Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soils
- Autores
- López, Ana María; Medina, Rocío; Bosch, María Alejandra Nieves; Yantorno, Osvaldo; Del Panno, María T.
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Due to the reduced bioavailability that characterizes chronically hydrocarbons contaminated soils, the bioaugmentation with cultures obtained by enrichment in solid phase systems, could be a promissing strategy to apply in aged contaminated soils. In this context, the objective of this work was to study four bacterial component obtained from a degrader phenanthrene consortium by using solid phases with a resin XAD2 preloaded with phenanthrene, and to rebuild a defined degrading consortium from them. The isolated strains were identified by 16S rRNA and phenotypically characterized using the conventional assays. Furthermore, their main phenotypic features and biochemical characteristics where analyzed by Fourier-transform infrared spectroscopy (FT-IR). The phenanthrene degradation was studied from both in the strain pure cultures and from the defined consortium. Our results showed that the application of XAD2 preloaded with phenanthrene culture systems represents a selective environment towards substrate colonizing bacterial species and allow us study the different strategies to enhance the PAH uptake and allow the bacterial survival in oligotrophic conditions.
- Materia
-
Ciencias Biológicas
Bacterial consortium
Adherence
PAH
Bioavailability
FT-IR - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc/4.0/
- Repositorio
- Institución
- Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
- OAI Identificador
- oai:digital.cic.gba.gob.ar:11746/8188
Ver los metadatos del registro completo
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Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soilsLópez, Ana MaríaMedina, RocíoBosch, María Alejandra NievesYantorno, OsvaldoDel Panno, María T.Ciencias BiológicasBacterial consortiumAdherencePAHBioavailabilityFT-IRDue to the reduced bioavailability that characterizes chronically hydrocarbons contaminated soils, the bioaugmentation with cultures obtained by enrichment in solid phase systems, could be a promissing strategy to apply in aged contaminated soils. In this context, the objective of this work was to study four bacterial component obtained from a degrader phenanthrene consortium by using solid phases with a resin XAD2 preloaded with phenanthrene, and to rebuild a defined degrading consortium from them. The isolated strains were identified by 16S rRNA and phenotypically characterized using the conventional assays. Furthermore, their main phenotypic features and biochemical characteristics where analyzed by Fourier-transform infrared spectroscopy (FT-IR). The phenanthrene degradation was studied from both in the strain pure cultures and from the defined consortium. Our results showed that the application of XAD2 preloaded with phenanthrene culture systems represents a selective environment towards substrate colonizing bacterial species and allow us study the different strategies to enhance the PAH uptake and allow the bacterial survival in oligotrophic conditions.2013-04info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/8188enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:02Zoai:digital.cic.gba.gob.ar:11746/8188Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:02.308CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse |
dc.title.none.fl_str_mv |
Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soils |
title |
Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soils |
spellingShingle |
Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soils López, Ana María Ciencias Biológicas Bacterial consortium Adherence PAH Bioavailability FT-IR |
title_short |
Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soils |
title_full |
Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soils |
title_fullStr |
Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soils |
title_full_unstemmed |
Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soils |
title_sort |
Study of a bacterial consortium adapted to low bioavailability to phenanthrene, as potential inoculant to chronically PAH contaminated soils |
dc.creator.none.fl_str_mv |
López, Ana María Medina, Rocío Bosch, María Alejandra Nieves Yantorno, Osvaldo Del Panno, María T. |
author |
López, Ana María |
author_facet |
López, Ana María Medina, Rocío Bosch, María Alejandra Nieves Yantorno, Osvaldo Del Panno, María T. |
author_role |
author |
author2 |
Medina, Rocío Bosch, María Alejandra Nieves Yantorno, Osvaldo Del Panno, María T. |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Ciencias Biológicas Bacterial consortium Adherence PAH Bioavailability FT-IR |
topic |
Ciencias Biológicas Bacterial consortium Adherence PAH Bioavailability FT-IR |
dc.description.none.fl_txt_mv |
Due to the reduced bioavailability that characterizes chronically hydrocarbons contaminated soils, the bioaugmentation with cultures obtained by enrichment in solid phase systems, could be a promissing strategy to apply in aged contaminated soils. In this context, the objective of this work was to study four bacterial component obtained from a degrader phenanthrene consortium by using solid phases with a resin XAD2 preloaded with phenanthrene, and to rebuild a defined degrading consortium from them. The isolated strains were identified by 16S rRNA and phenotypically characterized using the conventional assays. Furthermore, their main phenotypic features and biochemical characteristics where analyzed by Fourier-transform infrared spectroscopy (FT-IR). The phenanthrene degradation was studied from both in the strain pure cultures and from the defined consortium. Our results showed that the application of XAD2 preloaded with phenanthrene culture systems represents a selective environment towards substrate colonizing bacterial species and allow us study the different strategies to enhance the PAH uptake and allow the bacterial survival in oligotrophic conditions. |
description |
Due to the reduced bioavailability that characterizes chronically hydrocarbons contaminated soils, the bioaugmentation with cultures obtained by enrichment in solid phase systems, could be a promissing strategy to apply in aged contaminated soils. In this context, the objective of this work was to study four bacterial component obtained from a degrader phenanthrene consortium by using solid phases with a resin XAD2 preloaded with phenanthrene, and to rebuild a defined degrading consortium from them. The isolated strains were identified by 16S rRNA and phenotypically characterized using the conventional assays. Furthermore, their main phenotypic features and biochemical characteristics where analyzed by Fourier-transform infrared spectroscopy (FT-IR). The phenanthrene degradation was studied from both in the strain pure cultures and from the defined consortium. Our results showed that the application of XAD2 preloaded with phenanthrene culture systems represents a selective environment towards substrate colonizing bacterial species and allow us study the different strategies to enhance the PAH uptake and allow the bacterial survival in oligotrophic conditions. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://digital.cic.gba.gob.ar/handle/11746/8188 |
url |
https://digital.cic.gba.gob.ar/handle/11746/8188 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
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application/pdf application/pdf |
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reponame:CIC Digital (CICBA) instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires instacron:CICBA |
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CIC Digital (CICBA) |
collection |
CIC Digital (CICBA) |
instname_str |
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
instacron_str |
CICBA |
institution |
CICBA |
repository.name.fl_str_mv |
CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
repository.mail.fl_str_mv |
marisa.degiusti@sedici.unlp.edu.ar |
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13.070432 |