Echinococcus granulosus antigens activate different metabolic pathways in bone marrow-derived dendritic cells

Autores
Chop, Maia; Lausero, Luciano Nicolás; Franco, Micaela; Cumino, Andrea Carina; Rodríguez Rodrígues, Christian Fernando Ariel
Año de publicación
2024
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Background: Activation of bone marrow-derived dendritic cells (BMDCs) by inflammatory stimuliinduces a metabolic shift from oxidative phosphorylation (OXPHOS) to glycolysis, which is directlyrelated to the effectiveness of the immune response.Objectives: This study aimed to determine whether laminar layer (pLL) or hydatid fluid (HF) purifiedfrom Echinococcus granulosus can modify cellular metabolism.Methods: HF and pLL were collected from cysts. BMDCs were obtained from CF-1 mice and cultured incomplete RPMI medium. Mitochondrial metabolic state was evaluated by confocal microscopy andFACS using the probe JC-1. Oligomycin and 2-deoxy-D-glucose (2DG) were used as inhibitors.SEAHORSE and SCENITH assays were performed.Results: Morphological analysis of HF-stimulated BMDCs showed a decrease in mitochondria number,total area, perimeter, branches and junctions compared to the control. Cells stimulated with pLLexhibited similar behavior to LPS-treated cells, with a predominance of polarized mitochondria. In HFstimulated cells, a significant decrease in the high/low fluorescence ratio was observed by FACS, whichincreased upon 2DG treatment. No major changes were observed in pLL-stimulated cells (n = 3,**p<0,01, ****p<0,0001 HF or pLL vs. control). SEAHORSE assays revealed that the basal oxygenconsumption rate (OCR) was lower in HF-stimulated BMDCs than in the control. The extracellularacidification rate (ECAR) diminished with 2-DG treatment in all cases. A decrease in OCR was observedin pLL-stimulated BMDCs treated with oligomycin. SCENITH assays showed that cells stimulated withHF obtained their energy primarily from glycolysis, with minimal mitochondrial dependence. In contrast,pLL-stimulated BMDCs showed greater amino acid and fatty acid oxidation capacities (n = 3, ***p<0,001HF or pLL vs. control).Conclusion: E. granulosus antigen recognition induces metabolic reprogramming in BMDCs thatdirectly impact in their cell function and immune response against the parasite.
Fil: Chop, Maia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Lausero, Luciano Nicolás. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Franco, Micaela. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Cumino, Andrea Carina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Rodríguez Rodrígues, Christian Fernando Ariel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
XXVII Congreso de la Federación Latinoamericana de Parasitología y XII Congreso de la Sociedad Argentina de Protozoología
Buenos Aires
Argentina
Sociedad Argentina de Protozoología
Materia
Dendritic cells
metabolic reprogramation
echinococcus antigens
TOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/277770
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/277770
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Echinococcus granulosus antigens activate different metabolic pathways in bone marrow-derived dendritic cellsChop, MaiaLausero, Luciano NicolásFranco, MicaelaCumino, Andrea CarinaRodríguez Rodrígues, Christian Fernando ArielDendritic cellsmetabolic reprogramationechinococcus antigensTORhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: Activation of bone marrow-derived dendritic cells (BMDCs) by inflammatory stimuliinduces a metabolic shift from oxidative phosphorylation (OXPHOS) to glycolysis, which is directlyrelated to the effectiveness of the immune response.Objectives: This study aimed to determine whether laminar layer (pLL) or hydatid fluid (HF) purifiedfrom Echinococcus granulosus can modify cellular metabolism.Methods: HF and pLL were collected from cysts. BMDCs were obtained from CF-1 mice and cultured incomplete RPMI medium. Mitochondrial metabolic state was evaluated by confocal microscopy andFACS using the probe JC-1. Oligomycin and 2-deoxy-D-glucose (2DG) were used as inhibitors.SEAHORSE and SCENITH assays were performed.Results: Morphological analysis of HF-stimulated BMDCs showed a decrease in mitochondria number,total area, perimeter, branches and junctions compared to the control. Cells stimulated with pLLexhibited similar behavior to LPS-treated cells, with a predominance of polarized mitochondria. In HFstimulated cells, a significant decrease in the high/low fluorescence ratio was observed by FACS, whichincreased upon 2DG treatment. No major changes were observed in pLL-stimulated cells (n = 3,**p<0,01, ****p<0,0001 HF or pLL vs. control). SEAHORSE assays revealed that the basal oxygenconsumption rate (OCR) was lower in HF-stimulated BMDCs than in the control. The extracellularacidification rate (ECAR) diminished with 2-DG treatment in all cases. A decrease in OCR was observedin pLL-stimulated BMDCs treated with oligomycin. SCENITH assays showed that cells stimulated withHF obtained their energy primarily from glycolysis, with minimal mitochondrial dependence. In contrast,pLL-stimulated BMDCs showed greater amino acid and fatty acid oxidation capacities (n = 3, ***p<0,001HF or pLL vs. control).Conclusion: E. granulosus antigen recognition induces metabolic reprogramming in BMDCs thatdirectly impact in their cell function and immune response against the parasite.Fil: Chop, Maia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Lausero, Luciano Nicolás. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Franco, Micaela. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Cumino, Andrea Carina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Rodríguez Rodrígues, Christian Fernando Ariel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaXXVII Congreso de la Federación Latinoamericana de Parasitología y XII Congreso de la Sociedad Argentina de ProtozoologíaBuenos AiresArgentinaSociedad Argentina de ProtozoologíaSociedad Argentina de Protozoología2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/277770Echinococcus granulosus antigens activate different metabolic pathways in bone marrow-derived dendritic cells; XXVII Congreso de la Federación Latinoamericana de Parasitología y XII Congreso de la Sociedad Argentina de Protozoología; Buenos Aires; Argentina; 2024; 1-52953-5751CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://protozoologia.org.ar/revista-parasitus/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T13:29:30Zoai:ri.conicet.gov.ar:11336/277770instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 13:29:30.929CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Echinococcus granulosus antigens activate different metabolic pathways in bone marrow-derived dendritic cells
title Echinococcus granulosus antigens activate different metabolic pathways in bone marrow-derived dendritic cells
spellingShingle Echinococcus granulosus antigens activate different metabolic pathways in bone marrow-derived dendritic cells
Chop, Maia
Dendritic cells
metabolic reprogramation
echinococcus antigens
TOR
title_short Echinococcus granulosus antigens activate different metabolic pathways in bone marrow-derived dendritic cells
title_full Echinococcus granulosus antigens activate different metabolic pathways in bone marrow-derived dendritic cells
title_fullStr Echinococcus granulosus antigens activate different metabolic pathways in bone marrow-derived dendritic cells
title_full_unstemmed Echinococcus granulosus antigens activate different metabolic pathways in bone marrow-derived dendritic cells
title_sort Echinococcus granulosus antigens activate different metabolic pathways in bone marrow-derived dendritic cells
dc.creator.none.fl_str_mv Chop, Maia
Lausero, Luciano Nicolás
Franco, Micaela
Cumino, Andrea Carina
Rodríguez Rodrígues, Christian Fernando Ariel
author Chop, Maia
author_facet Chop, Maia
Lausero, Luciano Nicolás
Franco, Micaela
Cumino, Andrea Carina
Rodríguez Rodrígues, Christian Fernando Ariel
author_role author
author2 Lausero, Luciano Nicolás
Franco, Micaela
Cumino, Andrea Carina
Rodríguez Rodrígues, Christian Fernando Ariel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Dendritic cells
metabolic reprogramation
echinococcus antigens
TOR
topic Dendritic cells
metabolic reprogramation
echinococcus antigens
TOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Activation of bone marrow-derived dendritic cells (BMDCs) by inflammatory stimuliinduces a metabolic shift from oxidative phosphorylation (OXPHOS) to glycolysis, which is directlyrelated to the effectiveness of the immune response.Objectives: This study aimed to determine whether laminar layer (pLL) or hydatid fluid (HF) purifiedfrom Echinococcus granulosus can modify cellular metabolism.Methods: HF and pLL were collected from cysts. BMDCs were obtained from CF-1 mice and cultured incomplete RPMI medium. Mitochondrial metabolic state was evaluated by confocal microscopy andFACS using the probe JC-1. Oligomycin and 2-deoxy-D-glucose (2DG) were used as inhibitors.SEAHORSE and SCENITH assays were performed.Results: Morphological analysis of HF-stimulated BMDCs showed a decrease in mitochondria number,total area, perimeter, branches and junctions compared to the control. Cells stimulated with pLLexhibited similar behavior to LPS-treated cells, with a predominance of polarized mitochondria. In HFstimulated cells, a significant decrease in the high/low fluorescence ratio was observed by FACS, whichincreased upon 2DG treatment. No major changes were observed in pLL-stimulated cells (n = 3,**p<0,01, ****p<0,0001 HF or pLL vs. control). SEAHORSE assays revealed that the basal oxygenconsumption rate (OCR) was lower in HF-stimulated BMDCs than in the control. The extracellularacidification rate (ECAR) diminished with 2-DG treatment in all cases. A decrease in OCR was observedin pLL-stimulated BMDCs treated with oligomycin. SCENITH assays showed that cells stimulated withHF obtained their energy primarily from glycolysis, with minimal mitochondrial dependence. In contrast,pLL-stimulated BMDCs showed greater amino acid and fatty acid oxidation capacities (n = 3, ***p<0,001HF or pLL vs. control).Conclusion: E. granulosus antigen recognition induces metabolic reprogramming in BMDCs thatdirectly impact in their cell function and immune response against the parasite.
Fil: Chop, Maia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Lausero, Luciano Nicolás. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Franco, Micaela. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Cumino, Andrea Carina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Rodríguez Rodrígues, Christian Fernando Ariel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
XXVII Congreso de la Federación Latinoamericana de Parasitología y XII Congreso de la Sociedad Argentina de Protozoología
Buenos Aires
Argentina
Sociedad Argentina de Protozoología
description Background: Activation of bone marrow-derived dendritic cells (BMDCs) by inflammatory stimuliinduces a metabolic shift from oxidative phosphorylation (OXPHOS) to glycolysis, which is directlyrelated to the effectiveness of the immune response.Objectives: This study aimed to determine whether laminar layer (pLL) or hydatid fluid (HF) purifiedfrom Echinococcus granulosus can modify cellular metabolism.Methods: HF and pLL were collected from cysts. BMDCs were obtained from CF-1 mice and cultured incomplete RPMI medium. Mitochondrial metabolic state was evaluated by confocal microscopy andFACS using the probe JC-1. Oligomycin and 2-deoxy-D-glucose (2DG) were used as inhibitors.SEAHORSE and SCENITH assays were performed.Results: Morphological analysis of HF-stimulated BMDCs showed a decrease in mitochondria number,total area, perimeter, branches and junctions compared to the control. Cells stimulated with pLLexhibited similar behavior to LPS-treated cells, with a predominance of polarized mitochondria. In HFstimulated cells, a significant decrease in the high/low fluorescence ratio was observed by FACS, whichincreased upon 2DG treatment. No major changes were observed in pLL-stimulated cells (n = 3,**p<0,01, ****p<0,0001 HF or pLL vs. control). SEAHORSE assays revealed that the basal oxygenconsumption rate (OCR) was lower in HF-stimulated BMDCs than in the control. The extracellularacidification rate (ECAR) diminished with 2-DG treatment in all cases. A decrease in OCR was observedin pLL-stimulated BMDCs treated with oligomycin. SCENITH assays showed that cells stimulated withHF obtained their energy primarily from glycolysis, with minimal mitochondrial dependence. In contrast,pLL-stimulated BMDCs showed greater amino acid and fatty acid oxidation capacities (n = 3, ***p<0,001HF or pLL vs. control).Conclusion: E. granulosus antigen recognition induces metabolic reprogramming in BMDCs thatdirectly impact in their cell function and immune response against the parasite.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/277770
Echinococcus granulosus antigens activate different metabolic pathways in bone marrow-derived dendritic cells; XXVII Congreso de la Federación Latinoamericana de Parasitología y XII Congreso de la Sociedad Argentina de Protozoología; Buenos Aires; Argentina; 2024; 1-5
2953-5751
CONICET Digital
CONICET
url http://hdl.handle.net/11336/277770
identifier_str_mv Echinococcus granulosus antigens activate different metabolic pathways in bone marrow-derived dendritic cells; XXVII Congreso de la Federación Latinoamericana de Parasitología y XII Congreso de la Sociedad Argentina de Protozoología; Buenos Aires; Argentina; 2024; 1-5
2953-5751
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://protozoologia.org.ar/revista-parasitus/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Protozoología
publisher.none.fl_str_mv Sociedad Argentina de Protozoología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.952241

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