Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supports

Autores
Vargas, Annie Y.; Rojas, Hugo A.; Romanelli, Gustavo Pablo; Martínez, José J.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of the urease immobilization method was studied on magnetic supports for the Biginelli/Hantzsch reaction. For this purpose, Fe3O4/SiO2 was modified with 3-Aminopropyl-Triethoxysilane and then activated with glutaraldehyde. A ratio of 500 mg of enzyme per gram of support at 4°C and 18 h were sufficient for the physical adsorption, while 24 h were required for covalent bonding. The Biginelli and Hantzsch reactions were used to evaluate urease application in multicomponent reactions (MCRs). The synthesis of 1,4-dihydropyrimidines was successfully performed using immobilized urease favoring the Hantzsch product. The magnetic properties of the supports allow easy separation, and the urease immobilized by both methods improved the enzymatic activity compared to that of free urease.
Fil: Vargas, Annie Y.. Universidad Pedagógica y Tecnológica de Colombia; Colombia
Fil: Rojas, Hugo A.. Universidad Pedagógica y Tecnológica de Colombia; Colombia
Fil: Romanelli, Gustavo Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas ; Argentina
Fil: Martínez, José J.. Universidad Pedagógica y Tecnológica de Colombia; Colombia
Materia
Biginelli/Hantzsch Reaction
Magnetic Supports
Urease Immobilized
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37970
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/37970
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supportsVargas, Annie Y.Rojas, Hugo A.Romanelli, Gustavo PabloMartínez, José J.Biginelli/Hantzsch ReactionMagnetic SupportsUrease Immobilizedhttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2The effect of the urease immobilization method was studied on magnetic supports for the Biginelli/Hantzsch reaction. For this purpose, Fe3O4/SiO2 was modified with 3-Aminopropyl-Triethoxysilane and then activated with glutaraldehyde. A ratio of 500 mg of enzyme per gram of support at 4°C and 18 h were sufficient for the physical adsorption, while 24 h were required for covalent bonding. The Biginelli and Hantzsch reactions were used to evaluate urease application in multicomponent reactions (MCRs). The synthesis of 1,4-dihydropyrimidines was successfully performed using immobilized urease favoring the Hantzsch product. The magnetic properties of the supports allow easy separation, and the urease immobilized by both methods improved the enzymatic activity compared to that of free urease.Fil: Vargas, Annie Y.. Universidad Pedagógica y Tecnológica de Colombia; ColombiaFil: Rojas, Hugo A.. Universidad Pedagógica y Tecnológica de Colombia; ColombiaFil: Romanelli, Gustavo Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas ; ArgentinaFil: Martínez, José J.. Universidad Pedagógica y Tecnológica de Colombia; ColombiaDe Gruyter2017-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37970Vargas, Annie Y.; Rojas, Hugo A.; Romanelli, Gustavo Pablo; Martínez, José J.; Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supports; De Gruyter; Green Processing and Synthesis; 6; 4; 8-2017; 377-3842191-9550CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1515/gps-2016-0143info:eu-repo/semantics/altIdentifier/url/https://www.degruyter.com/view/j/gps.2017.6.issue-4/gps-2016-0143/gps-2016-0143.xmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:17Zoai:ri.conicet.gov.ar:11336/37970instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:17.588CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supports
title Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supports
spellingShingle Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supports
Vargas, Annie Y.
Biginelli/Hantzsch Reaction
Magnetic Supports
Urease Immobilized
title_short Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supports
title_full Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supports
title_fullStr Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supports
title_full_unstemmed Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supports
title_sort Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supports
dc.creator.none.fl_str_mv Vargas, Annie Y.
Rojas, Hugo A.
Romanelli, Gustavo Pablo
Martínez, José J.
author Vargas, Annie Y.
author_facet Vargas, Annie Y.
Rojas, Hugo A.
Romanelli, Gustavo Pablo
Martínez, José J.
author_role author
author2 Rojas, Hugo A.
Romanelli, Gustavo Pablo
Martínez, José J.
author2_role author
author
author
dc.subject.none.fl_str_mv Biginelli/Hantzsch Reaction
Magnetic Supports
Urease Immobilized
topic Biginelli/Hantzsch Reaction
Magnetic Supports
Urease Immobilized
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The effect of the urease immobilization method was studied on magnetic supports for the Biginelli/Hantzsch reaction. For this purpose, Fe3O4/SiO2 was modified with 3-Aminopropyl-Triethoxysilane and then activated with glutaraldehyde. A ratio of 500 mg of enzyme per gram of support at 4°C and 18 h were sufficient for the physical adsorption, while 24 h were required for covalent bonding. The Biginelli and Hantzsch reactions were used to evaluate urease application in multicomponent reactions (MCRs). The synthesis of 1,4-dihydropyrimidines was successfully performed using immobilized urease favoring the Hantzsch product. The magnetic properties of the supports allow easy separation, and the urease immobilized by both methods improved the enzymatic activity compared to that of free urease.
Fil: Vargas, Annie Y.. Universidad Pedagógica y Tecnológica de Colombia; Colombia
Fil: Rojas, Hugo A.. Universidad Pedagógica y Tecnológica de Colombia; Colombia
Fil: Romanelli, Gustavo Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas ; Argentina
Fil: Martínez, José J.. Universidad Pedagógica y Tecnológica de Colombia; Colombia
description The effect of the urease immobilization method was studied on magnetic supports for the Biginelli/Hantzsch reaction. For this purpose, Fe3O4/SiO2 was modified with 3-Aminopropyl-Triethoxysilane and then activated with glutaraldehyde. A ratio of 500 mg of enzyme per gram of support at 4°C and 18 h were sufficient for the physical adsorption, while 24 h were required for covalent bonding. The Biginelli and Hantzsch reactions were used to evaluate urease application in multicomponent reactions (MCRs). The synthesis of 1,4-dihydropyrimidines was successfully performed using immobilized urease favoring the Hantzsch product. The magnetic properties of the supports allow easy separation, and the urease immobilized by both methods improved the enzymatic activity compared to that of free urease.
publishDate 2017
dc.date.none.fl_str_mv 2017-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37970
Vargas, Annie Y.; Rojas, Hugo A.; Romanelli, Gustavo Pablo; Martínez, José J.; Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supports; De Gruyter; Green Processing and Synthesis; 6; 4; 8-2017; 377-384
2191-9550
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37970
identifier_str_mv Vargas, Annie Y.; Rojas, Hugo A.; Romanelli, Gustavo Pablo; Martínez, José J.; Synthesis of 1,4-dihydropyrimidines with immobilized urease: Effect of method immobilization on magnetic supports; De Gruyter; Green Processing and Synthesis; 6; 4; 8-2017; 377-384
2191-9550
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1515/gps-2016-0143
info:eu-repo/semantics/altIdentifier/url/https://www.degruyter.com/view/j/gps.2017.6.issue-4/gps-2016-0143/gps-2016-0143.xml
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv De Gruyter
publisher.none.fl_str_mv De Gruyter
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268848677453824
score 13.13397

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