Characterization and changes in polyphenol oxidase from eggplant fruit (Solanum melongena L.) during storage at low temperature
- Autores
- Concellón, Analía; Añon, Maria Cristina; Chaves, Alicia Raquel
- Año de publicación
- 2004
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Polyphenoloxidase (PPO) from eggplant fruit was characterized, and its catecholase activity studied during storage of fruit at 0 and 10ºC. The high catecholase activity was observed using 4-methylcatechol as substrate at pH 6 and 30ºC and activation with sodium dodecyl sulphate (SDS) was unnecessary. The soluble PPO fraction was the most thermostable, as well as the most active, form of the enzyme. Fruits stored at 10ºC were undamaged, whereas those kept at 0 and 5ºC experienced chilling injury from days 6 and 8, respectively, as indicated by the decrease of lightness (L0) of pulp tissue. During exposure of fruits at 10ºC, the activities of soluble and insoluble PPO fractions increased, whereas, at 0ºC, PPO activity of both fractions decreased and maintained lower levels when browning of pulp tissue was observed. Soluble PPO activity at 0ºC was directly related to the value for L0.
Fil: Concellón, Analía. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Añon, Maria Cristina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Chaves, Alicia Raquel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina - Materia
-
EGGPLANT
SOLANUM MELONGENA L.
POLYPHENOL OXIDASE
CHILLING INJURY
STORAGE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/158769
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CONICET Digital (CONICET) |
spelling |
Characterization and changes in polyphenol oxidase from eggplant fruit (Solanum melongena L.) during storage at low temperatureConcellón, AnalíaAñon, Maria CristinaChaves, Alicia RaquelEGGPLANTSOLANUM MELONGENA L.POLYPHENOL OXIDASECHILLING INJURYSTORAGEhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Polyphenoloxidase (PPO) from eggplant fruit was characterized, and its catecholase activity studied during storage of fruit at 0 and 10ºC. The high catecholase activity was observed using 4-methylcatechol as substrate at pH 6 and 30ºC and activation with sodium dodecyl sulphate (SDS) was unnecessary. The soluble PPO fraction was the most thermostable, as well as the most active, form of the enzyme. Fruits stored at 10ºC were undamaged, whereas those kept at 0 and 5ºC experienced chilling injury from days 6 and 8, respectively, as indicated by the decrease of lightness (L0) of pulp tissue. During exposure of fruits at 10ºC, the activities of soluble and insoluble PPO fractions increased, whereas, at 0ºC, PPO activity of both fractions decreased and maintained lower levels when browning of pulp tissue was observed. Soluble PPO activity at 0ºC was directly related to the value for L0.Fil: Concellón, Analía. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Añon, Maria Cristina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Chaves, Alicia Raquel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaElsevier2004-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/158769Concellón, Analía; Añon, Maria Cristina; Chaves, Alicia Raquel; Characterization and changes in polyphenol oxidase from eggplant fruit (Solanum melongena L.) during storage at low temperature; Elsevier; Food Chemistry; 88; 1; 11-2004; 17-240308-81461873-7072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S030881460400055Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2004.01.017info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:52Zoai:ri.conicet.gov.ar:11336/158769instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:52.613CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Characterization and changes in polyphenol oxidase from eggplant fruit (Solanum melongena L.) during storage at low temperature |
title |
Characterization and changes in polyphenol oxidase from eggplant fruit (Solanum melongena L.) during storage at low temperature |
spellingShingle |
Characterization and changes in polyphenol oxidase from eggplant fruit (Solanum melongena L.) during storage at low temperature Concellón, Analía EGGPLANT SOLANUM MELONGENA L. POLYPHENOL OXIDASE CHILLING INJURY STORAGE |
title_short |
Characterization and changes in polyphenol oxidase from eggplant fruit (Solanum melongena L.) during storage at low temperature |
title_full |
Characterization and changes in polyphenol oxidase from eggplant fruit (Solanum melongena L.) during storage at low temperature |
title_fullStr |
Characterization and changes in polyphenol oxidase from eggplant fruit (Solanum melongena L.) during storage at low temperature |
title_full_unstemmed |
Characterization and changes in polyphenol oxidase from eggplant fruit (Solanum melongena L.) during storage at low temperature |
title_sort |
Characterization and changes in polyphenol oxidase from eggplant fruit (Solanum melongena L.) during storage at low temperature |
dc.creator.none.fl_str_mv |
Concellón, Analía Añon, Maria Cristina Chaves, Alicia Raquel |
author |
Concellón, Analía |
author_facet |
Concellón, Analía Añon, Maria Cristina Chaves, Alicia Raquel |
author_role |
author |
author2 |
Añon, Maria Cristina Chaves, Alicia Raquel |
author2_role |
author author |
dc.subject.none.fl_str_mv |
EGGPLANT SOLANUM MELONGENA L. POLYPHENOL OXIDASE CHILLING INJURY STORAGE |
topic |
EGGPLANT SOLANUM MELONGENA L. POLYPHENOL OXIDASE CHILLING INJURY STORAGE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Polyphenoloxidase (PPO) from eggplant fruit was characterized, and its catecholase activity studied during storage of fruit at 0 and 10ºC. The high catecholase activity was observed using 4-methylcatechol as substrate at pH 6 and 30ºC and activation with sodium dodecyl sulphate (SDS) was unnecessary. The soluble PPO fraction was the most thermostable, as well as the most active, form of the enzyme. Fruits stored at 10ºC were undamaged, whereas those kept at 0 and 5ºC experienced chilling injury from days 6 and 8, respectively, as indicated by the decrease of lightness (L0) of pulp tissue. During exposure of fruits at 10ºC, the activities of soluble and insoluble PPO fractions increased, whereas, at 0ºC, PPO activity of both fractions decreased and maintained lower levels when browning of pulp tissue was observed. Soluble PPO activity at 0ºC was directly related to the value for L0. Fil: Concellón, Analía. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina Fil: Añon, Maria Cristina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina Fil: Chaves, Alicia Raquel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina |
description |
Polyphenoloxidase (PPO) from eggplant fruit was characterized, and its catecholase activity studied during storage of fruit at 0 and 10ºC. The high catecholase activity was observed using 4-methylcatechol as substrate at pH 6 and 30ºC and activation with sodium dodecyl sulphate (SDS) was unnecessary. The soluble PPO fraction was the most thermostable, as well as the most active, form of the enzyme. Fruits stored at 10ºC were undamaged, whereas those kept at 0 and 5ºC experienced chilling injury from days 6 and 8, respectively, as indicated by the decrease of lightness (L0) of pulp tissue. During exposure of fruits at 10ºC, the activities of soluble and insoluble PPO fractions increased, whereas, at 0ºC, PPO activity of both fractions decreased and maintained lower levels when browning of pulp tissue was observed. Soluble PPO activity at 0ºC was directly related to the value for L0. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/158769 Concellón, Analía; Añon, Maria Cristina; Chaves, Alicia Raquel; Characterization and changes in polyphenol oxidase from eggplant fruit (Solanum melongena L.) during storage at low temperature; Elsevier; Food Chemistry; 88; 1; 11-2004; 17-24 0308-8146 1873-7072 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/158769 |
identifier_str_mv |
Concellón, Analía; Añon, Maria Cristina; Chaves, Alicia Raquel; Characterization and changes in polyphenol oxidase from eggplant fruit (Solanum melongena L.) during storage at low temperature; Elsevier; Food Chemistry; 88; 1; 11-2004; 17-24 0308-8146 1873-7072 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S030881460400055X info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2004.01.017 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842268821787770880 |
score |
13.13397 |