Mucor circinelloides whole-cells as a biocatalyst for the production 3 of ethyl esters based on babassu oil

Autores
Andrade, Grazielle S. S.; Carvalho, Ana K. F.; Romero, Cintia Mariana; Oliveira, Pedro C.; Castro, Heizir F. de
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The intracellular lipase production by Mucor circinelloides URM 4182 was investigated through a step-by-step strategy to attain immobilized whole-cells with high lipase activity. Physicochemical parameters, such as carbon and nitrogen sources, inoculum size and aeration, were studied to determine the optimum conditions for both lipase production and immobilization in polyurethane support. Olive oil and soybean peptone were found to be the best carbon and nitrogen sources, respectively, to enhance the intracellular lipase activity. Low inoculum level and poor aeration rate also provided suitable conditions to attain high lipase activity (64.8 +/- A 0.8 U g(-1)). The transesterification activity of the immobilized whole- cells was assayed and optimal reaction conditions for the ethanolysis of babassu oil were determined by experimental design. Statistical analysis showed that M. circinelloides whole-cells were able to produce ethyl esters at all tested conditions, with the highest yield attained (98.1 %) at 35 A degrees C using an 1:6 oil-to-ethanol molar ratio. The biocatalyst operational stability was also assayed in a continuous packed bed reactor (PBR) charged with glutaraldehyde (GA) and Aliquat-treated cells revealing half-life of 43.0 +/- A 0.5 and 20.0 +/- A 0.8 days, respectively. These results indicate the potential of immobilized M. circinelloides URM 4182 whole-cells as a low-cost alternative to conventional biocatalysts in the production of ethyl esters from babassu oil. (AU)
Fil: Andrade, Grazielle S. S.. Universidade de Sao Paulo. Instituto de Biociências; Brasil
Fil: Carvalho, Ana K. F.. Universidade de Sao Paulo. Instituto de Biociências; Brasil
Fil: Romero, Cintia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina
Fil: Oliveira, Pedro C.. Universidade de Sao Paulo. Instituto de Biociências; Brasil
Fil: Castro, Heizir F. de. Universidade de Sao Paulo. Instituto de Biociências; Brasil
Materia
Lipase
Mucor Circinelloide
Immobilized Whole-Cells
Biotalaysis
Ethyl Esters
Polyurethane
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/17186
Acceder
id CONICETDig_5666ae2daab9f57e6c6497aa0cf57918
oai_identifier_str oai:ri.conicet.gov.ar:11336/17186
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mucor circinelloides whole-cells as a biocatalyst for the production 3 of ethyl esters based on babassu oilAndrade, Grazielle S. S.Carvalho, Ana K. F.Romero, Cintia MarianaOliveira, Pedro C.Castro, Heizir F. deLipaseMucor CircinelloideImmobilized Whole-CellsBiotalaysisEthyl EstersPolyurethanehttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The intracellular lipase production by Mucor circinelloides URM 4182 was investigated through a step-by-step strategy to attain immobilized whole-cells with high lipase activity. Physicochemical parameters, such as carbon and nitrogen sources, inoculum size and aeration, were studied to determine the optimum conditions for both lipase production and immobilization in polyurethane support. Olive oil and soybean peptone were found to be the best carbon and nitrogen sources, respectively, to enhance the intracellular lipase activity. Low inoculum level and poor aeration rate also provided suitable conditions to attain high lipase activity (64.8 +/- A 0.8 U g(-1)). The transesterification activity of the immobilized whole- cells was assayed and optimal reaction conditions for the ethanolysis of babassu oil were determined by experimental design. Statistical analysis showed that M. circinelloides whole-cells were able to produce ethyl esters at all tested conditions, with the highest yield attained (98.1 %) at 35 A degrees C using an 1:6 oil-to-ethanol molar ratio. The biocatalyst operational stability was also assayed in a continuous packed bed reactor (PBR) charged with glutaraldehyde (GA) and Aliquat-treated cells revealing half-life of 43.0 +/- A 0.5 and 20.0 +/- A 0.8 days, respectively. These results indicate the potential of immobilized M. circinelloides URM 4182 whole-cells as a low-cost alternative to conventional biocatalysts in the production of ethyl esters from babassu oil. (AU)Fil: Andrade, Grazielle S. S.. Universidade de Sao Paulo. Instituto de Biociências; BrasilFil: Carvalho, Ana K. F.. Universidade de Sao Paulo. Instituto de Biociências; BrasilFil: Romero, Cintia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); ArgentinaFil: Oliveira, Pedro C.. Universidade de Sao Paulo. Instituto de Biociências; BrasilFil: Castro, Heizir F. de. Universidade de Sao Paulo. Instituto de Biociências; BrasilSpringer2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17186Andrade, Grazielle S. S.; Carvalho, Ana K. F.; Romero, Cintia Mariana; Oliveira, Pedro C.; Castro, Heizir F. de; Mucor circinelloides whole-cells as a biocatalyst for the production 3 of ethyl esters based on babassu oil; Springer; Bioprocess And Biosystems Engineering; 37; 12; 6-2014; 2539-25481615-7591enginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00449-014-1231-4info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00449-014-1231-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:59Zoai:ri.conicet.gov.ar:11336/17186instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:59.917CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mucor circinelloides whole-cells as a biocatalyst for the production 3 of ethyl esters based on babassu oil
title Mucor circinelloides whole-cells as a biocatalyst for the production 3 of ethyl esters based on babassu oil
spellingShingle Mucor circinelloides whole-cells as a biocatalyst for the production 3 of ethyl esters based on babassu oil
Andrade, Grazielle S. S.
Lipase
Mucor Circinelloide
Immobilized Whole-Cells
Biotalaysis
Ethyl Esters
Polyurethane
title_short Mucor circinelloides whole-cells as a biocatalyst for the production 3 of ethyl esters based on babassu oil
title_full Mucor circinelloides whole-cells as a biocatalyst for the production 3 of ethyl esters based on babassu oil
title_fullStr Mucor circinelloides whole-cells as a biocatalyst for the production 3 of ethyl esters based on babassu oil
title_full_unstemmed Mucor circinelloides whole-cells as a biocatalyst for the production 3 of ethyl esters based on babassu oil
title_sort Mucor circinelloides whole-cells as a biocatalyst for the production 3 of ethyl esters based on babassu oil
dc.creator.none.fl_str_mv Andrade, Grazielle S. S.
Carvalho, Ana K. F.
Romero, Cintia Mariana
Oliveira, Pedro C.
Castro, Heizir F. de
author Andrade, Grazielle S. S.
author_facet Andrade, Grazielle S. S.
Carvalho, Ana K. F.
Romero, Cintia Mariana
Oliveira, Pedro C.
Castro, Heizir F. de
author_role author
author2 Carvalho, Ana K. F.
Romero, Cintia Mariana
Oliveira, Pedro C.
Castro, Heizir F. de
author2_role author
author
author
author
dc.subject.none.fl_str_mv Lipase
Mucor Circinelloide
Immobilized Whole-Cells
Biotalaysis
Ethyl Esters
Polyurethane
topic Lipase
Mucor Circinelloide
Immobilized Whole-Cells
Biotalaysis
Ethyl Esters
Polyurethane
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The intracellular lipase production by Mucor circinelloides URM 4182 was investigated through a step-by-step strategy to attain immobilized whole-cells with high lipase activity. Physicochemical parameters, such as carbon and nitrogen sources, inoculum size and aeration, were studied to determine the optimum conditions for both lipase production and immobilization in polyurethane support. Olive oil and soybean peptone were found to be the best carbon and nitrogen sources, respectively, to enhance the intracellular lipase activity. Low inoculum level and poor aeration rate also provided suitable conditions to attain high lipase activity (64.8 +/- A 0.8 U g(-1)). The transesterification activity of the immobilized whole- cells was assayed and optimal reaction conditions for the ethanolysis of babassu oil were determined by experimental design. Statistical analysis showed that M. circinelloides whole-cells were able to produce ethyl esters at all tested conditions, with the highest yield attained (98.1 %) at 35 A degrees C using an 1:6 oil-to-ethanol molar ratio. The biocatalyst operational stability was also assayed in a continuous packed bed reactor (PBR) charged with glutaraldehyde (GA) and Aliquat-treated cells revealing half-life of 43.0 +/- A 0.5 and 20.0 +/- A 0.8 days, respectively. These results indicate the potential of immobilized M. circinelloides URM 4182 whole-cells as a low-cost alternative to conventional biocatalysts in the production of ethyl esters from babassu oil. (AU)
Fil: Andrade, Grazielle S. S.. Universidade de Sao Paulo. Instituto de Biociências; Brasil
Fil: Carvalho, Ana K. F.. Universidade de Sao Paulo. Instituto de Biociências; Brasil
Fil: Romero, Cintia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina
Fil: Oliveira, Pedro C.. Universidade de Sao Paulo. Instituto de Biociências; Brasil
Fil: Castro, Heizir F. de. Universidade de Sao Paulo. Instituto de Biociências; Brasil
description The intracellular lipase production by Mucor circinelloides URM 4182 was investigated through a step-by-step strategy to attain immobilized whole-cells with high lipase activity. Physicochemical parameters, such as carbon and nitrogen sources, inoculum size and aeration, were studied to determine the optimum conditions for both lipase production and immobilization in polyurethane support. Olive oil and soybean peptone were found to be the best carbon and nitrogen sources, respectively, to enhance the intracellular lipase activity. Low inoculum level and poor aeration rate also provided suitable conditions to attain high lipase activity (64.8 +/- A 0.8 U g(-1)). The transesterification activity of the immobilized whole- cells was assayed and optimal reaction conditions for the ethanolysis of babassu oil were determined by experimental design. Statistical analysis showed that M. circinelloides whole-cells were able to produce ethyl esters at all tested conditions, with the highest yield attained (98.1 %) at 35 A degrees C using an 1:6 oil-to-ethanol molar ratio. The biocatalyst operational stability was also assayed in a continuous packed bed reactor (PBR) charged with glutaraldehyde (GA) and Aliquat-treated cells revealing half-life of 43.0 +/- A 0.5 and 20.0 +/- A 0.8 days, respectively. These results indicate the potential of immobilized M. circinelloides URM 4182 whole-cells as a low-cost alternative to conventional biocatalysts in the production of ethyl esters from babassu oil. (AU)
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/17186
Andrade, Grazielle S. S.; Carvalho, Ana K. F.; Romero, Cintia Mariana; Oliveira, Pedro C.; Castro, Heizir F. de; Mucor circinelloides whole-cells as a biocatalyst for the production 3 of ethyl esters based on babassu oil; Springer; Bioprocess And Biosystems Engineering; 37; 12; 6-2014; 2539-2548
1615-7591
url http://hdl.handle.net/11336/17186
identifier_str_mv Andrade, Grazielle S. S.; Carvalho, Ana K. F.; Romero, Cintia Mariana; Oliveira, Pedro C.; Castro, Heizir F. de; Mucor circinelloides whole-cells as a biocatalyst for the production 3 of ethyl esters based on babassu oil; Springer; Bioprocess And Biosystems Engineering; 37; 12; 6-2014; 2539-2548
1615-7591
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00449-014-1231-4
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00449-014-1231-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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