Anthocyanins as inhibitors of α-glucosidase and pancreatic lipase activity

Autores
Mosse, Juana Inés; Galleano, Mónica Liliana; Fraga, César Guillermo
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Food components providing glucose and fat-lowering effects in the body can be useful to attenuate the negative health effects associated to high-carbohydrates and/or high-fat diets. Here, anthocyanins were studied as potential inhibitors of α-glucosidase (α-G) and pancreatic lipase (PL) activities, two key enzymes responsible for carbohydrates and fats digestion. The anthocyanin profile of extracts obtained from bilberry (BB), blackcurrant (BC) and black rice (BR) was characterized. The capacity of extracts, pure anthocyanins, and mixtures (representative of anthocyanin extracts composition) to inhibit the activity of α-G and PL was evaluated using enzymatic assays, including acarbose and orlistat as positive controls for α-G and PL, respectively. In a concentration range of 20-320 μg/ mL, extracts showed a dose-dependent inhibitory effects on α-G activity. The calculated IC50 were 87 ± 7, 76 ± 7 and 236 ± 25 μg/mL for BB, BC and BR, respectively. Some pure anthocyanins showed inhibitory actions (cyanidin-3-O-glucoside˃delphinidin-3-O-glucoside˃petunidin-3-O-glucoside) whereas the mixtures were ineffective at the assayed range concentration. In a broader concentration range (0-2000 μg/mL), extracts did not show significant inhibition on porcine PL activity, but most of the pure anthocyanins were effective (petunidin-3-O-glucoside˃cyanidin-3-O-glucoside˃delphinidin-3-O-glucoside˃malvidin-3-O-glucoside). No inhibition was observed for peonidin-3-O-glucoside and mixtures of compounds. In summary, anthocyanins would contribute only marginally to the in vitro α-G inhibition. Regarding PL activity, their inhibitory capacity was reduced when present in extracts or mixtures, suggesting complex interactions with other components under the in vitro used conditions. In vivo studies are needed to confirm the effects of extracts/ pure anthocyanins on enzymatic activities observed here.
Fil: Mosse, Juana Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analitica y Fisicoquímica. Cátedra de Fisicoquímica; Argentina
Fil: Galleano, Mónica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analitica y Fisicoquímica. Cátedra de Fisicoquímica; Argentina
Fil: Fraga, César Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analitica y Fisicoquímica. Cátedra de Fisicoquímica; Argentina
LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Asociación Argentina de Fisiología
Ciudad Autonoma de Buenos Aires
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Asociación Argentina de Fisiología
Materia
OBESITY
ANTIOXIDANT
FLAVONOIDS
ENZYMES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199438
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/199438
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Anthocyanins as inhibitors of α-glucosidase and pancreatic lipase activityMosse, Juana InésGalleano, Mónica LilianaFraga, César GuillermoOBESITYANTIOXIDANTFLAVONOIDSENZYMEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Food components providing glucose and fat-lowering effects in the body can be useful to attenuate the negative health effects associated to high-carbohydrates and/or high-fat diets. Here, anthocyanins were studied as potential inhibitors of α-glucosidase (α-G) and pancreatic lipase (PL) activities, two key enzymes responsible for carbohydrates and fats digestion. The anthocyanin profile of extracts obtained from bilberry (BB), blackcurrant (BC) and black rice (BR) was characterized. The capacity of extracts, pure anthocyanins, and mixtures (representative of anthocyanin extracts composition) to inhibit the activity of α-G and PL was evaluated using enzymatic assays, including acarbose and orlistat as positive controls for α-G and PL, respectively. In a concentration range of 20-320 μg/ mL, extracts showed a dose-dependent inhibitory effects on α-G activity. The calculated IC50 were 87 ± 7, 76 ± 7 and 236 ± 25 μg/mL for BB, BC and BR, respectively. Some pure anthocyanins showed inhibitory actions (cyanidin-3-O-glucoside˃delphinidin-3-O-glucoside˃petunidin-3-O-glucoside) whereas the mixtures were ineffective at the assayed range concentration. In a broader concentration range (0-2000 μg/mL), extracts did not show significant inhibition on porcine PL activity, but most of the pure anthocyanins were effective (petunidin-3-O-glucoside˃cyanidin-3-O-glucoside˃delphinidin-3-O-glucoside˃malvidin-3-O-glucoside). No inhibition was observed for peonidin-3-O-glucoside and mixtures of compounds. In summary, anthocyanins would contribute only marginally to the in vitro α-G inhibition. Regarding PL activity, their inhibitory capacity was reduced when present in extracts or mixtures, suggesting complex interactions with other components under the in vitro used conditions. In vivo studies are needed to confirm the effects of extracts/ pure anthocyanins on enzymatic activities observed here.Fil: Mosse, Juana Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analitica y Fisicoquímica. Cátedra de Fisicoquímica; ArgentinaFil: Galleano, Mónica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analitica y Fisicoquímica. Cátedra de Fisicoquímica; ArgentinaFil: Fraga, César Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analitica y Fisicoquímica. Cátedra de Fisicoquímica; ArgentinaLXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Asociación Argentina de FisiologíaCiudad Autonoma de Buenos AiresArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaAsociación Argentina de FisiologíaFundacion Revista Medicina2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199438Anthocyanins as inhibitors of α-glucosidase and pancreatic lipase activity; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Asociación Argentina de Fisiología; Ciudad Autonoma de Buenos Aires; Argentina; 2020; 1-30025-7680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reunion-anualNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:57:30Zoai:ri.conicet.gov.ar:11336/199438instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:57:30.89CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anthocyanins as inhibitors of α-glucosidase and pancreatic lipase activity
title Anthocyanins as inhibitors of α-glucosidase and pancreatic lipase activity
spellingShingle Anthocyanins as inhibitors of α-glucosidase and pancreatic lipase activity
Mosse, Juana Inés
OBESITY
ANTIOXIDANT
FLAVONOIDS
ENZYMES
title_short Anthocyanins as inhibitors of α-glucosidase and pancreatic lipase activity
title_full Anthocyanins as inhibitors of α-glucosidase and pancreatic lipase activity
title_fullStr Anthocyanins as inhibitors of α-glucosidase and pancreatic lipase activity
title_full_unstemmed Anthocyanins as inhibitors of α-glucosidase and pancreatic lipase activity
title_sort Anthocyanins as inhibitors of α-glucosidase and pancreatic lipase activity
dc.creator.none.fl_str_mv Mosse, Juana Inés
Galleano, Mónica Liliana
Fraga, César Guillermo
author Mosse, Juana Inés
author_facet Mosse, Juana Inés
Galleano, Mónica Liliana
Fraga, César Guillermo
author_role author
author2 Galleano, Mónica Liliana
Fraga, César Guillermo
author2_role author
author
dc.subject.none.fl_str_mv OBESITY
ANTIOXIDANT
FLAVONOIDS
ENZYMES
topic OBESITY
ANTIOXIDANT
FLAVONOIDS
ENZYMES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Food components providing glucose and fat-lowering effects in the body can be useful to attenuate the negative health effects associated to high-carbohydrates and/or high-fat diets. Here, anthocyanins were studied as potential inhibitors of α-glucosidase (α-G) and pancreatic lipase (PL) activities, two key enzymes responsible for carbohydrates and fats digestion. The anthocyanin profile of extracts obtained from bilberry (BB), blackcurrant (BC) and black rice (BR) was characterized. The capacity of extracts, pure anthocyanins, and mixtures (representative of anthocyanin extracts composition) to inhibit the activity of α-G and PL was evaluated using enzymatic assays, including acarbose and orlistat as positive controls for α-G and PL, respectively. In a concentration range of 20-320 μg/ mL, extracts showed a dose-dependent inhibitory effects on α-G activity. The calculated IC50 were 87 ± 7, 76 ± 7 and 236 ± 25 μg/mL for BB, BC and BR, respectively. Some pure anthocyanins showed inhibitory actions (cyanidin-3-O-glucoside˃delphinidin-3-O-glucoside˃petunidin-3-O-glucoside) whereas the mixtures were ineffective at the assayed range concentration. In a broader concentration range (0-2000 μg/mL), extracts did not show significant inhibition on porcine PL activity, but most of the pure anthocyanins were effective (petunidin-3-O-glucoside˃cyanidin-3-O-glucoside˃delphinidin-3-O-glucoside˃malvidin-3-O-glucoside). No inhibition was observed for peonidin-3-O-glucoside and mixtures of compounds. In summary, anthocyanins would contribute only marginally to the in vitro α-G inhibition. Regarding PL activity, their inhibitory capacity was reduced when present in extracts or mixtures, suggesting complex interactions with other components under the in vitro used conditions. In vivo studies are needed to confirm the effects of extracts/ pure anthocyanins on enzymatic activities observed here.
Fil: Mosse, Juana Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analitica y Fisicoquímica. Cátedra de Fisicoquímica; Argentina
Fil: Galleano, Mónica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analitica y Fisicoquímica. Cátedra de Fisicoquímica; Argentina
Fil: Fraga, César Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analitica y Fisicoquímica. Cátedra de Fisicoquímica; Argentina
LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Asociación Argentina de Fisiología
Ciudad Autonoma de Buenos Aires
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Asociación Argentina de Fisiología
description Food components providing glucose and fat-lowering effects in the body can be useful to attenuate the negative health effects associated to high-carbohydrates and/or high-fat diets. Here, anthocyanins were studied as potential inhibitors of α-glucosidase (α-G) and pancreatic lipase (PL) activities, two key enzymes responsible for carbohydrates and fats digestion. The anthocyanin profile of extracts obtained from bilberry (BB), blackcurrant (BC) and black rice (BR) was characterized. The capacity of extracts, pure anthocyanins, and mixtures (representative of anthocyanin extracts composition) to inhibit the activity of α-G and PL was evaluated using enzymatic assays, including acarbose and orlistat as positive controls for α-G and PL, respectively. In a concentration range of 20-320 μg/ mL, extracts showed a dose-dependent inhibitory effects on α-G activity. The calculated IC50 were 87 ± 7, 76 ± 7 and 236 ± 25 μg/mL for BB, BC and BR, respectively. Some pure anthocyanins showed inhibitory actions (cyanidin-3-O-glucoside˃delphinidin-3-O-glucoside˃petunidin-3-O-glucoside) whereas the mixtures were ineffective at the assayed range concentration. In a broader concentration range (0-2000 μg/mL), extracts did not show significant inhibition on porcine PL activity, but most of the pure anthocyanins were effective (petunidin-3-O-glucoside˃cyanidin-3-O-glucoside˃delphinidin-3-O-glucoside˃malvidin-3-O-glucoside). No inhibition was observed for peonidin-3-O-glucoside and mixtures of compounds. In summary, anthocyanins would contribute only marginally to the in vitro α-G inhibition. Regarding PL activity, their inhibitory capacity was reduced when present in extracts or mixtures, suggesting complex interactions with other components under the in vitro used conditions. In vivo studies are needed to confirm the effects of extracts/ pure anthocyanins on enzymatic activities observed here.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199438
Anthocyanins as inhibitors of α-glucosidase and pancreatic lipase activity; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Asociación Argentina de Fisiología; Ciudad Autonoma de Buenos Aires; Argentina; 2020; 1-3
0025-7680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199438
identifier_str_mv Anthocyanins as inhibitors of α-glucosidase and pancreatic lipase activity; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Asociación Argentina de Fisiología; Ciudad Autonoma de Buenos Aires; Argentina; 2020; 1-3
0025-7680
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundacion Revista Medicina
publisher.none.fl_str_mv Fundacion Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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