Induction of gibberellin 20-oxidases and repression of gibberellin 2β-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and par...

Autores
Mignolli, Francesco; Vidoz, María Laura; Mariotti, Lorenzo; Lombardi, Lara; Picciarelli, Piero
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In tomato (Solanum lycopersicum L.), auxin and gibberellins (GAs) cross-talk plays an important role during fruit-set. The entire tomato mutant has been previously reported to carry a deletion in the coding region of the SlIAA9 gene, a member of the auxin signal repressor family Aux/IAA. In this paper, we examined the role of ENTIRE gene in controlling GAs metabolism and directing spontaneous fruit initiation and early ovary growth. It was shown that, similarly to pollinated fruits, facultative parthenocarpy in entire depends on active GA metabolism, since fruit growth is suppressed when GA biosynthesis is blocked. Analysis of endogenous GAs during the first 10 days after flower emasculation revealed that entire fruits accumulated higher amounts of active GAs (GA1 and GA3) in comparison to wild type pollinated fruits, suggesting that a different GA homeostasis regulation occurs. Transcript analysis of the main GA biosynthesis genes showed that differently from unpollinated and non parthenocarpic wild type ovaries, in entire active GA flux modulation is regulated by the activation of SlGA20ox1 and SlGA20ox2 and also by a marked reduction of GA catabolism (reduced transcription of GA 2β-oxidase genes) during the early fruit expansion phase.
Fil: Mignolli, Francesco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Vidoz, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Mariotti, Lorenzo. Università degli Studi di Pisa; Italia
Fil: Lombardi, Lara. Università degli Studi di Pisa; Italia
Fil: Picciarelli, Piero. Università degli Studi di Pisa; Italia
Materia
ENTIRE MUTANT
FRUIT DEVELOPMENT
GIBBERELLIN METABOLISM
PARTHENOCARPY
SLIAA9
TOMATO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/183213
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/183213
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Induction of gibberellin 20-oxidases and repression of gibberellin 2β-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formationMignolli, FrancescoVidoz, María LauraMariotti, LorenzoLombardi, LaraPicciarelli, PieroENTIRE MUTANTFRUIT DEVELOPMENTGIBBERELLIN METABOLISMPARTHENOCARPYSLIAA9TOMATOhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4In tomato (Solanum lycopersicum L.), auxin and gibberellins (GAs) cross-talk plays an important role during fruit-set. The entire tomato mutant has been previously reported to carry a deletion in the coding region of the SlIAA9 gene, a member of the auxin signal repressor family Aux/IAA. In this paper, we examined the role of ENTIRE gene in controlling GAs metabolism and directing spontaneous fruit initiation and early ovary growth. It was shown that, similarly to pollinated fruits, facultative parthenocarpy in entire depends on active GA metabolism, since fruit growth is suppressed when GA biosynthesis is blocked. Analysis of endogenous GAs during the first 10 days after flower emasculation revealed that entire fruits accumulated higher amounts of active GAs (GA1 and GA3) in comparison to wild type pollinated fruits, suggesting that a different GA homeostasis regulation occurs. Transcript analysis of the main GA biosynthesis genes showed that differently from unpollinated and non parthenocarpic wild type ovaries, in entire active GA flux modulation is regulated by the activation of SlGA20ox1 and SlGA20ox2 and also by a marked reduction of GA catabolism (reduced transcription of GA 2β-oxidase genes) during the early fruit expansion phase.Fil: Mignolli, Francesco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Vidoz, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaFil: Mariotti, Lorenzo. Università degli Studi di Pisa; ItaliaFil: Lombardi, Lara. Università degli Studi di Pisa; ItaliaFil: Picciarelli, Piero. Università degli Studi di Pisa; ItaliaSpringer2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/183213Mignolli, Francesco; Vidoz, María Laura; Mariotti, Lorenzo; Lombardi, Lara; Picciarelli, Piero; Induction of gibberellin 20-oxidases and repression of gibberellin 2β-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation; Springer; Plant Growth Regulation; 75; 2; 12-2014; 415-4250167-6903CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s10725-014-0002-1info:eu-repo/semantics/altIdentifier/doi/10.1007/s10725-014-0002-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:38Zoai:ri.conicet.gov.ar:11336/183213instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:39.16CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Induction of gibberellin 20-oxidases and repression of gibberellin 2β-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation
title Induction of gibberellin 20-oxidases and repression of gibberellin 2β-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation
spellingShingle Induction of gibberellin 20-oxidases and repression of gibberellin 2β-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation
Mignolli, Francesco
ENTIRE MUTANT
FRUIT DEVELOPMENT
GIBBERELLIN METABOLISM
PARTHENOCARPY
SLIAA9
TOMATO
title_short Induction of gibberellin 20-oxidases and repression of gibberellin 2β-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation
title_full Induction of gibberellin 20-oxidases and repression of gibberellin 2β-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation
title_fullStr Induction of gibberellin 20-oxidases and repression of gibberellin 2β-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation
title_full_unstemmed Induction of gibberellin 20-oxidases and repression of gibberellin 2β-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation
title_sort Induction of gibberellin 20-oxidases and repression of gibberellin 2β-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation
dc.creator.none.fl_str_mv Mignolli, Francesco
Vidoz, María Laura
Mariotti, Lorenzo
Lombardi, Lara
Picciarelli, Piero
author Mignolli, Francesco
author_facet Mignolli, Francesco
Vidoz, María Laura
Mariotti, Lorenzo
Lombardi, Lara
Picciarelli, Piero
author_role author
author2 Vidoz, María Laura
Mariotti, Lorenzo
Lombardi, Lara
Picciarelli, Piero
author2_role author
author
author
author
dc.subject.none.fl_str_mv ENTIRE MUTANT
FRUIT DEVELOPMENT
GIBBERELLIN METABOLISM
PARTHENOCARPY
SLIAA9
TOMATO
topic ENTIRE MUTANT
FRUIT DEVELOPMENT
GIBBERELLIN METABOLISM
PARTHENOCARPY
SLIAA9
TOMATO
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In tomato (Solanum lycopersicum L.), auxin and gibberellins (GAs) cross-talk plays an important role during fruit-set. The entire tomato mutant has been previously reported to carry a deletion in the coding region of the SlIAA9 gene, a member of the auxin signal repressor family Aux/IAA. In this paper, we examined the role of ENTIRE gene in controlling GAs metabolism and directing spontaneous fruit initiation and early ovary growth. It was shown that, similarly to pollinated fruits, facultative parthenocarpy in entire depends on active GA metabolism, since fruit growth is suppressed when GA biosynthesis is blocked. Analysis of endogenous GAs during the first 10 days after flower emasculation revealed that entire fruits accumulated higher amounts of active GAs (GA1 and GA3) in comparison to wild type pollinated fruits, suggesting that a different GA homeostasis regulation occurs. Transcript analysis of the main GA biosynthesis genes showed that differently from unpollinated and non parthenocarpic wild type ovaries, in entire active GA flux modulation is regulated by the activation of SlGA20ox1 and SlGA20ox2 and also by a marked reduction of GA catabolism (reduced transcription of GA 2β-oxidase genes) during the early fruit expansion phase.
Fil: Mignolli, Francesco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Vidoz, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Mariotti, Lorenzo. Università degli Studi di Pisa; Italia
Fil: Lombardi, Lara. Università degli Studi di Pisa; Italia
Fil: Picciarelli, Piero. Università degli Studi di Pisa; Italia
description In tomato (Solanum lycopersicum L.), auxin and gibberellins (GAs) cross-talk plays an important role during fruit-set. The entire tomato mutant has been previously reported to carry a deletion in the coding region of the SlIAA9 gene, a member of the auxin signal repressor family Aux/IAA. In this paper, we examined the role of ENTIRE gene in controlling GAs metabolism and directing spontaneous fruit initiation and early ovary growth. It was shown that, similarly to pollinated fruits, facultative parthenocarpy in entire depends on active GA metabolism, since fruit growth is suppressed when GA biosynthesis is blocked. Analysis of endogenous GAs during the first 10 days after flower emasculation revealed that entire fruits accumulated higher amounts of active GAs (GA1 and GA3) in comparison to wild type pollinated fruits, suggesting that a different GA homeostasis regulation occurs. Transcript analysis of the main GA biosynthesis genes showed that differently from unpollinated and non parthenocarpic wild type ovaries, in entire active GA flux modulation is regulated by the activation of SlGA20ox1 and SlGA20ox2 and also by a marked reduction of GA catabolism (reduced transcription of GA 2β-oxidase genes) during the early fruit expansion phase.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/183213
Mignolli, Francesco; Vidoz, María Laura; Mariotti, Lorenzo; Lombardi, Lara; Picciarelli, Piero; Induction of gibberellin 20-oxidases and repression of gibberellin 2β-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation; Springer; Plant Growth Regulation; 75; 2; 12-2014; 415-425
0167-6903
CONICET Digital
CONICET
url http://hdl.handle.net/11336/183213
identifier_str_mv Mignolli, Francesco; Vidoz, María Laura; Mariotti, Lorenzo; Lombardi, Lara; Picciarelli, Piero; Induction of gibberellin 20-oxidases and repression of gibberellin 2β-oxidases in unfertilized ovaries of entire tomato mutant, leads to accumulation of active gibberellins and parthenocarpic fruit formation; Springer; Plant Growth Regulation; 75; 2; 12-2014; 415-425
0167-6903
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s10725-014-0002-1
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10725-014-0002-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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