Chlamydia trachomatis infection leads to defined alterations to the lipid droplet proteome in epithelial cells

Autores
Saka, Hector Alex; Thompson, J. Will; Chen, Yi-Shan; Dubois, Laura G.; Haas, Joel T.; Moseley, Arthur; Valdivia, Raphael H.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The obligate intracellular bacterium Chlamydia trachomatisis a major human pathogen and a main cause of genital and ocular diseases. During its intracellular cycle, C. trachomatisreplicates inside a membrane-bound vacuole termed an "inclusion". Acquisition of lipids (and other nutrients) from the host cell is a critical step in chlamydial replication. Lipid droplets (LD) are ubiquitous, ER-derived neutral lipid-rich storage organelles surrounded by a phospholipids monolayer and associated proteins. Previous studies have shown that LDs accumulate at the periphery of, and eventually translocate into, the chlamydial inclusion. These observations point out to Chlamydia-mediated manipulation of LDs in infected cells, which may impact the function and thereby the protein composition of these organelles. By means of a label-free quantitative mass spectrometry approach we found that the LD proteome is modified in the context of C. trachomatis infection. We determined that LDs isolated from C. trachomatis- infected cells were enriched in proteins related to lipid metabolism, biosynthesis and LD-specific functions. Interestingly, consistent with the observation that LDs intimately associate with the inclusion, a subset of inclusion membrane proteins co-purified with LD protein extracts. Finally, genetic ablation of LDs negatively affected generation of C. trachomatis infectious progeny, consistent with a role for LD biogenesis in optimal chlamydial growth.
Fil: Saka, Hector Alex. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. University of Duke; Estados Unidos
Fil: Thompson, J. Will. Duke University Medical Center; Estados Unidos
Fil: Chen, Yi-Shan. Duke University Medical Center; Estados Unidos
Fil: Dubois, Laura G.. Duke University Medical Center; Estados Unidos
Fil: Haas, Joel T.. Dept. Of Biochemistry And Biophysics, Ucsf; Estados Unidos
Fil: Moseley, Arthur. Duke University Medical Center; Estados Unidos
Fil: Valdivia, Raphael H.. Duke University Medical Center; Estados Unidos
Materia
CHLAMYDIA TRACHOMATIS
LIPID DROPLETS
HOST PATHOGEN INTERACTIONS
QUANTITATIVE PROTEOMICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/59274
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/59274
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chlamydia trachomatis infection leads to defined alterations to the lipid droplet proteome in epithelial cellsSaka, Hector AlexThompson, J. WillChen, Yi-ShanDubois, Laura G.Haas, Joel T.Moseley, ArthurValdivia, Raphael H.CHLAMYDIA TRACHOMATISLIPID DROPLETSHOST PATHOGEN INTERACTIONSQUANTITATIVE PROTEOMICShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The obligate intracellular bacterium Chlamydia trachomatisis a major human pathogen and a main cause of genital and ocular diseases. During its intracellular cycle, C. trachomatisreplicates inside a membrane-bound vacuole termed an "inclusion". Acquisition of lipids (and other nutrients) from the host cell is a critical step in chlamydial replication. Lipid droplets (LD) are ubiquitous, ER-derived neutral lipid-rich storage organelles surrounded by a phospholipids monolayer and associated proteins. Previous studies have shown that LDs accumulate at the periphery of, and eventually translocate into, the chlamydial inclusion. These observations point out to Chlamydia-mediated manipulation of LDs in infected cells, which may impact the function and thereby the protein composition of these organelles. By means of a label-free quantitative mass spectrometry approach we found that the LD proteome is modified in the context of C. trachomatis infection. We determined that LDs isolated from C. trachomatis- infected cells were enriched in proteins related to lipid metabolism, biosynthesis and LD-specific functions. Interestingly, consistent with the observation that LDs intimately associate with the inclusion, a subset of inclusion membrane proteins co-purified with LD protein extracts. Finally, genetic ablation of LDs negatively affected generation of C. trachomatis infectious progeny, consistent with a role for LD biogenesis in optimal chlamydial growth.Fil: Saka, Hector Alex. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. University of Duke; Estados UnidosFil: Thompson, J. Will. Duke University Medical Center; Estados UnidosFil: Chen, Yi-Shan. Duke University Medical Center; Estados UnidosFil: Dubois, Laura G.. Duke University Medical Center; Estados UnidosFil: Haas, Joel T.. Dept. Of Biochemistry And Biophysics, Ucsf; Estados UnidosFil: Moseley, Arthur. Duke University Medical Center; Estados UnidosFil: Valdivia, Raphael H.. Duke University Medical Center; Estados UnidosPublic Library of Science2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/59274Saka, Hector Alex; Thompson, J. Will; Chen, Yi-Shan; Dubois, Laura G.; Haas, Joel T.; et al.; Chlamydia trachomatis infection leads to defined alterations to the lipid droplet proteome in epithelial cells; Public Library of Science; Plos One; 10; 4; 4-20151932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/25909443info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0124630info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:58Zoai:ri.conicet.gov.ar:11336/59274instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:59.201CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chlamydia trachomatis infection leads to defined alterations to the lipid droplet proteome in epithelial cells
title Chlamydia trachomatis infection leads to defined alterations to the lipid droplet proteome in epithelial cells
spellingShingle Chlamydia trachomatis infection leads to defined alterations to the lipid droplet proteome in epithelial cells
Saka, Hector Alex
CHLAMYDIA TRACHOMATIS
LIPID DROPLETS
HOST PATHOGEN INTERACTIONS
QUANTITATIVE PROTEOMICS
title_short Chlamydia trachomatis infection leads to defined alterations to the lipid droplet proteome in epithelial cells
title_full Chlamydia trachomatis infection leads to defined alterations to the lipid droplet proteome in epithelial cells
title_fullStr Chlamydia trachomatis infection leads to defined alterations to the lipid droplet proteome in epithelial cells
title_full_unstemmed Chlamydia trachomatis infection leads to defined alterations to the lipid droplet proteome in epithelial cells
title_sort Chlamydia trachomatis infection leads to defined alterations to the lipid droplet proteome in epithelial cells
dc.creator.none.fl_str_mv Saka, Hector Alex
Thompson, J. Will
Chen, Yi-Shan
Dubois, Laura G.
Haas, Joel T.
Moseley, Arthur
Valdivia, Raphael H.
author Saka, Hector Alex
author_facet Saka, Hector Alex
Thompson, J. Will
Chen, Yi-Shan
Dubois, Laura G.
Haas, Joel T.
Moseley, Arthur
Valdivia, Raphael H.
author_role author
author2 Thompson, J. Will
Chen, Yi-Shan
Dubois, Laura G.
Haas, Joel T.
Moseley, Arthur
Valdivia, Raphael H.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CHLAMYDIA TRACHOMATIS
LIPID DROPLETS
HOST PATHOGEN INTERACTIONS
QUANTITATIVE PROTEOMICS
topic CHLAMYDIA TRACHOMATIS
LIPID DROPLETS
HOST PATHOGEN INTERACTIONS
QUANTITATIVE PROTEOMICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The obligate intracellular bacterium Chlamydia trachomatisis a major human pathogen and a main cause of genital and ocular diseases. During its intracellular cycle, C. trachomatisreplicates inside a membrane-bound vacuole termed an "inclusion". Acquisition of lipids (and other nutrients) from the host cell is a critical step in chlamydial replication. Lipid droplets (LD) are ubiquitous, ER-derived neutral lipid-rich storage organelles surrounded by a phospholipids monolayer and associated proteins. Previous studies have shown that LDs accumulate at the periphery of, and eventually translocate into, the chlamydial inclusion. These observations point out to Chlamydia-mediated manipulation of LDs in infected cells, which may impact the function and thereby the protein composition of these organelles. By means of a label-free quantitative mass spectrometry approach we found that the LD proteome is modified in the context of C. trachomatis infection. We determined that LDs isolated from C. trachomatis- infected cells were enriched in proteins related to lipid metabolism, biosynthesis and LD-specific functions. Interestingly, consistent with the observation that LDs intimately associate with the inclusion, a subset of inclusion membrane proteins co-purified with LD protein extracts. Finally, genetic ablation of LDs negatively affected generation of C. trachomatis infectious progeny, consistent with a role for LD biogenesis in optimal chlamydial growth.
Fil: Saka, Hector Alex. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. University of Duke; Estados Unidos
Fil: Thompson, J. Will. Duke University Medical Center; Estados Unidos
Fil: Chen, Yi-Shan. Duke University Medical Center; Estados Unidos
Fil: Dubois, Laura G.. Duke University Medical Center; Estados Unidos
Fil: Haas, Joel T.. Dept. Of Biochemistry And Biophysics, Ucsf; Estados Unidos
Fil: Moseley, Arthur. Duke University Medical Center; Estados Unidos
Fil: Valdivia, Raphael H.. Duke University Medical Center; Estados Unidos
description The obligate intracellular bacterium Chlamydia trachomatisis a major human pathogen and a main cause of genital and ocular diseases. During its intracellular cycle, C. trachomatisreplicates inside a membrane-bound vacuole termed an "inclusion". Acquisition of lipids (and other nutrients) from the host cell is a critical step in chlamydial replication. Lipid droplets (LD) are ubiquitous, ER-derived neutral lipid-rich storage organelles surrounded by a phospholipids monolayer and associated proteins. Previous studies have shown that LDs accumulate at the periphery of, and eventually translocate into, the chlamydial inclusion. These observations point out to Chlamydia-mediated manipulation of LDs in infected cells, which may impact the function and thereby the protein composition of these organelles. By means of a label-free quantitative mass spectrometry approach we found that the LD proteome is modified in the context of C. trachomatis infection. We determined that LDs isolated from C. trachomatis- infected cells were enriched in proteins related to lipid metabolism, biosynthesis and LD-specific functions. Interestingly, consistent with the observation that LDs intimately associate with the inclusion, a subset of inclusion membrane proteins co-purified with LD protein extracts. Finally, genetic ablation of LDs negatively affected generation of C. trachomatis infectious progeny, consistent with a role for LD biogenesis in optimal chlamydial growth.
publishDate 2015
dc.date.none.fl_str_mv 2015-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/59274
Saka, Hector Alex; Thompson, J. Will; Chen, Yi-Shan; Dubois, Laura G.; Haas, Joel T.; et al.; Chlamydia trachomatis infection leads to defined alterations to the lipid droplet proteome in epithelial cells; Public Library of Science; Plos One; 10; 4; 4-2015
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/59274
identifier_str_mv Saka, Hector Alex; Thompson, J. Will; Chen, Yi-Shan; Dubois, Laura G.; Haas, Joel T.; et al.; Chlamydia trachomatis infection leads to defined alterations to the lipid droplet proteome in epithelial cells; Public Library of Science; Plos One; 10; 4; 4-2015
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/25909443
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0124630
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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