Microenvironments to study migration and somal translocation in cortical neurons

Autores
Zhao, Shifang; Fan, Wenqiang; Guo, Xiang; Xue, Longjian; Berninger, Benedikt; Salierno, Marcelo Javier; del Campo, Aránzazu
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Migrating post-mitotic neurons of the developing cerebral cortex undergo terminal somal translocation (ST) when they reach their final destination in the cortical plate. This process is crucial for proper cortical layering and its perturbation can lead to brain dysfunction. Here we present a reductionist biomaterials platform that faithfully supports and controls the distinct phases of terminal ST in vitro. We developed microenvironments with different adhesive molecules to support neuronal attachment, neurite extension, and migration in distinct manners. Efficient ST occurred when the leading process of migratory neurons crossed from low-to high-adhesive areas on a substrate, promoting spreading of the leading growth cone. Our results indicate that elementary adhesive cell-substrate interactions strongly influence migratory behavior and the final positioning of neurons during their developmental journey. This in vitro model allows advanced experimentation to reveal the microenvironmental requirements underlying cortical layer development and disorders.
Fil: Zhao, Shifang. Leibniz Institute for New Materials; Alemania. Max Planck Institute für Polymerforschung; Alemania. Universitat Saarland; Alemania
Fil: Fan, Wenqiang. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Guo, Xiang. Leibniz Institute for New Materials; Alemania
Fil: Xue, Longjian. Max Planck Institute für Polymerforschung; Alemania
Fil: Berninger, Benedikt. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Salierno, Marcelo Javier. Max Planck Institute für Polymerforschung; Alemania. Johannes Gutenberg Universitat Mainz; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
Fil: del Campo, Aránzazu. Max Planck Institute für Polymerforschung; Alemania. Universitat Saarland; Alemania. Leibniz Institute for New Materials; Alemania
Materia
CEREBRAL CORTEX
CORTICAL NEURONS
CORTICOGENESIS
NEURONAL MIGRATION
SOMAL TRANSLOCATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93323
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Microenvironments to study migration and somal translocation in cortical neuronsZhao, ShifangFan, WenqiangGuo, XiangXue, LongjianBerninger, BenediktSalierno, Marcelo Javierdel Campo, AránzazuCEREBRAL CORTEXCORTICAL NEURONSCORTICOGENESISNEURONAL MIGRATIONSOMAL TRANSLOCATIONhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Migrating post-mitotic neurons of the developing cerebral cortex undergo terminal somal translocation (ST) when they reach their final destination in the cortical plate. This process is crucial for proper cortical layering and its perturbation can lead to brain dysfunction. Here we present a reductionist biomaterials platform that faithfully supports and controls the distinct phases of terminal ST in vitro. We developed microenvironments with different adhesive molecules to support neuronal attachment, neurite extension, and migration in distinct manners. Efficient ST occurred when the leading process of migratory neurons crossed from low-to high-adhesive areas on a substrate, promoting spreading of the leading growth cone. Our results indicate that elementary adhesive cell-substrate interactions strongly influence migratory behavior and the final positioning of neurons during their developmental journey. This in vitro model allows advanced experimentation to reveal the microenvironmental requirements underlying cortical layer development and disorders.Fil: Zhao, Shifang. Leibniz Institute for New Materials; Alemania. Max Planck Institute für Polymerforschung; Alemania. Universitat Saarland; AlemaniaFil: Fan, Wenqiang. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Guo, Xiang. Leibniz Institute for New Materials; AlemaniaFil: Xue, Longjian. Max Planck Institute für Polymerforschung; AlemaniaFil: Berninger, Benedikt. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Salierno, Marcelo Javier. Max Planck Institute für Polymerforschung; Alemania. Johannes Gutenberg Universitat Mainz; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; ArgentinaFil: del Campo, Aránzazu. Max Planck Institute für Polymerforschung; Alemania. Universitat Saarland; Alemania. Leibniz Institute for New Materials; AlemaniaElsevier2018-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93323Zhao, Shifang; Fan, Wenqiang; Guo, Xiang; Xue, Longjian; Berninger, Benedikt; et al.; Microenvironments to study migration and somal translocation in cortical neurons; Elsevier; Biomaterials; 156; 2-2018; 238-2470142-9612CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.biomaterials.2017.11.042info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0142961217307743info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:28Zoai:ri.conicet.gov.ar:11336/93323instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:28.652CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Microenvironments to study migration and somal translocation in cortical neurons
title Microenvironments to study migration and somal translocation in cortical neurons
spellingShingle Microenvironments to study migration and somal translocation in cortical neurons
Zhao, Shifang
CEREBRAL CORTEX
CORTICAL NEURONS
CORTICOGENESIS
NEURONAL MIGRATION
SOMAL TRANSLOCATION
title_short Microenvironments to study migration and somal translocation in cortical neurons
title_full Microenvironments to study migration and somal translocation in cortical neurons
title_fullStr Microenvironments to study migration and somal translocation in cortical neurons
title_full_unstemmed Microenvironments to study migration and somal translocation in cortical neurons
title_sort Microenvironments to study migration and somal translocation in cortical neurons
dc.creator.none.fl_str_mv Zhao, Shifang
Fan, Wenqiang
Guo, Xiang
Xue, Longjian
Berninger, Benedikt
Salierno, Marcelo Javier
del Campo, Aránzazu
author Zhao, Shifang
author_facet Zhao, Shifang
Fan, Wenqiang
Guo, Xiang
Xue, Longjian
Berninger, Benedikt
Salierno, Marcelo Javier
del Campo, Aránzazu
author_role author
author2 Fan, Wenqiang
Guo, Xiang
Xue, Longjian
Berninger, Benedikt
Salierno, Marcelo Javier
del Campo, Aránzazu
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CEREBRAL CORTEX
CORTICAL NEURONS
CORTICOGENESIS
NEURONAL MIGRATION
SOMAL TRANSLOCATION
topic CEREBRAL CORTEX
CORTICAL NEURONS
CORTICOGENESIS
NEURONAL MIGRATION
SOMAL TRANSLOCATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Migrating post-mitotic neurons of the developing cerebral cortex undergo terminal somal translocation (ST) when they reach their final destination in the cortical plate. This process is crucial for proper cortical layering and its perturbation can lead to brain dysfunction. Here we present a reductionist biomaterials platform that faithfully supports and controls the distinct phases of terminal ST in vitro. We developed microenvironments with different adhesive molecules to support neuronal attachment, neurite extension, and migration in distinct manners. Efficient ST occurred when the leading process of migratory neurons crossed from low-to high-adhesive areas on a substrate, promoting spreading of the leading growth cone. Our results indicate that elementary adhesive cell-substrate interactions strongly influence migratory behavior and the final positioning of neurons during their developmental journey. This in vitro model allows advanced experimentation to reveal the microenvironmental requirements underlying cortical layer development and disorders.
Fil: Zhao, Shifang. Leibniz Institute for New Materials; Alemania. Max Planck Institute für Polymerforschung; Alemania. Universitat Saarland; Alemania
Fil: Fan, Wenqiang. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Guo, Xiang. Leibniz Institute for New Materials; Alemania
Fil: Xue, Longjian. Max Planck Institute für Polymerforschung; Alemania
Fil: Berninger, Benedikt. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Salierno, Marcelo Javier. Max Planck Institute für Polymerforschung; Alemania. Johannes Gutenberg Universitat Mainz; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
Fil: del Campo, Aránzazu. Max Planck Institute für Polymerforschung; Alemania. Universitat Saarland; Alemania. Leibniz Institute for New Materials; Alemania
description Migrating post-mitotic neurons of the developing cerebral cortex undergo terminal somal translocation (ST) when they reach their final destination in the cortical plate. This process is crucial for proper cortical layering and its perturbation can lead to brain dysfunction. Here we present a reductionist biomaterials platform that faithfully supports and controls the distinct phases of terminal ST in vitro. We developed microenvironments with different adhesive molecules to support neuronal attachment, neurite extension, and migration in distinct manners. Efficient ST occurred when the leading process of migratory neurons crossed from low-to high-adhesive areas on a substrate, promoting spreading of the leading growth cone. Our results indicate that elementary adhesive cell-substrate interactions strongly influence migratory behavior and the final positioning of neurons during their developmental journey. This in vitro model allows advanced experimentation to reveal the microenvironmental requirements underlying cortical layer development and disorders.
publishDate 2018
dc.date.none.fl_str_mv 2018-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93323
Zhao, Shifang; Fan, Wenqiang; Guo, Xiang; Xue, Longjian; Berninger, Benedikt; et al.; Microenvironments to study migration and somal translocation in cortical neurons; Elsevier; Biomaterials; 156; 2-2018; 238-247
0142-9612
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93323
identifier_str_mv Zhao, Shifang; Fan, Wenqiang; Guo, Xiang; Xue, Longjian; Berninger, Benedikt; et al.; Microenvironments to study migration and somal translocation in cortical neurons; Elsevier; Biomaterials; 156; 2-2018; 238-247
0142-9612
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biomaterials.2017.11.042
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0142961217307743
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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