A subunit of protein kinase a regulates growth and differentiation in the fungus mucor circinelloides

Autores
Ocampo, Josefina; Fernández Núñez, Lucas Matías; Silva, F.; Pereyra, Elba Nora; Moreno, Silvia Margarita; Garre, V.; Rossi, Silvia Graciela
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The cyclic AMP (cAMP)-dependent protein kinase A (PKA) signaling pathway plays a role in regulating development, growth, and virulence in a number of fungi. To determine whether PKA plays a similar function in zygomycete fungi, a mutant of Mucor circinelloides was generated that lacks pkaR1, one of the regulatory subunits of PKA. The mutant showed a reduction in growth and alterations in germination rates, cell volume, germ tube length, and asexual sporulation. The lack of pkaR1 gene resulted in a highly decreased, but not null, cAMP binding activity and in a protein kinase activity that was still dependent on cAMP, although with a higher -/+ cAMP activity ratio, suggesting the existence of other cAMP binding activities. Consequently, three proteins analogous to pkaR1 were predicted from the recently sequenced genome of M. circinelloides and were named pkaR2, pkaR3, and pkaR4. Two of the proteins, pkaR2 and pkaR3, with cAMP binding activity were isolated from the wild-type strain and identified by mass spectrometry. The expression of all genes was detected at the mRNA level by semiquantitative reverse transcription-PCR, and they showed a differential expression at different developmental stages. This is the first time that a fungus is reported to have more than one gene encoding the regulatory subunit of PKA. © 2009, American Society for Microbiology.
Fil: Ocampo, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Fernández Núñez, Lucas Matías. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Silva, F.. Universidad de Murcia; España
Fil: Pereyra, Elba Nora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Moreno, Silvia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Garre, V.. Universidad de Murcia; España
Fil: Rossi, Silvia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Materia
Protein kinase A
Mucor circinelloides
regulatory subunit
isoforms
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67843
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/67843
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A subunit of protein kinase a regulates growth and differentiation in the fungus mucor circinelloidesOcampo, JosefinaFernández Núñez, Lucas MatíasSilva, F.Pereyra, Elba NoraMoreno, Silvia MargaritaGarre, V.Rossi, Silvia GracielaProtein kinase AMucor circinelloidesregulatory subunitisoformshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The cyclic AMP (cAMP)-dependent protein kinase A (PKA) signaling pathway plays a role in regulating development, growth, and virulence in a number of fungi. To determine whether PKA plays a similar function in zygomycete fungi, a mutant of Mucor circinelloides was generated that lacks pkaR1, one of the regulatory subunits of PKA. The mutant showed a reduction in growth and alterations in germination rates, cell volume, germ tube length, and asexual sporulation. The lack of pkaR1 gene resulted in a highly decreased, but not null, cAMP binding activity and in a protein kinase activity that was still dependent on cAMP, although with a higher -/+ cAMP activity ratio, suggesting the existence of other cAMP binding activities. Consequently, three proteins analogous to pkaR1 were predicted from the recently sequenced genome of M. circinelloides and were named pkaR2, pkaR3, and pkaR4. Two of the proteins, pkaR2 and pkaR3, with cAMP binding activity were isolated from the wild-type strain and identified by mass spectrometry. The expression of all genes was detected at the mRNA level by semiquantitative reverse transcription-PCR, and they showed a differential expression at different developmental stages. This is the first time that a fungus is reported to have more than one gene encoding the regulatory subunit of PKA. © 2009, American Society for Microbiology.Fil: Ocampo, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Fernández Núñez, Lucas Matías. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Silva, F.. Universidad de Murcia; EspañaFil: Pereyra, Elba Nora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Moreno, Silvia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Garre, V.. Universidad de Murcia; EspañaFil: Rossi, Silvia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaAmerican Society for Microbiology2009-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67843Ocampo, Josefina; Fernández Núñez, Lucas Matías; Silva, F.; Pereyra, Elba Nora; Moreno, Silvia Margarita; et al.; A subunit of protein kinase a regulates growth and differentiation in the fungus mucor circinelloides; American Society for Microbiology; Eukaryotic Cell; 8; 7; 7-2009; 933-9441535-9778CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ec.asm.org/content/8/7/933info:eu-repo/semantics/altIdentifier/doi/10.1128/EC.00026-09info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:34Zoai:ri.conicet.gov.ar:11336/67843instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:35.317CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A subunit of protein kinase a regulates growth and differentiation in the fungus mucor circinelloides
title A subunit of protein kinase a regulates growth and differentiation in the fungus mucor circinelloides
spellingShingle A subunit of protein kinase a regulates growth and differentiation in the fungus mucor circinelloides
Ocampo, Josefina
Protein kinase A
Mucor circinelloides
regulatory subunit
isoforms
title_short A subunit of protein kinase a regulates growth and differentiation in the fungus mucor circinelloides
title_full A subunit of protein kinase a regulates growth and differentiation in the fungus mucor circinelloides
title_fullStr A subunit of protein kinase a regulates growth and differentiation in the fungus mucor circinelloides
title_full_unstemmed A subunit of protein kinase a regulates growth and differentiation in the fungus mucor circinelloides
title_sort A subunit of protein kinase a regulates growth and differentiation in the fungus mucor circinelloides
dc.creator.none.fl_str_mv Ocampo, Josefina
Fernández Núñez, Lucas Matías
Silva, F.
Pereyra, Elba Nora
Moreno, Silvia Margarita
Garre, V.
Rossi, Silvia Graciela
author Ocampo, Josefina
author_facet Ocampo, Josefina
Fernández Núñez, Lucas Matías
Silva, F.
Pereyra, Elba Nora
Moreno, Silvia Margarita
Garre, V.
Rossi, Silvia Graciela
author_role author
author2 Fernández Núñez, Lucas Matías
Silva, F.
Pereyra, Elba Nora
Moreno, Silvia Margarita
Garre, V.
Rossi, Silvia Graciela
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Protein kinase A
Mucor circinelloides
regulatory subunit
isoforms
topic Protein kinase A
Mucor circinelloides
regulatory subunit
isoforms
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The cyclic AMP (cAMP)-dependent protein kinase A (PKA) signaling pathway plays a role in regulating development, growth, and virulence in a number of fungi. To determine whether PKA plays a similar function in zygomycete fungi, a mutant of Mucor circinelloides was generated that lacks pkaR1, one of the regulatory subunits of PKA. The mutant showed a reduction in growth and alterations in germination rates, cell volume, germ tube length, and asexual sporulation. The lack of pkaR1 gene resulted in a highly decreased, but not null, cAMP binding activity and in a protein kinase activity that was still dependent on cAMP, although with a higher -/+ cAMP activity ratio, suggesting the existence of other cAMP binding activities. Consequently, three proteins analogous to pkaR1 were predicted from the recently sequenced genome of M. circinelloides and were named pkaR2, pkaR3, and pkaR4. Two of the proteins, pkaR2 and pkaR3, with cAMP binding activity were isolated from the wild-type strain and identified by mass spectrometry. The expression of all genes was detected at the mRNA level by semiquantitative reverse transcription-PCR, and they showed a differential expression at different developmental stages. This is the first time that a fungus is reported to have more than one gene encoding the regulatory subunit of PKA. © 2009, American Society for Microbiology.
Fil: Ocampo, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Fernández Núñez, Lucas Matías. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Silva, F.. Universidad de Murcia; España
Fil: Pereyra, Elba Nora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Moreno, Silvia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Garre, V.. Universidad de Murcia; España
Fil: Rossi, Silvia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
description The cyclic AMP (cAMP)-dependent protein kinase A (PKA) signaling pathway plays a role in regulating development, growth, and virulence in a number of fungi. To determine whether PKA plays a similar function in zygomycete fungi, a mutant of Mucor circinelloides was generated that lacks pkaR1, one of the regulatory subunits of PKA. The mutant showed a reduction in growth and alterations in germination rates, cell volume, germ tube length, and asexual sporulation. The lack of pkaR1 gene resulted in a highly decreased, but not null, cAMP binding activity and in a protein kinase activity that was still dependent on cAMP, although with a higher -/+ cAMP activity ratio, suggesting the existence of other cAMP binding activities. Consequently, three proteins analogous to pkaR1 were predicted from the recently sequenced genome of M. circinelloides and were named pkaR2, pkaR3, and pkaR4. Two of the proteins, pkaR2 and pkaR3, with cAMP binding activity were isolated from the wild-type strain and identified by mass spectrometry. The expression of all genes was detected at the mRNA level by semiquantitative reverse transcription-PCR, and they showed a differential expression at different developmental stages. This is the first time that a fungus is reported to have more than one gene encoding the regulatory subunit of PKA. © 2009, American Society for Microbiology.
publishDate 2009
dc.date.none.fl_str_mv 2009-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67843
Ocampo, Josefina; Fernández Núñez, Lucas Matías; Silva, F.; Pereyra, Elba Nora; Moreno, Silvia Margarita; et al.; A subunit of protein kinase a regulates growth and differentiation in the fungus mucor circinelloides; American Society for Microbiology; Eukaryotic Cell; 8; 7; 7-2009; 933-944
1535-9778
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67843
identifier_str_mv Ocampo, Josefina; Fernández Núñez, Lucas Matías; Silva, F.; Pereyra, Elba Nora; Moreno, Silvia Margarita; et al.; A subunit of protein kinase a regulates growth and differentiation in the fungus mucor circinelloides; American Society for Microbiology; Eukaryotic Cell; 8; 7; 7-2009; 933-944
1535-9778
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ec.asm.org/content/8/7/933
info:eu-repo/semantics/altIdentifier/doi/10.1128/EC.00026-09
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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