NMR-based identification of metabolites in polar and non-polar extracts of avian liver

Autores
Fathi, Fariba; Brun, Antonio; Rott, Katherine H.; Cobra, Paulo Falco; Tonelli, Marco; Eghbalnia, Hamid R.; Caviedes Vidal, Enrique Juan Raul; Karasov, William; Markley, John L.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Metabolites present in liver provide important clues regarding the physiological state of an organism. The aim of this work was to evaluate a protocol for high-throughput NMR-based analysis of polar and non-polar metabolites from a small quantity of liver tissue. We extracted the tissue with a methanol/chloroform/water mixture and isolated the polar metabolites from the methanol/water layer and the non-polar metabolites from the chloroform layer. Following drying, we re-solubilized the fractions for analysis with a 600 MHz NMR spectrometer equipped with a 1.7 mm cryogenic probe. In order to evaluate the feasibility of this protocol for metabolomics studies, we analyzed the metabolic profile of livers from house sparrow (Passer domesticus) nestlings raised on two different diets: livers from 10 nestlings raised on a high protein diet (HP) for 4 d and livers from 12 nestlings raised on the HP diet for 3 d and then switched to a high carbohydrate diet (HC) for 1 d. The protocol enabled the detection of 52 polar and nine non-polar metabolites in 1H NMR spectra of the extracts. We analyzed the lipophilic metabolites by one-way ANOVA to assess statistically significant concentration differences between the two groups. The results of our studies demonstrate that the protocol described here can be exploited for high-throughput screening of small quantities of liver tissue (approx. 100 mg wet mass) obtainable from small animals.
Fil: Fathi, Fariba. University of Wisconsin; Estados Unidos
Fil: Brun, Antonio. University of Wisconsin; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rott, Katherine H.. University of Wisconsin; Estados Unidos
Fil: Cobra, Paulo Falco. University of Wisconsin; Estados Unidos
Fil: Tonelli, Marco. University of Wisconsin; Estados Unidos
Fil: Eghbalnia, Hamid R.. University of Wisconsin; Estados Unidos
Fil: Caviedes Vidal, Enrique Juan Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Karasov, William. University of Wisconsin; Estados Unidos
Fil: Markley, John L.. University of Wisconsin; Estados Unidos
Materia
DIET
EFFECT OF ON LIVER METABOLITES
EXTRACTION PROTOCOL
LIVER TISSUE
METABOLITE IDENTIFICATION BY NMR
NMR SPECTROSCOPY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67096
Acceder
id CONICETDig_540d7b0206a6ee1871d9149dfb86066b
oai_identifier_str oai:ri.conicet.gov.ar:11336/67096
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling NMR-based identification of metabolites in polar and non-polar extracts of avian liverFathi, FaribaBrun, AntonioRott, Katherine H.Cobra, Paulo FalcoTonelli, MarcoEghbalnia, Hamid R.Caviedes Vidal, Enrique Juan RaulKarasov, WilliamMarkley, John L.DIETEFFECT OF ON LIVER METABOLITESEXTRACTION PROTOCOLLIVER TISSUEMETABOLITE IDENTIFICATION BY NMRNMR SPECTROSCOPYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Metabolites present in liver provide important clues regarding the physiological state of an organism. The aim of this work was to evaluate a protocol for high-throughput NMR-based analysis of polar and non-polar metabolites from a small quantity of liver tissue. We extracted the tissue with a methanol/chloroform/water mixture and isolated the polar metabolites from the methanol/water layer and the non-polar metabolites from the chloroform layer. Following drying, we re-solubilized the fractions for analysis with a 600 MHz NMR spectrometer equipped with a 1.7 mm cryogenic probe. In order to evaluate the feasibility of this protocol for metabolomics studies, we analyzed the metabolic profile of livers from house sparrow (Passer domesticus) nestlings raised on two different diets: livers from 10 nestlings raised on a high protein diet (HP) for 4 d and livers from 12 nestlings raised on the HP diet for 3 d and then switched to a high carbohydrate diet (HC) for 1 d. The protocol enabled the detection of 52 polar and nine non-polar metabolites in 1H NMR spectra of the extracts. We analyzed the lipophilic metabolites by one-way ANOVA to assess statistically significant concentration differences between the two groups. The results of our studies demonstrate that the protocol described here can be exploited for high-throughput screening of small quantities of liver tissue (approx. 100 mg wet mass) obtainable from small animals.Fil: Fathi, Fariba. University of Wisconsin; Estados UnidosFil: Brun, Antonio. University of Wisconsin; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rott, Katherine H.. University of Wisconsin; Estados UnidosFil: Cobra, Paulo Falco. University of Wisconsin; Estados UnidosFil: Tonelli, Marco. University of Wisconsin; Estados UnidosFil: Eghbalnia, Hamid R.. University of Wisconsin; Estados UnidosFil: Caviedes Vidal, Enrique Juan Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Karasov, William. University of Wisconsin; Estados UnidosFil: Markley, John L.. University of Wisconsin; Estados UnidosMDPI2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67096Fathi, Fariba; Brun, Antonio; Rott, Katherine H.; Cobra, Paulo Falco; Tonelli, Marco; et al.; NMR-based identification of metabolites in polar and non-polar extracts of avian liver; MDPI; Metabolites; 7; 4; 12-2017; 1-92218-1989CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/metabo7040061info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2218-1989/7/4/61info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:09Zoai:ri.conicet.gov.ar:11336/67096instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:09.958CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv NMR-based identification of metabolites in polar and non-polar extracts of avian liver
title NMR-based identification of metabolites in polar and non-polar extracts of avian liver
spellingShingle NMR-based identification of metabolites in polar and non-polar extracts of avian liver
Fathi, Fariba
DIET
EFFECT OF ON LIVER METABOLITES
EXTRACTION PROTOCOL
LIVER TISSUE
METABOLITE IDENTIFICATION BY NMR
NMR SPECTROSCOPY
title_short NMR-based identification of metabolites in polar and non-polar extracts of avian liver
title_full NMR-based identification of metabolites in polar and non-polar extracts of avian liver
title_fullStr NMR-based identification of metabolites in polar and non-polar extracts of avian liver
title_full_unstemmed NMR-based identification of metabolites in polar and non-polar extracts of avian liver
title_sort NMR-based identification of metabolites in polar and non-polar extracts of avian liver
dc.creator.none.fl_str_mv Fathi, Fariba
Brun, Antonio
Rott, Katherine H.
Cobra, Paulo Falco
Tonelli, Marco
Eghbalnia, Hamid R.
Caviedes Vidal, Enrique Juan Raul
Karasov, William
Markley, John L.
author Fathi, Fariba
author_facet Fathi, Fariba
Brun, Antonio
Rott, Katherine H.
Cobra, Paulo Falco
Tonelli, Marco
Eghbalnia, Hamid R.
Caviedes Vidal, Enrique Juan Raul
Karasov, William
Markley, John L.
author_role author
author2 Brun, Antonio
Rott, Katherine H.
Cobra, Paulo Falco
Tonelli, Marco
Eghbalnia, Hamid R.
Caviedes Vidal, Enrique Juan Raul
Karasov, William
Markley, John L.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DIET
EFFECT OF ON LIVER METABOLITES
EXTRACTION PROTOCOL
LIVER TISSUE
METABOLITE IDENTIFICATION BY NMR
NMR SPECTROSCOPY
topic DIET
EFFECT OF ON LIVER METABOLITES
EXTRACTION PROTOCOL
LIVER TISSUE
METABOLITE IDENTIFICATION BY NMR
NMR SPECTROSCOPY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Metabolites present in liver provide important clues regarding the physiological state of an organism. The aim of this work was to evaluate a protocol for high-throughput NMR-based analysis of polar and non-polar metabolites from a small quantity of liver tissue. We extracted the tissue with a methanol/chloroform/water mixture and isolated the polar metabolites from the methanol/water layer and the non-polar metabolites from the chloroform layer. Following drying, we re-solubilized the fractions for analysis with a 600 MHz NMR spectrometer equipped with a 1.7 mm cryogenic probe. In order to evaluate the feasibility of this protocol for metabolomics studies, we analyzed the metabolic profile of livers from house sparrow (Passer domesticus) nestlings raised on two different diets: livers from 10 nestlings raised on a high protein diet (HP) for 4 d and livers from 12 nestlings raised on the HP diet for 3 d and then switched to a high carbohydrate diet (HC) for 1 d. The protocol enabled the detection of 52 polar and nine non-polar metabolites in 1H NMR spectra of the extracts. We analyzed the lipophilic metabolites by one-way ANOVA to assess statistically significant concentration differences between the two groups. The results of our studies demonstrate that the protocol described here can be exploited for high-throughput screening of small quantities of liver tissue (approx. 100 mg wet mass) obtainable from small animals.
Fil: Fathi, Fariba. University of Wisconsin; Estados Unidos
Fil: Brun, Antonio. University of Wisconsin; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rott, Katherine H.. University of Wisconsin; Estados Unidos
Fil: Cobra, Paulo Falco. University of Wisconsin; Estados Unidos
Fil: Tonelli, Marco. University of Wisconsin; Estados Unidos
Fil: Eghbalnia, Hamid R.. University of Wisconsin; Estados Unidos
Fil: Caviedes Vidal, Enrique Juan Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Karasov, William. University of Wisconsin; Estados Unidos
Fil: Markley, John L.. University of Wisconsin; Estados Unidos
description Metabolites present in liver provide important clues regarding the physiological state of an organism. The aim of this work was to evaluate a protocol for high-throughput NMR-based analysis of polar and non-polar metabolites from a small quantity of liver tissue. We extracted the tissue with a methanol/chloroform/water mixture and isolated the polar metabolites from the methanol/water layer and the non-polar metabolites from the chloroform layer. Following drying, we re-solubilized the fractions for analysis with a 600 MHz NMR spectrometer equipped with a 1.7 mm cryogenic probe. In order to evaluate the feasibility of this protocol for metabolomics studies, we analyzed the metabolic profile of livers from house sparrow (Passer domesticus) nestlings raised on two different diets: livers from 10 nestlings raised on a high protein diet (HP) for 4 d and livers from 12 nestlings raised on the HP diet for 3 d and then switched to a high carbohydrate diet (HC) for 1 d. The protocol enabled the detection of 52 polar and nine non-polar metabolites in 1H NMR spectra of the extracts. We analyzed the lipophilic metabolites by one-way ANOVA to assess statistically significant concentration differences between the two groups. The results of our studies demonstrate that the protocol described here can be exploited for high-throughput screening of small quantities of liver tissue (approx. 100 mg wet mass) obtainable from small animals.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67096
Fathi, Fariba; Brun, Antonio; Rott, Katherine H.; Cobra, Paulo Falco; Tonelli, Marco; et al.; NMR-based identification of metabolites in polar and non-polar extracts of avian liver; MDPI; Metabolites; 7; 4; 12-2017; 1-9
2218-1989
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67096
identifier_str_mv Fathi, Fariba; Brun, Antonio; Rott, Katherine H.; Cobra, Paulo Falco; Tonelli, Marco; et al.; NMR-based identification of metabolites in polar and non-polar extracts of avian liver; MDPI; Metabolites; 7; 4; 12-2017; 1-9
2218-1989
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/metabo7040061
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2218-1989/7/4/61
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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