Curcumin suppresses p38 mitogen-activated protein kinase activation, reduces IL-1β and matrix metalloproteinase-3 and enhances IL-10 in the mucosa of children and adults with infla...

Autores
Epstein, Jenny; Docena, Guillermo H.; MacDonald, Thomas T.; Sanderson, Ian R.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Inflammatory bowel disease (IBD) is a major source of morbidity in children and adults. Its incidence is rising, particularly in young people. IBD carries a lifelong risk of cancer which is proportional to disease duration. Drug and surgical treatments rarely offer cure and often carry a high side effect burden. Dietary therapy is highly effective in selected cases of Crohn’s disease. For these reasons there is much interest in developing novel dietary treatments in IBD. Curcumin, a component of the spice turmeric, and an anti-inflammatory and anti-cancer agent, shows preclinical and clinical potential in IBD. Its mechanisms of action are unknown. Our aim was to assess the effect of curcumin on key disease mediators p38 MAPK, IL-1â, IL-10 and MMP-3 in the gut of children and adults with IBD. Colonic mucosal biopsies and myofibroblasts (CMF) from children and adults with active IBD were cultured ex vivo with curcumin. p38 MAPK, NF-êB and MMP-3 were measured by immunoblotting. IL-1â and IL-10 were measured by enzyme-linked immunosorbent assay (ELISA). We show reduced p38 MAPK activation in curcumin-treated mucosal biopsies, enhanced IL-10 and reduced IL-1â. We demonstrate dose-dependent suppression of MMP-3 in CMF with curcumin. We conclude that curcumin, a naturally occurring food substance with no known human toxicity, holds promise as a novel therapy in IBD.
Fil: Epstein, Jenny. University of London; Reino Unido
Fil: Docena, Guillermo H.. University of London; Reino Unido. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: MacDonald, Thomas T.. University of London; Reino Unido
Fil: Sanderson, Ian R.. University of London; Reino Unido
Materia
Curcumin
Inflammatory bowel disease
Interleukin-10
Matrix metalloproteinase-3
p38 mitogen-activated protein kinase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/154898
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/154898
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Curcumin suppresses p38 mitogen-activated protein kinase activation, reduces IL-1β and matrix metalloproteinase-3 and enhances IL-10 in the mucosa of children and adults with inflammatory bowel diseaseEpstein, JennyDocena, Guillermo H.MacDonald, Thomas T.Sanderson, Ian R.CurcuminInflammatory bowel diseaseInterleukin-10Matrix metalloproteinase-3p38 mitogen-activated protein kinasehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Inflammatory bowel disease (IBD) is a major source of morbidity in children and adults. Its incidence is rising, particularly in young people. IBD carries a lifelong risk of cancer which is proportional to disease duration. Drug and surgical treatments rarely offer cure and often carry a high side effect burden. Dietary therapy is highly effective in selected cases of Crohn’s disease. For these reasons there is much interest in developing novel dietary treatments in IBD. Curcumin, a component of the spice turmeric, and an anti-inflammatory and anti-cancer agent, shows preclinical and clinical potential in IBD. Its mechanisms of action are unknown. Our aim was to assess the effect of curcumin on key disease mediators p38 MAPK, IL-1â, IL-10 and MMP-3 in the gut of children and adults with IBD. Colonic mucosal biopsies and myofibroblasts (CMF) from children and adults with active IBD were cultured ex vivo with curcumin. p38 MAPK, NF-êB and MMP-3 were measured by immunoblotting. IL-1â and IL-10 were measured by enzyme-linked immunosorbent assay (ELISA). We show reduced p38 MAPK activation in curcumin-treated mucosal biopsies, enhanced IL-10 and reduced IL-1â. We demonstrate dose-dependent suppression of MMP-3 in CMF with curcumin. We conclude that curcumin, a naturally occurring food substance with no known human toxicity, holds promise as a novel therapy in IBD.Fil: Epstein, Jenny. University of London; Reino UnidoFil: Docena, Guillermo H.. University of London; Reino Unido. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: MacDonald, Thomas T.. University of London; Reino UnidoFil: Sanderson, Ian R.. University of London; Reino UnidoCambridge University Press2009-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/154898Epstein, Jenny; Docena, Guillermo H.; MacDonald, Thomas T.; Sanderson, Ian R.; Curcumin suppresses p38 mitogen-activated protein kinase activation, reduces IL-1β and matrix metalloproteinase-3 and enhances IL-10 in the mucosa of children and adults with inflammatory bowel disease; Cambridge University Press; British Journal of Nutrition; 103; 6; 11-2009; 824-8320007-11451475-2662CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/british-journal-of-nutrition/article/curcumin-suppresses-p38-mitogenactivated-protein-kinase-activation-reduces-il1-and-matrix-metalloproteinase3-and-enhances-il10-in-the-mucosa-of-children-and-adults-with-inflammatory-bowel-disease/D3DF07DB9DFF1C9A0C9EABAE8B0DAFCBinfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0007114509992510info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:08Zoai:ri.conicet.gov.ar:11336/154898instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:08.566CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Curcumin suppresses p38 mitogen-activated protein kinase activation, reduces IL-1β and matrix metalloproteinase-3 and enhances IL-10 in the mucosa of children and adults with inflammatory bowel disease
title Curcumin suppresses p38 mitogen-activated protein kinase activation, reduces IL-1β and matrix metalloproteinase-3 and enhances IL-10 in the mucosa of children and adults with inflammatory bowel disease
spellingShingle Curcumin suppresses p38 mitogen-activated protein kinase activation, reduces IL-1β and matrix metalloproteinase-3 and enhances IL-10 in the mucosa of children and adults with inflammatory bowel disease
Epstein, Jenny
Curcumin
Inflammatory bowel disease
Interleukin-10
Matrix metalloproteinase-3
p38 mitogen-activated protein kinase
title_short Curcumin suppresses p38 mitogen-activated protein kinase activation, reduces IL-1β and matrix metalloproteinase-3 and enhances IL-10 in the mucosa of children and adults with inflammatory bowel disease
title_full Curcumin suppresses p38 mitogen-activated protein kinase activation, reduces IL-1β and matrix metalloproteinase-3 and enhances IL-10 in the mucosa of children and adults with inflammatory bowel disease
title_fullStr Curcumin suppresses p38 mitogen-activated protein kinase activation, reduces IL-1β and matrix metalloproteinase-3 and enhances IL-10 in the mucosa of children and adults with inflammatory bowel disease
title_full_unstemmed Curcumin suppresses p38 mitogen-activated protein kinase activation, reduces IL-1β and matrix metalloproteinase-3 and enhances IL-10 in the mucosa of children and adults with inflammatory bowel disease
title_sort Curcumin suppresses p38 mitogen-activated protein kinase activation, reduces IL-1β and matrix metalloproteinase-3 and enhances IL-10 in the mucosa of children and adults with inflammatory bowel disease
dc.creator.none.fl_str_mv Epstein, Jenny
Docena, Guillermo H.
MacDonald, Thomas T.
Sanderson, Ian R.
author Epstein, Jenny
author_facet Epstein, Jenny
Docena, Guillermo H.
MacDonald, Thomas T.
Sanderson, Ian R.
author_role author
author2 Docena, Guillermo H.
MacDonald, Thomas T.
Sanderson, Ian R.
author2_role author
author
author
dc.subject.none.fl_str_mv Curcumin
Inflammatory bowel disease
Interleukin-10
Matrix metalloproteinase-3
p38 mitogen-activated protein kinase
topic Curcumin
Inflammatory bowel disease
Interleukin-10
Matrix metalloproteinase-3
p38 mitogen-activated protein kinase
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Inflammatory bowel disease (IBD) is a major source of morbidity in children and adults. Its incidence is rising, particularly in young people. IBD carries a lifelong risk of cancer which is proportional to disease duration. Drug and surgical treatments rarely offer cure and often carry a high side effect burden. Dietary therapy is highly effective in selected cases of Crohn’s disease. For these reasons there is much interest in developing novel dietary treatments in IBD. Curcumin, a component of the spice turmeric, and an anti-inflammatory and anti-cancer agent, shows preclinical and clinical potential in IBD. Its mechanisms of action are unknown. Our aim was to assess the effect of curcumin on key disease mediators p38 MAPK, IL-1â, IL-10 and MMP-3 in the gut of children and adults with IBD. Colonic mucosal biopsies and myofibroblasts (CMF) from children and adults with active IBD were cultured ex vivo with curcumin. p38 MAPK, NF-êB and MMP-3 were measured by immunoblotting. IL-1â and IL-10 were measured by enzyme-linked immunosorbent assay (ELISA). We show reduced p38 MAPK activation in curcumin-treated mucosal biopsies, enhanced IL-10 and reduced IL-1â. We demonstrate dose-dependent suppression of MMP-3 in CMF with curcumin. We conclude that curcumin, a naturally occurring food substance with no known human toxicity, holds promise as a novel therapy in IBD.
Fil: Epstein, Jenny. University of London; Reino Unido
Fil: Docena, Guillermo H.. University of London; Reino Unido. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: MacDonald, Thomas T.. University of London; Reino Unido
Fil: Sanderson, Ian R.. University of London; Reino Unido
description Inflammatory bowel disease (IBD) is a major source of morbidity in children and adults. Its incidence is rising, particularly in young people. IBD carries a lifelong risk of cancer which is proportional to disease duration. Drug and surgical treatments rarely offer cure and often carry a high side effect burden. Dietary therapy is highly effective in selected cases of Crohn’s disease. For these reasons there is much interest in developing novel dietary treatments in IBD. Curcumin, a component of the spice turmeric, and an anti-inflammatory and anti-cancer agent, shows preclinical and clinical potential in IBD. Its mechanisms of action are unknown. Our aim was to assess the effect of curcumin on key disease mediators p38 MAPK, IL-1â, IL-10 and MMP-3 in the gut of children and adults with IBD. Colonic mucosal biopsies and myofibroblasts (CMF) from children and adults with active IBD were cultured ex vivo with curcumin. p38 MAPK, NF-êB and MMP-3 were measured by immunoblotting. IL-1â and IL-10 were measured by enzyme-linked immunosorbent assay (ELISA). We show reduced p38 MAPK activation in curcumin-treated mucosal biopsies, enhanced IL-10 and reduced IL-1â. We demonstrate dose-dependent suppression of MMP-3 in CMF with curcumin. We conclude that curcumin, a naturally occurring food substance with no known human toxicity, holds promise as a novel therapy in IBD.
publishDate 2009
dc.date.none.fl_str_mv 2009-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/154898
Epstein, Jenny; Docena, Guillermo H.; MacDonald, Thomas T.; Sanderson, Ian R.; Curcumin suppresses p38 mitogen-activated protein kinase activation, reduces IL-1β and matrix metalloproteinase-3 and enhances IL-10 in the mucosa of children and adults with inflammatory bowel disease; Cambridge University Press; British Journal of Nutrition; 103; 6; 11-2009; 824-832
0007-1145
1475-2662
CONICET Digital
CONICET
url http://hdl.handle.net/11336/154898
identifier_str_mv Epstein, Jenny; Docena, Guillermo H.; MacDonald, Thomas T.; Sanderson, Ian R.; Curcumin suppresses p38 mitogen-activated protein kinase activation, reduces IL-1β and matrix metalloproteinase-3 and enhances IL-10 in the mucosa of children and adults with inflammatory bowel disease; Cambridge University Press; British Journal of Nutrition; 103; 6; 11-2009; 824-832
0007-1145
1475-2662
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/british-journal-of-nutrition/article/curcumin-suppresses-p38-mitogenactivated-protein-kinase-activation-reduces-il1-and-matrix-metalloproteinase3-and-enhances-il10-in-the-mucosa-of-children-and-adults-with-inflammatory-bowel-disease/D3DF07DB9DFF1C9A0C9EABAE8B0DAFCB
info:eu-repo/semantics/altIdentifier/doi/10.1017/S0007114509992510
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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