Kinesin KIF4A transports integrin β1 in developing axons of cortical neurons
- Autores
- Heinzt, Tristan; Heller, Janosh P.; Zhao, Rongrong; Caceres, Alfredo Oscar; Eva, RIchard; Fawcett, James E.
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- CNS axons have poor regenerative ability compared to PNS axons, and mature axons regenerate less well than immature embryonic axons. The loss of regenerative ability with maturity is accompanied by the setting up of a selective transport filter in axons, restricting the types of molecule that are present. We confirm that integrins (represented by subunits β1 and α5) are present in early cortical axons in vitro but are excluded from mature axons. Ribosomal protein and L1 show selective axonal transport through association with kinesin kif4A; we have therefore examined the hypothesis that integrin transport might also be in association with kif4A. Kif4A is present in all processes of immature cortical neurons cultured at E18, then downregulated by 14 days in vitro, coinciding with the exclusion of integrin from axons. Kif4a co-localises with β1 integrin in vesicles in neurons and non-neuronal cells, and the two molecules co-immunoprecipitate. Knockdown of KIF4A expression with shRNA reduced the level of integrin β1 in axons of developing neurons and reduced neurite elongation on laminin, an integrin-dependent substrate. Overexpression of kif4A triggered apoptosis in neuronal and non-neuronal cells. In mature neurons expression of kif4A-GFP at a modest level did not kill the cells, and the kif4A was detectable in their axons. However this was not accompanied by an increase in integrin β1 axonal transport, suggesting that kif4A is not the only integrin transporter, and that integrin exclusion from axons is controlled by factors other than the kif4A level.
Fil: Heinzt, Tristan. University of Cambridge; Reino Unido
Fil: Heller, Janosh P.. University of Cambridge; Reino Unido
Fil: Zhao, Rongrong. University of Cambridge; Reino Unido
Fil: Caceres, Alfredo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Eva, RIchard. University of Cambridge; Reino Unido
Fil: Fawcett, James E.. University of Cambridge; Reino Unido - Materia
-
Axon
Axon Transport
Axon Growth
Integrin
Trafficking
Kinesin - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/31638
Ver los metadatos del registro completo
id |
CONICETDig_4ef83fd401680e3632c376503a87c332 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/31638 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Kinesin KIF4A transports integrin β1 in developing axons of cortical neuronsHeinzt, TristanHeller, Janosh P.Zhao, RongrongCaceres, Alfredo OscarEva, RIchardFawcett, James E.AxonAxon TransportAxon GrowthIntegrinTraffickingKinesinhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3CNS axons have poor regenerative ability compared to PNS axons, and mature axons regenerate less well than immature embryonic axons. The loss of regenerative ability with maturity is accompanied by the setting up of a selective transport filter in axons, restricting the types of molecule that are present. We confirm that integrins (represented by subunits β1 and α5) are present in early cortical axons in vitro but are excluded from mature axons. Ribosomal protein and L1 show selective axonal transport through association with kinesin kif4A; we have therefore examined the hypothesis that integrin transport might also be in association with kif4A. Kif4A is present in all processes of immature cortical neurons cultured at E18, then downregulated by 14 days in vitro, coinciding with the exclusion of integrin from axons. Kif4a co-localises with β1 integrin in vesicles in neurons and non-neuronal cells, and the two molecules co-immunoprecipitate. Knockdown of KIF4A expression with shRNA reduced the level of integrin β1 in axons of developing neurons and reduced neurite elongation on laminin, an integrin-dependent substrate. Overexpression of kif4A triggered apoptosis in neuronal and non-neuronal cells. In mature neurons expression of kif4A-GFP at a modest level did not kill the cells, and the kif4A was detectable in their axons. However this was not accompanied by an increase in integrin β1 axonal transport, suggesting that kif4A is not the only integrin transporter, and that integrin exclusion from axons is controlled by factors other than the kif4A level.Fil: Heinzt, Tristan. University of Cambridge; Reino UnidoFil: Heller, Janosh P.. University of Cambridge; Reino UnidoFil: Zhao, Rongrong. University of Cambridge; Reino UnidoFil: Caceres, Alfredo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Eva, RIchard. University of Cambridge; Reino UnidoFil: Fawcett, James E.. University of Cambridge; Reino UnidoAcademic Press Inc Elsevier Science2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31638Fawcett, James E.; Caceres, Alfredo Oscar; Heinzt, Tristan; Zhao, Rongrong; Heller, Janosh P.; Eva, RIchard; et al.; Kinesin KIF4A transports integrin β1 in developing axons of cortical neurons; Academic Press Inc Elsevier Science; Molecular and Cellular Neuroscience; 63; 9-2014; 60-711044-7431CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mcn.2014.09.003info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1044743114001638info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:30:27Zoai:ri.conicet.gov.ar:11336/31638instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:30:28.077CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Kinesin KIF4A transports integrin β1 in developing axons of cortical neurons |
title |
Kinesin KIF4A transports integrin β1 in developing axons of cortical neurons |
spellingShingle |
Kinesin KIF4A transports integrin β1 in developing axons of cortical neurons Heinzt, Tristan Axon Axon Transport Axon Growth Integrin Trafficking Kinesin |
title_short |
Kinesin KIF4A transports integrin β1 in developing axons of cortical neurons |
title_full |
Kinesin KIF4A transports integrin β1 in developing axons of cortical neurons |
title_fullStr |
Kinesin KIF4A transports integrin β1 in developing axons of cortical neurons |
title_full_unstemmed |
Kinesin KIF4A transports integrin β1 in developing axons of cortical neurons |
title_sort |
Kinesin KIF4A transports integrin β1 in developing axons of cortical neurons |
dc.creator.none.fl_str_mv |
Heinzt, Tristan Heller, Janosh P. Zhao, Rongrong Caceres, Alfredo Oscar Eva, RIchard Fawcett, James E. |
author |
Heinzt, Tristan |
author_facet |
Heinzt, Tristan Heller, Janosh P. Zhao, Rongrong Caceres, Alfredo Oscar Eva, RIchard Fawcett, James E. |
author_role |
author |
author2 |
Heller, Janosh P. Zhao, Rongrong Caceres, Alfredo Oscar Eva, RIchard Fawcett, James E. |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Axon Axon Transport Axon Growth Integrin Trafficking Kinesin |
topic |
Axon Axon Transport Axon Growth Integrin Trafficking Kinesin |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
CNS axons have poor regenerative ability compared to PNS axons, and mature axons regenerate less well than immature embryonic axons. The loss of regenerative ability with maturity is accompanied by the setting up of a selective transport filter in axons, restricting the types of molecule that are present. We confirm that integrins (represented by subunits β1 and α5) are present in early cortical axons in vitro but are excluded from mature axons. Ribosomal protein and L1 show selective axonal transport through association with kinesin kif4A; we have therefore examined the hypothesis that integrin transport might also be in association with kif4A. Kif4A is present in all processes of immature cortical neurons cultured at E18, then downregulated by 14 days in vitro, coinciding with the exclusion of integrin from axons. Kif4a co-localises with β1 integrin in vesicles in neurons and non-neuronal cells, and the two molecules co-immunoprecipitate. Knockdown of KIF4A expression with shRNA reduced the level of integrin β1 in axons of developing neurons and reduced neurite elongation on laminin, an integrin-dependent substrate. Overexpression of kif4A triggered apoptosis in neuronal and non-neuronal cells. In mature neurons expression of kif4A-GFP at a modest level did not kill the cells, and the kif4A was detectable in their axons. However this was not accompanied by an increase in integrin β1 axonal transport, suggesting that kif4A is not the only integrin transporter, and that integrin exclusion from axons is controlled by factors other than the kif4A level. Fil: Heinzt, Tristan. University of Cambridge; Reino Unido Fil: Heller, Janosh P.. University of Cambridge; Reino Unido Fil: Zhao, Rongrong. University of Cambridge; Reino Unido Fil: Caceres, Alfredo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina Fil: Eva, RIchard. University of Cambridge; Reino Unido Fil: Fawcett, James E.. University of Cambridge; Reino Unido |
description |
CNS axons have poor regenerative ability compared to PNS axons, and mature axons regenerate less well than immature embryonic axons. The loss of regenerative ability with maturity is accompanied by the setting up of a selective transport filter in axons, restricting the types of molecule that are present. We confirm that integrins (represented by subunits β1 and α5) are present in early cortical axons in vitro but are excluded from mature axons. Ribosomal protein and L1 show selective axonal transport through association with kinesin kif4A; we have therefore examined the hypothesis that integrin transport might also be in association with kif4A. Kif4A is present in all processes of immature cortical neurons cultured at E18, then downregulated by 14 days in vitro, coinciding with the exclusion of integrin from axons. Kif4a co-localises with β1 integrin in vesicles in neurons and non-neuronal cells, and the two molecules co-immunoprecipitate. Knockdown of KIF4A expression with shRNA reduced the level of integrin β1 in axons of developing neurons and reduced neurite elongation on laminin, an integrin-dependent substrate. Overexpression of kif4A triggered apoptosis in neuronal and non-neuronal cells. In mature neurons expression of kif4A-GFP at a modest level did not kill the cells, and the kif4A was detectable in their axons. However this was not accompanied by an increase in integrin β1 axonal transport, suggesting that kif4A is not the only integrin transporter, and that integrin exclusion from axons is controlled by factors other than the kif4A level. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/31638 Fawcett, James E.; Caceres, Alfredo Oscar; Heinzt, Tristan; Zhao, Rongrong; Heller, Janosh P.; Eva, RIchard; et al.; Kinesin KIF4A transports integrin β1 in developing axons of cortical neurons; Academic Press Inc Elsevier Science; Molecular and Cellular Neuroscience; 63; 9-2014; 60-71 1044-7431 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/31638 |
identifier_str_mv |
Fawcett, James E.; Caceres, Alfredo Oscar; Heinzt, Tristan; Zhao, Rongrong; Heller, Janosh P.; Eva, RIchard; et al.; Kinesin KIF4A transports integrin β1 in developing axons of cortical neurons; Academic Press Inc Elsevier Science; Molecular and Cellular Neuroscience; 63; 9-2014; 60-71 1044-7431 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mcn.2014.09.003 info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1044743114001638 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Academic Press Inc Elsevier Science |
publisher.none.fl_str_mv |
Academic Press Inc Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1846083442738987008 |
score |
13.22299 |