Droplets for Gene Editing Using CRISPR-Cas9 and Clonal Selection Improvement Using Hydrogels

Autores
Pérez Sosa, Camilo José; Perez, Maximiliano Sebastian; Vallejo Janeta, Alexander Paolo; Bhansali, Shekhar; Miriuka, Santiago Gabriel; Lerner, Betiana
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Gene editing tools have triggered a revolutionary transformation in the realms of cellularand molecular physiology, serving as a fundamental cornerstone for the evolution of disease modelsand assays in cell culture reactions, marked by various enhancements. Concurrently, microfluidicshas emerged over recent decades as a versatile technology capable of elevating performance andreducing costs in daily experiments across diverse scientific disciplines, with a pronounced impacton cell biology. The amalgamation of these groundbreaking techniques holds the potential to amplifythe generation of stable cell lines and the production of extracellular matrix hydrogels. Thesehydrogels, assuming a pivotal role in isolating cells at the single-cell level, facilitate a myriad ofanalyses. This study presents a novel method that seamlessly integrates CRISPR-Cas9 gene editingtechniques with single-cell isolation methods in induced pluripotent stem cell (hiPSC) lines, utilizingthe combined power of droplets and hydrogels. This innovative approach is designed to optimizeclonal selection, thereby concurrently reducing costs and the time required for generating a stablegenetically modified cell line. By bridging the advancements in gene editing and microfluidictechnologies, our approach not only holds significant promise for the development of disease modelsand assays but also addresses the crucial need for efficient single-cell isolation. This integrationcontributes to streamlining processes, making it a transformative method with implications forenhancing the efficiency and cost-effectiveness of stable cell line generation. As we navigate theintersection of gene editing and microfluidics, our study marks a significant stride toward innovativemethodologies in the dynamic landscape of cellular and molecular physiology research.
Fil: Pérez Sosa, Camilo José. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez, Maximiliano Sebastian. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vallejo Janeta, Alexander Paolo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina
Fil: Bhansali, Shekhar. Florida International University; Estados Unidos
Fil: Miriuka, Santiago Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia; Argentina
Fil: Lerner, Betiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina
Materia
MICROFLUIDICS
SINGLE CELL
DROPLETS
CRISPR-Cas9
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/238433
Acceder
id CONICETDig_54ad03fbbb605a8df8f52c01fae90bb2
oai_identifier_str oai:ri.conicet.gov.ar:11336/238433
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Droplets for Gene Editing Using CRISPR-Cas9 and Clonal Selection Improvement Using HydrogelsPérez Sosa, Camilo JoséPerez, Maximiliano SebastianVallejo Janeta, Alexander PaoloBhansali, ShekharMiriuka, Santiago GabrielLerner, BetianaMICROFLUIDICSSINGLE CELLDROPLETSCRISPR-Cas9https://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Gene editing tools have triggered a revolutionary transformation in the realms of cellularand molecular physiology, serving as a fundamental cornerstone for the evolution of disease modelsand assays in cell culture reactions, marked by various enhancements. Concurrently, microfluidicshas emerged over recent decades as a versatile technology capable of elevating performance andreducing costs in daily experiments across diverse scientific disciplines, with a pronounced impacton cell biology. The amalgamation of these groundbreaking techniques holds the potential to amplifythe generation of stable cell lines and the production of extracellular matrix hydrogels. Thesehydrogels, assuming a pivotal role in isolating cells at the single-cell level, facilitate a myriad ofanalyses. This study presents a novel method that seamlessly integrates CRISPR-Cas9 gene editingtechniques with single-cell isolation methods in induced pluripotent stem cell (hiPSC) lines, utilizingthe combined power of droplets and hydrogels. This innovative approach is designed to optimizeclonal selection, thereby concurrently reducing costs and the time required for generating a stablegenetically modified cell line. By bridging the advancements in gene editing and microfluidictechnologies, our approach not only holds significant promise for the development of disease modelsand assays but also addresses the crucial need for efficient single-cell isolation. This integrationcontributes to streamlining processes, making it a transformative method with implications forenhancing the efficiency and cost-effectiveness of stable cell line generation. As we navigate theintersection of gene editing and microfluidics, our study marks a significant stride toward innovativemethodologies in the dynamic landscape of cellular and molecular physiology research.Fil: Pérez Sosa, Camilo José. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perez, Maximiliano Sebastian. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vallejo Janeta, Alexander Paolo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; ArgentinaFil: Bhansali, Shekhar. Florida International University; Estados UnidosFil: Miriuka, Santiago Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia; ArgentinaFil: Lerner, Betiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; ArgentinaMDPI2024-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/238433Pérez Sosa, Camilo José; Perez, Maximiliano Sebastian; Vallejo Janeta, Alexander Paolo; Bhansali, Shekhar; Miriuka, Santiago Gabriel; et al.; Droplets for Gene Editing Using CRISPR-Cas9 and Clonal Selection Improvement Using Hydrogels; MDPI; Micromachines; 15; 3; 3-2024; 1-102072-666XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2072-666X/15/3/413info:eu-repo/semantics/altIdentifier/doi/10.3390/mi15030413info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:04Zoai:ri.conicet.gov.ar:11336/238433instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:04.654CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Droplets for Gene Editing Using CRISPR-Cas9 and Clonal Selection Improvement Using Hydrogels
title Droplets for Gene Editing Using CRISPR-Cas9 and Clonal Selection Improvement Using Hydrogels
spellingShingle Droplets for Gene Editing Using CRISPR-Cas9 and Clonal Selection Improvement Using Hydrogels
Pérez Sosa, Camilo José
MICROFLUIDICS
SINGLE CELL
DROPLETS
CRISPR-Cas9
title_short Droplets for Gene Editing Using CRISPR-Cas9 and Clonal Selection Improvement Using Hydrogels
title_full Droplets for Gene Editing Using CRISPR-Cas9 and Clonal Selection Improvement Using Hydrogels
title_fullStr Droplets for Gene Editing Using CRISPR-Cas9 and Clonal Selection Improvement Using Hydrogels
title_full_unstemmed Droplets for Gene Editing Using CRISPR-Cas9 and Clonal Selection Improvement Using Hydrogels
title_sort Droplets for Gene Editing Using CRISPR-Cas9 and Clonal Selection Improvement Using Hydrogels
dc.creator.none.fl_str_mv Pérez Sosa, Camilo José
Perez, Maximiliano Sebastian
Vallejo Janeta, Alexander Paolo
Bhansali, Shekhar
Miriuka, Santiago Gabriel
Lerner, Betiana
author Pérez Sosa, Camilo José
author_facet Pérez Sosa, Camilo José
Perez, Maximiliano Sebastian
Vallejo Janeta, Alexander Paolo
Bhansali, Shekhar
Miriuka, Santiago Gabriel
Lerner, Betiana
author_role author
author2 Perez, Maximiliano Sebastian
Vallejo Janeta, Alexander Paolo
Bhansali, Shekhar
Miriuka, Santiago Gabriel
Lerner, Betiana
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv MICROFLUIDICS
SINGLE CELL
DROPLETS
CRISPR-Cas9
topic MICROFLUIDICS
SINGLE CELL
DROPLETS
CRISPR-Cas9
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Gene editing tools have triggered a revolutionary transformation in the realms of cellularand molecular physiology, serving as a fundamental cornerstone for the evolution of disease modelsand assays in cell culture reactions, marked by various enhancements. Concurrently, microfluidicshas emerged over recent decades as a versatile technology capable of elevating performance andreducing costs in daily experiments across diverse scientific disciplines, with a pronounced impacton cell biology. The amalgamation of these groundbreaking techniques holds the potential to amplifythe generation of stable cell lines and the production of extracellular matrix hydrogels. Thesehydrogels, assuming a pivotal role in isolating cells at the single-cell level, facilitate a myriad ofanalyses. This study presents a novel method that seamlessly integrates CRISPR-Cas9 gene editingtechniques with single-cell isolation methods in induced pluripotent stem cell (hiPSC) lines, utilizingthe combined power of droplets and hydrogels. This innovative approach is designed to optimizeclonal selection, thereby concurrently reducing costs and the time required for generating a stablegenetically modified cell line. By bridging the advancements in gene editing and microfluidictechnologies, our approach not only holds significant promise for the development of disease modelsand assays but also addresses the crucial need for efficient single-cell isolation. This integrationcontributes to streamlining processes, making it a transformative method with implications forenhancing the efficiency and cost-effectiveness of stable cell line generation. As we navigate theintersection of gene editing and microfluidics, our study marks a significant stride toward innovativemethodologies in the dynamic landscape of cellular and molecular physiology research.
Fil: Pérez Sosa, Camilo José. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez, Maximiliano Sebastian. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vallejo Janeta, Alexander Paolo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina
Fil: Bhansali, Shekhar. Florida International University; Estados Unidos
Fil: Miriuka, Santiago Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia; Argentina
Fil: Lerner, Betiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnologica Nacional. Facultad Regional Haedo. Centro de Ingenieria de Recubrimientos Especiales y Nanoestructuras.; Argentina
description Gene editing tools have triggered a revolutionary transformation in the realms of cellularand molecular physiology, serving as a fundamental cornerstone for the evolution of disease modelsand assays in cell culture reactions, marked by various enhancements. Concurrently, microfluidicshas emerged over recent decades as a versatile technology capable of elevating performance andreducing costs in daily experiments across diverse scientific disciplines, with a pronounced impacton cell biology. The amalgamation of these groundbreaking techniques holds the potential to amplifythe generation of stable cell lines and the production of extracellular matrix hydrogels. Thesehydrogels, assuming a pivotal role in isolating cells at the single-cell level, facilitate a myriad ofanalyses. This study presents a novel method that seamlessly integrates CRISPR-Cas9 gene editingtechniques with single-cell isolation methods in induced pluripotent stem cell (hiPSC) lines, utilizingthe combined power of droplets and hydrogels. This innovative approach is designed to optimizeclonal selection, thereby concurrently reducing costs and the time required for generating a stablegenetically modified cell line. By bridging the advancements in gene editing and microfluidictechnologies, our approach not only holds significant promise for the development of disease modelsand assays but also addresses the crucial need for efficient single-cell isolation. This integrationcontributes to streamlining processes, making it a transformative method with implications forenhancing the efficiency and cost-effectiveness of stable cell line generation. As we navigate theintersection of gene editing and microfluidics, our study marks a significant stride toward innovativemethodologies in the dynamic landscape of cellular and molecular physiology research.
publishDate 2024
dc.date.none.fl_str_mv 2024-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/238433
Pérez Sosa, Camilo José; Perez, Maximiliano Sebastian; Vallejo Janeta, Alexander Paolo; Bhansali, Shekhar; Miriuka, Santiago Gabriel; et al.; Droplets for Gene Editing Using CRISPR-Cas9 and Clonal Selection Improvement Using Hydrogels; MDPI; Micromachines; 15; 3; 3-2024; 1-10
2072-666X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/238433
identifier_str_mv Pérez Sosa, Camilo José; Perez, Maximiliano Sebastian; Vallejo Janeta, Alexander Paolo; Bhansali, Shekhar; Miriuka, Santiago Gabriel; et al.; Droplets for Gene Editing Using CRISPR-Cas9 and Clonal Selection Improvement Using Hydrogels; MDPI; Micromachines; 15; 3; 3-2024; 1-10
2072-666X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2072-666X/15/3/413
info:eu-repo/semantics/altIdentifier/doi/10.3390/mi15030413
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269134033780736
score 13.13397

Publicaciones similares