Facile synthesis of antibiotic-functionalized gold nanoparticles for colorimetric bacterial detection

Autores
Elliott, Charlotte N.; Becerra, María Cecilia; Craig, Bennett, J.; Graham, Lori; Silvero, María Jazmín; Hallett Tapley, Geniece Hallett-tapley
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The development of quick and efficient methods for the detection of pathogenic bacteria is urgently needed for the diagnosis of infectious diseases and the control of microbiological contamination in global waterways, potable water sources and the food industry. This contribution will describe the synthesis of gold nanoparticles and their conjugation to broad spectrum, polypeptide and ß-lactam antibiotics that function as both reducing agents and surface protectants (ATB@AuNP). Nanoparticle colloids examined using transmission electron microscopy are generally spherical in shape and range from 2-50 nm in size. Dynamic light scattering and infrared spectroscopy were also used to confirm encapsulation of the AuNP surface by antibiotic molecules. ATB@AuNP were then used to detect 3 common pathogenic bacterial species:Staphylococcus aureus, Pseudomonas aeruginosa,andEscherichia coli. The colour of the AuNP colloid was monitored visually and using UV-visible spectroscopy. A red shift of the UV visible absorbance and a visible colour change following introduction of each pathogen is indicative of ATB binding to the bacteria surface, ascribed to AuNP agglomeration. This work demonstrates that ATB@AuNP may be an efficient and high throughput tool for the rapid detection of common bacterial contaminants.
Fil: Elliott, Charlotte N.. St. Francis Xavier University; Canadá
Fil: Becerra, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinar de Biología Vegetal (P). Grupo Vinculado Centro de Relevamiento y Evaluación de Recursos Agrícolas y Naturales; Argentina
Fil: Craig, Bennett, J.. Acadia University; Canadá
Fil: Graham, Lori. St. Francis Xavier University; Canadá
Fil: Silvero, María Jazmín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Hallett Tapley, Geniece Hallett-tapley. St. Francis Xavier University; Canadá
Materia
BACTERIA
DETECTION
GOLD
NANOPARTICLE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/137616
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Facile synthesis of antibiotic-functionalized gold nanoparticles for colorimetric bacterial detectionElliott, Charlotte N.Becerra, María CeciliaCraig, Bennett, J.Graham, LoriSilvero, María JazmínHallett Tapley, Geniece Hallett-tapleyBACTERIADETECTIONGOLDNANOPARTICLEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The development of quick and efficient methods for the detection of pathogenic bacteria is urgently needed for the diagnosis of infectious diseases and the control of microbiological contamination in global waterways, potable water sources and the food industry. This contribution will describe the synthesis of gold nanoparticles and their conjugation to broad spectrum, polypeptide and ß-lactam antibiotics that function as both reducing agents and surface protectants (ATB@AuNP). Nanoparticle colloids examined using transmission electron microscopy are generally spherical in shape and range from 2-50 nm in size. Dynamic light scattering and infrared spectroscopy were also used to confirm encapsulation of the AuNP surface by antibiotic molecules. ATB@AuNP were then used to detect 3 common pathogenic bacterial species:Staphylococcus aureus, Pseudomonas aeruginosa,andEscherichia coli. The colour of the AuNP colloid was monitored visually and using UV-visible spectroscopy. A red shift of the UV visible absorbance and a visible colour change following introduction of each pathogen is indicative of ATB binding to the bacteria surface, ascribed to AuNP agglomeration. This work demonstrates that ATB@AuNP may be an efficient and high throughput tool for the rapid detection of common bacterial contaminants.Fil: Elliott, Charlotte N.. St. Francis Xavier University; CanadáFil: Becerra, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinar de Biología Vegetal (P). Grupo Vinculado Centro de Relevamiento y Evaluación de Recursos Agrícolas y Naturales; ArgentinaFil: Craig, Bennett, J.. Acadia University; CanadáFil: Graham, Lori. St. Francis Xavier University; CanadáFil: Silvero, María Jazmín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Hallett Tapley, Geniece Hallett-tapley. St. Francis Xavier University; CanadáRoyal Society of Chemistry2021-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/137616Elliott, Charlotte N.; Becerra, María Cecilia; Craig, Bennett, J.; Graham, Lori; Silvero, María Jazmín; et al.; Facile synthesis of antibiotic-functionalized gold nanoparticles for colorimetric bacterial detection; Royal Society of Chemistry; RSC Advances; 11; 23; 4-2021; 14161-141682046-2069CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1039/d1ra01316einfo:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2021/RA/D1RA01316Einfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:31Zoai:ri.conicet.gov.ar:11336/137616instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:31.226CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Facile synthesis of antibiotic-functionalized gold nanoparticles for colorimetric bacterial detection
title Facile synthesis of antibiotic-functionalized gold nanoparticles for colorimetric bacterial detection
spellingShingle Facile synthesis of antibiotic-functionalized gold nanoparticles for colorimetric bacterial detection
Elliott, Charlotte N.
BACTERIA
DETECTION
GOLD
NANOPARTICLE
title_short Facile synthesis of antibiotic-functionalized gold nanoparticles for colorimetric bacterial detection
title_full Facile synthesis of antibiotic-functionalized gold nanoparticles for colorimetric bacterial detection
title_fullStr Facile synthesis of antibiotic-functionalized gold nanoparticles for colorimetric bacterial detection
title_full_unstemmed Facile synthesis of antibiotic-functionalized gold nanoparticles for colorimetric bacterial detection
title_sort Facile synthesis of antibiotic-functionalized gold nanoparticles for colorimetric bacterial detection
dc.creator.none.fl_str_mv Elliott, Charlotte N.
Becerra, María Cecilia
Craig, Bennett, J.
Graham, Lori
Silvero, María Jazmín
Hallett Tapley, Geniece Hallett-tapley
author Elliott, Charlotte N.
author_facet Elliott, Charlotte N.
Becerra, María Cecilia
Craig, Bennett, J.
Graham, Lori
Silvero, María Jazmín
Hallett Tapley, Geniece Hallett-tapley
author_role author
author2 Becerra, María Cecilia
Craig, Bennett, J.
Graham, Lori
Silvero, María Jazmín
Hallett Tapley, Geniece Hallett-tapley
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BACTERIA
DETECTION
GOLD
NANOPARTICLE
topic BACTERIA
DETECTION
GOLD
NANOPARTICLE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The development of quick and efficient methods for the detection of pathogenic bacteria is urgently needed for the diagnosis of infectious diseases and the control of microbiological contamination in global waterways, potable water sources and the food industry. This contribution will describe the synthesis of gold nanoparticles and their conjugation to broad spectrum, polypeptide and ß-lactam antibiotics that function as both reducing agents and surface protectants (ATB@AuNP). Nanoparticle colloids examined using transmission electron microscopy are generally spherical in shape and range from 2-50 nm in size. Dynamic light scattering and infrared spectroscopy were also used to confirm encapsulation of the AuNP surface by antibiotic molecules. ATB@AuNP were then used to detect 3 common pathogenic bacterial species:Staphylococcus aureus, Pseudomonas aeruginosa,andEscherichia coli. The colour of the AuNP colloid was monitored visually and using UV-visible spectroscopy. A red shift of the UV visible absorbance and a visible colour change following introduction of each pathogen is indicative of ATB binding to the bacteria surface, ascribed to AuNP agglomeration. This work demonstrates that ATB@AuNP may be an efficient and high throughput tool for the rapid detection of common bacterial contaminants.
Fil: Elliott, Charlotte N.. St. Francis Xavier University; Canadá
Fil: Becerra, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinar de Biología Vegetal (P). Grupo Vinculado Centro de Relevamiento y Evaluación de Recursos Agrícolas y Naturales; Argentina
Fil: Craig, Bennett, J.. Acadia University; Canadá
Fil: Graham, Lori. St. Francis Xavier University; Canadá
Fil: Silvero, María Jazmín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Hallett Tapley, Geniece Hallett-tapley. St. Francis Xavier University; Canadá
description The development of quick and efficient methods for the detection of pathogenic bacteria is urgently needed for the diagnosis of infectious diseases and the control of microbiological contamination in global waterways, potable water sources and the food industry. This contribution will describe the synthesis of gold nanoparticles and their conjugation to broad spectrum, polypeptide and ß-lactam antibiotics that function as both reducing agents and surface protectants (ATB@AuNP). Nanoparticle colloids examined using transmission electron microscopy are generally spherical in shape and range from 2-50 nm in size. Dynamic light scattering and infrared spectroscopy were also used to confirm encapsulation of the AuNP surface by antibiotic molecules. ATB@AuNP were then used to detect 3 common pathogenic bacterial species:Staphylococcus aureus, Pseudomonas aeruginosa,andEscherichia coli. The colour of the AuNP colloid was monitored visually and using UV-visible spectroscopy. A red shift of the UV visible absorbance and a visible colour change following introduction of each pathogen is indicative of ATB binding to the bacteria surface, ascribed to AuNP agglomeration. This work demonstrates that ATB@AuNP may be an efficient and high throughput tool for the rapid detection of common bacterial contaminants.
publishDate 2021
dc.date.none.fl_str_mv 2021-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/137616
Elliott, Charlotte N.; Becerra, María Cecilia; Craig, Bennett, J.; Graham, Lori; Silvero, María Jazmín; et al.; Facile synthesis of antibiotic-functionalized gold nanoparticles for colorimetric bacterial detection; Royal Society of Chemistry; RSC Advances; 11; 23; 4-2021; 14161-14168
2046-2069
CONICET Digital
CONICET
url http://hdl.handle.net/11336/137616
identifier_str_mv Elliott, Charlotte N.; Becerra, María Cecilia; Craig, Bennett, J.; Graham, Lori; Silvero, María Jazmín; et al.; Facile synthesis of antibiotic-functionalized gold nanoparticles for colorimetric bacterial detection; Royal Society of Chemistry; RSC Advances; 11; 23; 4-2021; 14161-14168
2046-2069
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1039/d1ra01316e
info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2021/RA/D1RA01316E
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269700382261248
score 13.13397

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