First insights on the retroelement Rex1 in the cytogenetics of frogs

Autores
Nascimento, Juliana; Baldo, Juan Diego; Lourenço, Lucina
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: While some transposable elements (TEs) have been found in the sequenced genomes of frog species, detailed studies of these elements have been lacking. In this work, we investigated the occurrence of the Rex1 element, which is widespread in fish, in anurans of the genus Physalaemus. We isolated and characterized the reverse transcriptase (RT)-coding sequences of Rex1 elements of five species of this genus. Results: The amino acid sequences deduced from the nucleotide sequences of the isolated fragments allowed us to unambiguously identify regions corresponding to domains 3-7 of RT. Some of the nucleotide sequences isolated from Physlaemus ephippifer and P. albonotatus had internal deletions, suggesting that these fragments are likely not active TEs, despite being derived from a Rex1 element. When hybridized with metaphase chromosomes, Rex1 probes were revealed at the pericentromeric heterochromatic region of the short arm of chromosome 3 of the P. ephippifer karyotype. Neither other heterochromatin sites of the P. ephippifer karyotype nor any chromosomal regions of the karyotypes of P. albonotatus, P. spiniger and P. albifrons were detected with these probes. Conclusions: Rex1 elements were found in the genomes of five species of Physalaemus but clustered in only the P. ephippifer karyotype, in contrast to observations in some species of fish, where large chromosomal sites with Rex1 elements are typically present.
Fil: Nascimento, Juliana. Universidade Estadual de Campinas; Brasil
Fil: Baldo, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Lourenço, Lucina. Universidade Estadual de Campinas; Brasil
Materia
CHROMOSOME
IN SITU HYBRIDIZATION
LEPTODACTYLIDAE
REPETITIVE DNA
RETROTRANSPOSON
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37562

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network_name_str CONICET Digital (CONICET)
spelling First insights on the retroelement Rex1 in the cytogenetics of frogsNascimento, JulianaBaldo, Juan DiegoLourenço, LucinaCHROMOSOMEIN SITU HYBRIDIZATIONLEPTODACTYLIDAEREPETITIVE DNARETROTRANSPOSONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: While some transposable elements (TEs) have been found in the sequenced genomes of frog species, detailed studies of these elements have been lacking. In this work, we investigated the occurrence of the Rex1 element, which is widespread in fish, in anurans of the genus Physalaemus. We isolated and characterized the reverse transcriptase (RT)-coding sequences of Rex1 elements of five species of this genus. Results: The amino acid sequences deduced from the nucleotide sequences of the isolated fragments allowed us to unambiguously identify regions corresponding to domains 3-7 of RT. Some of the nucleotide sequences isolated from Physlaemus ephippifer and P. albonotatus had internal deletions, suggesting that these fragments are likely not active TEs, despite being derived from a Rex1 element. When hybridized with metaphase chromosomes, Rex1 probes were revealed at the pericentromeric heterochromatic region of the short arm of chromosome 3 of the P. ephippifer karyotype. Neither other heterochromatin sites of the P. ephippifer karyotype nor any chromosomal regions of the karyotypes of P. albonotatus, P. spiniger and P. albifrons were detected with these probes. Conclusions: Rex1 elements were found in the genomes of five species of Physalaemus but clustered in only the P. ephippifer karyotype, in contrast to observations in some species of fish, where large chromosomal sites with Rex1 elements are typically present.Fil: Nascimento, Juliana. Universidade Estadual de Campinas; BrasilFil: Baldo, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Lourenço, Lucina. Universidade Estadual de Campinas; BrasilBioMed Central Ltd.2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37562Nascimento, Juliana; Baldo, Juan Diego; Lourenço, Lucina; First insights on the retroelement Rex1 in the cytogenetics of frogs; BioMed Central Ltd.; Molecular Cytogenetics; 8; 86; 11-2015; 1-91755-81661755-8166CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s13039-015-0189-5info:eu-repo/semantics/altIdentifier/url/https://molecularcytogenetics.biomedcentral.com/articles/10.1186/s13039-015-0189-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:21:48Zoai:ri.conicet.gov.ar:11336/37562instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:21:48.679CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First insights on the retroelement Rex1 in the cytogenetics of frogs
title First insights on the retroelement Rex1 in the cytogenetics of frogs
spellingShingle First insights on the retroelement Rex1 in the cytogenetics of frogs
Nascimento, Juliana
CHROMOSOME
IN SITU HYBRIDIZATION
LEPTODACTYLIDAE
REPETITIVE DNA
RETROTRANSPOSON
title_short First insights on the retroelement Rex1 in the cytogenetics of frogs
title_full First insights on the retroelement Rex1 in the cytogenetics of frogs
title_fullStr First insights on the retroelement Rex1 in the cytogenetics of frogs
title_full_unstemmed First insights on the retroelement Rex1 in the cytogenetics of frogs
title_sort First insights on the retroelement Rex1 in the cytogenetics of frogs
dc.creator.none.fl_str_mv Nascimento, Juliana
Baldo, Juan Diego
Lourenço, Lucina
author Nascimento, Juliana
author_facet Nascimento, Juliana
Baldo, Juan Diego
Lourenço, Lucina
author_role author
author2 Baldo, Juan Diego
Lourenço, Lucina
author2_role author
author
dc.subject.none.fl_str_mv CHROMOSOME
IN SITU HYBRIDIZATION
LEPTODACTYLIDAE
REPETITIVE DNA
RETROTRANSPOSON
topic CHROMOSOME
IN SITU HYBRIDIZATION
LEPTODACTYLIDAE
REPETITIVE DNA
RETROTRANSPOSON
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: While some transposable elements (TEs) have been found in the sequenced genomes of frog species, detailed studies of these elements have been lacking. In this work, we investigated the occurrence of the Rex1 element, which is widespread in fish, in anurans of the genus Physalaemus. We isolated and characterized the reverse transcriptase (RT)-coding sequences of Rex1 elements of five species of this genus. Results: The amino acid sequences deduced from the nucleotide sequences of the isolated fragments allowed us to unambiguously identify regions corresponding to domains 3-7 of RT. Some of the nucleotide sequences isolated from Physlaemus ephippifer and P. albonotatus had internal deletions, suggesting that these fragments are likely not active TEs, despite being derived from a Rex1 element. When hybridized with metaphase chromosomes, Rex1 probes were revealed at the pericentromeric heterochromatic region of the short arm of chromosome 3 of the P. ephippifer karyotype. Neither other heterochromatin sites of the P. ephippifer karyotype nor any chromosomal regions of the karyotypes of P. albonotatus, P. spiniger and P. albifrons were detected with these probes. Conclusions: Rex1 elements were found in the genomes of five species of Physalaemus but clustered in only the P. ephippifer karyotype, in contrast to observations in some species of fish, where large chromosomal sites with Rex1 elements are typically present.
Fil: Nascimento, Juliana. Universidade Estadual de Campinas; Brasil
Fil: Baldo, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Lourenço, Lucina. Universidade Estadual de Campinas; Brasil
description Background: While some transposable elements (TEs) have been found in the sequenced genomes of frog species, detailed studies of these elements have been lacking. In this work, we investigated the occurrence of the Rex1 element, which is widespread in fish, in anurans of the genus Physalaemus. We isolated and characterized the reverse transcriptase (RT)-coding sequences of Rex1 elements of five species of this genus. Results: The amino acid sequences deduced from the nucleotide sequences of the isolated fragments allowed us to unambiguously identify regions corresponding to domains 3-7 of RT. Some of the nucleotide sequences isolated from Physlaemus ephippifer and P. albonotatus had internal deletions, suggesting that these fragments are likely not active TEs, despite being derived from a Rex1 element. When hybridized with metaphase chromosomes, Rex1 probes were revealed at the pericentromeric heterochromatic region of the short arm of chromosome 3 of the P. ephippifer karyotype. Neither other heterochromatin sites of the P. ephippifer karyotype nor any chromosomal regions of the karyotypes of P. albonotatus, P. spiniger and P. albifrons were detected with these probes. Conclusions: Rex1 elements were found in the genomes of five species of Physalaemus but clustered in only the P. ephippifer karyotype, in contrast to observations in some species of fish, where large chromosomal sites with Rex1 elements are typically present.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37562
Nascimento, Juliana; Baldo, Juan Diego; Lourenço, Lucina; First insights on the retroelement Rex1 in the cytogenetics of frogs; BioMed Central Ltd.; Molecular Cytogenetics; 8; 86; 11-2015; 1-9
1755-8166
1755-8166
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37562
identifier_str_mv Nascimento, Juliana; Baldo, Juan Diego; Lourenço, Lucina; First insights on the retroelement Rex1 in the cytogenetics of frogs; BioMed Central Ltd.; Molecular Cytogenetics; 8; 86; 11-2015; 1-9
1755-8166
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s13039-015-0189-5
info:eu-repo/semantics/altIdentifier/url/https://molecularcytogenetics.biomedcentral.com/articles/10.1186/s13039-015-0189-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central Ltd.
publisher.none.fl_str_mv BioMed Central Ltd.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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