Increased cellular uptake of lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles due to surface modification with folic acid

Autores
Feuser, Paulo Emilio; Carpio Arévalo, Juan Marcelo; Lima, Enio Junior; Rodrigues Rossi, Gustavo; Trindade, Edvaldo da Silva; Merlin Rocha, Maria Eliane; Virtuoso Jacques, Amanda; Ricci Júnior, Eduardo; Santos Silva, Maria Claudia; Sayer, Claudia; Hermes de Araújo, Pedro H.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lauryl gallate loaded in superparamagnetic poly (methyl methacrylate) nanoparticles surface modified with folic acid were synthesized by miniemulsion polymerization in just one step. In vitro biocompatibility and cytotoxicity assays on L929 (murine fibroblast), human red blood, and HeLa (uterine colon cancer) cells were performed. The effect of folic acid at the nanoparticles surface was evaluated through cellular uptake assays in HeLa cells. Resultsshowed that the presence of folic acid did not affect substantially the polymer particle size (~120 nm), the superparamagnetic behavior, the encapsulation efficiency of lauryl gallate (~87 %), the Zeta potential (~38 mV) of thepolymeric nanoparticles or the release profile of lauryl gallate. The release profile of lauryl gallate from superparamagnetic poly(methyl methacrylate) nanoparticles presented an initial burst effect (0?1 h) followed by a slow andsustained release, indicating a biphasic release system.Lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles with folic acid did not present cytotoxicity effects on L929 and human red blood cells. However, free lauryl gallate presented significant cytotoxic effects on L929 and human red blood cells at all tested concentrations. The presence of folic acid increased the cytotoxicity of lauryl gallate loaded in nanoparticles on HeLa cells due to a higher cellular uptake when HeLa cells were incubated at 37 °C. On the other hand, when the nanoparticles were incubated at low temperature (4 °C) cellular uptake was not observed, suggesting that the uptake occurred by folate receptor mediated energy-dependent endocytosis. Based on presented results our work suggeststhat this carrier system can be an excellent alternative in targeted drug delivery by folate receptor.
Fil: Feuser, Paulo Emilio. Universidade Federal de Santa Catarina; Brasil
Fil: Carpio Arévalo, Juan Marcelo. Universidade Federal do Paraná; Brasil
Fil: Lima, Enio Junior. Comisión Nacional de Energía Atómica. Gerencia del Área de Investigación y Aplicaciones No Nucleares. Gerencia de Física. Laboratorio de Resonancias Magnéticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
Fil: Rodrigues Rossi, Gustavo. Universidade Federal do Paraná; Brasil
Fil: Trindade, Edvaldo da Silva. Universidade Federal do Paraná; Brasil
Fil: Merlin Rocha, Maria Eliane. Universidade Federal do Paraná; Brasil
Fil: Virtuoso Jacques, Amanda. Universidade Federal de Santa Catarina; Brasil
Fil: Ricci Júnior, Eduardo. Universidade Federal do Rio de Janeiro; Brasil
Fil: Santos Silva, Maria Claudia. Universidade Federal de Santa Catarina; Brasil
Fil: Sayer, Claudia. Universidade Federal de Santa Catarina; Brasil
Fil: Hermes de Araújo, Pedro H.. Universidade Federal de Santa Catarina; Brasil
Materia
Polimeric Nanoparticles
Superparamagnetism
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/74376
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/74376
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Increased cellular uptake of lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles due to surface modification with folic acidFeuser, Paulo EmilioCarpio Arévalo, Juan MarceloLima, Enio JuniorRodrigues Rossi, GustavoTrindade, Edvaldo da SilvaMerlin Rocha, Maria ElianeVirtuoso Jacques, AmandaRicci Júnior, EduardoSantos Silva, Maria ClaudiaSayer, ClaudiaHermes de Araújo, Pedro H.Polimeric NanoparticlesSuperparamagnetismhttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2Lauryl gallate loaded in superparamagnetic poly (methyl methacrylate) nanoparticles surface modified with folic acid were synthesized by miniemulsion polymerization in just one step. In vitro biocompatibility and cytotoxicity assays on L929 (murine fibroblast), human red blood, and HeLa (uterine colon cancer) cells were performed. The effect of folic acid at the nanoparticles surface was evaluated through cellular uptake assays in HeLa cells. Resultsshowed that the presence of folic acid did not affect substantially the polymer particle size (~120 nm), the superparamagnetic behavior, the encapsulation efficiency of lauryl gallate (~87 %), the Zeta potential (~38 mV) of thepolymeric nanoparticles or the release profile of lauryl gallate. The release profile of lauryl gallate from superparamagnetic poly(methyl methacrylate) nanoparticles presented an initial burst effect (0?1 h) followed by a slow andsustained release, indicating a biphasic release system.Lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles with folic acid did not present cytotoxicity effects on L929 and human red blood cells. However, free lauryl gallate presented significant cytotoxic effects on L929 and human red blood cells at all tested concentrations. The presence of folic acid increased the cytotoxicity of lauryl gallate loaded in nanoparticles on HeLa cells due to a higher cellular uptake when HeLa cells were incubated at 37 °C. On the other hand, when the nanoparticles were incubated at low temperature (4 °C) cellular uptake was not observed, suggesting that the uptake occurred by folate receptor mediated energy-dependent endocytosis. Based on presented results our work suggeststhat this carrier system can be an excellent alternative in targeted drug delivery by folate receptor.Fil: Feuser, Paulo Emilio. Universidade Federal de Santa Catarina; BrasilFil: Carpio Arévalo, Juan Marcelo. Universidade Federal do Paraná; BrasilFil: Lima, Enio Junior. Comisión Nacional de Energía Atómica. Gerencia del Área de Investigación y Aplicaciones No Nucleares. Gerencia de Física. Laboratorio de Resonancias Magnéticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; ArgentinaFil: Rodrigues Rossi, Gustavo. Universidade Federal do Paraná; BrasilFil: Trindade, Edvaldo da Silva. Universidade Federal do Paraná; BrasilFil: Merlin Rocha, Maria Eliane. Universidade Federal do Paraná; BrasilFil: Virtuoso Jacques, Amanda. Universidade Federal de Santa Catarina; BrasilFil: Ricci Júnior, Eduardo. Universidade Federal do Rio de Janeiro; BrasilFil: Santos Silva, Maria Claudia. Universidade Federal de Santa Catarina; BrasilFil: Sayer, Claudia. Universidade Federal de Santa Catarina; BrasilFil: Hermes de Araújo, Pedro H.. Universidade Federal de Santa Catarina; BrasilSpringer2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/74376Feuser, Paulo Emilio; Carpio Arévalo, Juan Marcelo; Lima, Enio Junior; Rodrigues Rossi, Gustavo; Trindade, Edvaldo da Silva; et al.; Increased cellular uptake of lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles due to surface modification with folic acid; Springer; Journal of Materials Science: Materials In Medicine; 27; 12-2016; 185-2000957-4530CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10856-016-5796-0info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10856-016-5796-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:12Zoai:ri.conicet.gov.ar:11336/74376instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:12.996CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Increased cellular uptake of lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles due to surface modification with folic acid
title Increased cellular uptake of lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles due to surface modification with folic acid
spellingShingle Increased cellular uptake of lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles due to surface modification with folic acid
Feuser, Paulo Emilio
Polimeric Nanoparticles
Superparamagnetism
title_short Increased cellular uptake of lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles due to surface modification with folic acid
title_full Increased cellular uptake of lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles due to surface modification with folic acid
title_fullStr Increased cellular uptake of lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles due to surface modification with folic acid
title_full_unstemmed Increased cellular uptake of lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles due to surface modification with folic acid
title_sort Increased cellular uptake of lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles due to surface modification with folic acid
dc.creator.none.fl_str_mv Feuser, Paulo Emilio
Carpio Arévalo, Juan Marcelo
Lima, Enio Junior
Rodrigues Rossi, Gustavo
Trindade, Edvaldo da Silva
Merlin Rocha, Maria Eliane
Virtuoso Jacques, Amanda
Ricci Júnior, Eduardo
Santos Silva, Maria Claudia
Sayer, Claudia
Hermes de Araújo, Pedro H.
author Feuser, Paulo Emilio
author_facet Feuser, Paulo Emilio
Carpio Arévalo, Juan Marcelo
Lima, Enio Junior
Rodrigues Rossi, Gustavo
Trindade, Edvaldo da Silva
Merlin Rocha, Maria Eliane
Virtuoso Jacques, Amanda
Ricci Júnior, Eduardo
Santos Silva, Maria Claudia
Sayer, Claudia
Hermes de Araújo, Pedro H.
author_role author
author2 Carpio Arévalo, Juan Marcelo
Lima, Enio Junior
Rodrigues Rossi, Gustavo
Trindade, Edvaldo da Silva
Merlin Rocha, Maria Eliane
Virtuoso Jacques, Amanda
Ricci Júnior, Eduardo
Santos Silva, Maria Claudia
Sayer, Claudia
Hermes de Araújo, Pedro H.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Polimeric Nanoparticles
Superparamagnetism
topic Polimeric Nanoparticles
Superparamagnetism
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Lauryl gallate loaded in superparamagnetic poly (methyl methacrylate) nanoparticles surface modified with folic acid were synthesized by miniemulsion polymerization in just one step. In vitro biocompatibility and cytotoxicity assays on L929 (murine fibroblast), human red blood, and HeLa (uterine colon cancer) cells were performed. The effect of folic acid at the nanoparticles surface was evaluated through cellular uptake assays in HeLa cells. Resultsshowed that the presence of folic acid did not affect substantially the polymer particle size (~120 nm), the superparamagnetic behavior, the encapsulation efficiency of lauryl gallate (~87 %), the Zeta potential (~38 mV) of thepolymeric nanoparticles or the release profile of lauryl gallate. The release profile of lauryl gallate from superparamagnetic poly(methyl methacrylate) nanoparticles presented an initial burst effect (0?1 h) followed by a slow andsustained release, indicating a biphasic release system.Lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles with folic acid did not present cytotoxicity effects on L929 and human red blood cells. However, free lauryl gallate presented significant cytotoxic effects on L929 and human red blood cells at all tested concentrations. The presence of folic acid increased the cytotoxicity of lauryl gallate loaded in nanoparticles on HeLa cells due to a higher cellular uptake when HeLa cells were incubated at 37 °C. On the other hand, when the nanoparticles were incubated at low temperature (4 °C) cellular uptake was not observed, suggesting that the uptake occurred by folate receptor mediated energy-dependent endocytosis. Based on presented results our work suggeststhat this carrier system can be an excellent alternative in targeted drug delivery by folate receptor.
Fil: Feuser, Paulo Emilio. Universidade Federal de Santa Catarina; Brasil
Fil: Carpio Arévalo, Juan Marcelo. Universidade Federal do Paraná; Brasil
Fil: Lima, Enio Junior. Comisión Nacional de Energía Atómica. Gerencia del Área de Investigación y Aplicaciones No Nucleares. Gerencia de Física. Laboratorio de Resonancias Magnéticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
Fil: Rodrigues Rossi, Gustavo. Universidade Federal do Paraná; Brasil
Fil: Trindade, Edvaldo da Silva. Universidade Federal do Paraná; Brasil
Fil: Merlin Rocha, Maria Eliane. Universidade Federal do Paraná; Brasil
Fil: Virtuoso Jacques, Amanda. Universidade Federal de Santa Catarina; Brasil
Fil: Ricci Júnior, Eduardo. Universidade Federal do Rio de Janeiro; Brasil
Fil: Santos Silva, Maria Claudia. Universidade Federal de Santa Catarina; Brasil
Fil: Sayer, Claudia. Universidade Federal de Santa Catarina; Brasil
Fil: Hermes de Araújo, Pedro H.. Universidade Federal de Santa Catarina; Brasil
description Lauryl gallate loaded in superparamagnetic poly (methyl methacrylate) nanoparticles surface modified with folic acid were synthesized by miniemulsion polymerization in just one step. In vitro biocompatibility and cytotoxicity assays on L929 (murine fibroblast), human red blood, and HeLa (uterine colon cancer) cells were performed. The effect of folic acid at the nanoparticles surface was evaluated through cellular uptake assays in HeLa cells. Resultsshowed that the presence of folic acid did not affect substantially the polymer particle size (~120 nm), the superparamagnetic behavior, the encapsulation efficiency of lauryl gallate (~87 %), the Zeta potential (~38 mV) of thepolymeric nanoparticles or the release profile of lauryl gallate. The release profile of lauryl gallate from superparamagnetic poly(methyl methacrylate) nanoparticles presented an initial burst effect (0?1 h) followed by a slow andsustained release, indicating a biphasic release system.Lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles with folic acid did not present cytotoxicity effects on L929 and human red blood cells. However, free lauryl gallate presented significant cytotoxic effects on L929 and human red blood cells at all tested concentrations. The presence of folic acid increased the cytotoxicity of lauryl gallate loaded in nanoparticles on HeLa cells due to a higher cellular uptake when HeLa cells were incubated at 37 °C. On the other hand, when the nanoparticles were incubated at low temperature (4 °C) cellular uptake was not observed, suggesting that the uptake occurred by folate receptor mediated energy-dependent endocytosis. Based on presented results our work suggeststhat this carrier system can be an excellent alternative in targeted drug delivery by folate receptor.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/74376
Feuser, Paulo Emilio; Carpio Arévalo, Juan Marcelo; Lima, Enio Junior; Rodrigues Rossi, Gustavo; Trindade, Edvaldo da Silva; et al.; Increased cellular uptake of lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles due to surface modification with folic acid; Springer; Journal of Materials Science: Materials In Medicine; 27; 12-2016; 185-200
0957-4530
CONICET Digital
CONICET
url http://hdl.handle.net/11336/74376
identifier_str_mv Feuser, Paulo Emilio; Carpio Arévalo, Juan Marcelo; Lima, Enio Junior; Rodrigues Rossi, Gustavo; Trindade, Edvaldo da Silva; et al.; Increased cellular uptake of lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles due to surface modification with folic acid; Springer; Journal of Materials Science: Materials In Medicine; 27; 12-2016; 185-200
0957-4530
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s10856-016-5796-0
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10856-016-5796-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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