MccJ25 C-terminal is involved in RNA-polymerase inhibition but not in respiration inhibition

Autores
Vincent, Paula Andrea; Bellomio, Augusto; Fernandez, Beatriz Eugenia; Farias, Ricardo Norberto; Morero, Roberto Dionisio
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Microcin J25 appears to have two intracellular targets: (1) RNA polymerase, which was described in Escherichia coli and Salmonella enterica serovars, and (2) cell respiration in Salmonella enterica serovars. C-terminal glycine amidation of the threaded segment localized in the MccJ25 lariat ring region specifically blocked the RNA-polymerase inhibition, but not the cell respiration inhibition and peptide uptake. These results suggest that different regions of the molecule are responsible for each cellular effect, they are localized far away from the β-hairpin region and the C-terminal region is an important determinant for RNAP inhibition.
Fil: Vincent, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Bellomio, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Fernandez, Beatriz Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Farias, Ricardo Norberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Morero, Roberto Dionisio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
MCCJ25 AMIDATED DERIVATIVE
MICROCIN J25
OXYGEN CONSUMPTION
RNA POLYMERASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95126
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling MccJ25 C-terminal is involved in RNA-polymerase inhibition but not in respiration inhibitionVincent, Paula AndreaBellomio, AugustoFernandez, Beatriz EugeniaFarias, Ricardo NorbertoMorero, Roberto DionisioMCCJ25 AMIDATED DERIVATIVEMICROCIN J25OXYGEN CONSUMPTIONRNA POLYMERASEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Microcin J25 appears to have two intracellular targets: (1) RNA polymerase, which was described in Escherichia coli and Salmonella enterica serovars, and (2) cell respiration in Salmonella enterica serovars. C-terminal glycine amidation of the threaded segment localized in the MccJ25 lariat ring region specifically blocked the RNA-polymerase inhibition, but not the cell respiration inhibition and peptide uptake. These results suggest that different regions of the molecule are responsible for each cellular effect, they are localized far away from the β-hairpin region and the C-terminal region is an important determinant for RNAP inhibition.Fil: Vincent, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Bellomio, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Fernandez, Beatriz Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Farias, Ricardo Norberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Morero, Roberto Dionisio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaAcademic Press Inc Elsevier Science2005-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95126Vincent, Paula Andrea; Bellomio, Augusto; Fernandez, Beatriz Eugenia; Farias, Ricardo Norberto; Morero, Roberto Dionisio; MccJ25 C-terminal is involved in RNA-polymerase inhibition but not in respiration inhibition; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 331; 2; 6-2005; 549-5510006-291XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2005.03.220info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006291X05007394info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:19Zoai:ri.conicet.gov.ar:11336/95126instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:20.039CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv MccJ25 C-terminal is involved in RNA-polymerase inhibition but not in respiration inhibition
title MccJ25 C-terminal is involved in RNA-polymerase inhibition but not in respiration inhibition
spellingShingle MccJ25 C-terminal is involved in RNA-polymerase inhibition but not in respiration inhibition
Vincent, Paula Andrea
MCCJ25 AMIDATED DERIVATIVE
MICROCIN J25
OXYGEN CONSUMPTION
RNA POLYMERASE
title_short MccJ25 C-terminal is involved in RNA-polymerase inhibition but not in respiration inhibition
title_full MccJ25 C-terminal is involved in RNA-polymerase inhibition but not in respiration inhibition
title_fullStr MccJ25 C-terminal is involved in RNA-polymerase inhibition but not in respiration inhibition
title_full_unstemmed MccJ25 C-terminal is involved in RNA-polymerase inhibition but not in respiration inhibition
title_sort MccJ25 C-terminal is involved in RNA-polymerase inhibition but not in respiration inhibition
dc.creator.none.fl_str_mv Vincent, Paula Andrea
Bellomio, Augusto
Fernandez, Beatriz Eugenia
Farias, Ricardo Norberto
Morero, Roberto Dionisio
author Vincent, Paula Andrea
author_facet Vincent, Paula Andrea
Bellomio, Augusto
Fernandez, Beatriz Eugenia
Farias, Ricardo Norberto
Morero, Roberto Dionisio
author_role author
author2 Bellomio, Augusto
Fernandez, Beatriz Eugenia
Farias, Ricardo Norberto
Morero, Roberto Dionisio
author2_role author
author
author
author
dc.subject.none.fl_str_mv MCCJ25 AMIDATED DERIVATIVE
MICROCIN J25
OXYGEN CONSUMPTION
RNA POLYMERASE
topic MCCJ25 AMIDATED DERIVATIVE
MICROCIN J25
OXYGEN CONSUMPTION
RNA POLYMERASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Microcin J25 appears to have two intracellular targets: (1) RNA polymerase, which was described in Escherichia coli and Salmonella enterica serovars, and (2) cell respiration in Salmonella enterica serovars. C-terminal glycine amidation of the threaded segment localized in the MccJ25 lariat ring region specifically blocked the RNA-polymerase inhibition, but not the cell respiration inhibition and peptide uptake. These results suggest that different regions of the molecule are responsible for each cellular effect, they are localized far away from the β-hairpin region and the C-terminal region is an important determinant for RNAP inhibition.
Fil: Vincent, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Bellomio, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Fernandez, Beatriz Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Farias, Ricardo Norberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Morero, Roberto Dionisio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description Microcin J25 appears to have two intracellular targets: (1) RNA polymerase, which was described in Escherichia coli and Salmonella enterica serovars, and (2) cell respiration in Salmonella enterica serovars. C-terminal glycine amidation of the threaded segment localized in the MccJ25 lariat ring region specifically blocked the RNA-polymerase inhibition, but not the cell respiration inhibition and peptide uptake. These results suggest that different regions of the molecule are responsible for each cellular effect, they are localized far away from the β-hairpin region and the C-terminal region is an important determinant for RNAP inhibition.
publishDate 2005
dc.date.none.fl_str_mv 2005-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95126
Vincent, Paula Andrea; Bellomio, Augusto; Fernandez, Beatriz Eugenia; Farias, Ricardo Norberto; Morero, Roberto Dionisio; MccJ25 C-terminal is involved in RNA-polymerase inhibition but not in respiration inhibition; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 331; 2; 6-2005; 549-551
0006-291X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95126
identifier_str_mv Vincent, Paula Andrea; Bellomio, Augusto; Fernandez, Beatriz Eugenia; Farias, Ricardo Norberto; Morero, Roberto Dionisio; MccJ25 C-terminal is involved in RNA-polymerase inhibition but not in respiration inhibition; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 331; 2; 6-2005; 549-551
0006-291X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2005.03.220
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006291X05007394
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613210911539200
score 13.070432

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