Chagasic antibodies induce cardiac COX-2/iNOS mRNA expression with PGE2/NO production

Autores
Ganzinelli, Sabrina Belen; Borda, Enri Santiago; Joensen, Lilian; Borda, Enri Santiago
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We demonstrate that serum IgG in chagasic patients interacting with the second extracellular loop of human cardiac M2 muscarinic acetylcholine receptors (M2 mAChR) trigger the production of PGE2 and NO, that in turn induces COX-2/iNOS mRNA expression. An association between serum anti-M2 peptide IgG, anti-cardiac membrane IgG and PGE2 levels (pb0.05) in chagasic dysautonomic patients was observed. Thus, we establish that serum anti-mAChR autoantibodies and PGE2 might be considered as early markers of Chagas´ associated dysautonomia. Affinity purified anti-M2 peptide IgG from chagasic sera, while stimulating myocardial M2 mAChR, it exerts an increase on PGE2 generation and NOS activity, as well as COX-2/iNOS isoforms mRNA expression. The expression of these genes is related with phosphoinositides (PIs), cGMP accumulation and PKC activity. Inhibition of these enzymes shows that chagasic autoantibodies upregulation of COX-2/iNOS mRNA level is under the control of endogenous iNO/cGMP signalling system. These results provide a novel insight into the role that cholinoceptor antibodies play in the development of myocardial inflammation. To our knowledge, there has been no previous report showing that an antibody interacting with heart mAChR can act as expression inducer of proinflammatory mediators.
Fil: Ganzinelli, Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Borda, Enri Santiago. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Joensen, Lilian. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Materia
AUTOANTIBODIES
CHAGAS' DISEASE
INOS/COX-2 MRNA
MYOCARDIAL FUNCTION
PARASYMPATHETIC DYSAUTONOMIA
PGE2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/161940
Acceder
id CONICETDig_79a38a786e4aa9c5272423bdd2ed1349
oai_identifier_str oai:ri.conicet.gov.ar:11336/161940
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chagasic antibodies induce cardiac COX-2/iNOS mRNA expression with PGE2/NO productionGanzinelli, Sabrina BelenBorda, Enri SantiagoJoensen, LilianBorda, Enri SantiagoAUTOANTIBODIESCHAGAS' DISEASEINOS/COX-2 MRNAMYOCARDIAL FUNCTIONPARASYMPATHETIC DYSAUTONOMIAPGE2https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3We demonstrate that serum IgG in chagasic patients interacting with the second extracellular loop of human cardiac M2 muscarinic acetylcholine receptors (M2 mAChR) trigger the production of PGE2 and NO, that in turn induces COX-2/iNOS mRNA expression. An association between serum anti-M2 peptide IgG, anti-cardiac membrane IgG and PGE2 levels (pb0.05) in chagasic dysautonomic patients was observed. Thus, we establish that serum anti-mAChR autoantibodies and PGE2 might be considered as early markers of Chagas´ associated dysautonomia. Affinity purified anti-M2 peptide IgG from chagasic sera, while stimulating myocardial M2 mAChR, it exerts an increase on PGE2 generation and NOS activity, as well as COX-2/iNOS isoforms mRNA expression. The expression of these genes is related with phosphoinositides (PIs), cGMP accumulation and PKC activity. Inhibition of these enzymes shows that chagasic autoantibodies upregulation of COX-2/iNOS mRNA level is under the control of endogenous iNO/cGMP signalling system. These results provide a novel insight into the role that cholinoceptor antibodies play in the development of myocardial inflammation. To our knowledge, there has been no previous report showing that an antibody interacting with heart mAChR can act as expression inducer of proinflammatory mediators.Fil: Ganzinelli, Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Borda, Enri Santiago. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Joensen, Lilian. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaElsevier Ireland2009-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/161940Ganzinelli, Sabrina Belen; Borda, Enri Santiago; Joensen, Lilian; Borda, Enri Santiago; Chagasic antibodies induce cardiac COX-2/iNOS mRNA expression with PGE2/NO production; Elsevier Ireland; International Journal of Cardiology; 134; 2; 5-2009; 212-2230167-5273CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0167527308004373info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijcard.2008.02.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:05:49Zoai:ri.conicet.gov.ar:11336/161940instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:05:49.81CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chagasic antibodies induce cardiac COX-2/iNOS mRNA expression with PGE2/NO production
title Chagasic antibodies induce cardiac COX-2/iNOS mRNA expression with PGE2/NO production
spellingShingle Chagasic antibodies induce cardiac COX-2/iNOS mRNA expression with PGE2/NO production
Ganzinelli, Sabrina Belen
AUTOANTIBODIES
CHAGAS' DISEASE
INOS/COX-2 MRNA
MYOCARDIAL FUNCTION
PARASYMPATHETIC DYSAUTONOMIA
PGE2
title_short Chagasic antibodies induce cardiac COX-2/iNOS mRNA expression with PGE2/NO production
title_full Chagasic antibodies induce cardiac COX-2/iNOS mRNA expression with PGE2/NO production
title_fullStr Chagasic antibodies induce cardiac COX-2/iNOS mRNA expression with PGE2/NO production
title_full_unstemmed Chagasic antibodies induce cardiac COX-2/iNOS mRNA expression with PGE2/NO production
title_sort Chagasic antibodies induce cardiac COX-2/iNOS mRNA expression with PGE2/NO production
dc.creator.none.fl_str_mv Ganzinelli, Sabrina Belen
Borda, Enri Santiago
Joensen, Lilian
Borda, Enri Santiago
author Ganzinelli, Sabrina Belen
author_facet Ganzinelli, Sabrina Belen
Borda, Enri Santiago
Joensen, Lilian
author_role author
author2 Borda, Enri Santiago
Joensen, Lilian
author2_role author
author
dc.subject.none.fl_str_mv AUTOANTIBODIES
CHAGAS' DISEASE
INOS/COX-2 MRNA
MYOCARDIAL FUNCTION
PARASYMPATHETIC DYSAUTONOMIA
PGE2
topic AUTOANTIBODIES
CHAGAS' DISEASE
INOS/COX-2 MRNA
MYOCARDIAL FUNCTION
PARASYMPATHETIC DYSAUTONOMIA
PGE2
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We demonstrate that serum IgG in chagasic patients interacting with the second extracellular loop of human cardiac M2 muscarinic acetylcholine receptors (M2 mAChR) trigger the production of PGE2 and NO, that in turn induces COX-2/iNOS mRNA expression. An association between serum anti-M2 peptide IgG, anti-cardiac membrane IgG and PGE2 levels (pb0.05) in chagasic dysautonomic patients was observed. Thus, we establish that serum anti-mAChR autoantibodies and PGE2 might be considered as early markers of Chagas´ associated dysautonomia. Affinity purified anti-M2 peptide IgG from chagasic sera, while stimulating myocardial M2 mAChR, it exerts an increase on PGE2 generation and NOS activity, as well as COX-2/iNOS isoforms mRNA expression. The expression of these genes is related with phosphoinositides (PIs), cGMP accumulation and PKC activity. Inhibition of these enzymes shows that chagasic autoantibodies upregulation of COX-2/iNOS mRNA level is under the control of endogenous iNO/cGMP signalling system. These results provide a novel insight into the role that cholinoceptor antibodies play in the development of myocardial inflammation. To our knowledge, there has been no previous report showing that an antibody interacting with heart mAChR can act as expression inducer of proinflammatory mediators.
Fil: Ganzinelli, Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Borda, Enri Santiago. Universidad de Buenos Aires. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Joensen, Lilian. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología; Argentina
description We demonstrate that serum IgG in chagasic patients interacting with the second extracellular loop of human cardiac M2 muscarinic acetylcholine receptors (M2 mAChR) trigger the production of PGE2 and NO, that in turn induces COX-2/iNOS mRNA expression. An association between serum anti-M2 peptide IgG, anti-cardiac membrane IgG and PGE2 levels (pb0.05) in chagasic dysautonomic patients was observed. Thus, we establish that serum anti-mAChR autoantibodies and PGE2 might be considered as early markers of Chagas´ associated dysautonomia. Affinity purified anti-M2 peptide IgG from chagasic sera, while stimulating myocardial M2 mAChR, it exerts an increase on PGE2 generation and NOS activity, as well as COX-2/iNOS isoforms mRNA expression. The expression of these genes is related with phosphoinositides (PIs), cGMP accumulation and PKC activity. Inhibition of these enzymes shows that chagasic autoantibodies upregulation of COX-2/iNOS mRNA level is under the control of endogenous iNO/cGMP signalling system. These results provide a novel insight into the role that cholinoceptor antibodies play in the development of myocardial inflammation. To our knowledge, there has been no previous report showing that an antibody interacting with heart mAChR can act as expression inducer of proinflammatory mediators.
publishDate 2009
dc.date.none.fl_str_mv 2009-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/161940
Ganzinelli, Sabrina Belen; Borda, Enri Santiago; Joensen, Lilian; Borda, Enri Santiago; Chagasic antibodies induce cardiac COX-2/iNOS mRNA expression with PGE2/NO production; Elsevier Ireland; International Journal of Cardiology; 134; 2; 5-2009; 212-223
0167-5273
CONICET Digital
CONICET
url http://hdl.handle.net/11336/161940
identifier_str_mv Ganzinelli, Sabrina Belen; Borda, Enri Santiago; Joensen, Lilian; Borda, Enri Santiago; Chagasic antibodies induce cardiac COX-2/iNOS mRNA expression with PGE2/NO production; Elsevier Ireland; International Journal of Cardiology; 134; 2; 5-2009; 212-223
0167-5273
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0167527308004373
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijcard.2008.02.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980226062090240
score 13.004268

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