Study of genotoxicity and citotoxicity of MccJ25 (G12Y)

Autores
Koltan, Mariano; Bulacio Gil, Natalia María; Rubio Molina, Anna Carolina; Zenoff, Ana Maria; Caram Di Santo, Maria Carolina; de Cristobal, Ricardo Ezequiel; Adler, Conrado; Vincent, Paula Andrea; Pomares, Maria Fernanda
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
MccJ25 (G12Y) is an antimicrobial peptide active against E. coli O157 H: 7 and other diarrheagenic E. coli strains. MccJ25(G12Y) presents advantageous properties as a food preservative: it resists toextreme temperatures, it?s stable in a wide range of pH and exerts antimicrobial activity in food samples (egg yolk and milk). Our long-term aim is to develop a food preservative based on MccJ25 (G12Y).Therefore, we were interested in studying MccJ25 (G12Y) safety for humans. For this, we evaluated MccJ25 (G12Y)?s cytotoxicity on Artemia salina and Galleria mellonella, and its acute toxicity in BALB-cmice. In addition, we performed Ames test in order to test the peptide?s mutagenic potential. Results obtained confirmed the absence of genotoxic effects of MccJ25 (G12Y) on S. typhimurium.Furthermore, the assay performed in Artemia salina revealed that 0.31, 0.62, 1.25, 2.25 and 3 mg/ml of MccJ25 (G12Y) did not significantly affect their viability. In fact, the LD50 was not reached with anyof the MccJ25 (G12Y) concentrations tested. When MccJ25 (G12Y) citotoxicity was evaluated in Galleria mellonella, low toxicity was observed with the studied concentrations (1500 and 2500 mg/kg).Finally, acute toxicity was studied in female BALB-c mice. Neither death nor symptoms of toxicity in animals were observed. In addition, macroscopic pathological studies showed that the organs of MccJ25(G12Y)-treated animals did not present any morphological alteration. Taken together, results indicate that MccJ25 (G12Y) could be safe for humans. This finding is relevant in view of its application as anatural preservative to extend foods? shelf-life.
Fil: Koltan, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Bulacio Gil, Natalia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Rubio Molina, Anna Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Zenoff, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Caram Di Santo, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: de Cristobal, Ricardo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Adler, Conrado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Vincent, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pomares, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
The LV Annual SAIB Meeting and XIV PABMB Congress
Salta
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Panamerican Association of Biochemestry and Molecular Biology
Materia
genotoxicity
citotoxicity
MccJ25 (G12Y)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181768
Acceder
id CONICETDig_554ff03e91538c75d39f963899685a42
oai_identifier_str oai:ri.conicet.gov.ar:11336/181768
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Study of genotoxicity and citotoxicity of MccJ25 (G12Y)Koltan, MarianoBulacio Gil, Natalia MaríaRubio Molina, Anna CarolinaZenoff, Ana MariaCaram Di Santo, Maria Carolinade Cristobal, Ricardo EzequielAdler, ConradoVincent, Paula AndreaPomares, Maria FernandagenotoxicitycitotoxicityMccJ25 (G12Y)https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1MccJ25 (G12Y) is an antimicrobial peptide active against E. coli O157 H: 7 and other diarrheagenic E. coli strains. MccJ25(G12Y) presents advantageous properties as a food preservative: it resists toextreme temperatures, it?s stable in a wide range of pH and exerts antimicrobial activity in food samples (egg yolk and milk). Our long-term aim is to develop a food preservative based on MccJ25 (G12Y).Therefore, we were interested in studying MccJ25 (G12Y) safety for humans. For this, we evaluated MccJ25 (G12Y)?s cytotoxicity on Artemia salina and Galleria mellonella, and its acute toxicity in BALB-cmice. In addition, we performed Ames test in order to test the peptide?s mutagenic potential. Results obtained confirmed the absence of genotoxic effects of MccJ25 (G12Y) on S. typhimurium.Furthermore, the assay performed in Artemia salina revealed that 0.31, 0.62, 1.25, 2.25 and 3 mg/ml of MccJ25 (G12Y) did not significantly affect their viability. In fact, the LD50 was not reached with anyof the MccJ25 (G12Y) concentrations tested. When MccJ25 (G12Y) citotoxicity was evaluated in Galleria mellonella, low toxicity was observed with the studied concentrations (1500 and 2500 mg/kg).Finally, acute toxicity was studied in female BALB-c mice. Neither death nor symptoms of toxicity in animals were observed. In addition, macroscopic pathological studies showed that the organs of MccJ25(G12Y)-treated animals did not present any morphological alteration. Taken together, results indicate that MccJ25 (G12Y) could be safe for humans. This finding is relevant in view of its application as anatural preservative to extend foods? shelf-life.Fil: Koltan, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Bulacio Gil, Natalia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Rubio Molina, Anna Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Zenoff, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Caram Di Santo, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: de Cristobal, Ricardo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Adler, Conrado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Vincent, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Pomares, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaThe LV Annual SAIB Meeting and XIV PABMB CongressSaltaArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularPanamerican Association of Biochemestry and Molecular BiologyTech Science Press2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181768Study of genotoxicity and citotoxicity of MccJ25 (G12Y); The LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 1-61667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://techscience.com/biocell/v43nSuppl.5/33868/pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:49:40Zoai:ri.conicet.gov.ar:11336/181768instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:49:41.011CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Study of genotoxicity and citotoxicity of MccJ25 (G12Y)
title Study of genotoxicity and citotoxicity of MccJ25 (G12Y)
spellingShingle Study of genotoxicity and citotoxicity of MccJ25 (G12Y)
Koltan, Mariano
genotoxicity
citotoxicity
MccJ25 (G12Y)
title_short Study of genotoxicity and citotoxicity of MccJ25 (G12Y)
title_full Study of genotoxicity and citotoxicity of MccJ25 (G12Y)
title_fullStr Study of genotoxicity and citotoxicity of MccJ25 (G12Y)
title_full_unstemmed Study of genotoxicity and citotoxicity of MccJ25 (G12Y)
title_sort Study of genotoxicity and citotoxicity of MccJ25 (G12Y)
dc.creator.none.fl_str_mv Koltan, Mariano
Bulacio Gil, Natalia María
Rubio Molina, Anna Carolina
Zenoff, Ana Maria
Caram Di Santo, Maria Carolina
de Cristobal, Ricardo Ezequiel
Adler, Conrado
Vincent, Paula Andrea
Pomares, Maria Fernanda
author Koltan, Mariano
author_facet Koltan, Mariano
Bulacio Gil, Natalia María
Rubio Molina, Anna Carolina
Zenoff, Ana Maria
Caram Di Santo, Maria Carolina
de Cristobal, Ricardo Ezequiel
Adler, Conrado
Vincent, Paula Andrea
Pomares, Maria Fernanda
author_role author
author2 Bulacio Gil, Natalia María
Rubio Molina, Anna Carolina
Zenoff, Ana Maria
Caram Di Santo, Maria Carolina
de Cristobal, Ricardo Ezequiel
Adler, Conrado
Vincent, Paula Andrea
Pomares, Maria Fernanda
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv genotoxicity
citotoxicity
MccJ25 (G12Y)
topic genotoxicity
citotoxicity
MccJ25 (G12Y)
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv MccJ25 (G12Y) is an antimicrobial peptide active against E. coli O157 H: 7 and other diarrheagenic E. coli strains. MccJ25(G12Y) presents advantageous properties as a food preservative: it resists toextreme temperatures, it?s stable in a wide range of pH and exerts antimicrobial activity in food samples (egg yolk and milk). Our long-term aim is to develop a food preservative based on MccJ25 (G12Y).Therefore, we were interested in studying MccJ25 (G12Y) safety for humans. For this, we evaluated MccJ25 (G12Y)?s cytotoxicity on Artemia salina and Galleria mellonella, and its acute toxicity in BALB-cmice. In addition, we performed Ames test in order to test the peptide?s mutagenic potential. Results obtained confirmed the absence of genotoxic effects of MccJ25 (G12Y) on S. typhimurium.Furthermore, the assay performed in Artemia salina revealed that 0.31, 0.62, 1.25, 2.25 and 3 mg/ml of MccJ25 (G12Y) did not significantly affect their viability. In fact, the LD50 was not reached with anyof the MccJ25 (G12Y) concentrations tested. When MccJ25 (G12Y) citotoxicity was evaluated in Galleria mellonella, low toxicity was observed with the studied concentrations (1500 and 2500 mg/kg).Finally, acute toxicity was studied in female BALB-c mice. Neither death nor symptoms of toxicity in animals were observed. In addition, macroscopic pathological studies showed that the organs of MccJ25(G12Y)-treated animals did not present any morphological alteration. Taken together, results indicate that MccJ25 (G12Y) could be safe for humans. This finding is relevant in view of its application as anatural preservative to extend foods? shelf-life.
Fil: Koltan, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Bulacio Gil, Natalia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Rubio Molina, Anna Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Zenoff, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Caram Di Santo, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: de Cristobal, Ricardo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Adler, Conrado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Vincent, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pomares, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
The LV Annual SAIB Meeting and XIV PABMB Congress
Salta
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Panamerican Association of Biochemestry and Molecular Biology
description MccJ25 (G12Y) is an antimicrobial peptide active against E. coli O157 H: 7 and other diarrheagenic E. coli strains. MccJ25(G12Y) presents advantageous properties as a food preservative: it resists toextreme temperatures, it?s stable in a wide range of pH and exerts antimicrobial activity in food samples (egg yolk and milk). Our long-term aim is to develop a food preservative based on MccJ25 (G12Y).Therefore, we were interested in studying MccJ25 (G12Y) safety for humans. For this, we evaluated MccJ25 (G12Y)?s cytotoxicity on Artemia salina and Galleria mellonella, and its acute toxicity in BALB-cmice. In addition, we performed Ames test in order to test the peptide?s mutagenic potential. Results obtained confirmed the absence of genotoxic effects of MccJ25 (G12Y) on S. typhimurium.Furthermore, the assay performed in Artemia salina revealed that 0.31, 0.62, 1.25, 2.25 and 3 mg/ml of MccJ25 (G12Y) did not significantly affect their viability. In fact, the LD50 was not reached with anyof the MccJ25 (G12Y) concentrations tested. When MccJ25 (G12Y) citotoxicity was evaluated in Galleria mellonella, low toxicity was observed with the studied concentrations (1500 and 2500 mg/kg).Finally, acute toxicity was studied in female BALB-c mice. Neither death nor symptoms of toxicity in animals were observed. In addition, macroscopic pathological studies showed that the organs of MccJ25(G12Y)-treated animals did not present any morphological alteration. Taken together, results indicate that MccJ25 (G12Y) could be safe for humans. This finding is relevant in view of its application as anatural preservative to extend foods? shelf-life.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181768
Study of genotoxicity and citotoxicity of MccJ25 (G12Y); The LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 1-6
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181768
identifier_str_mv Study of genotoxicity and citotoxicity of MccJ25 (G12Y); The LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 1-6
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://techscience.com/biocell/v43nSuppl.5/33868/pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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