Enterococcus faecalis Virulence Regulator FsrA Binding to Target Promoters

Autores
del Papa, Maria Florencia; Perego, Marta
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The FsrABDC signal transduction system is a major virulence regulator in Enterococcus faecalis. The FsrC sensor histidine kinase, upon activation by the gelatinase biosynthesis-activating pheromone (GBAP) peptide encoded by the fsrBD genes, phosphorylates the FsrA response regulator required for the transcription of the fsrBDC and the gelE-sprE genes from the fsrB promoter and the gelE promoter, respectively. FsrA belongs to the LytTR family of proteins, which includes other virulence regulators, such as AgrA of Staphylococcus aureus, AlgR of Pseudomonas aeruginosa, and VirR of Clostridium perfringens. The LytTR DNA-binding domain that characterizes these proteins generally binds to two imperfect direct repeats separated by a number of bases that place the repeats on the same face of the DNA helix. In this study, we demonstrated that FsrA also binds to two imperfect direct repeats separated by 13 bp, based on the consensus sequence of FsrA, T/AT/CAA/GG GAA/G, which is consistent with the binding characteristics of LytTR domains.
Fil: del Papa, Maria Florencia. The Scripps Research Institute; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perego, Marta. The Scripps Research Institute; Estados Unidos
Materia
ENTEROCOCCUS FAECALIS
TWO COMPONENT SYSTEM
FSR A
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/271398
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Enterococcus faecalis Virulence Regulator FsrA Binding to Target Promotersdel Papa, Maria FlorenciaPerego, MartaENTEROCOCCUS FAECALISTWO COMPONENT SYSTEMFSR Ahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The FsrABDC signal transduction system is a major virulence regulator in Enterococcus faecalis. The FsrC sensor histidine kinase, upon activation by the gelatinase biosynthesis-activating pheromone (GBAP) peptide encoded by the fsrBD genes, phosphorylates the FsrA response regulator required for the transcription of the fsrBDC and the gelE-sprE genes from the fsrB promoter and the gelE promoter, respectively. FsrA belongs to the LytTR family of proteins, which includes other virulence regulators, such as AgrA of Staphylococcus aureus, AlgR of Pseudomonas aeruginosa, and VirR of Clostridium perfringens. The LytTR DNA-binding domain that characterizes these proteins generally binds to two imperfect direct repeats separated by a number of bases that place the repeats on the same face of the DNA helix. In this study, we demonstrated that FsrA also binds to two imperfect direct repeats separated by 13 bp, based on the consensus sequence of FsrA, T/AT/CAA/GG GAA/G, which is consistent with the binding characteristics of LytTR domains.Fil: del Papa, Maria Florencia. The Scripps Research Institute; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perego, Marta. The Scripps Research Institute; Estados UnidosAmerican Society for Microbiology2011-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/271398del Papa, Maria Florencia; Perego, Marta; Enterococcus faecalis Virulence Regulator FsrA Binding to Target Promoters; American Society for Microbiology; Journal of Bacteriology; 193; 7; 1-2011; 1527-15320021-9193CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1128/JB.01522-10info:eu-repo/semantics/altIdentifier/url/https://pmc.ncbi.nlm.nih.gov/articles/PMC3067650/info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/jb.01522-10info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:46Zoai:ri.conicet.gov.ar:11336/271398instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:46.726CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enterococcus faecalis Virulence Regulator FsrA Binding to Target Promoters
title Enterococcus faecalis Virulence Regulator FsrA Binding to Target Promoters
spellingShingle Enterococcus faecalis Virulence Regulator FsrA Binding to Target Promoters
del Papa, Maria Florencia
ENTEROCOCCUS FAECALIS
TWO COMPONENT SYSTEM
FSR A
title_short Enterococcus faecalis Virulence Regulator FsrA Binding to Target Promoters
title_full Enterococcus faecalis Virulence Regulator FsrA Binding to Target Promoters
title_fullStr Enterococcus faecalis Virulence Regulator FsrA Binding to Target Promoters
title_full_unstemmed Enterococcus faecalis Virulence Regulator FsrA Binding to Target Promoters
title_sort Enterococcus faecalis Virulence Regulator FsrA Binding to Target Promoters
dc.creator.none.fl_str_mv del Papa, Maria Florencia
Perego, Marta
author del Papa, Maria Florencia
author_facet del Papa, Maria Florencia
Perego, Marta
author_role author
author2 Perego, Marta
author2_role author
dc.subject.none.fl_str_mv ENTEROCOCCUS FAECALIS
TWO COMPONENT SYSTEM
FSR A
topic ENTEROCOCCUS FAECALIS
TWO COMPONENT SYSTEM
FSR A
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The FsrABDC signal transduction system is a major virulence regulator in Enterococcus faecalis. The FsrC sensor histidine kinase, upon activation by the gelatinase biosynthesis-activating pheromone (GBAP) peptide encoded by the fsrBD genes, phosphorylates the FsrA response regulator required for the transcription of the fsrBDC and the gelE-sprE genes from the fsrB promoter and the gelE promoter, respectively. FsrA belongs to the LytTR family of proteins, which includes other virulence regulators, such as AgrA of Staphylococcus aureus, AlgR of Pseudomonas aeruginosa, and VirR of Clostridium perfringens. The LytTR DNA-binding domain that characterizes these proteins generally binds to two imperfect direct repeats separated by a number of bases that place the repeats on the same face of the DNA helix. In this study, we demonstrated that FsrA also binds to two imperfect direct repeats separated by 13 bp, based on the consensus sequence of FsrA, T/AT/CAA/GG GAA/G, which is consistent with the binding characteristics of LytTR domains.
Fil: del Papa, Maria Florencia. The Scripps Research Institute; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perego, Marta. The Scripps Research Institute; Estados Unidos
description The FsrABDC signal transduction system is a major virulence regulator in Enterococcus faecalis. The FsrC sensor histidine kinase, upon activation by the gelatinase biosynthesis-activating pheromone (GBAP) peptide encoded by the fsrBD genes, phosphorylates the FsrA response regulator required for the transcription of the fsrBDC and the gelE-sprE genes from the fsrB promoter and the gelE promoter, respectively. FsrA belongs to the LytTR family of proteins, which includes other virulence regulators, such as AgrA of Staphylococcus aureus, AlgR of Pseudomonas aeruginosa, and VirR of Clostridium perfringens. The LytTR DNA-binding domain that characterizes these proteins generally binds to two imperfect direct repeats separated by a number of bases that place the repeats on the same face of the DNA helix. In this study, we demonstrated that FsrA also binds to two imperfect direct repeats separated by 13 bp, based on the consensus sequence of FsrA, T/AT/CAA/GG GAA/G, which is consistent with the binding characteristics of LytTR domains.
publishDate 2011
dc.date.none.fl_str_mv 2011-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/271398
del Papa, Maria Florencia; Perego, Marta; Enterococcus faecalis Virulence Regulator FsrA Binding to Target Promoters; American Society for Microbiology; Journal of Bacteriology; 193; 7; 1-2011; 1527-1532
0021-9193
CONICET Digital
CONICET
url http://hdl.handle.net/11336/271398
identifier_str_mv del Papa, Maria Florencia; Perego, Marta; Enterococcus faecalis Virulence Regulator FsrA Binding to Target Promoters; American Society for Microbiology; Journal of Bacteriology; 193; 7; 1-2011; 1527-1532
0021-9193
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1128/JB.01522-10
info:eu-repo/semantics/altIdentifier/url/https://pmc.ncbi.nlm.nih.gov/articles/PMC3067650/
info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/jb.01522-10
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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