Trypanosoma cruzi: Infectivity modulation of a clone after passages through different hosts

Autores
Pérez Brandan, Cecilia María; Padilla, Ángel Marcelo; Diosque, Patricio; Basombrío, Miguel Ángel Manuel
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although Trypanosoma cruzi virulence can be modified through passages in vivo or long-term in vitro culture, the mechanisms involved are poorly understood. Here we report modifications in the infectivity of a T. cruzi clone after passages in different hosts without detectable changes in parasite genetic patterns. A clone was obtained from a T. cruzi IIe isolate and showed to be less virulent than the original isolate (p < 0.05). This clone was enzymatically similar to the original isolate as shown by multilocus enzyme electrophoresis. Infection of this clone was compared by successive passages in mice and guinea pigs. The mouse-passaged subline became more virulent for both host species compared to the guinea pig-passaged subline (p < 0.05). The clone line displayed similar random amplified polymorphic DNA patterns before and after passages in different hosts suggesting that alterations in virulence could be a result of a differential expression of virulence factors.
Fil: Pérez Brandan, Cecilia María. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Padilla, Ángel Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina
Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina
Fil: Basombrío, Miguel Ángel Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina
Materia
Clone Line from Tolac1 Isolate
Clones
Cta1
Deoxyribonucleic Acid
Dna
Glucose-6-Phosphate Isomerase
Gpi
Infectivity
Lit
Lit Plus Fetal Bovine Serum, Hemin, And Penicillin-Streptomycin
Lit-Hsp
Liver Infusion Tryptose Medium
Mlee
Multilocus Enzyme Electrophoresis
Random Amplified Polymorphic Dna
Rapd
Ribonucleic Acid
Rna
Selection
Tae
Tris-Acetate Edta
Trypanosoma Cruzi
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36596

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oai_identifier_str oai:ri.conicet.gov.ar:11336/36596
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Trypanosoma cruzi: Infectivity modulation of a clone after passages through different hostsPérez Brandan, Cecilia MaríaPadilla, Ángel MarceloDiosque, PatricioBasombrío, Miguel Ángel ManuelClone Line from Tolac1 IsolateClonesCta1Deoxyribonucleic AcidDnaGlucose-6-Phosphate IsomeraseGpiInfectivityLitLit Plus Fetal Bovine Serum, Hemin, And Penicillin-StreptomycinLit-HspLiver Infusion Tryptose MediumMleeMultilocus Enzyme ElectrophoresisRandom Amplified Polymorphic DnaRapdRibonucleic AcidRnaSelectionTaeTris-Acetate EdtaTrypanosoma Cruzihttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Although Trypanosoma cruzi virulence can be modified through passages in vivo or long-term in vitro culture, the mechanisms involved are poorly understood. Here we report modifications in the infectivity of a T. cruzi clone after passages in different hosts without detectable changes in parasite genetic patterns. A clone was obtained from a T. cruzi IIe isolate and showed to be less virulent than the original isolate (p < 0.05). This clone was enzymatically similar to the original isolate as shown by multilocus enzyme electrophoresis. Infection of this clone was compared by successive passages in mice and guinea pigs. The mouse-passaged subline became more virulent for both host species compared to the guinea pig-passaged subline (p < 0.05). The clone line displayed similar random amplified polymorphic DNA patterns before and after passages in different hosts suggesting that alterations in virulence could be a result of a differential expression of virulence factors.Fil: Pérez Brandan, Cecilia María. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Padilla, Ángel Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; ArgentinaFil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; ArgentinaFil: Basombrío, Miguel Ángel Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; ArgentinaElsevier2006-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36596Pérez Brandan, Cecilia María; Padilla, Ángel Marcelo; Diosque, Patricio; Basombrío, Miguel Ángel Manuel; Trypanosoma cruzi: Infectivity modulation of a clone after passages through different hosts; Elsevier; Experimental Parasitology; 114; 2; 10-2006; 89-930014-4894CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.exppara.2006.02.014info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014489406000439info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:20:34Zoai:ri.conicet.gov.ar:11336/36596instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:20:35.188CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Trypanosoma cruzi: Infectivity modulation of a clone after passages through different hosts
title Trypanosoma cruzi: Infectivity modulation of a clone after passages through different hosts
spellingShingle Trypanosoma cruzi: Infectivity modulation of a clone after passages through different hosts
Pérez Brandan, Cecilia María
Clone Line from Tolac1 Isolate
Clones
Cta1
Deoxyribonucleic Acid
Dna
Glucose-6-Phosphate Isomerase
Gpi
Infectivity
Lit
Lit Plus Fetal Bovine Serum, Hemin, And Penicillin-Streptomycin
Lit-Hsp
Liver Infusion Tryptose Medium
Mlee
Multilocus Enzyme Electrophoresis
Random Amplified Polymorphic Dna
Rapd
Ribonucleic Acid
Rna
Selection
Tae
Tris-Acetate Edta
Trypanosoma Cruzi
title_short Trypanosoma cruzi: Infectivity modulation of a clone after passages through different hosts
title_full Trypanosoma cruzi: Infectivity modulation of a clone after passages through different hosts
title_fullStr Trypanosoma cruzi: Infectivity modulation of a clone after passages through different hosts
title_full_unstemmed Trypanosoma cruzi: Infectivity modulation of a clone after passages through different hosts
title_sort Trypanosoma cruzi: Infectivity modulation of a clone after passages through different hosts
dc.creator.none.fl_str_mv Pérez Brandan, Cecilia María
Padilla, Ángel Marcelo
Diosque, Patricio
Basombrío, Miguel Ángel Manuel
author Pérez Brandan, Cecilia María
author_facet Pérez Brandan, Cecilia María
Padilla, Ángel Marcelo
Diosque, Patricio
Basombrío, Miguel Ángel Manuel
author_role author
author2 Padilla, Ángel Marcelo
Diosque, Patricio
Basombrío, Miguel Ángel Manuel
author2_role author
author
author
dc.subject.none.fl_str_mv Clone Line from Tolac1 Isolate
Clones
Cta1
Deoxyribonucleic Acid
Dna
Glucose-6-Phosphate Isomerase
Gpi
Infectivity
Lit
Lit Plus Fetal Bovine Serum, Hemin, And Penicillin-Streptomycin
Lit-Hsp
Liver Infusion Tryptose Medium
Mlee
Multilocus Enzyme Electrophoresis
Random Amplified Polymorphic Dna
Rapd
Ribonucleic Acid
Rna
Selection
Tae
Tris-Acetate Edta
Trypanosoma Cruzi
topic Clone Line from Tolac1 Isolate
Clones
Cta1
Deoxyribonucleic Acid
Dna
Glucose-6-Phosphate Isomerase
Gpi
Infectivity
Lit
Lit Plus Fetal Bovine Serum, Hemin, And Penicillin-Streptomycin
Lit-Hsp
Liver Infusion Tryptose Medium
Mlee
Multilocus Enzyme Electrophoresis
Random Amplified Polymorphic Dna
Rapd
Ribonucleic Acid
Rna
Selection
Tae
Tris-Acetate Edta
Trypanosoma Cruzi
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Although Trypanosoma cruzi virulence can be modified through passages in vivo or long-term in vitro culture, the mechanisms involved are poorly understood. Here we report modifications in the infectivity of a T. cruzi clone after passages in different hosts without detectable changes in parasite genetic patterns. A clone was obtained from a T. cruzi IIe isolate and showed to be less virulent than the original isolate (p < 0.05). This clone was enzymatically similar to the original isolate as shown by multilocus enzyme electrophoresis. Infection of this clone was compared by successive passages in mice and guinea pigs. The mouse-passaged subline became more virulent for both host species compared to the guinea pig-passaged subline (p < 0.05). The clone line displayed similar random amplified polymorphic DNA patterns before and after passages in different hosts suggesting that alterations in virulence could be a result of a differential expression of virulence factors.
Fil: Pérez Brandan, Cecilia María. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Padilla, Ángel Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina
Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina
Fil: Basombrío, Miguel Ángel Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta; Argentina
description Although Trypanosoma cruzi virulence can be modified through passages in vivo or long-term in vitro culture, the mechanisms involved are poorly understood. Here we report modifications in the infectivity of a T. cruzi clone after passages in different hosts without detectable changes in parasite genetic patterns. A clone was obtained from a T. cruzi IIe isolate and showed to be less virulent than the original isolate (p < 0.05). This clone was enzymatically similar to the original isolate as shown by multilocus enzyme electrophoresis. Infection of this clone was compared by successive passages in mice and guinea pigs. The mouse-passaged subline became more virulent for both host species compared to the guinea pig-passaged subline (p < 0.05). The clone line displayed similar random amplified polymorphic DNA patterns before and after passages in different hosts suggesting that alterations in virulence could be a result of a differential expression of virulence factors.
publishDate 2006
dc.date.none.fl_str_mv 2006-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36596
Pérez Brandan, Cecilia María; Padilla, Ángel Marcelo; Diosque, Patricio; Basombrío, Miguel Ángel Manuel; Trypanosoma cruzi: Infectivity modulation of a clone after passages through different hosts; Elsevier; Experimental Parasitology; 114; 2; 10-2006; 89-93
0014-4894
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36596
identifier_str_mv Pérez Brandan, Cecilia María; Padilla, Ángel Marcelo; Diosque, Patricio; Basombrío, Miguel Ángel Manuel; Trypanosoma cruzi: Infectivity modulation of a clone after passages through different hosts; Elsevier; Experimental Parasitology; 114; 2; 10-2006; 89-93
0014-4894
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.exppara.2006.02.014
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014489406000439
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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