Mimicking enzymatic effects of cytochrome P450 by an efficient biosensor for in vitro detection of DNA damage

Autores
Jalalvand, Ali R.; Gholivand, Mohammad Bagher; Goicoechea, Hector Casimiro; Skov, Thomas; Mansouri, Kamran
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A novel biosensor for detecting DNA damage induced by benzo(a)pyrene (BP) and its metabolite was presented 18 in this work. The nafion-solubilized single wall carbon nanotubes-ionic liquid (SWCNTs-NA-IL) composite film 19 was prepared and then horseradish peroxidase (HRP) and double-stranded DNA were alternately assembled on 20 the composite film by the layer-by-layer method. The biosensor was characterized by cyclic voltammetry (CV), 21 electrochemical impedance spectroscopy (EIS), differential pulse voltammetry (DPV), scanning electron 22 microscopy (SEM) and computational methods. UVvis spectrophotometry was also used to investigate DNA 23 damage induced by BP and its metabolites in solution. The DNA biosensor was treated separately in BP, 24 hydrogen peroxide (H2O2) and in their mixture, respectively. The EIS analysis showed a decrease in the charge 25 transfer resistance at the DNA/HRP/SWCNTs-NA-IL/GCE incubated in a mixture of HRP and H2O2, because 26 HRP in the presence of H2O2 could mimic enzymatic effects of cytochrome P450 (CYP450) to metabolize BP 27 which could cause significant DNA damage and the exposed DNA bases reduced the electrostatic repulsion of the 28 negatively charged redox probe and leads to Faradaic impedance changes. Finally, a novel biosensor for BP 29 determination was developed and this method provided an indirect, and quantitative estimate of DNA damage in 30 vitro.
Fil: Jalalvand, Ali R.. Razi University. Department of Analytical Chemistry; Irán. Kermanshah University of Medical Sciences; Irán. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Química Analitica; Argentina
Fil: Gholivand, Mohammad Bagher. Razi University. Department of Analytical Chemistry; Irán
Fil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Química Analitica; Argentina
Fil: Skov, Thomas. Universidad de Copenhagen; Dinamarca
Fil: Mansouri, Kamran. Kermanshah University of Medical Sciences; Irán
Materia
Biosensor
Dna Damage
Benzo(A)Pyrene
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/17364
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/17364
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mimicking enzymatic effects of cytochrome P450 by an efficient biosensor for in vitro detection of DNA damageJalalvand, Ali R.Gholivand, Mohammad BagherGoicoechea, Hector CasimiroSkov, ThomasMansouri, KamranBiosensorDna DamageBenzo(A)Pyrenehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A novel biosensor for detecting DNA damage induced by benzo(a)pyrene (BP) and its metabolite was presented 18 in this work. The nafion-solubilized single wall carbon nanotubes-ionic liquid (SWCNTs-NA-IL) composite film 19 was prepared and then horseradish peroxidase (HRP) and double-stranded DNA were alternately assembled on 20 the composite film by the layer-by-layer method. The biosensor was characterized by cyclic voltammetry (CV), 21 electrochemical impedance spectroscopy (EIS), differential pulse voltammetry (DPV), scanning electron 22 microscopy (SEM) and computational methods. UVvis spectrophotometry was also used to investigate DNA 23 damage induced by BP and its metabolites in solution. The DNA biosensor was treated separately in BP, 24 hydrogen peroxide (H2O2) and in their mixture, respectively. The EIS analysis showed a decrease in the charge 25 transfer resistance at the DNA/HRP/SWCNTs-NA-IL/GCE incubated in a mixture of HRP and H2O2, because 26 HRP in the presence of H2O2 could mimic enzymatic effects of cytochrome P450 (CYP450) to metabolize BP 27 which could cause significant DNA damage and the exposed DNA bases reduced the electrostatic repulsion of the 28 negatively charged redox probe and leads to Faradaic impedance changes. Finally, a novel biosensor for BP 29 determination was developed and this method provided an indirect, and quantitative estimate of DNA damage in 30 vitro.Fil: Jalalvand, Ali R.. Razi University. Department of Analytical Chemistry; Irán. Kermanshah University of Medical Sciences; Irán. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Química Analitica; ArgentinaFil: Gholivand, Mohammad Bagher. Razi University. Department of Analytical Chemistry; IránFil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Química Analitica; ArgentinaFil: Skov, Thomas. Universidad de Copenhagen; DinamarcaFil: Mansouri, Kamran. Kermanshah University of Medical Sciences; IránElsevier2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17364Jalalvand, Ali R.; Gholivand, Mohammad Bagher; Goicoechea, Hector Casimiro; Skov, Thomas; Mansouri, Kamran; Mimicking enzymatic effects of cytochrome P450 by an efficient biosensor for in vitro detection of DNA damage; Elsevier; International Journal of Biological Macromolecules; 79; 5-2015; 1004-10100141-8130enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2015.05.047info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0141813015003888info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:39:26Zoai:ri.conicet.gov.ar:11336/17364instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:39:27.118CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mimicking enzymatic effects of cytochrome P450 by an efficient biosensor for in vitro detection of DNA damage
title Mimicking enzymatic effects of cytochrome P450 by an efficient biosensor for in vitro detection of DNA damage
spellingShingle Mimicking enzymatic effects of cytochrome P450 by an efficient biosensor for in vitro detection of DNA damage
Jalalvand, Ali R.
Biosensor
Dna Damage
Benzo(A)Pyrene
title_short Mimicking enzymatic effects of cytochrome P450 by an efficient biosensor for in vitro detection of DNA damage
title_full Mimicking enzymatic effects of cytochrome P450 by an efficient biosensor for in vitro detection of DNA damage
title_fullStr Mimicking enzymatic effects of cytochrome P450 by an efficient biosensor for in vitro detection of DNA damage
title_full_unstemmed Mimicking enzymatic effects of cytochrome P450 by an efficient biosensor for in vitro detection of DNA damage
title_sort Mimicking enzymatic effects of cytochrome P450 by an efficient biosensor for in vitro detection of DNA damage
dc.creator.none.fl_str_mv Jalalvand, Ali R.
Gholivand, Mohammad Bagher
Goicoechea, Hector Casimiro
Skov, Thomas
Mansouri, Kamran
author Jalalvand, Ali R.
author_facet Jalalvand, Ali R.
Gholivand, Mohammad Bagher
Goicoechea, Hector Casimiro
Skov, Thomas
Mansouri, Kamran
author_role author
author2 Gholivand, Mohammad Bagher
Goicoechea, Hector Casimiro
Skov, Thomas
Mansouri, Kamran
author2_role author
author
author
author
dc.subject.none.fl_str_mv Biosensor
Dna Damage
Benzo(A)Pyrene
topic Biosensor
Dna Damage
Benzo(A)Pyrene
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A novel biosensor for detecting DNA damage induced by benzo(a)pyrene (BP) and its metabolite was presented 18 in this work. The nafion-solubilized single wall carbon nanotubes-ionic liquid (SWCNTs-NA-IL) composite film 19 was prepared and then horseradish peroxidase (HRP) and double-stranded DNA were alternately assembled on 20 the composite film by the layer-by-layer method. The biosensor was characterized by cyclic voltammetry (CV), 21 electrochemical impedance spectroscopy (EIS), differential pulse voltammetry (DPV), scanning electron 22 microscopy (SEM) and computational methods. UVvis spectrophotometry was also used to investigate DNA 23 damage induced by BP and its metabolites in solution. The DNA biosensor was treated separately in BP, 24 hydrogen peroxide (H2O2) and in their mixture, respectively. The EIS analysis showed a decrease in the charge 25 transfer resistance at the DNA/HRP/SWCNTs-NA-IL/GCE incubated in a mixture of HRP and H2O2, because 26 HRP in the presence of H2O2 could mimic enzymatic effects of cytochrome P450 (CYP450) to metabolize BP 27 which could cause significant DNA damage and the exposed DNA bases reduced the electrostatic repulsion of the 28 negatively charged redox probe and leads to Faradaic impedance changes. Finally, a novel biosensor for BP 29 determination was developed and this method provided an indirect, and quantitative estimate of DNA damage in 30 vitro.
Fil: Jalalvand, Ali R.. Razi University. Department of Analytical Chemistry; Irán. Kermanshah University of Medical Sciences; Irán. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Química Analitica; Argentina
Fil: Gholivand, Mohammad Bagher. Razi University. Department of Analytical Chemistry; Irán
Fil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Química Analitica; Argentina
Fil: Skov, Thomas. Universidad de Copenhagen; Dinamarca
Fil: Mansouri, Kamran. Kermanshah University of Medical Sciences; Irán
description A novel biosensor for detecting DNA damage induced by benzo(a)pyrene (BP) and its metabolite was presented 18 in this work. The nafion-solubilized single wall carbon nanotubes-ionic liquid (SWCNTs-NA-IL) composite film 19 was prepared and then horseradish peroxidase (HRP) and double-stranded DNA were alternately assembled on 20 the composite film by the layer-by-layer method. The biosensor was characterized by cyclic voltammetry (CV), 21 electrochemical impedance spectroscopy (EIS), differential pulse voltammetry (DPV), scanning electron 22 microscopy (SEM) and computational methods. UVvis spectrophotometry was also used to investigate DNA 23 damage induced by BP and its metabolites in solution. The DNA biosensor was treated separately in BP, 24 hydrogen peroxide (H2O2) and in their mixture, respectively. The EIS analysis showed a decrease in the charge 25 transfer resistance at the DNA/HRP/SWCNTs-NA-IL/GCE incubated in a mixture of HRP and H2O2, because 26 HRP in the presence of H2O2 could mimic enzymatic effects of cytochrome P450 (CYP450) to metabolize BP 27 which could cause significant DNA damage and the exposed DNA bases reduced the electrostatic repulsion of the 28 negatively charged redox probe and leads to Faradaic impedance changes. Finally, a novel biosensor for BP 29 determination was developed and this method provided an indirect, and quantitative estimate of DNA damage in 30 vitro.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/17364
Jalalvand, Ali R.; Gholivand, Mohammad Bagher; Goicoechea, Hector Casimiro; Skov, Thomas; Mansouri, Kamran; Mimicking enzymatic effects of cytochrome P450 by an efficient biosensor for in vitro detection of DNA damage; Elsevier; International Journal of Biological Macromolecules; 79; 5-2015; 1004-1010
0141-8130
url http://hdl.handle.net/11336/17364
identifier_str_mv Jalalvand, Ali R.; Gholivand, Mohammad Bagher; Goicoechea, Hector Casimiro; Skov, Thomas; Mansouri, Kamran; Mimicking enzymatic effects of cytochrome P450 by an efficient biosensor for in vitro detection of DNA damage; Elsevier; International Journal of Biological Macromolecules; 79; 5-2015; 1004-1010
0141-8130
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2015.05.047
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0141813015003888
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082879561400320
score 13.22299

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