UV-B inhibits leaf growth through changes in growth regulating factors and Gibberellin levels

Autores
Fina, Julieta Paola; Casadevall, Romina; Abdelgawad, Hamada; Prinsen, Els; Markakis, Marios N.; Beemster, Gerrit T. S.; Casati, Paula
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ultraviolet-B (UV-B) radiation affects leaf growth in a wide range of species. In this work, we demonstrate that UV-B levels present in solar radiation inhibit maize (Zea mays) leaf growth without causing any other visible stress symptoms, including the accumulation of DNA damage. We conducted kinematic analyses of cell division and expansion to understand the impact of UV-B radiation on these cellular processes. Our results demonstrate that the decrease in leaf growth in UV-B-irradiated leaves is a consequence of a reduction in cell production and a shortened growth zone (GZ). To determine the molecular pathways involved in UV-B inhibition of leaf growth, we performed RNA sequencing on isolated GZ tissues of control and UV-B-exposed plants. Our results show a link between the observed leaf growth inhibition and the expression of specific cell cycle and developmental genes, including growth-regulating factors (GRFs) and transcripts for proteins participating in different hormone pathways. Interestingly, the decrease in the GZ size correlates with a decrease in the concentration of GA19, the immediate precursor of the active gibberellin, GA1, by UV-B in this zone, which is regulated, at least in part, by the expression of GRF1 and possibly other transcription factors of the GRF family.
Fil: Fina, Julieta Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Casadevall, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Abdelgawad, Hamada. Universiteit Antwerp; Bélgica. University of Beni-Suef; Egipto
Fil: Prinsen, Els. Universiteit Antwerp; Bélgica
Fil: Markakis, Marios N.. Universiteit Antwerp; Bélgica
Fil: Beemster, Gerrit T. S.. Universiteit Antwerp; Bélgica
Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Materia
UV-B
maize
Growth Regulating Factors
Gibberellin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53197
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/53197
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling UV-B inhibits leaf growth through changes in growth regulating factors and Gibberellin levelsFina, Julieta PaolaCasadevall, RominaAbdelgawad, HamadaPrinsen, ElsMarkakis, Marios N.Beemster, Gerrit T. S.Casati, PaulaUV-BmaizeGrowth Regulating FactorsGibberellinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Ultraviolet-B (UV-B) radiation affects leaf growth in a wide range of species. In this work, we demonstrate that UV-B levels present in solar radiation inhibit maize (Zea mays) leaf growth without causing any other visible stress symptoms, including the accumulation of DNA damage. We conducted kinematic analyses of cell division and expansion to understand the impact of UV-B radiation on these cellular processes. Our results demonstrate that the decrease in leaf growth in UV-B-irradiated leaves is a consequence of a reduction in cell production and a shortened growth zone (GZ). To determine the molecular pathways involved in UV-B inhibition of leaf growth, we performed RNA sequencing on isolated GZ tissues of control and UV-B-exposed plants. Our results show a link between the observed leaf growth inhibition and the expression of specific cell cycle and developmental genes, including growth-regulating factors (GRFs) and transcripts for proteins participating in different hormone pathways. Interestingly, the decrease in the GZ size correlates with a decrease in the concentration of GA19, the immediate precursor of the active gibberellin, GA1, by UV-B in this zone, which is regulated, at least in part, by the expression of GRF1 and possibly other transcription factors of the GRF family.Fil: Fina, Julieta Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Casadevall, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Abdelgawad, Hamada. Universiteit Antwerp; Bélgica. University of Beni-Suef; EgiptoFil: Prinsen, Els. Universiteit Antwerp; BélgicaFil: Markakis, Marios N.. Universiteit Antwerp; BélgicaFil: Beemster, Gerrit T. S.. Universiteit Antwerp; BélgicaFil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaAmerican Society of Plant Biologist2017-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53197Fina, Julieta Paola; Casadevall, Romina; Abdelgawad, Hamada; Prinsen, Els; Markakis, Marios N.; et al.; UV-B inhibits leaf growth through changes in growth regulating factors and Gibberellin levels; American Society of Plant Biologist; Plant Physiology; 174; 2; 6-2017; 1110-11260032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1104/pp.17.00365info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/174/2/1110info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:16Zoai:ri.conicet.gov.ar:11336/53197instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:17.212CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv UV-B inhibits leaf growth through changes in growth regulating factors and Gibberellin levels
title UV-B inhibits leaf growth through changes in growth regulating factors and Gibberellin levels
spellingShingle UV-B inhibits leaf growth through changes in growth regulating factors and Gibberellin levels
Fina, Julieta Paola
UV-B
maize
Growth Regulating Factors
Gibberellin
title_short UV-B inhibits leaf growth through changes in growth regulating factors and Gibberellin levels
title_full UV-B inhibits leaf growth through changes in growth regulating factors and Gibberellin levels
title_fullStr UV-B inhibits leaf growth through changes in growth regulating factors and Gibberellin levels
title_full_unstemmed UV-B inhibits leaf growth through changes in growth regulating factors and Gibberellin levels
title_sort UV-B inhibits leaf growth through changes in growth regulating factors and Gibberellin levels
dc.creator.none.fl_str_mv Fina, Julieta Paola
Casadevall, Romina
Abdelgawad, Hamada
Prinsen, Els
Markakis, Marios N.
Beemster, Gerrit T. S.
Casati, Paula
author Fina, Julieta Paola
author_facet Fina, Julieta Paola
Casadevall, Romina
Abdelgawad, Hamada
Prinsen, Els
Markakis, Marios N.
Beemster, Gerrit T. S.
Casati, Paula
author_role author
author2 Casadevall, Romina
Abdelgawad, Hamada
Prinsen, Els
Markakis, Marios N.
Beemster, Gerrit T. S.
Casati, Paula
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv UV-B
maize
Growth Regulating Factors
Gibberellin
topic UV-B
maize
Growth Regulating Factors
Gibberellin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Ultraviolet-B (UV-B) radiation affects leaf growth in a wide range of species. In this work, we demonstrate that UV-B levels present in solar radiation inhibit maize (Zea mays) leaf growth without causing any other visible stress symptoms, including the accumulation of DNA damage. We conducted kinematic analyses of cell division and expansion to understand the impact of UV-B radiation on these cellular processes. Our results demonstrate that the decrease in leaf growth in UV-B-irradiated leaves is a consequence of a reduction in cell production and a shortened growth zone (GZ). To determine the molecular pathways involved in UV-B inhibition of leaf growth, we performed RNA sequencing on isolated GZ tissues of control and UV-B-exposed plants. Our results show a link between the observed leaf growth inhibition and the expression of specific cell cycle and developmental genes, including growth-regulating factors (GRFs) and transcripts for proteins participating in different hormone pathways. Interestingly, the decrease in the GZ size correlates with a decrease in the concentration of GA19, the immediate precursor of the active gibberellin, GA1, by UV-B in this zone, which is regulated, at least in part, by the expression of GRF1 and possibly other transcription factors of the GRF family.
Fil: Fina, Julieta Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Casadevall, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Abdelgawad, Hamada. Universiteit Antwerp; Bélgica. University of Beni-Suef; Egipto
Fil: Prinsen, Els. Universiteit Antwerp; Bélgica
Fil: Markakis, Marios N.. Universiteit Antwerp; Bélgica
Fil: Beemster, Gerrit T. S.. Universiteit Antwerp; Bélgica
Fil: Casati, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
description Ultraviolet-B (UV-B) radiation affects leaf growth in a wide range of species. In this work, we demonstrate that UV-B levels present in solar radiation inhibit maize (Zea mays) leaf growth without causing any other visible stress symptoms, including the accumulation of DNA damage. We conducted kinematic analyses of cell division and expansion to understand the impact of UV-B radiation on these cellular processes. Our results demonstrate that the decrease in leaf growth in UV-B-irradiated leaves is a consequence of a reduction in cell production and a shortened growth zone (GZ). To determine the molecular pathways involved in UV-B inhibition of leaf growth, we performed RNA sequencing on isolated GZ tissues of control and UV-B-exposed plants. Our results show a link between the observed leaf growth inhibition and the expression of specific cell cycle and developmental genes, including growth-regulating factors (GRFs) and transcripts for proteins participating in different hormone pathways. Interestingly, the decrease in the GZ size correlates with a decrease in the concentration of GA19, the immediate precursor of the active gibberellin, GA1, by UV-B in this zone, which is regulated, at least in part, by the expression of GRF1 and possibly other transcription factors of the GRF family.
publishDate 2017
dc.date.none.fl_str_mv 2017-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53197
Fina, Julieta Paola; Casadevall, Romina; Abdelgawad, Hamada; Prinsen, Els; Markakis, Marios N.; et al.; UV-B inhibits leaf growth through changes in growth regulating factors and Gibberellin levels; American Society of Plant Biologist; Plant Physiology; 174; 2; 6-2017; 1110-1126
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53197
identifier_str_mv Fina, Julieta Paola; Casadevall, Romina; Abdelgawad, Hamada; Prinsen, Els; Markakis, Marios N.; et al.; UV-B inhibits leaf growth through changes in growth regulating factors and Gibberellin levels; American Society of Plant Biologist; Plant Physiology; 174; 2; 6-2017; 1110-1126
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.17.00365
info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/content/174/2/1110
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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