Neo-sex chromosomes of Ronderosia bergi: insight into the evolution of sex chromosomes in grasshoppers

Autores
Palacios Gimenez, O. M.; Marti, Dardo Andrea; Cabral de Mello, D. C.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sex chromosomes have evolved many times from morphologically identical autosome pairs, most often presenting several recombination suppression events, followed by accumulation of repetitive DNA sequences. In Orthoptera, most species have an X0♂ sex chromosome system. However, in the subfamily Melanoplinae, derived variants of neo-sex chromosomes (neo-XY♂ or neo-X1X2Y♂) emerged several times. Here, we examined the differentiation of neo-sex chromosomes in a Melanoplinae species with a neo-XY♂/XX♀ system, Ronderosia bergi, using several approaches: (i) classical cytogenetic analysis, (ii) mapping via fluorescent in situ hybridization of some selected repetitive DNA sequences and microdissected sex chromosomes, and (iii) immunolocalization of distinct histone modifications. The microdissected sex chromosomes were also used as sources for Polymerase chain reaction (PCR) amplification of RNA-coding multigene families, to study variants related to the sex chromosomes. Our data suggest that the R. bergi neo-Y has become differentiated after its formation by a Robertsonian translocation and inversions, and has accumulated repetitive DNA sequences. Interestingly, the ex autosomes incorporated into the neo-sex chromosomes retain some autosomal post-translational histone modifications, at least in metaphase I, suggesting that the establishment of functional modifications in neo-sex chromosomes is slower than their sequence differentiation.
Fil: Palacios Gimenez, O. M.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Marti, Dardo Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Cabral de Mello, D. C.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Materia
Fish
Histone Modification
Neo-Sex Chromosomes
Rb-Translocation
Repetitive Dna
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37313
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Neo-sex chromosomes of Ronderosia bergi: insight into the evolution of sex chromosomes in grasshoppersPalacios Gimenez, O. M.Marti, Dardo AndreaCabral de Mello, D. C.FishHistone ModificationNeo-Sex ChromosomesRb-TranslocationRepetitive Dnahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sex chromosomes have evolved many times from morphologically identical autosome pairs, most often presenting several recombination suppression events, followed by accumulation of repetitive DNA sequences. In Orthoptera, most species have an X0♂ sex chromosome system. However, in the subfamily Melanoplinae, derived variants of neo-sex chromosomes (neo-XY♂ or neo-X1X2Y♂) emerged several times. Here, we examined the differentiation of neo-sex chromosomes in a Melanoplinae species with a neo-XY♂/XX♀ system, Ronderosia bergi, using several approaches: (i) classical cytogenetic analysis, (ii) mapping via fluorescent in situ hybridization of some selected repetitive DNA sequences and microdissected sex chromosomes, and (iii) immunolocalization of distinct histone modifications. The microdissected sex chromosomes were also used as sources for Polymerase chain reaction (PCR) amplification of RNA-coding multigene families, to study variants related to the sex chromosomes. Our data suggest that the R. bergi neo-Y has become differentiated after its formation by a Robertsonian translocation and inversions, and has accumulated repetitive DNA sequences. Interestingly, the ex autosomes incorporated into the neo-sex chromosomes retain some autosomal post-translational histone modifications, at least in metaphase I, suggesting that the establishment of functional modifications in neo-sex chromosomes is slower than their sequence differentiation.Fil: Palacios Gimenez, O. M.. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Marti, Dardo Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; ArgentinaFil: Cabral de Mello, D. C.. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilSpringer2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37313Palacios Gimenez, O. M.; Marti, Dardo Andrea; Cabral de Mello, D. C.; Neo-sex chromosomes of Ronderosia bergi: insight into the evolution of sex chromosomes in grasshoppers; Springer; Chromosoma; 124; 3; 9-2015; 353-3650009-5915CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00412-015-0505-1info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00412-015-0505-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:52:36Zoai:ri.conicet.gov.ar:11336/37313instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:52:36.553CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neo-sex chromosomes of Ronderosia bergi: insight into the evolution of sex chromosomes in grasshoppers
title Neo-sex chromosomes of Ronderosia bergi: insight into the evolution of sex chromosomes in grasshoppers
spellingShingle Neo-sex chromosomes of Ronderosia bergi: insight into the evolution of sex chromosomes in grasshoppers
Palacios Gimenez, O. M.
Fish
Histone Modification
Neo-Sex Chromosomes
Rb-Translocation
Repetitive Dna
title_short Neo-sex chromosomes of Ronderosia bergi: insight into the evolution of sex chromosomes in grasshoppers
title_full Neo-sex chromosomes of Ronderosia bergi: insight into the evolution of sex chromosomes in grasshoppers
title_fullStr Neo-sex chromosomes of Ronderosia bergi: insight into the evolution of sex chromosomes in grasshoppers
title_full_unstemmed Neo-sex chromosomes of Ronderosia bergi: insight into the evolution of sex chromosomes in grasshoppers
title_sort Neo-sex chromosomes of Ronderosia bergi: insight into the evolution of sex chromosomes in grasshoppers
dc.creator.none.fl_str_mv Palacios Gimenez, O. M.
Marti, Dardo Andrea
Cabral de Mello, D. C.
author Palacios Gimenez, O. M.
author_facet Palacios Gimenez, O. M.
Marti, Dardo Andrea
Cabral de Mello, D. C.
author_role author
author2 Marti, Dardo Andrea
Cabral de Mello, D. C.
author2_role author
author
dc.subject.none.fl_str_mv Fish
Histone Modification
Neo-Sex Chromosomes
Rb-Translocation
Repetitive Dna
topic Fish
Histone Modification
Neo-Sex Chromosomes
Rb-Translocation
Repetitive Dna
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sex chromosomes have evolved many times from morphologically identical autosome pairs, most often presenting several recombination suppression events, followed by accumulation of repetitive DNA sequences. In Orthoptera, most species have an X0♂ sex chromosome system. However, in the subfamily Melanoplinae, derived variants of neo-sex chromosomes (neo-XY♂ or neo-X1X2Y♂) emerged several times. Here, we examined the differentiation of neo-sex chromosomes in a Melanoplinae species with a neo-XY♂/XX♀ system, Ronderosia bergi, using several approaches: (i) classical cytogenetic analysis, (ii) mapping via fluorescent in situ hybridization of some selected repetitive DNA sequences and microdissected sex chromosomes, and (iii) immunolocalization of distinct histone modifications. The microdissected sex chromosomes were also used as sources for Polymerase chain reaction (PCR) amplification of RNA-coding multigene families, to study variants related to the sex chromosomes. Our data suggest that the R. bergi neo-Y has become differentiated after its formation by a Robertsonian translocation and inversions, and has accumulated repetitive DNA sequences. Interestingly, the ex autosomes incorporated into the neo-sex chromosomes retain some autosomal post-translational histone modifications, at least in metaphase I, suggesting that the establishment of functional modifications in neo-sex chromosomes is slower than their sequence differentiation.
Fil: Palacios Gimenez, O. M.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Marti, Dardo Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Cabral de Mello, D. C.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
description Sex chromosomes have evolved many times from morphologically identical autosome pairs, most often presenting several recombination suppression events, followed by accumulation of repetitive DNA sequences. In Orthoptera, most species have an X0♂ sex chromosome system. However, in the subfamily Melanoplinae, derived variants of neo-sex chromosomes (neo-XY♂ or neo-X1X2Y♂) emerged several times. Here, we examined the differentiation of neo-sex chromosomes in a Melanoplinae species with a neo-XY♂/XX♀ system, Ronderosia bergi, using several approaches: (i) classical cytogenetic analysis, (ii) mapping via fluorescent in situ hybridization of some selected repetitive DNA sequences and microdissected sex chromosomes, and (iii) immunolocalization of distinct histone modifications. The microdissected sex chromosomes were also used as sources for Polymerase chain reaction (PCR) amplification of RNA-coding multigene families, to study variants related to the sex chromosomes. Our data suggest that the R. bergi neo-Y has become differentiated after its formation by a Robertsonian translocation and inversions, and has accumulated repetitive DNA sequences. Interestingly, the ex autosomes incorporated into the neo-sex chromosomes retain some autosomal post-translational histone modifications, at least in metaphase I, suggesting that the establishment of functional modifications in neo-sex chromosomes is slower than their sequence differentiation.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37313
Palacios Gimenez, O. M.; Marti, Dardo Andrea; Cabral de Mello, D. C.; Neo-sex chromosomes of Ronderosia bergi: insight into the evolution of sex chromosomes in grasshoppers; Springer; Chromosoma; 124; 3; 9-2015; 353-365
0009-5915
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37313
identifier_str_mv Palacios Gimenez, O. M.; Marti, Dardo Andrea; Cabral de Mello, D. C.; Neo-sex chromosomes of Ronderosia bergi: insight into the evolution of sex chromosomes in grasshoppers; Springer; Chromosoma; 124; 3; 9-2015; 353-365
0009-5915
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00412-015-0505-1
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00412-015-0505-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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