Positional differences of axon growth rates between sensory neurons encoded by runx3

Autores
Lallemend, Francois; Sterzenbach, Ulrich; Hadjab Lallemend, Saida; Aquino, Jorge Benjamin; Castelo Branco, Goncalo; Sinha, Indranil; Villaescusa, J. Carlos; Levanon, Ditsa; Wang, Yiqiao; Franck, Marina C. M.; Kharchenko, Olga; Adameyko, Igor; Linnarsson, Sten; Groner, Yoram; Turner, Eric; Ernfors, Patrik
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The formation of functional connectivity in the nervous system is governed by axon guidance that instructs nerve growth and branching during development, implying a similarity between neuronal subtypes in terms of nerve extension. We demonstrate the molecular mechanism of another layer of complexity in vertebrates by defining a transcriptional program underlying growth differences between positionally different neurons. The rate of axon extension of the early subset of embryonic dorsal root ganglion sensory neurons is encoded in neurons at different axial levels. This code is determined by a segmental pattern of axial levels of Runx family transcription factor Runx3. Runx3 in turn determines transcription levels of genes encoding cytoskeletal proteins involved in axon extension, including Rock1 and Rock2 which have ongoing activities determining axon growth in early sensory neurons and blocking Rock activity reverses axon extension deficits of Runx3−/− neurons. Thus, Runx3 acts to regulate positional differences in axon extension properties apparently without affecting nerve guidance and branching, a principle that could be relevant to other parts of the nervous system.
Fil: Lallemend, Francois. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Sterzenbach, Ulrich. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Hadjab Lallemend, Saida. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Aquino, Jorge Benjamin. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Castelo Branco, Goncalo. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Sinha, Indranil. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Villaescusa, J. Carlos. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Levanon, Ditsa. The Weizmann Institute of Science; Israel
Fil: Wang, Yiqiao. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Franck, Marina C. M.. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Kharchenko, Olga. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Adameyko, Igor. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Linnarsson, Sten. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Groner, Yoram. The Weizmann Institute of Science; Israel
Fil: Turner, Eric. Seattle Children's Research Institute; Estados Unidos
Fil: Ernfors, Patrik. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Materia
AXON GROWTH
DEVELOPMENT
INTRINSIC
RUNX3
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96181
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/96181
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Positional differences of axon growth rates between sensory neurons encoded by runx3Lallemend, FrancoisSterzenbach, UlrichHadjab Lallemend, SaidaAquino, Jorge BenjaminCastelo Branco, GoncaloSinha, IndranilVillaescusa, J. CarlosLevanon, DitsaWang, YiqiaoFranck, Marina C. M.Kharchenko, OlgaAdameyko, IgorLinnarsson, StenGroner, YoramTurner, EricErnfors, PatrikAXON GROWTHDEVELOPMENTINTRINSICRUNX3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The formation of functional connectivity in the nervous system is governed by axon guidance that instructs nerve growth and branching during development, implying a similarity between neuronal subtypes in terms of nerve extension. We demonstrate the molecular mechanism of another layer of complexity in vertebrates by defining a transcriptional program underlying growth differences between positionally different neurons. The rate of axon extension of the early subset of embryonic dorsal root ganglion sensory neurons is encoded in neurons at different axial levels. This code is determined by a segmental pattern of axial levels of Runx family transcription factor Runx3. Runx3 in turn determines transcription levels of genes encoding cytoskeletal proteins involved in axon extension, including Rock1 and Rock2 which have ongoing activities determining axon growth in early sensory neurons and blocking Rock activity reverses axon extension deficits of Runx3−/− neurons. Thus, Runx3 acts to regulate positional differences in axon extension properties apparently without affecting nerve guidance and branching, a principle that could be relevant to other parts of the nervous system.Fil: Lallemend, Francois. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Sterzenbach, Ulrich. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Hadjab Lallemend, Saida. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Aquino, Jorge Benjamin. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Castelo Branco, Goncalo. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Sinha, Indranil. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Villaescusa, J. Carlos. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Levanon, Ditsa. The Weizmann Institute of Science; IsraelFil: Wang, Yiqiao. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Franck, Marina C. M.. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Kharchenko, Olga. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Adameyko, Igor. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Linnarsson, Sten. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Groner, Yoram. The Weizmann Institute of Science; IsraelFil: Turner, Eric. Seattle Children's Research Institute; Estados UnidosFil: Ernfors, Patrik. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaNature Publishing Group2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96181Lallemend, Francois; Sterzenbach, Ulrich; Hadjab Lallemend, Saida; Aquino, Jorge Benjamin; Castelo Branco, Goncalo; et al.; Positional differences of axon growth rates between sensory neurons encoded by runx3; Nature Publishing Group; Embo Journal; 31; 18; 9-2012; 3718-37290261-4189CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.embopress.org/doi/10.1038/emboj.2012.228info:eu-repo/semantics/altIdentifier/doi/10.1038%2Femboj.2012.228info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3442276/#!po=75.0000info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:43Zoai:ri.conicet.gov.ar:11336/96181instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:43.469CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Positional differences of axon growth rates between sensory neurons encoded by runx3
title Positional differences of axon growth rates between sensory neurons encoded by runx3
spellingShingle Positional differences of axon growth rates between sensory neurons encoded by runx3
Lallemend, Francois
AXON GROWTH
DEVELOPMENT
INTRINSIC
RUNX3
title_short Positional differences of axon growth rates between sensory neurons encoded by runx3
title_full Positional differences of axon growth rates between sensory neurons encoded by runx3
title_fullStr Positional differences of axon growth rates between sensory neurons encoded by runx3
title_full_unstemmed Positional differences of axon growth rates between sensory neurons encoded by runx3
title_sort Positional differences of axon growth rates between sensory neurons encoded by runx3
dc.creator.none.fl_str_mv Lallemend, Francois
Sterzenbach, Ulrich
Hadjab Lallemend, Saida
Aquino, Jorge Benjamin
Castelo Branco, Goncalo
Sinha, Indranil
Villaescusa, J. Carlos
Levanon, Ditsa
Wang, Yiqiao
Franck, Marina C. M.
Kharchenko, Olga
Adameyko, Igor
Linnarsson, Sten
Groner, Yoram
Turner, Eric
Ernfors, Patrik
author Lallemend, Francois
author_facet Lallemend, Francois
Sterzenbach, Ulrich
Hadjab Lallemend, Saida
Aquino, Jorge Benjamin
Castelo Branco, Goncalo
Sinha, Indranil
Villaescusa, J. Carlos
Levanon, Ditsa
Wang, Yiqiao
Franck, Marina C. M.
Kharchenko, Olga
Adameyko, Igor
Linnarsson, Sten
Groner, Yoram
Turner, Eric
Ernfors, Patrik
author_role author
author2 Sterzenbach, Ulrich
Hadjab Lallemend, Saida
Aquino, Jorge Benjamin
Castelo Branco, Goncalo
Sinha, Indranil
Villaescusa, J. Carlos
Levanon, Ditsa
Wang, Yiqiao
Franck, Marina C. M.
Kharchenko, Olga
Adameyko, Igor
Linnarsson, Sten
Groner, Yoram
Turner, Eric
Ernfors, Patrik
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AXON GROWTH
DEVELOPMENT
INTRINSIC
RUNX3
topic AXON GROWTH
DEVELOPMENT
INTRINSIC
RUNX3
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The formation of functional connectivity in the nervous system is governed by axon guidance that instructs nerve growth and branching during development, implying a similarity between neuronal subtypes in terms of nerve extension. We demonstrate the molecular mechanism of another layer of complexity in vertebrates by defining a transcriptional program underlying growth differences between positionally different neurons. The rate of axon extension of the early subset of embryonic dorsal root ganglion sensory neurons is encoded in neurons at different axial levels. This code is determined by a segmental pattern of axial levels of Runx family transcription factor Runx3. Runx3 in turn determines transcription levels of genes encoding cytoskeletal proteins involved in axon extension, including Rock1 and Rock2 which have ongoing activities determining axon growth in early sensory neurons and blocking Rock activity reverses axon extension deficits of Runx3−/− neurons. Thus, Runx3 acts to regulate positional differences in axon extension properties apparently without affecting nerve guidance and branching, a principle that could be relevant to other parts of the nervous system.
Fil: Lallemend, Francois. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Sterzenbach, Ulrich. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Hadjab Lallemend, Saida. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Aquino, Jorge Benjamin. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Castelo Branco, Goncalo. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Sinha, Indranil. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Villaescusa, J. Carlos. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Levanon, Ditsa. The Weizmann Institute of Science; Israel
Fil: Wang, Yiqiao. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Franck, Marina C. M.. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Kharchenko, Olga. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Adameyko, Igor. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Linnarsson, Sten. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Groner, Yoram. The Weizmann Institute of Science; Israel
Fil: Turner, Eric. Seattle Children's Research Institute; Estados Unidos
Fil: Ernfors, Patrik. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
description The formation of functional connectivity in the nervous system is governed by axon guidance that instructs nerve growth and branching during development, implying a similarity between neuronal subtypes in terms of nerve extension. We demonstrate the molecular mechanism of another layer of complexity in vertebrates by defining a transcriptional program underlying growth differences between positionally different neurons. The rate of axon extension of the early subset of embryonic dorsal root ganglion sensory neurons is encoded in neurons at different axial levels. This code is determined by a segmental pattern of axial levels of Runx family transcription factor Runx3. Runx3 in turn determines transcription levels of genes encoding cytoskeletal proteins involved in axon extension, including Rock1 and Rock2 which have ongoing activities determining axon growth in early sensory neurons and blocking Rock activity reverses axon extension deficits of Runx3−/− neurons. Thus, Runx3 acts to regulate positional differences in axon extension properties apparently without affecting nerve guidance and branching, a principle that could be relevant to other parts of the nervous system.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96181
Lallemend, Francois; Sterzenbach, Ulrich; Hadjab Lallemend, Saida; Aquino, Jorge Benjamin; Castelo Branco, Goncalo; et al.; Positional differences of axon growth rates between sensory neurons encoded by runx3; Nature Publishing Group; Embo Journal; 31; 18; 9-2012; 3718-3729
0261-4189
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96181
identifier_str_mv Lallemend, Francois; Sterzenbach, Ulrich; Hadjab Lallemend, Saida; Aquino, Jorge Benjamin; Castelo Branco, Goncalo; et al.; Positional differences of axon growth rates between sensory neurons encoded by runx3; Nature Publishing Group; Embo Journal; 31; 18; 9-2012; 3718-3729
0261-4189
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.embopress.org/doi/10.1038/emboj.2012.228
info:eu-repo/semantics/altIdentifier/doi/10.1038%2Femboj.2012.228
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3442276/#!po=75.0000
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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