Neurotransmitter amines and antioxidant agents in neuronal protection against methylmercury-induced cytotoxicity in primary cultures of mice cortical neurons

Autores
Olguin, Nair Temis; Müller, Marie Lena; Rodríguez Farré, Eduard; Suñol, Cristina
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Methylmercury (MeHg) is an environmental toxicant with detrimental effects on the developing brain and adult nervous system. The main mechanisms identified include oxidative stress, changes in intracellular calcium, mitochondrial changes, inhibition of glutamate uptake, of protein synthesis and disruption of microtubules. However, little is known about mechanisms of protection against MeHg neurotoxicity. We found that resveratrol (10 μM) and ascorbic acid (200 μM) protected MeHg-induced cell death in primary cultures of cortical neurons. In this work, we aimed at finding additional targets that may be related to MeHg mode of action in cell toxicity with special emphasis in cell protection. We wonder whether neurotransmitters may affect the MeHg effects on neuronal death. Our findings show that neurons exposed to low MeHg concentrations exhibit less mortality if co-exposed to 10 μM dopamine (DA). However, DA metabolites, HVA (homovanillic acid) and DOPAC (3,4-dihydroxyphenylacetic acid) are not responsible for such protection. Furthermore, both DA D1 and D2 receptors agonists showed a protective effect against MeHg toxicity. It is striking though that DA receptor antagonists SKF83566 (10 μM) and haloperidol (10 μM) did not inhibit DA protection against MeHg. In addition, the protective effect of 10 μM DA against MeHg-induced toxicity was not affected by additional organochlorine pollutants exposure. Our results also demonstrate that cells exposed to MeHg in presence of 100 μM acetylcholine (ACh), show an increase in cell mortality at the “threshold value” of 100 nM MeHg. Finally, norepinephrine (10 μM) and serotonin (20 μM) also had an effect on cell protection. Altogether, we propose to further investigate the additional mechanisms that may be playing an important role in MeHg-induced cytotoxicity.
Fil: Olguin, Nair Temis. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Institut d’Investigacions Biomèdiques de Barcelona; España. Consejo Superior de Investigaciones Científicas; España
Fil: Müller, Marie Lena. Institut d’Investigacions Biomèdiques de Barcelona; España. Consejo Superior de Investigaciones Científicas; España
Fil: Rodríguez Farré, Eduard. Institut d’Investigacions Biomèdiques de Barcelona; España. Consejo Superior de Investigaciones Científicas; España
Fil: Suñol, Cristina. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; España
Materia
ANTIOXIDANTS
CELL VIABILITY
CULTURED CORTICAL NEURONS
DOPAMINE
METHYLMERCURY NEUROTOXICITY
NEUROPROTECTION
SEROTONIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/98238
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/98238
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Neurotransmitter amines and antioxidant agents in neuronal protection against methylmercury-induced cytotoxicity in primary cultures of mice cortical neuronsOlguin, Nair TemisMüller, Marie LenaRodríguez Farré, EduardSuñol, CristinaANTIOXIDANTSCELL VIABILITYCULTURED CORTICAL NEURONSDOPAMINEMETHYLMERCURY NEUROTOXICITYNEUROPROTECTIONSEROTONINhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Methylmercury (MeHg) is an environmental toxicant with detrimental effects on the developing brain and adult nervous system. The main mechanisms identified include oxidative stress, changes in intracellular calcium, mitochondrial changes, inhibition of glutamate uptake, of protein synthesis and disruption of microtubules. However, little is known about mechanisms of protection against MeHg neurotoxicity. We found that resveratrol (10 μM) and ascorbic acid (200 μM) protected MeHg-induced cell death in primary cultures of cortical neurons. In this work, we aimed at finding additional targets that may be related to MeHg mode of action in cell toxicity with special emphasis in cell protection. We wonder whether neurotransmitters may affect the MeHg effects on neuronal death. Our findings show that neurons exposed to low MeHg concentrations exhibit less mortality if co-exposed to 10 μM dopamine (DA). However, DA metabolites, HVA (homovanillic acid) and DOPAC (3,4-dihydroxyphenylacetic acid) are not responsible for such protection. Furthermore, both DA D1 and D2 receptors agonists showed a protective effect against MeHg toxicity. It is striking though that DA receptor antagonists SKF83566 (10 μM) and haloperidol (10 μM) did not inhibit DA protection against MeHg. In addition, the protective effect of 10 μM DA against MeHg-induced toxicity was not affected by additional organochlorine pollutants exposure. Our results also demonstrate that cells exposed to MeHg in presence of 100 μM acetylcholine (ACh), show an increase in cell mortality at the “threshold value” of 100 nM MeHg. Finally, norepinephrine (10 μM) and serotonin (20 μM) also had an effect on cell protection. Altogether, we propose to further investigate the additional mechanisms that may be playing an important role in MeHg-induced cytotoxicity.Fil: Olguin, Nair Temis. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Institut d’Investigacions Biomèdiques de Barcelona; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Müller, Marie Lena. Institut d’Investigacions Biomèdiques de Barcelona; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Rodríguez Farré, Eduard. Institut d’Investigacions Biomèdiques de Barcelona; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Suñol, Cristina. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; EspañaElsevier Science2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/98238Olguin, Nair Temis; Müller, Marie Lena; Rodríguez Farré, Eduard; Suñol, Cristina; Neurotransmitter amines and antioxidant agents in neuronal protection against methylmercury-induced cytotoxicity in primary cultures of mice cortical neurons; Elsevier Science; Neurotoxicology; 69; 12-2018; 278-2870161-813XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuro.2018.07.020info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0161813X18303097info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:07:16Zoai:ri.conicet.gov.ar:11336/98238instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:07:16.567CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neurotransmitter amines and antioxidant agents in neuronal protection against methylmercury-induced cytotoxicity in primary cultures of mice cortical neurons
title Neurotransmitter amines and antioxidant agents in neuronal protection against methylmercury-induced cytotoxicity in primary cultures of mice cortical neurons
spellingShingle Neurotransmitter amines and antioxidant agents in neuronal protection against methylmercury-induced cytotoxicity in primary cultures of mice cortical neurons
Olguin, Nair Temis
ANTIOXIDANTS
CELL VIABILITY
CULTURED CORTICAL NEURONS
DOPAMINE
METHYLMERCURY NEUROTOXICITY
NEUROPROTECTION
SEROTONIN
title_short Neurotransmitter amines and antioxidant agents in neuronal protection against methylmercury-induced cytotoxicity in primary cultures of mice cortical neurons
title_full Neurotransmitter amines and antioxidant agents in neuronal protection against methylmercury-induced cytotoxicity in primary cultures of mice cortical neurons
title_fullStr Neurotransmitter amines and antioxidant agents in neuronal protection against methylmercury-induced cytotoxicity in primary cultures of mice cortical neurons
title_full_unstemmed Neurotransmitter amines and antioxidant agents in neuronal protection against methylmercury-induced cytotoxicity in primary cultures of mice cortical neurons
title_sort Neurotransmitter amines and antioxidant agents in neuronal protection against methylmercury-induced cytotoxicity in primary cultures of mice cortical neurons
dc.creator.none.fl_str_mv Olguin, Nair Temis
Müller, Marie Lena
Rodríguez Farré, Eduard
Suñol, Cristina
author Olguin, Nair Temis
author_facet Olguin, Nair Temis
Müller, Marie Lena
Rodríguez Farré, Eduard
Suñol, Cristina
author_role author
author2 Müller, Marie Lena
Rodríguez Farré, Eduard
Suñol, Cristina
author2_role author
author
author
dc.subject.none.fl_str_mv ANTIOXIDANTS
CELL VIABILITY
CULTURED CORTICAL NEURONS
DOPAMINE
METHYLMERCURY NEUROTOXICITY
NEUROPROTECTION
SEROTONIN
topic ANTIOXIDANTS
CELL VIABILITY
CULTURED CORTICAL NEURONS
DOPAMINE
METHYLMERCURY NEUROTOXICITY
NEUROPROTECTION
SEROTONIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Methylmercury (MeHg) is an environmental toxicant with detrimental effects on the developing brain and adult nervous system. The main mechanisms identified include oxidative stress, changes in intracellular calcium, mitochondrial changes, inhibition of glutamate uptake, of protein synthesis and disruption of microtubules. However, little is known about mechanisms of protection against MeHg neurotoxicity. We found that resveratrol (10 μM) and ascorbic acid (200 μM) protected MeHg-induced cell death in primary cultures of cortical neurons. In this work, we aimed at finding additional targets that may be related to MeHg mode of action in cell toxicity with special emphasis in cell protection. We wonder whether neurotransmitters may affect the MeHg effects on neuronal death. Our findings show that neurons exposed to low MeHg concentrations exhibit less mortality if co-exposed to 10 μM dopamine (DA). However, DA metabolites, HVA (homovanillic acid) and DOPAC (3,4-dihydroxyphenylacetic acid) are not responsible for such protection. Furthermore, both DA D1 and D2 receptors agonists showed a protective effect against MeHg toxicity. It is striking though that DA receptor antagonists SKF83566 (10 μM) and haloperidol (10 μM) did not inhibit DA protection against MeHg. In addition, the protective effect of 10 μM DA against MeHg-induced toxicity was not affected by additional organochlorine pollutants exposure. Our results also demonstrate that cells exposed to MeHg in presence of 100 μM acetylcholine (ACh), show an increase in cell mortality at the “threshold value” of 100 nM MeHg. Finally, norepinephrine (10 μM) and serotonin (20 μM) also had an effect on cell protection. Altogether, we propose to further investigate the additional mechanisms that may be playing an important role in MeHg-induced cytotoxicity.
Fil: Olguin, Nair Temis. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Institut d’Investigacions Biomèdiques de Barcelona; España. Consejo Superior de Investigaciones Científicas; España
Fil: Müller, Marie Lena. Institut d’Investigacions Biomèdiques de Barcelona; España. Consejo Superior de Investigaciones Científicas; España
Fil: Rodríguez Farré, Eduard. Institut d’Investigacions Biomèdiques de Barcelona; España. Consejo Superior de Investigaciones Científicas; España
Fil: Suñol, Cristina. Consejo Superior de Investigaciones Científicas; España. Institut d’Investigacions Biomèdiques de Barcelona; España
description Methylmercury (MeHg) is an environmental toxicant with detrimental effects on the developing brain and adult nervous system. The main mechanisms identified include oxidative stress, changes in intracellular calcium, mitochondrial changes, inhibition of glutamate uptake, of protein synthesis and disruption of microtubules. However, little is known about mechanisms of protection against MeHg neurotoxicity. We found that resveratrol (10 μM) and ascorbic acid (200 μM) protected MeHg-induced cell death in primary cultures of cortical neurons. In this work, we aimed at finding additional targets that may be related to MeHg mode of action in cell toxicity with special emphasis in cell protection. We wonder whether neurotransmitters may affect the MeHg effects on neuronal death. Our findings show that neurons exposed to low MeHg concentrations exhibit less mortality if co-exposed to 10 μM dopamine (DA). However, DA metabolites, HVA (homovanillic acid) and DOPAC (3,4-dihydroxyphenylacetic acid) are not responsible for such protection. Furthermore, both DA D1 and D2 receptors agonists showed a protective effect against MeHg toxicity. It is striking though that DA receptor antagonists SKF83566 (10 μM) and haloperidol (10 μM) did not inhibit DA protection against MeHg. In addition, the protective effect of 10 μM DA against MeHg-induced toxicity was not affected by additional organochlorine pollutants exposure. Our results also demonstrate that cells exposed to MeHg in presence of 100 μM acetylcholine (ACh), show an increase in cell mortality at the “threshold value” of 100 nM MeHg. Finally, norepinephrine (10 μM) and serotonin (20 μM) also had an effect on cell protection. Altogether, we propose to further investigate the additional mechanisms that may be playing an important role in MeHg-induced cytotoxicity.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/98238
Olguin, Nair Temis; Müller, Marie Lena; Rodríguez Farré, Eduard; Suñol, Cristina; Neurotransmitter amines and antioxidant agents in neuronal protection against methylmercury-induced cytotoxicity in primary cultures of mice cortical neurons; Elsevier Science; Neurotoxicology; 69; 12-2018; 278-287
0161-813X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/98238
identifier_str_mv Olguin, Nair Temis; Müller, Marie Lena; Rodríguez Farré, Eduard; Suñol, Cristina; Neurotransmitter amines and antioxidant agents in neuronal protection against methylmercury-induced cytotoxicity in primary cultures of mice cortical neurons; Elsevier Science; Neurotoxicology; 69; 12-2018; 278-287
0161-813X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuro.2018.07.020
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0161813X18303097
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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