Macrophage environment turns otherwise MccJ25-resistant Salmonella into sensitive

Autores
Pomares, Maria Fernanda; Corbalan, Natalia Soledad; Adler, Conrado; de Cristobal, Ricardo Ezequiel; Farias, Ricardo Norberto; Delgado, Monica Alejandra; Vincent, Paula Andrea
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work we demonstrate that S. Typhimurium becomes notably susceptible to MccJ25 when replicating within macrophages. In order to determine the possible cause of this phenomenon, we studied the sensitivity of S. Typhimurium to MccJ25 at conditions resembling those of the internal macrophage environment, such as low pH, low magnesium and iron deprivation. We observed that the strain was only sensitive to the antibiotic at low pH, leading us to attribute the bacterial sensitization to this condition. A MccJ25-resistant E. coli strain in which fhuA is deleted was also inhibited by the antibiotic at low pH. Then, we could assume that the MccJ25 sensitivity change observed in both E. coli fhuA and S. Typhimurium is mediated by a MccJ25 uptake independent of the FhuA receptor. Moreover, low pH incubation also sensitized S. Typhimurium to the hydrophobic antibiotic novobiocin, which does not affect enteric bacteria viability because it is unable to penetrate the bacterial outer membrane. This observation supports our hypothesis about low pH producing a modification in the bacterial membrane permeability that allows an unspecific MccJ25 uptake. On the other hand, MccJ25 inhibited S. Typhimurium when cells were preincubated in acidic pH medium and then treated at neutral pH with the antibiotic.
Fil: Pomares, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;
Fil: Corbalan, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;
Fil: Adler, Conrado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;
Fil: de Cristobal, Ricardo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;
Fil: Farias, Ricardo Norberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;
Fil: Delgado, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;
Fil: Vincent, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;
Materia
MICROCIN
SALMONELLA
TARGET
CELL LINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/620
Acceder
id CONICETDig_733e3145cd1378632a3c8c09a84e452c
oai_identifier_str oai:ri.conicet.gov.ar:11336/620
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Macrophage environment turns otherwise MccJ25-resistant Salmonella into sensitivePomares, Maria FernandaCorbalan, Natalia SoledadAdler, Conradode Cristobal, Ricardo EzequielFarias, Ricardo NorbertoDelgado, Monica AlejandraVincent, Paula AndreaMICROCINSALMONELLATARGETCELL LINEhttps://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6In this work we demonstrate that S. Typhimurium becomes notably susceptible to MccJ25 when replicating within macrophages. In order to determine the possible cause of this phenomenon, we studied the sensitivity of S. Typhimurium to MccJ25 at conditions resembling those of the internal macrophage environment, such as low pH, low magnesium and iron deprivation. We observed that the strain was only sensitive to the antibiotic at low pH, leading us to attribute the bacterial sensitization to this condition. A MccJ25-resistant E. coli strain in which fhuA is deleted was also inhibited by the antibiotic at low pH. Then, we could assume that the MccJ25 sensitivity change observed in both E. coli fhuA and S. Typhimurium is mediated by a MccJ25 uptake independent of the FhuA receptor. Moreover, low pH incubation also sensitized S. Typhimurium to the hydrophobic antibiotic novobiocin, which does not affect enteric bacteria viability because it is unable to penetrate the bacterial outer membrane. This observation supports our hypothesis about low pH producing a modification in the bacterial membrane permeability that allows an unspecific MccJ25 uptake. On the other hand, MccJ25 inhibited S. Typhimurium when cells were preincubated in acidic pH medium and then treated at neutral pH with the antibiotic.Fil: Pomares, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;Fil: Corbalan, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;Fil: Adler, Conrado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;Fil: de Cristobal, Ricardo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;Fil: Farias, Ricardo Norberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;Fil: Delgado, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;Fil: Vincent, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;Biomed Central Ltd2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/620Pomares, Maria Fernanda; Corbalan, Natalia Soledad; Adler, Conrado; de Cristobal, Ricardo Ezequiel; Farias, Ricardo Norberto; Delgado, Monica Alejandra; Vincent, Paula Andrea; Macrophage environment turns otherwise MccJ25-resistant Salmonella into sensitive; Biomed Central Ltd; Bmc Microbiology; 13; 5-2013; 1-6;1471-2180enginfo:eu-repo/semantics/altIdentifier/url/http://www.biomedcentral.com/1471-2180/13/95info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:19:38Zoai:ri.conicet.gov.ar:11336/620instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:19:38.731CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Macrophage environment turns otherwise MccJ25-resistant Salmonella into sensitive
title Macrophage environment turns otherwise MccJ25-resistant Salmonella into sensitive
spellingShingle Macrophage environment turns otherwise MccJ25-resistant Salmonella into sensitive
Pomares, Maria Fernanda
MICROCIN
SALMONELLA
TARGET
CELL LINE
title_short Macrophage environment turns otherwise MccJ25-resistant Salmonella into sensitive
title_full Macrophage environment turns otherwise MccJ25-resistant Salmonella into sensitive
title_fullStr Macrophage environment turns otherwise MccJ25-resistant Salmonella into sensitive
title_full_unstemmed Macrophage environment turns otherwise MccJ25-resistant Salmonella into sensitive
title_sort Macrophage environment turns otherwise MccJ25-resistant Salmonella into sensitive
dc.creator.none.fl_str_mv Pomares, Maria Fernanda
Corbalan, Natalia Soledad
Adler, Conrado
de Cristobal, Ricardo Ezequiel
Farias, Ricardo Norberto
Delgado, Monica Alejandra
Vincent, Paula Andrea
author Pomares, Maria Fernanda
author_facet Pomares, Maria Fernanda
Corbalan, Natalia Soledad
Adler, Conrado
de Cristobal, Ricardo Ezequiel
Farias, Ricardo Norberto
Delgado, Monica Alejandra
Vincent, Paula Andrea
author_role author
author2 Corbalan, Natalia Soledad
Adler, Conrado
de Cristobal, Ricardo Ezequiel
Farias, Ricardo Norberto
Delgado, Monica Alejandra
Vincent, Paula Andrea
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv MICROCIN
SALMONELLA
TARGET
CELL LINE
topic MICROCIN
SALMONELLA
TARGET
CELL LINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
dc.description.none.fl_txt_mv In this work we demonstrate that S. Typhimurium becomes notably susceptible to MccJ25 when replicating within macrophages. In order to determine the possible cause of this phenomenon, we studied the sensitivity of S. Typhimurium to MccJ25 at conditions resembling those of the internal macrophage environment, such as low pH, low magnesium and iron deprivation. We observed that the strain was only sensitive to the antibiotic at low pH, leading us to attribute the bacterial sensitization to this condition. A MccJ25-resistant E. coli strain in which fhuA is deleted was also inhibited by the antibiotic at low pH. Then, we could assume that the MccJ25 sensitivity change observed in both E. coli fhuA and S. Typhimurium is mediated by a MccJ25 uptake independent of the FhuA receptor. Moreover, low pH incubation also sensitized S. Typhimurium to the hydrophobic antibiotic novobiocin, which does not affect enteric bacteria viability because it is unable to penetrate the bacterial outer membrane. This observation supports our hypothesis about low pH producing a modification in the bacterial membrane permeability that allows an unspecific MccJ25 uptake. On the other hand, MccJ25 inhibited S. Typhimurium when cells were preincubated in acidic pH medium and then treated at neutral pH with the antibiotic.
Fil: Pomares, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;
Fil: Corbalan, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;
Fil: Adler, Conrado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;
Fil: de Cristobal, Ricardo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;
Fil: Farias, Ricardo Norberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;
Fil: Delgado, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;
Fil: Vincent, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucuman. Instituto Superior de Investigaciones Biológicas; Argentina;
description In this work we demonstrate that S. Typhimurium becomes notably susceptible to MccJ25 when replicating within macrophages. In order to determine the possible cause of this phenomenon, we studied the sensitivity of S. Typhimurium to MccJ25 at conditions resembling those of the internal macrophage environment, such as low pH, low magnesium and iron deprivation. We observed that the strain was only sensitive to the antibiotic at low pH, leading us to attribute the bacterial sensitization to this condition. A MccJ25-resistant E. coli strain in which fhuA is deleted was also inhibited by the antibiotic at low pH. Then, we could assume that the MccJ25 sensitivity change observed in both E. coli fhuA and S. Typhimurium is mediated by a MccJ25 uptake independent of the FhuA receptor. Moreover, low pH incubation also sensitized S. Typhimurium to the hydrophobic antibiotic novobiocin, which does not affect enteric bacteria viability because it is unable to penetrate the bacterial outer membrane. This observation supports our hypothesis about low pH producing a modification in the bacterial membrane permeability that allows an unspecific MccJ25 uptake. On the other hand, MccJ25 inhibited S. Typhimurium when cells were preincubated in acidic pH medium and then treated at neutral pH with the antibiotic.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/620
Pomares, Maria Fernanda; Corbalan, Natalia Soledad; Adler, Conrado; de Cristobal, Ricardo Ezequiel; Farias, Ricardo Norberto; Delgado, Monica Alejandra; Vincent, Paula Andrea; Macrophage environment turns otherwise MccJ25-resistant Salmonella into sensitive; Biomed Central Ltd; Bmc Microbiology; 13; 5-2013; 1-6;
1471-2180
url http://hdl.handle.net/11336/620
identifier_str_mv Pomares, Maria Fernanda; Corbalan, Natalia Soledad; Adler, Conrado; de Cristobal, Ricardo Ezequiel; Farias, Ricardo Norberto; Delgado, Monica Alejandra; Vincent, Paula Andrea; Macrophage environment turns otherwise MccJ25-resistant Salmonella into sensitive; Biomed Central Ltd; Bmc Microbiology; 13; 5-2013; 1-6;
1471-2180
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.biomedcentral.com/1471-2180/13/95
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Biomed Central Ltd
publisher.none.fl_str_mv Biomed Central Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614169282740224
score 13.070432

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