Dispersed Ascorbyl palmitate (ASC16) as an additive in adjuvant formulations

Autores
Sánchez Maslovski, Franco Martín; Alonso, Maria del Rosario; López, Gisela Lumila; Leiva, Laura Cristina Ana; Fusco, Luciano Sebastian
Año de publicación
2024
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Different aspects determine the choice of an adjuvant to generate optimal antibody productionwhile ensuring the safety of the producing animal. In this context, ASC16 that can form safehydrogels or act as a toxin inhibitor depending on whether it is present at high or low concentrations, respectively. For these reasons, it was proposed to evaluate the effect of dispersed ASC16 (low concentration) as an additive adjuvant in the production of experimental antivenom. BALB/c mice were immunised with Freund’s adjuvant in the presence or absence of additive (AFMo and AF) and hydrogel with or without additive (Pa40Mo and Pa40). Afterwards, ELISA, avidity andimmunoblotting tests were performed to determine the titre, binding strength and specificity ofthe antibodies. Finally, we determine the effective dose 50 (ED50) that neutralises PLA2 activityof Crotalus durissus terrificus venom. We used the t-tests for two independent samples to verifyif there were significant differences. Our results show that AFMo induced the highest titres (4.07),followed by AF and Pa40Mo (both 3.75), although without significant differences (p>0.05), and finally Pa40 (3.27). Notably, the additive ASC16 also improved antibody avidity, with AFMo (4.35 MKSCN) standing out with significant differences compared to the rest (p≤0.05), followed by AFand Pa40Mo (3.21 and 3.17 M KSCN) with no significant differences (p>0.05), and finally Pa40(1.61 M KSCN). In addition, the antibodies recognised the main antigenic components of thevenom. The sera produced by formulations with additive presented a better ED50 for PLA2 activity, highlighting Pa40Mo (7.04 µL), followed by AFMo (9.02 µL) with significant differences (p≤0.05) compared to Pa40 (12.20 µL) and AF (12.23 µL). This study shows that formulationswith additive ASC16 as an enhancer induce higher antibody titres, improve avidity, have a greaterspecificity and neutralising effect on PLA2 activity than formulations without the additive.
Fil: Sánchez Maslovski, Franco Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Alonso, Maria del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: López, Gisela Lumila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Leiva, Laura Cristina Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Fusco, Luciano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
14th Latin American and Caribbean Congress of Immunology
Buenos aires
Argentina
Sociedad Argentina de Inmunología
Latin America and Caribbean Association of Immunology
Asociación de Inmunólogos Clínicos de Argentina
Materia
ASC16
ADJUVANT
ADDITIVE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/275566
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/275566
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dispersed Ascorbyl palmitate (ASC16) as an additive in adjuvant formulationsSánchez Maslovski, Franco MartínAlonso, Maria del RosarioLópez, Gisela LumilaLeiva, Laura Cristina AnaFusco, Luciano SebastianASC16ADJUVANTADDITIVEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Different aspects determine the choice of an adjuvant to generate optimal antibody productionwhile ensuring the safety of the producing animal. In this context, ASC16 that can form safehydrogels or act as a toxin inhibitor depending on whether it is present at high or low concentrations, respectively. For these reasons, it was proposed to evaluate the effect of dispersed ASC16 (low concentration) as an additive adjuvant in the production of experimental antivenom. BALB/c mice were immunised with Freund’s adjuvant in the presence or absence of additive (AFMo and AF) and hydrogel with or without additive (Pa40Mo and Pa40). Afterwards, ELISA, avidity andimmunoblotting tests were performed to determine the titre, binding strength and specificity ofthe antibodies. Finally, we determine the effective dose 50 (ED50) that neutralises PLA2 activityof Crotalus durissus terrificus venom. We used the t-tests for two independent samples to verifyif there were significant differences. Our results show that AFMo induced the highest titres (4.07),followed by AF and Pa40Mo (both 3.75), although without significant differences (p>0.05), and finally Pa40 (3.27). Notably, the additive ASC16 also improved antibody avidity, with AFMo (4.35 MKSCN) standing out with significant differences compared to the rest (p≤0.05), followed by AFand Pa40Mo (3.21 and 3.17 M KSCN) with no significant differences (p>0.05), and finally Pa40(1.61 M KSCN). In addition, the antibodies recognised the main antigenic components of thevenom. The sera produced by formulations with additive presented a better ED50 for PLA2 activity, highlighting Pa40Mo (7.04 µL), followed by AFMo (9.02 µL) with significant differences (p≤0.05) compared to Pa40 (12.20 µL) and AF (12.23 µL). This study shows that formulationswith additive ASC16 as an enhancer induce higher antibody titres, improve avidity, have a greaterspecificity and neutralising effect on PLA2 activity than formulations without the additive.Fil: Sánchez Maslovski, Franco Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Alonso, Maria del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: López, Gisela Lumila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Leiva, Laura Cristina Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Fusco, Luciano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina14th Latin American and Caribbean Congress of ImmunologyBuenos airesArgentinaSociedad Argentina de InmunologíaLatin America and Caribbean Association of ImmunologyAsociación de Inmunólogos Clínicos de ArgentinaSociedad Argentina de Inmunología2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/275566Dispersed Ascorbyl palmitate (ASC16) as an additive in adjuvant formulations; 14th Latin American and Caribbean Congress of Immunology; Buenos aires; Argentina; 2024; 356-356978-950-34-2435-3CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://sedici.unlp.edu.ar/handle/10915/173621info:eu-repo/semantics/altIdentifier/doi/10.35537/10915/173621Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-03T09:46:18Zoai:ri.conicet.gov.ar:11336/275566instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-03 09:46:19.123CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dispersed Ascorbyl palmitate (ASC16) as an additive in adjuvant formulations
title Dispersed Ascorbyl palmitate (ASC16) as an additive in adjuvant formulations
spellingShingle Dispersed Ascorbyl palmitate (ASC16) as an additive in adjuvant formulations
Sánchez Maslovski, Franco Martín
ASC16
ADJUVANT
ADDITIVE
title_short Dispersed Ascorbyl palmitate (ASC16) as an additive in adjuvant formulations
title_full Dispersed Ascorbyl palmitate (ASC16) as an additive in adjuvant formulations
title_fullStr Dispersed Ascorbyl palmitate (ASC16) as an additive in adjuvant formulations
title_full_unstemmed Dispersed Ascorbyl palmitate (ASC16) as an additive in adjuvant formulations
title_sort Dispersed Ascorbyl palmitate (ASC16) as an additive in adjuvant formulations
dc.creator.none.fl_str_mv Sánchez Maslovski, Franco Martín
Alonso, Maria del Rosario
López, Gisela Lumila
Leiva, Laura Cristina Ana
Fusco, Luciano Sebastian
author Sánchez Maslovski, Franco Martín
author_facet Sánchez Maslovski, Franco Martín
Alonso, Maria del Rosario
López, Gisela Lumila
Leiva, Laura Cristina Ana
Fusco, Luciano Sebastian
author_role author
author2 Alonso, Maria del Rosario
López, Gisela Lumila
Leiva, Laura Cristina Ana
Fusco, Luciano Sebastian
author2_role author
author
author
author
dc.subject.none.fl_str_mv ASC16
ADJUVANT
ADDITIVE
topic ASC16
ADJUVANT
ADDITIVE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Different aspects determine the choice of an adjuvant to generate optimal antibody productionwhile ensuring the safety of the producing animal. In this context, ASC16 that can form safehydrogels or act as a toxin inhibitor depending on whether it is present at high or low concentrations, respectively. For these reasons, it was proposed to evaluate the effect of dispersed ASC16 (low concentration) as an additive adjuvant in the production of experimental antivenom. BALB/c mice were immunised with Freund’s adjuvant in the presence or absence of additive (AFMo and AF) and hydrogel with or without additive (Pa40Mo and Pa40). Afterwards, ELISA, avidity andimmunoblotting tests were performed to determine the titre, binding strength and specificity ofthe antibodies. Finally, we determine the effective dose 50 (ED50) that neutralises PLA2 activityof Crotalus durissus terrificus venom. We used the t-tests for two independent samples to verifyif there were significant differences. Our results show that AFMo induced the highest titres (4.07),followed by AF and Pa40Mo (both 3.75), although without significant differences (p>0.05), and finally Pa40 (3.27). Notably, the additive ASC16 also improved antibody avidity, with AFMo (4.35 MKSCN) standing out with significant differences compared to the rest (p≤0.05), followed by AFand Pa40Mo (3.21 and 3.17 M KSCN) with no significant differences (p>0.05), and finally Pa40(1.61 M KSCN). In addition, the antibodies recognised the main antigenic components of thevenom. The sera produced by formulations with additive presented a better ED50 for PLA2 activity, highlighting Pa40Mo (7.04 µL), followed by AFMo (9.02 µL) with significant differences (p≤0.05) compared to Pa40 (12.20 µL) and AF (12.23 µL). This study shows that formulationswith additive ASC16 as an enhancer induce higher antibody titres, improve avidity, have a greaterspecificity and neutralising effect on PLA2 activity than formulations without the additive.
Fil: Sánchez Maslovski, Franco Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Alonso, Maria del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: López, Gisela Lumila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Leiva, Laura Cristina Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Fusco, Luciano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
14th Latin American and Caribbean Congress of Immunology
Buenos aires
Argentina
Sociedad Argentina de Inmunología
Latin America and Caribbean Association of Immunology
Asociación de Inmunólogos Clínicos de Argentina
description Different aspects determine the choice of an adjuvant to generate optimal antibody productionwhile ensuring the safety of the producing animal. In this context, ASC16 that can form safehydrogels or act as a toxin inhibitor depending on whether it is present at high or low concentrations, respectively. For these reasons, it was proposed to evaluate the effect of dispersed ASC16 (low concentration) as an additive adjuvant in the production of experimental antivenom. BALB/c mice were immunised with Freund’s adjuvant in the presence or absence of additive (AFMo and AF) and hydrogel with or without additive (Pa40Mo and Pa40). Afterwards, ELISA, avidity andimmunoblotting tests were performed to determine the titre, binding strength and specificity ofthe antibodies. Finally, we determine the effective dose 50 (ED50) that neutralises PLA2 activityof Crotalus durissus terrificus venom. We used the t-tests for two independent samples to verifyif there were significant differences. Our results show that AFMo induced the highest titres (4.07),followed by AF and Pa40Mo (both 3.75), although without significant differences (p>0.05), and finally Pa40 (3.27). Notably, the additive ASC16 also improved antibody avidity, with AFMo (4.35 MKSCN) standing out with significant differences compared to the rest (p≤0.05), followed by AFand Pa40Mo (3.21 and 3.17 M KSCN) with no significant differences (p>0.05), and finally Pa40(1.61 M KSCN). In addition, the antibodies recognised the main antigenic components of thevenom. The sera produced by formulations with additive presented a better ED50 for PLA2 activity, highlighting Pa40Mo (7.04 µL), followed by AFMo (9.02 µL) with significant differences (p≤0.05) compared to Pa40 (12.20 µL) and AF (12.23 µL). This study shows that formulationswith additive ASC16 as an enhancer induce higher antibody titres, improve avidity, have a greaterspecificity and neutralising effect on PLA2 activity than formulations without the additive.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/275566
Dispersed Ascorbyl palmitate (ASC16) as an additive in adjuvant formulations; 14th Latin American and Caribbean Congress of Immunology; Buenos aires; Argentina; 2024; 356-356
978-950-34-2435-3
CONICET Digital
CONICET
url http://hdl.handle.net/11336/275566
identifier_str_mv Dispersed Ascorbyl palmitate (ASC16) as an additive in adjuvant formulations; 14th Latin American and Caribbean Congress of Immunology; Buenos aires; Argentina; 2024; 356-356
978-950-34-2435-3
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.35537/10915/173621
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Inmunología
publisher.none.fl_str_mv Sociedad Argentina de Inmunología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.275514

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