First insights on the retroelement Rex1 in the cytogenetics of frogs
- Autores
- Nascimento, Juliana; Baldo, Juan Diego; Lourenço, Lucina
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: While some transposable elements (TEs) have been found in the sequenced genomes of frog species, detailed studies of these elements have been lacking. In this work, we investigated the occurrence of the Rex1 element, which is widespread in fish, in anurans of the genus Physalaemus. We isolated and characterized the reverse transcriptase (RT)-coding sequences of Rex1 elements of five species of this genus. Results: The amino acid sequences deduced from the nucleotide sequences of the isolated fragments allowed us to unambiguously identify regions corresponding to domains 3-7 of RT. Some of the nucleotide sequences isolated from Physlaemus ephippifer and P. albonotatus had internal deletions, suggesting that these fragments are likely not active TEs, despite being derived from a Rex1 element. When hybridized with metaphase chromosomes, Rex1 probes were revealed at the pericentromeric heterochromatic region of the short arm of chromosome 3 of the P. ephippifer karyotype. Neither other heterochromatin sites of the P. ephippifer karyotype nor any chromosomal regions of the karyotypes of P. albonotatus, P. spiniger and P. albifrons were detected with these probes. Conclusions: Rex1 elements were found in the genomes of five species of Physalaemus but clustered in only the P. ephippifer karyotype, in contrast to observations in some species of fish, where large chromosomal sites with Rex1 elements are typically present.
Fil: Nascimento, Juliana. Universidade Estadual de Campinas; Brasil
Fil: Baldo, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Lourenço, Lucina. Universidade Estadual de Campinas; Brasil - Materia
-
CHROMOSOME
IN SITU HYBRIDIZATION
LEPTODACTYLIDAE
REPETITIVE DNA
RETROTRANSPOSON - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/37562
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
spelling |
First insights on the retroelement Rex1 in the cytogenetics of frogsNascimento, JulianaBaldo, Juan DiegoLourenço, LucinaCHROMOSOMEIN SITU HYBRIDIZATIONLEPTODACTYLIDAEREPETITIVE DNARETROTRANSPOSONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: While some transposable elements (TEs) have been found in the sequenced genomes of frog species, detailed studies of these elements have been lacking. In this work, we investigated the occurrence of the Rex1 element, which is widespread in fish, in anurans of the genus Physalaemus. We isolated and characterized the reverse transcriptase (RT)-coding sequences of Rex1 elements of five species of this genus. Results: The amino acid sequences deduced from the nucleotide sequences of the isolated fragments allowed us to unambiguously identify regions corresponding to domains 3-7 of RT. Some of the nucleotide sequences isolated from Physlaemus ephippifer and P. albonotatus had internal deletions, suggesting that these fragments are likely not active TEs, despite being derived from a Rex1 element. When hybridized with metaphase chromosomes, Rex1 probes were revealed at the pericentromeric heterochromatic region of the short arm of chromosome 3 of the P. ephippifer karyotype. Neither other heterochromatin sites of the P. ephippifer karyotype nor any chromosomal regions of the karyotypes of P. albonotatus, P. spiniger and P. albifrons were detected with these probes. Conclusions: Rex1 elements were found in the genomes of five species of Physalaemus but clustered in only the P. ephippifer karyotype, in contrast to observations in some species of fish, where large chromosomal sites with Rex1 elements are typically present.Fil: Nascimento, Juliana. Universidade Estadual de Campinas; BrasilFil: Baldo, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Lourenço, Lucina. Universidade Estadual de Campinas; BrasilBioMed Central Ltd.2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37562Nascimento, Juliana; Baldo, Juan Diego; Lourenço, Lucina; First insights on the retroelement Rex1 in the cytogenetics of frogs; BioMed Central Ltd.; Molecular Cytogenetics; 8; 86; 11-2015; 1-91755-81661755-8166CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s13039-015-0189-5info:eu-repo/semantics/altIdentifier/url/https://molecularcytogenetics.biomedcentral.com/articles/10.1186/s13039-015-0189-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:21:48Zoai:ri.conicet.gov.ar:11336/37562instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:21:48.679CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
First insights on the retroelement Rex1 in the cytogenetics of frogs |
title |
First insights on the retroelement Rex1 in the cytogenetics of frogs |
spellingShingle |
First insights on the retroelement Rex1 in the cytogenetics of frogs Nascimento, Juliana CHROMOSOME IN SITU HYBRIDIZATION LEPTODACTYLIDAE REPETITIVE DNA RETROTRANSPOSON |
title_short |
First insights on the retroelement Rex1 in the cytogenetics of frogs |
title_full |
First insights on the retroelement Rex1 in the cytogenetics of frogs |
title_fullStr |
First insights on the retroelement Rex1 in the cytogenetics of frogs |
title_full_unstemmed |
First insights on the retroelement Rex1 in the cytogenetics of frogs |
title_sort |
First insights on the retroelement Rex1 in the cytogenetics of frogs |
dc.creator.none.fl_str_mv |
Nascimento, Juliana Baldo, Juan Diego Lourenço, Lucina |
author |
Nascimento, Juliana |
author_facet |
Nascimento, Juliana Baldo, Juan Diego Lourenço, Lucina |
author_role |
author |
author2 |
Baldo, Juan Diego Lourenço, Lucina |
author2_role |
author author |
dc.subject.none.fl_str_mv |
CHROMOSOME IN SITU HYBRIDIZATION LEPTODACTYLIDAE REPETITIVE DNA RETROTRANSPOSON |
topic |
CHROMOSOME IN SITU HYBRIDIZATION LEPTODACTYLIDAE REPETITIVE DNA RETROTRANSPOSON |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Background: While some transposable elements (TEs) have been found in the sequenced genomes of frog species, detailed studies of these elements have been lacking. In this work, we investigated the occurrence of the Rex1 element, which is widespread in fish, in anurans of the genus Physalaemus. We isolated and characterized the reverse transcriptase (RT)-coding sequences of Rex1 elements of five species of this genus. Results: The amino acid sequences deduced from the nucleotide sequences of the isolated fragments allowed us to unambiguously identify regions corresponding to domains 3-7 of RT. Some of the nucleotide sequences isolated from Physlaemus ephippifer and P. albonotatus had internal deletions, suggesting that these fragments are likely not active TEs, despite being derived from a Rex1 element. When hybridized with metaphase chromosomes, Rex1 probes were revealed at the pericentromeric heterochromatic region of the short arm of chromosome 3 of the P. ephippifer karyotype. Neither other heterochromatin sites of the P. ephippifer karyotype nor any chromosomal regions of the karyotypes of P. albonotatus, P. spiniger and P. albifrons were detected with these probes. Conclusions: Rex1 elements were found in the genomes of five species of Physalaemus but clustered in only the P. ephippifer karyotype, in contrast to observations in some species of fish, where large chromosomal sites with Rex1 elements are typically present. Fil: Nascimento, Juliana. Universidade Estadual de Campinas; Brasil Fil: Baldo, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina Fil: Lourenço, Lucina. Universidade Estadual de Campinas; Brasil |
description |
Background: While some transposable elements (TEs) have been found in the sequenced genomes of frog species, detailed studies of these elements have been lacking. In this work, we investigated the occurrence of the Rex1 element, which is widespread in fish, in anurans of the genus Physalaemus. We isolated and characterized the reverse transcriptase (RT)-coding sequences of Rex1 elements of five species of this genus. Results: The amino acid sequences deduced from the nucleotide sequences of the isolated fragments allowed us to unambiguously identify regions corresponding to domains 3-7 of RT. Some of the nucleotide sequences isolated from Physlaemus ephippifer and P. albonotatus had internal deletions, suggesting that these fragments are likely not active TEs, despite being derived from a Rex1 element. When hybridized with metaphase chromosomes, Rex1 probes were revealed at the pericentromeric heterochromatic region of the short arm of chromosome 3 of the P. ephippifer karyotype. Neither other heterochromatin sites of the P. ephippifer karyotype nor any chromosomal regions of the karyotypes of P. albonotatus, P. spiniger and P. albifrons were detected with these probes. Conclusions: Rex1 elements were found in the genomes of five species of Physalaemus but clustered in only the P. ephippifer karyotype, in contrast to observations in some species of fish, where large chromosomal sites with Rex1 elements are typically present. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/37562 Nascimento, Juliana; Baldo, Juan Diego; Lourenço, Lucina; First insights on the retroelement Rex1 in the cytogenetics of frogs; BioMed Central Ltd.; Molecular Cytogenetics; 8; 86; 11-2015; 1-9 1755-8166 1755-8166 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/37562 |
identifier_str_mv |
Nascimento, Juliana; Baldo, Juan Diego; Lourenço, Lucina; First insights on the retroelement Rex1 in the cytogenetics of frogs; BioMed Central Ltd.; Molecular Cytogenetics; 8; 86; 11-2015; 1-9 1755-8166 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1186/s13039-015-0189-5 info:eu-repo/semantics/altIdentifier/url/https://molecularcytogenetics.biomedcentral.com/articles/10.1186/s13039-015-0189-5 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
BioMed Central Ltd. |
publisher.none.fl_str_mv |
BioMed Central Ltd. |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |