Hydatid fluid from Echinococcus granulosus induce the autophagy process in dendritic cells and promote antigen presentation and T- cell proliferation

Autores
Chop, Maia; Plá, Natalia; Loos, Julia Alexandra; Nicolao, María Celeste; Cumino, Andrea Carina; Rodríguez Rodrígues, Christian Fernando Ariel
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Background: Autophagy is an important process for the presentation of endogenous and exogenous proteins on MHC I and II molecules, promoting activation of CD8+ and CD4+ T cells respectively. The aim of this work is to analyze if hydatid fluid (HF) from Echinococcus, constituted by a wide range of parasite proteins could trigger autophagy improving antigen presentation and T cell proliferation. Methods: BMDCs were cultured in complete RPMI. Hydatid fluid (HF) was punctured from the hydatid cysts collected of infected cattle slaughtered. Antigen uptake was measured with (FITC-OVA) in BMDCs using a standard method. HF-stimulated BMDCs, were evaluated in autophagy induction and MHC II expression. For it, fixed cells were immunostained with LC3-clone H50 and analyzed them by immunofluorescence confocal microscopy. CFSE-stained splenocytes were co-incubated with BMDCs using a DC: splenocyte ratio of 1:4 Cellular proliferation was assayed after 4 days of culture by flow cytometry. Results: First, we evaluated if stimulation of HF during 18 h in BMDCs, induce different rates of antigen uptake. Effectively, the presence of Echinococcus antigens induces a markedly decreased OVA-uptake compared to control (**p <0.01, n=3). Next, we studied if stimulation with Eg antigens induces changes in the basal level of autophagy. HF-stimulated BMDCs significantly enhanced the mean fluorescence intensity of MHC II and LC3 and showed a trend in the increment of number and the average size of LC3-positive structures in comparison with unstimulated cells (*p<0.05, **p<0.01, ***p<0.001 HF-stimulated cells vs controls). Finally, we observed that culture splenocytes in the presence of stimulated DC induce their proliferation % CFSE+ cells CTRL:99%, HF:55% (***p<0.001, n=3). Conclusions: These data suggest that HF of Echinococcus induces an increase in autophagy processes promoting the presentation of exogenous antigens presented in MHC II molecules to improve T cell proliferation.
Fil: Chop, Maia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Plá, Natalia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Loos, Julia Alexandra. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Nicolao, María Celeste. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Cumino, Andrea Carina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Rodríguez Rodrígues, Christian Fernando Ariel. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Materia
Echinococcus granulosus
dendritic cells
hydatic fluid
autophagy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/154553
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/154553
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Hydatid fluid from Echinococcus granulosus induce the autophagy process in dendritic cells and promote antigen presentation and T- cell proliferationChop, MaiaPlá, NataliaLoos, Julia AlexandraNicolao, María CelesteCumino, Andrea CarinaRodríguez Rodrígues, Christian Fernando ArielEchinococcus granulosusdendritic cellshydatic fluidautophagyhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: Autophagy is an important process for the presentation of endogenous and exogenous proteins on MHC I and II molecules, promoting activation of CD8+ and CD4+ T cells respectively. The aim of this work is to analyze if hydatid fluid (HF) from Echinococcus, constituted by a wide range of parasite proteins could trigger autophagy improving antigen presentation and T cell proliferation. Methods: BMDCs were cultured in complete RPMI. Hydatid fluid (HF) was punctured from the hydatid cysts collected of infected cattle slaughtered. Antigen uptake was measured with (FITC-OVA) in BMDCs using a standard method. HF-stimulated BMDCs, were evaluated in autophagy induction and MHC II expression. For it, fixed cells were immunostained with LC3-clone H50 and analyzed them by immunofluorescence confocal microscopy. CFSE-stained splenocytes were co-incubated with BMDCs using a DC: splenocyte ratio of 1:4 Cellular proliferation was assayed after 4 days of culture by flow cytometry. Results: First, we evaluated if stimulation of HF during 18 h in BMDCs, induce different rates of antigen uptake. Effectively, the presence of Echinococcus antigens induces a markedly decreased OVA-uptake compared to control (**p <0.01, n=3). Next, we studied if stimulation with Eg antigens induces changes in the basal level of autophagy. HF-stimulated BMDCs significantly enhanced the mean fluorescence intensity of MHC II and LC3 and showed a trend in the increment of number and the average size of LC3-positive structures in comparison with unstimulated cells (*p<0.05, **p<0.01, ***p<0.001 HF-stimulated cells vs controls). Finally, we observed that culture splenocytes in the presence of stimulated DC induce their proliferation % CFSE+ cells CTRL:99%, HF:55% (***p<0.001, n=3). Conclusions: These data suggest that HF of Echinococcus induces an increase in autophagy processes promoting the presentation of exogenous antigens presented in MHC II molecules to improve T cell proliferation.Fil: Chop, Maia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Plá, Natalia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; ArgentinaFil: Loos, Julia Alexandra. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Nicolao, María Celeste. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Cumino, Andrea Carina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Rodríguez Rodrígues, Christian Fernando Ariel. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaLXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de FisiologíaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista Medicina2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/154553Hydatid fluid from Echinococcus granulosus induce the autophagy process in dendritic cells and promote antigen presentation and T- cell proliferation; LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2020; 153-1530025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://inmunologia.org.ar/documentos/pdf/revista_medicina_2020.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:59Zoai:ri.conicet.gov.ar:11336/154553instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:00.069CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hydatid fluid from Echinococcus granulosus induce the autophagy process in dendritic cells and promote antigen presentation and T- cell proliferation
title Hydatid fluid from Echinococcus granulosus induce the autophagy process in dendritic cells and promote antigen presentation and T- cell proliferation
spellingShingle Hydatid fluid from Echinococcus granulosus induce the autophagy process in dendritic cells and promote antigen presentation and T- cell proliferation
Chop, Maia
Echinococcus granulosus
dendritic cells
hydatic fluid
autophagy
title_short Hydatid fluid from Echinococcus granulosus induce the autophagy process in dendritic cells and promote antigen presentation and T- cell proliferation
title_full Hydatid fluid from Echinococcus granulosus induce the autophagy process in dendritic cells and promote antigen presentation and T- cell proliferation
title_fullStr Hydatid fluid from Echinococcus granulosus induce the autophagy process in dendritic cells and promote antigen presentation and T- cell proliferation
title_full_unstemmed Hydatid fluid from Echinococcus granulosus induce the autophagy process in dendritic cells and promote antigen presentation and T- cell proliferation
title_sort Hydatid fluid from Echinococcus granulosus induce the autophagy process in dendritic cells and promote antigen presentation and T- cell proliferation
dc.creator.none.fl_str_mv Chop, Maia
Plá, Natalia
Loos, Julia Alexandra
Nicolao, María Celeste
Cumino, Andrea Carina
Rodríguez Rodrígues, Christian Fernando Ariel
author Chop, Maia
author_facet Chop, Maia
Plá, Natalia
Loos, Julia Alexandra
Nicolao, María Celeste
Cumino, Andrea Carina
Rodríguez Rodrígues, Christian Fernando Ariel
author_role author
author2 Plá, Natalia
Loos, Julia Alexandra
Nicolao, María Celeste
Cumino, Andrea Carina
Rodríguez Rodrígues, Christian Fernando Ariel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Echinococcus granulosus
dendritic cells
hydatic fluid
autophagy
topic Echinococcus granulosus
dendritic cells
hydatic fluid
autophagy
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Autophagy is an important process for the presentation of endogenous and exogenous proteins on MHC I and II molecules, promoting activation of CD8+ and CD4+ T cells respectively. The aim of this work is to analyze if hydatid fluid (HF) from Echinococcus, constituted by a wide range of parasite proteins could trigger autophagy improving antigen presentation and T cell proliferation. Methods: BMDCs were cultured in complete RPMI. Hydatid fluid (HF) was punctured from the hydatid cysts collected of infected cattle slaughtered. Antigen uptake was measured with (FITC-OVA) in BMDCs using a standard method. HF-stimulated BMDCs, were evaluated in autophagy induction and MHC II expression. For it, fixed cells were immunostained with LC3-clone H50 and analyzed them by immunofluorescence confocal microscopy. CFSE-stained splenocytes were co-incubated with BMDCs using a DC: splenocyte ratio of 1:4 Cellular proliferation was assayed after 4 days of culture by flow cytometry. Results: First, we evaluated if stimulation of HF during 18 h in BMDCs, induce different rates of antigen uptake. Effectively, the presence of Echinococcus antigens induces a markedly decreased OVA-uptake compared to control (**p <0.01, n=3). Next, we studied if stimulation with Eg antigens induces changes in the basal level of autophagy. HF-stimulated BMDCs significantly enhanced the mean fluorescence intensity of MHC II and LC3 and showed a trend in the increment of number and the average size of LC3-positive structures in comparison with unstimulated cells (*p<0.05, **p<0.01, ***p<0.001 HF-stimulated cells vs controls). Finally, we observed that culture splenocytes in the presence of stimulated DC induce their proliferation % CFSE+ cells CTRL:99%, HF:55% (***p<0.001, n=3). Conclusions: These data suggest that HF of Echinococcus induces an increase in autophagy processes promoting the presentation of exogenous antigens presented in MHC II molecules to improve T cell proliferation.
Fil: Chop, Maia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Plá, Natalia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
Fil: Loos, Julia Alexandra. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Nicolao, María Celeste. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Cumino, Andrea Carina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Rodríguez Rodrígues, Christian Fernando Ariel. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
description Background: Autophagy is an important process for the presentation of endogenous and exogenous proteins on MHC I and II molecules, promoting activation of CD8+ and CD4+ T cells respectively. The aim of this work is to analyze if hydatid fluid (HF) from Echinococcus, constituted by a wide range of parasite proteins could trigger autophagy improving antigen presentation and T cell proliferation. Methods: BMDCs were cultured in complete RPMI. Hydatid fluid (HF) was punctured from the hydatid cysts collected of infected cattle slaughtered. Antigen uptake was measured with (FITC-OVA) in BMDCs using a standard method. HF-stimulated BMDCs, were evaluated in autophagy induction and MHC II expression. For it, fixed cells were immunostained with LC3-clone H50 and analyzed them by immunofluorescence confocal microscopy. CFSE-stained splenocytes were co-incubated with BMDCs using a DC: splenocyte ratio of 1:4 Cellular proliferation was assayed after 4 days of culture by flow cytometry. Results: First, we evaluated if stimulation of HF during 18 h in BMDCs, induce different rates of antigen uptake. Effectively, the presence of Echinococcus antigens induces a markedly decreased OVA-uptake compared to control (**p <0.01, n=3). Next, we studied if stimulation with Eg antigens induces changes in the basal level of autophagy. HF-stimulated BMDCs significantly enhanced the mean fluorescence intensity of MHC II and LC3 and showed a trend in the increment of number and the average size of LC3-positive structures in comparison with unstimulated cells (*p<0.05, **p<0.01, ***p<0.001 HF-stimulated cells vs controls). Finally, we observed that culture splenocytes in the presence of stimulated DC induce their proliferation % CFSE+ cells CTRL:99%, HF:55% (***p<0.001, n=3). Conclusions: These data suggest that HF of Echinococcus induces an increase in autophagy processes promoting the presentation of exogenous antigens presented in MHC II molecules to improve T cell proliferation.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/154553
Hydatid fluid from Echinococcus granulosus induce the autophagy process in dendritic cells and promote antigen presentation and T- cell proliferation; LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2020; 153-153
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/154553
identifier_str_mv Hydatid fluid from Echinococcus granulosus induce the autophagy process in dendritic cells and promote antigen presentation and T- cell proliferation; LXV Reunión anual de la Sociedad Argentina de Investigación Clínica; LXVII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2020; 153-153
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://inmunologia.org.ar/documentos/pdf/revista_medicina_2020.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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