identified by 16S rRNA and phenotypically characterized using the conventional assays. Furthermore, their main phenotypic features and biochemical characteristics where analyzed by Fourier-transform infrared spectroscopy (FT-IR). The phenanthrene degradation was studied from both in the strain pure cultures and from the defined consortium. Our results showed that the application of XAD2 preloaded with phenanthrene culture systems represents a selective environment towards substrate colonizing bacterial species and allow us study the different strategies to enhance the PAH uptake and allow the bacterial survival in oligotrophic conditions.
description Due to the reduced bioavailability that characterizes chronically hydrocarbons contaminated soils, the bioaugmentation with cultures obtained by enrichment in solid phase systems, could be a promissing strategy to apply in aged contaminated soils. In this context, the objective of this work was to study four bacterial component obtained from a degrader phenanthrene consortium by using solid phases with a resin XAD2 preloaded with phenanthrene, and to rebuild a defined degrading consortium from them. The isolated strains were identified by 16S rRNA and phenotypically characterized using the conventional assays. Furthermore, their main phenotypic features and biochemical characteristics where analyzed by Fourier-transform infrared spectroscopy (FT-IR). The phenanthrene degradation was studied from both in the strain pure cultures and from the defined consortium. Our results showed that the application of XAD2 preloaded with phenanthrene culture systems represents a selective environment towards substrate colonizing bacterial species and allow us study the different strategies to enhance the PAH uptake and allow the bacterial survival in oligotrophic conditions.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
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info:eu-repo/semantics/publishedVersion
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format conferenceObject
status_str publishedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/8188
url https://digital.cic.gba.gob.ar/handle/11746/8188
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
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instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
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collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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