Effect of nitroxyl on the hamster retinal nitridergic pathway

Autores
Sáenz, Daniel A.; Bari, Sara Elizabeth; Salido, Ezequiel Martín; Chianelli, Mónica Silvia; Rosenstein, Ruth Estela
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
There is a growing body of evidence on the role of nitric oxide (NO) in retinal physiology. Recently, interest has developed in the functional role of an alternative redox form of NO, namely nitroxyl (HNO/NO), because it is formed by a number of diverse biochemical reactions. The aim of the present report was to comparatively analyze the effect of HNO and NO on the retinal nitridergic pathway in the golden hamster. For this purpose, sodium trioxodinitrate (Angeli’s salt) and diethylammonium (Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate (DEA/NO) were used as HNO and NO releasers, respectively. Angeli’s salt and DEA/NO significantly decreased nitric oxide synthase activity. In addition, Angeli’s salt (but not DEA/NO) significantly decreased L-arginine uptake. DEA/NO significantly increased cGMP accumulation at low micromolar concentrations, while Angeli’s salt affected this parameter with a threshold concentration of 200 mM. Although Angeli’s salt and DEA/NO significantly diminished reduced glutathione and protein thiol levels in a similar way, DEA/NO was significantly more effective than AS in increasing Snitrosothiol levels. None of these compounds increased retinal lipid peroxidation. These results suggest that HNO could regulate the hamster retinal nitridergic pathway by acting through a mechanism that only partly overlaps with that involved in NO response.
Fil: Sáenz, Daniel A.. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Neuroquímica Retiniana y Oftalmología Experimental; Argentina
Fil: Bari, Sara Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina
Fil: Salido, Ezequiel Martín. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Neuroquímica Retiniana y Oftalmología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chianelli, Mónica Silvia. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Neuroquímica Retiniana y Oftalmología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rosenstein, Ruth Estela. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Neuroquímica Retiniana y Oftalmología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
nitridergic pathway
nitroxyl
Angeli´s salt
hamster
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/103270
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/103270
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of nitroxyl on the hamster retinal nitridergic pathwaySáenz, Daniel A.Bari, Sara ElizabethSalido, Ezequiel MartínChianelli, Mónica SilviaRosenstein, Ruth Estelanitridergic pathwaynitroxylAngeli´s salthamsterhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1There is a growing body of evidence on the role of nitric oxide (NO) in retinal physiology. Recently, interest has developed in the functional role of an alternative redox form of NO, namely nitroxyl (HNO/NO), because it is formed by a number of diverse biochemical reactions. The aim of the present report was to comparatively analyze the effect of HNO and NO on the retinal nitridergic pathway in the golden hamster. For this purpose, sodium trioxodinitrate (Angeli’s salt) and diethylammonium (Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate (DEA/NO) were used as HNO and NO releasers, respectively. Angeli’s salt and DEA/NO significantly decreased nitric oxide synthase activity. In addition, Angeli’s salt (but not DEA/NO) significantly decreased L-arginine uptake. DEA/NO significantly increased cGMP accumulation at low micromolar concentrations, while Angeli’s salt affected this parameter with a threshold concentration of 200 mM. Although Angeli’s salt and DEA/NO significantly diminished reduced glutathione and protein thiol levels in a similar way, DEA/NO was significantly more effective than AS in increasing Snitrosothiol levels. None of these compounds increased retinal lipid peroxidation. These results suggest that HNO could regulate the hamster retinal nitridergic pathway by acting through a mechanism that only partly overlaps with that involved in NO response.Fil: Sáenz, Daniel A.. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Neuroquímica Retiniana y Oftalmología Experimental; ArgentinaFil: Bari, Sara Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; ArgentinaFil: Salido, Ezequiel Martín. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Neuroquímica Retiniana y Oftalmología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chianelli, Mónica Silvia. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Neuroquímica Retiniana y Oftalmología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rosenstein, Ruth Estela. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Neuroquímica Retiniana y Oftalmología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaPergamon-Elsevier Science Ltd2007-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103270Sáenz, Daniel A.; Bari, Sara Elizabeth; Salido, Ezequiel Martín; Chianelli, Mónica Silvia; Rosenstein, Ruth Estela; Effect of nitroxyl on the hamster retinal nitridergic pathway; Pergamon-Elsevier Science Ltd; Neurochemistry International; 51; 6-7; 11-2007; 424-4320197-0186CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0197018607000952info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuint.2007.04.015info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:51:46Zoai:ri.conicet.gov.ar:11336/103270instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:51:46.678CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of nitroxyl on the hamster retinal nitridergic pathway
title Effect of nitroxyl on the hamster retinal nitridergic pathway
spellingShingle Effect of nitroxyl on the hamster retinal nitridergic pathway
Sáenz, Daniel A.
nitridergic pathway
nitroxyl
Angeli´s salt
hamster
title_short Effect of nitroxyl on the hamster retinal nitridergic pathway
title_full Effect of nitroxyl on the hamster retinal nitridergic pathway
title_fullStr Effect of nitroxyl on the hamster retinal nitridergic pathway
title_full_unstemmed Effect of nitroxyl on the hamster retinal nitridergic pathway
title_sort Effect of nitroxyl on the hamster retinal nitridergic pathway
dc.creator.none.fl_str_mv Sáenz, Daniel A.
Bari, Sara Elizabeth
Salido, Ezequiel Martín
Chianelli, Mónica Silvia
Rosenstein, Ruth Estela
author Sáenz, Daniel A.
author_facet Sáenz, Daniel A.
Bari, Sara Elizabeth
Salido, Ezequiel Martín
Chianelli, Mónica Silvia
Rosenstein, Ruth Estela
author_role author
author2 Bari, Sara Elizabeth
Salido, Ezequiel Martín
Chianelli, Mónica Silvia
Rosenstein, Ruth Estela
author2_role author
author
author
author
dc.subject.none.fl_str_mv nitridergic pathway
nitroxyl
Angeli´s salt
hamster
topic nitridergic pathway
nitroxyl
Angeli´s salt
hamster
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv There is a growing body of evidence on the role of nitric oxide (NO) in retinal physiology. Recently, interest has developed in the functional role of an alternative redox form of NO, namely nitroxyl (HNO/NO), because it is formed by a number of diverse biochemical reactions. The aim of the present report was to comparatively analyze the effect of HNO and NO on the retinal nitridergic pathway in the golden hamster. For this purpose, sodium trioxodinitrate (Angeli’s salt) and diethylammonium (Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate (DEA/NO) were used as HNO and NO releasers, respectively. Angeli’s salt and DEA/NO significantly decreased nitric oxide synthase activity. In addition, Angeli’s salt (but not DEA/NO) significantly decreased L-arginine uptake. DEA/NO significantly increased cGMP accumulation at low micromolar concentrations, while Angeli’s salt affected this parameter with a threshold concentration of 200 mM. Although Angeli’s salt and DEA/NO significantly diminished reduced glutathione and protein thiol levels in a similar way, DEA/NO was significantly more effective than AS in increasing Snitrosothiol levels. None of these compounds increased retinal lipid peroxidation. These results suggest that HNO could regulate the hamster retinal nitridergic pathway by acting through a mechanism that only partly overlaps with that involved in NO response.
Fil: Sáenz, Daniel A.. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Neuroquímica Retiniana y Oftalmología Experimental; Argentina
Fil: Bari, Sara Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina
Fil: Salido, Ezequiel Martín. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Neuroquímica Retiniana y Oftalmología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chianelli, Mónica Silvia. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Neuroquímica Retiniana y Oftalmología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rosenstein, Ruth Estela. Universidad de Buenos Aires. Facultad de Medicina. Laboratorio de Neuroquímica Retiniana y Oftalmología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description There is a growing body of evidence on the role of nitric oxide (NO) in retinal physiology. Recently, interest has developed in the functional role of an alternative redox form of NO, namely nitroxyl (HNO/NO), because it is formed by a number of diverse biochemical reactions. The aim of the present report was to comparatively analyze the effect of HNO and NO on the retinal nitridergic pathway in the golden hamster. For this purpose, sodium trioxodinitrate (Angeli’s salt) and diethylammonium (Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate (DEA/NO) were used as HNO and NO releasers, respectively. Angeli’s salt and DEA/NO significantly decreased nitric oxide synthase activity. In addition, Angeli’s salt (but not DEA/NO) significantly decreased L-arginine uptake. DEA/NO significantly increased cGMP accumulation at low micromolar concentrations, while Angeli’s salt affected this parameter with a threshold concentration of 200 mM. Although Angeli’s salt and DEA/NO significantly diminished reduced glutathione and protein thiol levels in a similar way, DEA/NO was significantly more effective than AS in increasing Snitrosothiol levels. None of these compounds increased retinal lipid peroxidation. These results suggest that HNO could regulate the hamster retinal nitridergic pathway by acting through a mechanism that only partly overlaps with that involved in NO response.
publishDate 2007
dc.date.none.fl_str_mv 2007-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/103270
Sáenz, Daniel A.; Bari, Sara Elizabeth; Salido, Ezequiel Martín; Chianelli, Mónica Silvia; Rosenstein, Ruth Estela; Effect of nitroxyl on the hamster retinal nitridergic pathway; Pergamon-Elsevier Science Ltd; Neurochemistry International; 51; 6-7; 11-2007; 424-432
0197-0186
CONICET Digital
CONICET
url http://hdl.handle.net/11336/103270
identifier_str_mv Sáenz, Daniel A.; Bari, Sara Elizabeth; Salido, Ezequiel Martín; Chianelli, Mónica Silvia; Rosenstein, Ruth Estela; Effect of nitroxyl on the hamster retinal nitridergic pathway; Pergamon-Elsevier Science Ltd; Neurochemistry International; 51; 6-7; 11-2007; 424-432
0197-0186
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0197018607000952
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuint.2007.04.015
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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