The isolation and molecular characterization of Leishmania spp. from patients with American tegumentary leishmaniasis in northwest Argentina

Autores
Locatelli, Fabricio M.; Cajal, S. Pamela; Barroso, Paola Andrea; Lauthier, Juan José; Mora, Maria Celia; Juarez, Marisa; Kato, Hirotomo; Nasser, Julio R.; Hashiguchi, Yoshihisa; Korenaga, Masataka; Marco, Jorge Diego
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
American tegumentary leishmaniasis (ATL) is a group of zoonotic diseases caused by kinetoplastid flagellates of the genus Leishmania. A total of 66 patients diagnosed as positive ATL cases from northwest Argentina were included in this study. Leishmania stocks were isolated in vitro and analyzed over promastigote cultures sown on FTA through nested PCR and sequence of cytochrome b (cyt b). The molecular analysis resulted in the incrimination of L. (Viannia) braziliensis as the predominant species in the studied area, identifying two genotypes of L. (V.) braziliensis, 24 cases of Ab-1 cyt b and 41 cases of Ab-2 cyt b. One L. (V.) guyanensis strain was obtained from a traveler from the Brazilian Amazon. The prevalence of different genotypes was in agreement with previous studies, suggesting the necessity for new systems to study the genetic diversity in more detail. Most of the cases typified in this study were registered in the area of Zenta Valley (Orán, Hipólito Yrigoyen, and Pichanal cities), pointing a link between genotype and geographical origin of the sample. Sex and age distribution of the patients indicate that the transmission was predominantly associated with rural areas or rural activities, although the results might not exclude the possibility of peri-urban transmission. This work represents, so far, the largest isolation and molecular characterization of ATL cases in Argentina
Fil: Locatelli, Fabricio M.. Kochi University. Kochi Medical School. Dept.of Parasitology; Japón. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Cajal, S. Pamela. Universidad Nacional de Salta. Instituto de Investigaciones en Enfermedades Tropicales; Argentina
Fil: Barroso, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Lauthier, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina
Fil: Mora, Maria Celia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina
Fil: Juarez, Marisa. Universidad Nacional de Salta. Instituto de Investigaciones en Enfermedades Tropicales; Argentina
Fil: Kato, Hirotomo. Hokkaido University; Japón
Fil: Nasser, Julio R.. Universidad Nacional de Salta. Instituto de Investigaciones en Enfermedades Tropicales; Argentina
Fil: Hashiguchi, Yoshihisa. Universidad Central del Ecuador y Proyecto Prometeo; Ecuador. Kochi University. Kochi Medical School. Dept.of Parasitology; Japón
Fil: Korenaga, Masataka. Kochi University. Kochi Medical School. Dept.of Parasitology; Japón
Fil: Marco, Jorge Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina
Materia
Leishmania
Tipificación Molecular
Citocromo B
Argentina
American Tegumentary Leishmaniasis
Genotyping
Leishmania (V) Braziliensis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7020
Acceder
id CONICETDig_5182112208c2fcb5c4dca38f135f04b3
oai_identifier_str oai:ri.conicet.gov.ar:11336/7020
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The isolation and molecular characterization of Leishmania spp. from patients with American tegumentary leishmaniasis in northwest ArgentinaLocatelli, Fabricio M.Cajal, S. PamelaBarroso, Paola AndreaLauthier, Juan JoséMora, Maria CeliaJuarez, MarisaKato, HirotomoNasser, Julio R.Hashiguchi, YoshihisaKorenaga, MasatakaMarco, Jorge DiegoLeishmaniaTipificación MolecularCitocromo BArgentinaAmerican Tegumentary LeishmaniasisGenotypingLeishmania (V) Braziliensishttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3American tegumentary leishmaniasis (ATL) is a group of zoonotic diseases caused by kinetoplastid flagellates of the genus Leishmania. A total of 66 patients diagnosed as positive ATL cases from northwest Argentina were included in this study. Leishmania stocks were isolated in vitro and analyzed over promastigote cultures sown on FTA through nested PCR and sequence of cytochrome b (cyt b). The molecular analysis resulted in the incrimination of L. (Viannia) braziliensis as the predominant species in the studied area, identifying two genotypes of L. (V.) braziliensis, 24 cases of Ab-1 cyt b and 41 cases of Ab-2 cyt b. One L. (V.) guyanensis strain was obtained from a traveler from the Brazilian Amazon. The prevalence of different genotypes was in agreement with previous studies, suggesting the necessity for new systems to study the genetic diversity in more detail. Most of the cases typified in this study were registered in the area of Zenta Valley (Orán, Hipólito Yrigoyen, and Pichanal cities), pointing a link between genotype and geographical origin of the sample. Sex and age distribution of the patients indicate that the transmission was predominantly associated with rural areas or rural activities, although the results might not exclude the possibility of peri-urban transmission. This work represents, so far, the largest isolation and molecular characterization of ATL cases in ArgentinaFil: Locatelli, Fabricio M.. Kochi University. Kochi Medical School. Dept.of Parasitology; Japón. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Cajal, S. Pamela. Universidad Nacional de Salta. Instituto de Investigaciones en Enfermedades Tropicales; ArgentinaFil: Barroso, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Lauthier, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; ArgentinaFil: Mora, Maria Celia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; ArgentinaFil: Juarez, Marisa. Universidad Nacional de Salta. Instituto de Investigaciones en Enfermedades Tropicales; ArgentinaFil: Kato, Hirotomo. Hokkaido University; JapónFil: Nasser, Julio R.. Universidad Nacional de Salta. Instituto de Investigaciones en Enfermedades Tropicales; ArgentinaFil: Hashiguchi, Yoshihisa. Universidad Central del Ecuador y Proyecto Prometeo; Ecuador. Kochi University. Kochi Medical School. Dept.of Parasitology; JapónFil: Korenaga, Masataka. Kochi University. Kochi Medical School. Dept.of Parasitology; JapónFil: Marco, Jorge Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; ArgentinaElsevier Science2013-11-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7020Locatelli, Fabricio M.; Cajal, S. Pamela; Barroso, Paola Andrea; Lauthier, Juan José; Mora, Maria Celia; et al.; The isolation and molecular characterization of Leishmania spp. from patients with American tegumentary leishmaniasis in northwest Argentina; Elsevier Science; Acta Tropica; 131; 27-11-2013; 16-210001-706Xenginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.actatropica.2013.11.015info:eu-repo/semantics/altIdentifier/pmid/24291551info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0001706X13003380info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:49:55Zoai:ri.conicet.gov.ar:11336/7020instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:49:56.277CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The isolation and molecular characterization of Leishmania spp. from patients with American tegumentary leishmaniasis in northwest Argentina
title The isolation and molecular characterization of Leishmania spp. from patients with American tegumentary leishmaniasis in northwest Argentina
spellingShingle The isolation and molecular characterization of Leishmania spp. from patients with American tegumentary leishmaniasis in northwest Argentina
Locatelli, Fabricio M.
Leishmania
Tipificación Molecular
Citocromo B
Argentina
American Tegumentary Leishmaniasis
Genotyping
Leishmania (V) Braziliensis
title_short The isolation and molecular characterization of Leishmania spp. from patients with American tegumentary leishmaniasis in northwest Argentina
title_full The isolation and molecular characterization of Leishmania spp. from patients with American tegumentary leishmaniasis in northwest Argentina
title_fullStr The isolation and molecular characterization of Leishmania spp. from patients with American tegumentary leishmaniasis in northwest Argentina
title_full_unstemmed The isolation and molecular characterization of Leishmania spp. from patients with American tegumentary leishmaniasis in northwest Argentina
title_sort The isolation and molecular characterization of Leishmania spp. from patients with American tegumentary leishmaniasis in northwest Argentina
dc.creator.none.fl_str_mv Locatelli, Fabricio M.
Cajal, S. Pamela
Barroso, Paola Andrea
Lauthier, Juan José
Mora, Maria Celia
Juarez, Marisa
Kato, Hirotomo
Nasser, Julio R.
Hashiguchi, Yoshihisa
Korenaga, Masataka
Marco, Jorge Diego
author Locatelli, Fabricio M.
author_facet Locatelli, Fabricio M.
Cajal, S. Pamela
Barroso, Paola Andrea
Lauthier, Juan José
Mora, Maria Celia
Juarez, Marisa
Kato, Hirotomo
Nasser, Julio R.
Hashiguchi, Yoshihisa
Korenaga, Masataka
Marco, Jorge Diego
author_role author
author2 Cajal, S. Pamela
Barroso, Paola Andrea
Lauthier, Juan José
Mora, Maria Celia
Juarez, Marisa
Kato, Hirotomo
Nasser, Julio R.
Hashiguchi, Yoshihisa
Korenaga, Masataka
Marco, Jorge Diego
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Leishmania
Tipificación Molecular
Citocromo B
Argentina
American Tegumentary Leishmaniasis
Genotyping
Leishmania (V) Braziliensis
topic Leishmania
Tipificación Molecular
Citocromo B
Argentina
American Tegumentary Leishmaniasis
Genotyping
Leishmania (V) Braziliensis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv American tegumentary leishmaniasis (ATL) is a group of zoonotic diseases caused by kinetoplastid flagellates of the genus Leishmania. A total of 66 patients diagnosed as positive ATL cases from northwest Argentina were included in this study. Leishmania stocks were isolated in vitro and analyzed over promastigote cultures sown on FTA through nested PCR and sequence of cytochrome b (cyt b). The molecular analysis resulted in the incrimination of L. (Viannia) braziliensis as the predominant species in the studied area, identifying two genotypes of L. (V.) braziliensis, 24 cases of Ab-1 cyt b and 41 cases of Ab-2 cyt b. One L. (V.) guyanensis strain was obtained from a traveler from the Brazilian Amazon. The prevalence of different genotypes was in agreement with previous studies, suggesting the necessity for new systems to study the genetic diversity in more detail. Most of the cases typified in this study were registered in the area of Zenta Valley (Orán, Hipólito Yrigoyen, and Pichanal cities), pointing a link between genotype and geographical origin of the sample. Sex and age distribution of the patients indicate that the transmission was predominantly associated with rural areas or rural activities, although the results might not exclude the possibility of peri-urban transmission. This work represents, so far, the largest isolation and molecular characterization of ATL cases in Argentina
Fil: Locatelli, Fabricio M.. Kochi University. Kochi Medical School. Dept.of Parasitology; Japón. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Cajal, S. Pamela. Universidad Nacional de Salta. Instituto de Investigaciones en Enfermedades Tropicales; Argentina
Fil: Barroso, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Lauthier, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina
Fil: Mora, Maria Celia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina
Fil: Juarez, Marisa. Universidad Nacional de Salta. Instituto de Investigaciones en Enfermedades Tropicales; Argentina
Fil: Kato, Hirotomo. Hokkaido University; Japón
Fil: Nasser, Julio R.. Universidad Nacional de Salta. Instituto de Investigaciones en Enfermedades Tropicales; Argentina
Fil: Hashiguchi, Yoshihisa. Universidad Central del Ecuador y Proyecto Prometeo; Ecuador. Kochi University. Kochi Medical School. Dept.of Parasitology; Japón
Fil: Korenaga, Masataka. Kochi University. Kochi Medical School. Dept.of Parasitology; Japón
Fil: Marco, Jorge Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina
description American tegumentary leishmaniasis (ATL) is a group of zoonotic diseases caused by kinetoplastid flagellates of the genus Leishmania. A total of 66 patients diagnosed as positive ATL cases from northwest Argentina were included in this study. Leishmania stocks were isolated in vitro and analyzed over promastigote cultures sown on FTA through nested PCR and sequence of cytochrome b (cyt b). The molecular analysis resulted in the incrimination of L. (Viannia) braziliensis as the predominant species in the studied area, identifying two genotypes of L. (V.) braziliensis, 24 cases of Ab-1 cyt b and 41 cases of Ab-2 cyt b. One L. (V.) guyanensis strain was obtained from a traveler from the Brazilian Amazon. The prevalence of different genotypes was in agreement with previous studies, suggesting the necessity for new systems to study the genetic diversity in more detail. Most of the cases typified in this study were registered in the area of Zenta Valley (Orán, Hipólito Yrigoyen, and Pichanal cities), pointing a link between genotype and geographical origin of the sample. Sex and age distribution of the patients indicate that the transmission was predominantly associated with rural areas or rural activities, although the results might not exclude the possibility of peri-urban transmission. This work represents, so far, the largest isolation and molecular characterization of ATL cases in Argentina
publishDate 2013
dc.date.none.fl_str_mv 2013-11-27
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7020
Locatelli, Fabricio M.; Cajal, S. Pamela; Barroso, Paola Andrea; Lauthier, Juan José; Mora, Maria Celia; et al.; The isolation and molecular characterization of Leishmania spp. from patients with American tegumentary leishmaniasis in northwest Argentina; Elsevier Science; Acta Tropica; 131; 27-11-2013; 16-21
0001-706X
url http://hdl.handle.net/11336/7020
identifier_str_mv Locatelli, Fabricio M.; Cajal, S. Pamela; Barroso, Paola Andrea; Lauthier, Juan José; Mora, Maria Celia; et al.; The isolation and molecular characterization of Leishmania spp. from patients with American tegumentary leishmaniasis in northwest Argentina; Elsevier Science; Acta Tropica; 131; 27-11-2013; 16-21
0001-706X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.actatropica.2013.11.015
info:eu-repo/semantics/altIdentifier/pmid/24291551
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0001706X13003380
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083023116697600
score 13.22299

Publicaciones similares