The circadian deadenylase nocturnin is necessary for stabilization of the iNOS mRNA in mice

Autores
Niu, Shuang; Shingle, Danielle L.; Garbarino Pico, Eduardo; Kojima, Shihoko; Gilbert, Misty; Green, Carla B.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nocturnin is a member of the CCR4 deadenylase family, and its expression is under circadian control with peak levels at night. Because it can remove poly(A) tails from mRNAs, it is presumed to play a role in post-transcriptional control of circadian gene expression, but its target mRNAs are not known. Here we demonstrate that Nocturnin expression is acutely induced by the endotoxin lipopolysaccharide (LPS). Mouse embryo fibroblasts (MEFs) lacking Nocturnin exhibit normal patterns of acute induction of TNFa and iNOS mRNAs during the first three hours following LPS treatment, but by 24 hours, while TNFa mRNA levels are indistinguishable from WT cells, iNOS message is significantly reduced 20-fold. Accordingly,analysis of the stability of the mRNAs showed that loss of Nocturnin causes a significant decrease in the half-life of the iNOS mRNA (t1/2 = 3.3 hours in Nocturnin knockout MEFs vs. 12.4 hours in wild type MEFs), while having no effect on the TNFa message. Furthermore, mice lacking Nocturnin lose the normal nighttime peak of hepatic iNOS mRNA, and have improved survival following LPS injection. These data suggest that Nocturnin has a novel stabilizing activity that plays an important role in the circadian response to inflammatory signals.
Fil: Niu, Shuang. University of Virginia; Estados Unidos
Fil: Shingle, Danielle L.. University of Virginia; Estados Unidos. University of Texas; Estados Unidos
Fil: Garbarino Pico, Eduardo. University of Virginia; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Kojima, Shihoko. University of Virginia; Estados Unidos. University of Texas; Estados Unidos
Fil: Gilbert, Misty. University of Virginia; Estados Unidos
Fil: Green, Carla B.. University of Virginia; Estados Unidos. University of Texas; Estados Unidos
Materia
Nocturnin
iNOS
Circadian Rhythms
Posttranscriptional regulation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/229758
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/229758
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The circadian deadenylase nocturnin is necessary for stabilization of the iNOS mRNA in miceNiu, ShuangShingle, Danielle L.Garbarino Pico, EduardoKojima, ShihokoGilbert, MistyGreen, Carla B.NocturniniNOSCircadian RhythmsPosttranscriptional regulationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Nocturnin is a member of the CCR4 deadenylase family, and its expression is under circadian control with peak levels at night. Because it can remove poly(A) tails from mRNAs, it is presumed to play a role in post-transcriptional control of circadian gene expression, but its target mRNAs are not known. Here we demonstrate that Nocturnin expression is acutely induced by the endotoxin lipopolysaccharide (LPS). Mouse embryo fibroblasts (MEFs) lacking Nocturnin exhibit normal patterns of acute induction of TNFa and iNOS mRNAs during the first three hours following LPS treatment, but by 24 hours, while TNFa mRNA levels are indistinguishable from WT cells, iNOS message is significantly reduced 20-fold. Accordingly,analysis of the stability of the mRNAs showed that loss of Nocturnin causes a significant decrease in the half-life of the iNOS mRNA (t1/2 = 3.3 hours in Nocturnin knockout MEFs vs. 12.4 hours in wild type MEFs), while having no effect on the TNFa message. Furthermore, mice lacking Nocturnin lose the normal nighttime peak of hepatic iNOS mRNA, and have improved survival following LPS injection. These data suggest that Nocturnin has a novel stabilizing activity that plays an important role in the circadian response to inflammatory signals.Fil: Niu, Shuang. University of Virginia; Estados UnidosFil: Shingle, Danielle L.. University of Virginia; Estados Unidos. University of Texas; Estados UnidosFil: Garbarino Pico, Eduardo. University of Virginia; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Kojima, Shihoko. University of Virginia; Estados Unidos. University of Texas; Estados UnidosFil: Gilbert, Misty. University of Virginia; Estados UnidosFil: Green, Carla B.. University of Virginia; Estados Unidos. University of Texas; Estados UnidosPublic Library of Science2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/229758Niu, Shuang; Shingle, Danielle L.; Garbarino Pico, Eduardo; Kojima, Shihoko; Gilbert, Misty; et al.; The circadian deadenylase nocturnin is necessary for stabilization of the iNOS mRNA in mice; Public Library of Science; Plos One; 6; 11; 11-2011; 26954-269631932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0026954info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0026954info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:37Zoai:ri.conicet.gov.ar:11336/229758instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:37.632CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The circadian deadenylase nocturnin is necessary for stabilization of the iNOS mRNA in mice
title The circadian deadenylase nocturnin is necessary for stabilization of the iNOS mRNA in mice
spellingShingle The circadian deadenylase nocturnin is necessary for stabilization of the iNOS mRNA in mice
Niu, Shuang
Nocturnin
iNOS
Circadian Rhythms
Posttranscriptional regulation
title_short The circadian deadenylase nocturnin is necessary for stabilization of the iNOS mRNA in mice
title_full The circadian deadenylase nocturnin is necessary for stabilization of the iNOS mRNA in mice
title_fullStr The circadian deadenylase nocturnin is necessary for stabilization of the iNOS mRNA in mice
title_full_unstemmed The circadian deadenylase nocturnin is necessary for stabilization of the iNOS mRNA in mice
title_sort The circadian deadenylase nocturnin is necessary for stabilization of the iNOS mRNA in mice
dc.creator.none.fl_str_mv Niu, Shuang
Shingle, Danielle L.
Garbarino Pico, Eduardo
Kojima, Shihoko
Gilbert, Misty
Green, Carla B.
author Niu, Shuang
author_facet Niu, Shuang
Shingle, Danielle L.
Garbarino Pico, Eduardo
Kojima, Shihoko
Gilbert, Misty
Green, Carla B.
author_role author
author2 Shingle, Danielle L.
Garbarino Pico, Eduardo
Kojima, Shihoko
Gilbert, Misty
Green, Carla B.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Nocturnin
iNOS
Circadian Rhythms
Posttranscriptional regulation
topic Nocturnin
iNOS
Circadian Rhythms
Posttranscriptional regulation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Nocturnin is a member of the CCR4 deadenylase family, and its expression is under circadian control with peak levels at night. Because it can remove poly(A) tails from mRNAs, it is presumed to play a role in post-transcriptional control of circadian gene expression, but its target mRNAs are not known. Here we demonstrate that Nocturnin expression is acutely induced by the endotoxin lipopolysaccharide (LPS). Mouse embryo fibroblasts (MEFs) lacking Nocturnin exhibit normal patterns of acute induction of TNFa and iNOS mRNAs during the first three hours following LPS treatment, but by 24 hours, while TNFa mRNA levels are indistinguishable from WT cells, iNOS message is significantly reduced 20-fold. Accordingly,analysis of the stability of the mRNAs showed that loss of Nocturnin causes a significant decrease in the half-life of the iNOS mRNA (t1/2 = 3.3 hours in Nocturnin knockout MEFs vs. 12.4 hours in wild type MEFs), while having no effect on the TNFa message. Furthermore, mice lacking Nocturnin lose the normal nighttime peak of hepatic iNOS mRNA, and have improved survival following LPS injection. These data suggest that Nocturnin has a novel stabilizing activity that plays an important role in the circadian response to inflammatory signals.
Fil: Niu, Shuang. University of Virginia; Estados Unidos
Fil: Shingle, Danielle L.. University of Virginia; Estados Unidos. University of Texas; Estados Unidos
Fil: Garbarino Pico, Eduardo. University of Virginia; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Kojima, Shihoko. University of Virginia; Estados Unidos. University of Texas; Estados Unidos
Fil: Gilbert, Misty. University of Virginia; Estados Unidos
Fil: Green, Carla B.. University of Virginia; Estados Unidos. University of Texas; Estados Unidos
description Nocturnin is a member of the CCR4 deadenylase family, and its expression is under circadian control with peak levels at night. Because it can remove poly(A) tails from mRNAs, it is presumed to play a role in post-transcriptional control of circadian gene expression, but its target mRNAs are not known. Here we demonstrate that Nocturnin expression is acutely induced by the endotoxin lipopolysaccharide (LPS). Mouse embryo fibroblasts (MEFs) lacking Nocturnin exhibit normal patterns of acute induction of TNFa and iNOS mRNAs during the first three hours following LPS treatment, but by 24 hours, while TNFa mRNA levels are indistinguishable from WT cells, iNOS message is significantly reduced 20-fold. Accordingly,analysis of the stability of the mRNAs showed that loss of Nocturnin causes a significant decrease in the half-life of the iNOS mRNA (t1/2 = 3.3 hours in Nocturnin knockout MEFs vs. 12.4 hours in wild type MEFs), while having no effect on the TNFa message. Furthermore, mice lacking Nocturnin lose the normal nighttime peak of hepatic iNOS mRNA, and have improved survival following LPS injection. These data suggest that Nocturnin has a novel stabilizing activity that plays an important role in the circadian response to inflammatory signals.
publishDate 2011
dc.date.none.fl_str_mv 2011-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/229758
Niu, Shuang; Shingle, Danielle L.; Garbarino Pico, Eduardo; Kojima, Shihoko; Gilbert, Misty; et al.; The circadian deadenylase nocturnin is necessary for stabilization of the iNOS mRNA in mice; Public Library of Science; Plos One; 6; 11; 11-2011; 26954-26963
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/229758
identifier_str_mv Niu, Shuang; Shingle, Danielle L.; Garbarino Pico, Eduardo; Kojima, Shihoko; Gilbert, Misty; et al.; The circadian deadenylase nocturnin is necessary for stabilization of the iNOS mRNA in mice; Public Library of Science; Plos One; 6; 11; 11-2011; 26954-26963
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0026954
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0026954
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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